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dc.rights.licenseAtribución-NoComercial 4.0 Internacional
dc.contributor.authorGómez Buitrago, Paola Andrea
dc.contributor.authorGonzález Correa, Carlos Augusto
dc.contributor.authorSantacoloma Osorio, Mario
dc.contributor.authorTaborda Ocampo, Gonzalo
dc.contributor.authorZezzi Arruda, Marco Aurelio
dc.date.accessioned2019-07-03T01:53:25Z
dc.date.available2019-07-03T01:53:25Z
dc.date.issued2014-09-01
dc.identifierISSN: 2357-3791
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/66313
dc.description.abstractHuman intestinal mucus essentially consists of a network of Mucin2 glycoproteins embedded in many lower molecularweight proteins. This paper contributes to the proteomic study of human intestinal mucus by comparing two sample collectionmethods (transanal irrigation and brush cytology during proctosigmoidoscopy) and analysis techniques (electrophoresis anddigestion in solution). The entire sample collection and treatment process is explained, including protein extraction, digestion anddesalination and peptide characterisation using a nanoAcquity UPLC chromatograph coupled to an HDMS spectrometer equipped with a nanoESI source. Collecting mucus via transanal irrigation provided a larger sample volume and protein concentration from a single patient. The proctosigmoidoscopy sample could be analysed via digestion in solution after depleting albumin. The analysisindicates that a simple mucus lysis method can evaluate the electrophoresis and digestion in solution techniques. Studying human intestinal mucus complexes is important because they perform two essential survival functions for humans as the first biochemical and physical defences for the gastrointestinal tract and a habitat for intestinal microbiota, which are primarily hosted in the colon and exceeds the human genetic information and cell number 100- and 10-fold (1).
dc.description.abstractHuman intestinal mucus essentially consistsof a network of Mucin2 glycoproteinsembedded in many lower molecularweight proteins. This paper contributes tothe proteomic study of human intestinalmucus by comparing two sample collectionmethods (transanal irrigation and brushcytology during proctosigmoidoscopy) andanalysis techniques (electrophoresis anddigestion in solution). The entire samplecollection and treatment process is explained,including protein extraction, digestion anddesalination and peptide characterisationusing a nanoAcquity UPLC chromatographcoupled to an HDMS spectrometer equippedwith a nanoESI source. Collecting mucus viatransanal irrigation provided a larger samplevolume and protein concentration from asingle patient. The proctosigmoidoscopysample could be analysed via digestion insolution after depleting albumin. The analysisindicates that a simple mucus lysis methodcan evaluate the electrophoresis and digestionin solution techniques. Studying humanintestinal mucus complexes is importantbecause they perform two essential survivalfunctions for humans as the first biochemicaland physical defences for the gastrointestinaltract and a habitat for intestinal microbiota,which are primarily hosted in the colon andexceeds the human genetic information andcell number 100- and 10-fold (1).
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Química
dc.relationhttps://revistas.unal.edu.co/index.php/rcolquim/article/view/53586
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química
dc.relation.ispartofRevista Colombiana de Química
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc54 Química y ciencias afines / Chemistry
dc.titleHuman intestinal mucus proteins isolated by transanal irrigation and proctosigmoidoscopy
dc.typeArtículo de revista
dc.type.driverinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.eprintshttp://bdigital.unal.edu.co/67337/
dc.relation.referencesGómez Buitrago, Paola Andrea and González Correa, Carlos Augusto and Santacoloma Osorio, Mario and Taborda Ocampo, Gonzalo and Zezzi Arruda, Marco Aurelio (2014) Human intestinal mucus proteins isolated by transanal irrigation and proctosigmoidoscopy. Revista Colombiana de Química, 43 (3). pp. 5-10. ISSN 2357-3791
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.proposaldigestion in solution
dc.subject.proposalelectrophoresis
dc.subject.proposalintestinal mucus
dc.subject.proposalproctosigmoidoscopy
dc.subject.proposalproteins
dc.subject.proposaltransanal irrigation
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTREF
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2


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Atribución-NoComercial 4.0 InternacionalThis work is licensed under a Creative Commons Reconocimiento-NoComercial 4.0.This document has been deposited by the author (s) under the following certificate of deposit