Show simple item record

dc.rights.licenseAtribución-NoComercial 4.0 Internacional
dc.contributor.authorAngel, Jorge Evelio
dc.contributor.authorHernández, Erick Geovanni
dc.contributor.authorHerrera, Néstor Andrés
dc.contributor.authorGómez, Linda Yhiset
dc.contributor.authorCastro, Ángela Patricia
dc.contributor.authorSepúlveda, Adriana Milena
dc.contributor.authorEbratt, Everth Emilio
dc.date.accessioned2019-07-03T16:39:09Z
dc.date.available2019-07-03T16:39:09Z
dc.date.issued2014-03-26
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/73652
dc.description.abstractFour DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008).
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
dc.relationhttp://revistas.unal.edu.co/index.php/agrocol/article/view/40544
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
dc.relation.ispartofAgronomía Colombiana
dc.relation.ispartofseriesAgronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.titleComparison of dna extraction methods for detection of citrus huanglongbing in colombia
dc.typeArtículo de revista
dc.type.driverinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.eprintshttp://bdigital.unal.edu.co/38128/
dc.relation.referencesAngel, Jorge Evelio and Hernández, Erick Geovanni and Herrera, Néstor Andrés and Gómez, Linda Yhiset and Castro, Ángela Patricia and Sepúlveda, Adriana Milena and Ebratt, Everth Emilio (2014) Comparison of dna extraction methods for detection of citrus huanglongbing in colombia. Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965 .
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.proposalBiologia molecular
dc.subject.proposalAgronomia
dc.subject.proposalCandidatus Liberibacter
dc.subject.proposalDiaphorina citri
dc.subject.proposalPsyllidae
dc.subject.proposalvectors.
dc.subject.proposalFitopatología
dc.subject.proposalentomología
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTREF
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial 4.0 InternacionalThis work is licensed under a Creative Commons Reconocimiento-NoComercial 4.0.This document has been deposited by the author (s) under the following certificate of deposit