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Production and purification of igy antibodies as a novel tool to purify the nr1 subunit of nmda recepto
dc.rights.license | Atribución-NoComercial 4.0 Internacional |
dc.contributor.author | Reyes Montaño, Edgar Antonio |
dc.contributor.author | Lareo, Leonardo |
dc.contributor.author | Pérez, Gerardo |
dc.date.accessioned | 2019-06-28T09:48:00Z |
dc.date.available | 2019-06-28T09:48:00Z |
dc.date.issued | 2012 |
dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/41066 |
dc.description.abstract | Producing polyclonal antibodies (IgY) inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit). We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassettes)were designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R |
dc.format.mimetype | application/pdf |
dc.language.iso | spa |
dc.publisher | Universidad Nacional de Colombia |
dc.relation | http://revistas.unal.edu.co/index.php/rcolquim/article/view/30306 |
dc.relation.ispartof | Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química |
dc.relation.ispartof | Revista Colombiana de Química |
dc.relation.ispartofseries | Revista Colombiana de Química; Vol. 41, núm. 1 (2012); 31-45 0120-2804 |
dc.rights | Derechos reservados - Universidad Nacional de Colombia |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ |
dc.title | Production and purification of igy antibodies as a novel tool to purify the nr1 subunit of nmda recepto |
dc.type | Artículo de revista |
dc.type.driver | info:eu-repo/semantics/article |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.identifier.eprints | http://bdigital.unal.edu.co/31163/ |
dc.relation.references | Reyes Montaño, Edgar Antonio and Lareo, Leonardo and Pérez, Gerardo (2012) Production and purification of igy antibodies as a novel tool to purify the nr1 subunit of nmda recepto. Revista Colombiana de Química; Vol. 41, núm. 1 (2012); 31-45 0120-2804 . |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content | Text |
dc.type.redcol | http://purl.org/redcol/resource_type/ART |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 |
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