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dc.rights.licenseAtribución-NoComercial 4.0 Internacional
dc.contributorCaetano, Creucí María
dc.contributorPerera Panawalage, Indra Prasanthi
dc.contributor.authorMoreno O, Pablo E
dc.description.abstractAbstract The cassava crop Manihot esculenta crantz is the third resource more important to provide calories to the population in the marginal tropical countries of Africa, Asia and South America. However, socioeconomic factors, market conditions, pest, diseases, biotic and abiotic constraints affect cassava production of smallholders. Multiple efforts in cassava breeding program have been made, but his high heterozygous and strong inbreeding depression difficult the production of homozygous parental, causing that conventional crossings lead to a time large. For this reason the doubled haploid technology by isolated microspore culture for production of homozygous plant is an interesting alternative, reducing time and cost in hybrid production. This study was conducted in the International Center of Tropical Agriculture, we assessed cytology and cytogenetic features of microspores tetrads in TMS60444 and SM1219-9 cassava genotypes for optimize efficient protocols of doubled haploid production through evaluation of meiotic abnormalities, meiotic index and viability in microspore tetrads of donor plant used for doubled haploid production. Abnormalities during microsporogenesis like multiple nucleoli, sticky chromosomes, micronuclei, irregular spindles, dyads, triads and polyads were evidenced although in a low frequency. However, the great level of meiotic index and high microspore viability lead to conclude that these cassava genotypes are meiotically stable and can be used in breeding programs or conservation strategies.
dc.relation.ispartofUniversidad Nacional de Colombia Sede Palmira Facultad de Ciencias Agropecuarias Maestría en Ciencias Biológicas
dc.relation.ispartofMaestría en Ciencias Biológicas
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.subject.ddc57 Ciencias de la vida; Biología / Life sciences; biology
dc.subject.ddc58 Plantas / Plants
dc.titleChromosomal abnormalities during microsporogenesis in two varieties of cassava Manihot esculenta Crantz
dc.typeTrabajo de grado - Maestría
dc.relation.referencesMoreno O, Pablo E (2015) Chromosomal abnormalities during microsporogenesis in two varieties of cassava Manihot esculenta Crantz. Maestría thesis, Universidad Nacional de Colombia - sede Palmira.
dc.subject.proposalDouble haploid

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Atribución-NoComercial 4.0 InternacionalThis work is licensed under a Creative Commons Reconocimiento-NoComercial 4.0.This document has been deposited by the author (s) under the following certificate of deposit