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dc.rights.licenseAtribución-NoComercial 4.0 Internacional
dc.contributor.authorJiménez-Barreto, Jenny
dc.contributor.authorChaparro Giraldo, Alejandro
dc.contributor.authorMora-Oberlaender, Julián
dc.contributor.authorVargas-Sánchez, José Ever
dc.identifier.issnISSN: 2357-3732
dc.description.abstractSpodoptera frugiperda is one of the most common pests in maize crops, causing important losses in Colombia. The development of insect resistant crops is an alternative to conventional pest control methods. HERCULEX® I is a transgenic maize line with resistance to lepidopteran insects and tolerance to phosphinothricin herbicides, conferred by genes cry1F and pat, respectively. In Colombia, the National Federation of Cereal and Legume Growers (FENALCE) has carried out mendelian crosses between Colombian maize genotypes and HERCU- LEX® I, with the aim of integrating the genetic elements of the transgenic line into national varieties. Three hybrids were obtained which potentially carry such constructs. In the present study, the hybrid lines and their parental lines were characterized at the molecular level. Leaf tissue was tested for presence or absence of the genes cry1F and pat and their expression as mRNA and respective proteins Cry1F and Pat. Results show that the three hybrids indeed carry the HERCULEX® I constructs. RT-PCR and ELISA analysis showed transcription of the genes and presence of the proteins. An initial approach to the Freedom to Operate analysis was carried out for HERCU- LEX® I in Colombia.
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
dc.relation.ispartofAgronomía Colombiana
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.subject.ddc63 Agricultura y tecnologías relacionadas / Agriculture
dc.titleMolecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties
dc.typeArtículo de revista
dc.relation.referencesJiménez-Barreto, Jenny and Chaparro Giraldo, Alejandro and Mora-Oberlaender, Julián and Vargas-Sánchez, José Ever (2016) Molecular characterization and Freedom to Operate analysis of maize hybrids from genetically modified and Colombian varieties. Agronomía Colombiana, 34 (3). pp. 309-316. ISSN 2357-3732
dc.subject.proposalcrop improvement
dc.subject.proposalpest resistance.

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Atribución-NoComercial 4.0 InternacionalThis work is licensed under a Creative Commons Reconocimiento-NoComercial 4.0.This document has been deposited by the author (s) under the following certificate of deposit