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dc.creatorHerrera, Juan Carlos
dc.creatorCamayo Velez, Gloria Cecilia
dc.creatorDe la Torre, Gloria
dc.creatorGaleano, Narmer Fernando
dc.creatorSalcedo, Edgar
dc.creatorDuran, Andres
dc.creatorRivera, Luis Fernando
dc.date.accessioned2019-07-03T15:25:30Z
dc.date.available2019-07-03T15:25:30Z
dc.date.created2013
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/72663
dc.descriptionThe presence of copia-like transposable elements in seven coffee (Coffea sp.) species, including the cultivated Coffea arabica, was investigated. The highly conserved domains of the reverse transcriptase (RT) present in the copia retrotransposons were amplified by PCR using degenerated primers. Fragments of roughly 300 bp were obtained and the nucleotide sequence was determined for 36 clones, 19 of which showed good quality.The deduced amino acid sequences were compared by multiple alignment analysis. The data suggested two distinct coffee RT groups, designated as CRTG1 and CRTG2. The sequence identities among the groups ranged from 52 to 60% for CRTG1 and 74 to 85% for CRTG2. The multiple alignment analysis revealed that some of the clones in CRTG1 were closely related to the representative elements present in other plant species such asBrassica napus, Populus ciliata and Picea abis. Furthermore,the chromosomal localization of the RT domains in C. arabicaand their putative ancestors was investigated by fluorescence in situ hybridization (FISH) analysis. FISH signals were observed throughout the chromosomes following a similar dispersed pattern with some localized regions exhibiting higher concentrationsof those elements, providing new evidence of their relativeconservation and stability in the coffee genome.
dc.formatapplication/pdf
dc.publisherUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
dc.relationhttp://revistas.unal.edu.co/index.php/agrocol/article/view/38707
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
dc.relation.ispartofAgronomía Colombiana
dc.relation.ispartofseriesAgronomía Colombiana; Vol. 31, núm. 3 (2013); 269-278 Agronomía Colombiana; Vol. 31, núm. 3 (2013); 269-278 2357-3732 0120-9965
dc.subjectgenetica vegetal
dc.subjectgenomica
dc.subjectLTR retrotransposons
dc.subjectreverse transcriptase
dc.subjectrepeat sequences
dc.subjectfluorescent in situ hybridization (FISH).
dc.titleIdentification and chromosomal distribution of copia-like retrotransposon sequences in the coffee (coffea l.) genome
dc.typeinfo:eu-repo/semantics/article
dc.type.spaArtículo - Article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.bibliographicCitationHerrera, Juan Carlos and Camayo Velez, Gloria Cecilia and De la Torre, Gloria and Galeano, Narmer Fernando and Salcedo, Edgar and Duran, Andres and Rivera, Luis Fernando (2013) Identification and chromosomal distribution of copia-like retrotransposon sequences in the coffee (coffea l.) genome. Agronomía Colombiana; Vol. 31, núm. 3 (2013); 269-278 Agronomía Colombiana; Vol. 31, núm. 3 (2013); 269-278 2357-3732 0120-9965 .
dc.identifier.eprintshttp://bdigital.unal.edu.co/37137/
dc.identifier.eprintshttp://bdigital.unal.edu.co/37137/2/


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