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dc.creatorLópez Ortega, Mónica Del Pilar
dc.creatorCriollo Campos, Paola Jimena
dc.creatorGómez Vargas, Ruth Milena
dc.creatorCamelo Runsinque, Mauricio
dc.creatorEstrada Bonilla, Germán
dc.creatorGarrido Rubiano, María Fernanda
dc.creatorBonilla Buitrago, Ruth
dc.date.accessioned2019-07-03T14:25:23Z
dc.date.available2019-07-03T14:25:23Z
dc.date.created2013
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/71393
dc.descriptionPhosphorus is limiting for growth of maize plants, and because of that use of fertilizers like Rock Phosphate has been proposed. However, direct use of Rock Phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and rock phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compound was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg .L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL .h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg .mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrphic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants.
dc.descriptionTitulo en español: Caracterización de bacterias diazotróficas solublizadoras de fosfato como promotoras de crecimiento en plantas de maízTitulo corto: Caracterización de bacterias diazotróficasAbstract: Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like rock phosphate has been proposed. However, direct use of rock phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and Rock Phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compounds was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg .L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL .h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg .mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrophic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants.Key words: phosphorus, indolic compounds, PGPR, maize.Resumen: El fósforo es limitante para el crecimiento de plantas de maíz y debido a eso se ha propuesto el uso de fertilizantes como la roca fosfórica. Sin embargo, el uso directo de roca fosfórica no es recomendado por su baja solubilidad, por lo que es necesario mejorarlo. En este estudio, un grupo de bacterias diazotróficas fueron evaluadas como bacterias solubilizadoras de fosfato, productoras de compuestos indólicos y sus efectos sobre el crecimiento de plantas de maíz. Cepas de los géneros Azospirillum, Azotobacter, Rhizobium y Klebsiella fueron evaluadas cuantitativamente en la solubilización de Ca3(PO4)2 y roca fosfórica como única fuente de fósforo en medio de cultivo SRS. Adicionalmente, la actividad de la enzima fosfatasa fue cuantificada a pH 5.0, 7.0 y 8.0 usando p-nitrofenil fosfato y, la producción de compuestos indólicos fue determinada por cuantificación colorimétrica. El efecto de la inoculación de las bacterias sobre plantas de maíz fue determinado en un experimento en invernadero con un diseño completamente al azar donde los pesos secos de raíz y hojas y el contenido de fósforo fueron evaluados. Los resultados mostraron que la cepa C50 produjo 107.2 mg .L-1 de fósforo disponible después de 12 días de fermentación y que la cepa AC10 tuvo la más alta actividad fosfatasa a pH 8 con 12.7 mg de p-nitrofenol mL .h-1. Todas las cepas sintetizaron compuestos indólicos y la cepa AV5 produjo la más alta cantidad con 63.03 µg .mL-1. Estas bacterias diazotróficas incrementaron la biomasa de las plantas por encima del 39 % y de la acumulación de fósforo por el 10 %. Aquí, el uso de bacterias diazotróficas solubilizadoras de fosfato puede representar una alternativa tecnológica para los sistemas de fertilización en plantas de maíz.      Palabras clave: fósforo, compuestos indólicos, PGPR, maíz.
dc.formatapplication/msword
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología
dc.relation.ispartofRevista Colombiana de Biotecnología
dc.relation.ispartofseriesRevista Colombiana de Biotecnología; Vol. 15, núm. 2 (2013); 115-123 1909-8758 0123-3475
dc.subjectbiotecnología
dc.subjectmicrobiología
dc.subjectbiotecnología
dc.subjectmicrobiología
dc.titleEffect of diazotrophic bacteria as phosphate solubilizing and indolic compound producers on maize plants
dc.typeinfo:eu-repo/semantics/article
dc.type.spaArtículo - Article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.bibliographicCitationLópez Ortega, Mónica Del Pilar and Criollo Campos, Paola Jimena and Gómez Vargas, Ruth Milena and Camelo Runsinque, Mauricio and Estrada Bonilla, Germán and Garrido Rubiano, María Fernanda and Bonilla Buitrago, Ruth (2013) Effect of diazotrophic bacteria as phosphate solubilizing and indolic compound producers on maize plants. Revista Colombiana de Biotecnología; Vol. 15, núm. 2 (2013); 115-123 1909-8758 0123-3475 .
dc.identifier.eprintshttp://bdigital.unal.edu.co/35863/
dc.identifier.eprintshttp://bdigital.unal.edu.co/35863/3/


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