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Caracterización de perfiles de elementos genéticos plasmídicos de aislamientos colombianos de Providencia rettgeri, causantes de IAAS. Obtenidos del Instituto Nacional de Salud, durante el periodo 2015-2016

dc.rights.licenseAtribución-SinDerivadas 4.0 Internacional
dc.contributor.advisorReguero Reza, Ma. Teresa Jesús
dc.contributor.advisorBarreto-Hernández, Emiliano
dc.contributor.authorRojas Suárez, Laura Viviana
dc.date.accessioned2020-07-15T19:45:55Z
dc.date.available2020-07-15T19:45:55Z
dc.date.issued2019
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/77779
dc.description.abstractProvidencia rettgeri is a generally ubiquitous Gram negative bacillus recognized for primarily causing infections at the urinary tract level and other affected infections at the respiratory tract level and gastrointestinal diseases. However, it has gained relevance in the last decade due to several reports of low antimicrobial sensitivity due to the acquisition of determining genes such as blaNDM_1. The acquisition of highly divergent extrachromosomal genetic elements have provided him with a genetic arsenal present in other Gram-negative bacteria through horizontal gene transfer. Thus, P. rettgeri has become an emerging nosocomial pathogen that has spread throughout the planet. Therefore, the present study set out to characterize profiles of resistance plasmid genetic elements present in 28 P. rettgeri isolates that present a multidrug-resistant phenotype, through a biocomputer workflow based on five different methodologies that allowed the transmission of sequences possibly associated with plasmids. These Sequences were obtained from short readings acquired from a previous complete genome (WGS) sequence of each of the isolates. This study determined the presence of plasmids in all the isolates, and 568 possibly plasmid sequences were obtained, where 41 genes associated with antimicrobial resistance were identified (35% of genes associated with complete genome resistance), where the majority of the devices a mechanism of degradation and modification of antibiotics. Likewise, the resistance profiles associated with possible plasmid sequences were defined for each of the isolates and the divergence presented by them was noted.
dc.description.abstractProvidencia rettgeri es un bacilo Gram negativo generalmente ubicuo reconocido por causar principalmente infecciones a nivel de tracto urinario y otras infecciones aisladas a nivel de tracto respiratorio y enfermedades gastrointestinales. No obstante ha cobrado relevancia en la última década debido a varios reportes de baja sensibilidad a antimicrobianos por la adquisición de genes determinantes como blaNDM_1. La adquisición de elementos genéticos extracromosomales altamente divergentes le han proporcionado un arsenal genético presente en otras bacterias Gram negativas a través de la transferencia horizontal de genes. Así, P. rettgeri se ha convertido en un patógeno nosocomial emergente que se ha extendido por todo el planeta. Por lo anterior, el presente estudio se propuso caracterizar perfiles de elementos genéticos plasmídicos de resistencia presentes en 28 aislamientos de P. rettgeri que presentan un fenotipo multirresistente, a través de un flujo de trabajo bionformático basado en cinco metodólogas distintas que permitieron la obtención de secuencias posiblemente asociadas a plásmidos. Estas Secuencias fueron obtenidas a partir lecturas cortas adquiridas de una previa secuenciación del genoma completo (WGS) de cada uno de los aislamientos. Este estudio logró determinar la presencia de plásmidos en todos los aislamientos, así mismo se obtuvieron 568 secuencias posiblemente plasmídicas en donde se identificaron 41 genes asociados a la resistencia a antimicrobianos (el 35% de genes asociados a la resistencia del genoma completo), donde la mayoría tenían un mecanismo de degradación y modificación de antibióticos. Igualmente se definieron los perfiles de resistencia asociados a posibles secuencias plasmídicas para cada uno de los aislamientos y se advirtió la divergencia presentada por ellas.
dc.format.extent144
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.relationAnálisis comparativo de los elementos genómicos de resistencia a antibióticos betalactámicos en cepas colombianas de Providencia rettgeri durante el período 2015 – 2016
dc.relationComparación de perfiles de Elementos genéticos Plasmídicos de aislamientos clínicos de Acinetobacter baumannii provenientes en el periodo 2012-2015 del Instituto Nacional de Salud, mediante secuenciación de genoma completo
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/
dc.subject.ddc000 - Ciencias de la computación, información y obras generales
dc.subject.ddc570 - Biología
dc.titleCaracterización de perfiles de elementos genéticos plasmídicos de aislamientos colombianos de Providencia rettgeri, causantes de IAAS. Obtenidos del Instituto Nacional de Salud, durante el periodo 2015-2016
dc.typeOtro
dc.rights.spaAcceso abierto
dc.description.additionalMagíster en Ciencias - Microbiología. Linea de Investigación: Epidemiología Molecular de Agentes Infecciosos
dc.type.driverinfo:eu-repo/semantics/other
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.contributor.corporatenameInstituto Nacional de Salud
dc.contributor.researchgroupBioinformática
dc.description.degreelevelMaestría
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.proposalProvidencia rettgeri
dc.subject.proposalProvidencia rettgeri
dc.subject.proposalWhole Genome Sequencing (WGS)
dc.subject.proposalSecuenciación de Genoma Completo (WGS)
dc.subject.proposalPlásmidos
dc.subject.proposalPlasmids
dc.subject.proposalContigs
dc.subject.proposalContigs
dc.subject.proposalPipeline
dc.subject.proposalPipeline
dc.subject.proposalAntimicrobial Resistance (AMR)
dc.subject.proposalResistencia a Antimicrobianos (RAM)
dc.subject.proposalPerfil genotípico de resistencia
dc.subject.proposalGenotypic resistance perfil
dc.type.coarhttp://purl.org/coar/resource_type/c_1843
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2


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