Cherries cv. “Sweet Heart®” from an organic orchard in the district of Los Angeles, Bío Bío Region, Chile, were subjected to the application of the treatments: T0, control; T1, 1.5% sucralose, and T2, 2.0% sucralose. They were then packed in M.A. packaging, “View Fresh”, inside cardboard boxes of 5 k conserved for 20, 35 and 50 days at a temperature of 00.5 °C with relative humidity of 90.5%. Fruits under treatment T1, showed the lowest weight loss at 35 days of storage. This trend was maintained on day 50, when the addition of sucralose (T1 and T2), involved a minor loss of fruit weight with respect those similar under treatment T0. The fruit with sucralose showed as light decrease in pressure of pulp but always above the lower limit of recommended Durofel units. The use of sucralose was able to increase slightly the brightness of the fruit at 20 and 50 days of storage. The use of 1.5% sucralose (T1) had a favorable effect on the flavor and color at day 35. The appearance and acceptability of cherries, showed significant positive changes at the 35 days of storage, with the treatment (T1).