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Expresión del receptor TRPV1 en celulas similares a odontoblastos humanos: una alternativa terapéutica para el dolor y la sensibilidad dentinal

dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.contributor.advisorBaldión Elorza, Paula Alejandra
dc.contributor.authorLatorre Mora, Karen Lorena
dc.date.accessioned2021-02-09T16:38:05Z
dc.date.available2021-02-09T16:38:05Z
dc.date.issued2020-08-18
dc.identifier.citationLatorre Mora, K. L. (2020). Expresión del receptor TRPV1 en celulas similares a odontoblastos humanos: una alternativa terapéutica para el dolor y la sensibilidad dentinal [Tesis de maestría, Universidad Nacional de Colombia]. Repositorio Institucional.
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/79164
dc.description.abstractDentin hypersensitivity and dental pain are a frequent reason for consultation in dentistry; however, its pathophysiology has not yet been fully clarified. Previous findings have made it possible to relate the cellular sensory capacity with the activation of the polymodal receptor TRPV1, responsible for the nociceptive response and whose desensitization could cause analgesia. For this reason, the objective of this was to determine the expression, localization and functional activity of TRPV1 in human odontoblast-like cells (hOLCs). The human dental pulp stem cells (hDPSCs) were obtained and characterized and subjected to odontogenic differentiation for 7, 14 and 21 days; and its phenotype was evaluated by immunofluorescence for the odontogenic markers dentinal matrix protein 1 (DMP-1) and dentin sialoprotein (DSP). TRPV1 gene expression was estimated by RT-qPCR and its location was established by immunofluorescence. The EC50 of Eugenol (EUG) and Capsaicin (CAP) was determined and the activation of the receptor was evaluated under chemical, thermal and under changes in pH stimulation. For the statistical analysis, a oneway ANOVA was used with a Tukey post hoc test (p <0.05). After establishing the expression and membrane localization of TRPV1 in hOLCs, its chemical activation with EUG and CAP, its thermal at ≥ 43 °C and its activation with acidic pH <6 or basic ―between 9 and 12― were demonstrated. Receptor desensitization was achieved after 20 min exposure to concentrations of 603.5 and 1000 µM of EUG. These findings represent a contribution to the construction of a pulp pain study model aimed at offering therapeutic alternatives for these pathologies.
dc.description.abstractLa hipersensibilidad dentinal y el dolor dental son motivo de consulta frecuente en odontología; sin embargo, aún no se ha esclarecido totalmente su fisiopatología. Hallazgos previos han permitido relacionar la capacidad sensitiva celular con la activación del receptor polimodal TRPV1, responsable de la respuesta nociceptiva y cuya desensibilización podría provocar analgesia. Por esta razón, el objetivo de este estudio fue determinar la expresión, localización y actividad funcional de TRPV1 en células similares a odontoblastos humanos (hOLCs). Se obtuvieron células madre mesenquimales (hDPSCs) que fueron caracterizadas y sometidas a diferenciación odontogénica por 7, 14 y 21 días; y se evaluó su fenotipo mediante inmunofluorescencia para los marcadores odontogénicos proteína de matriz dentinal 1 (DMP-1) y sialoproteína dentinal (DSP). Se estimó la expresión génica de TRPV1 mediante RT-qPCR y la localización del mismo se estableció mediante inmunofluorescencia. Se determinó la CE50 de Eugenol (EUG) y Capsaicina (CAP) y se evaluó la activación del receptor ante estimulación química, térmica y bajo exposición a distintos pH. Para el análisis estadístico se utilizó un ANOVA de una vía con una prueba post hoc de Tukey (p<0.05). Tras establecer en hOLCs la expresión y localización membranal de TRPV1, se demostró su activación química con EUG y CAP, su activación térmica a ≥ 43 °C, y su activación con pH ácidos <6 o básicos ―entre 9 y 12―. La desensibilización del receptor se logró tras una exposición de 20 min a concentraciones de 603,5 y 1000 μM de EUG. Estos hallazgos representan un aporte para la construcción de un modelo de estudio del dolor pulpar orientado a ofrecer alternativas terapéuticas para estas patologías.
dc.description.sponsorshipConvocatoria para el fortalecimiento de alianzas interdisciplinarias y creación artística de la sede Bogotá de la Universidad Nacional de Colombia, 2018
dc.format.extent88
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.rightsDerechos reservados - Universidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc615 - Farmacología y terapéutica
dc.titleExpresión del receptor TRPV1 en celulas similares a odontoblastos humanos: una alternativa terapéutica para el dolor y la sensibilidad dentinal
dc.typeOtro
dc.rights.spaAcceso abierto
dc.description.projectEXPRESION DEL RECEPTOR DE DOLOR TRPV1 EN ODONTOBLASTOS HUMANOS INMORTALIZADOS
dc.description.additionalLínea de Investigación: Medicina Oral.
dc.type.driverinfo:eu-repo/semantics/other
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Farmacología
dc.contributor.researchgroupInvestigación Básica y Aplicada en Odontología
dc.description.degreelevelMaestría
dc.publisher.departmentDepartamento de Farmacia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
dc.relation.referencesAlmushayt, A., Narayanan, K., Zaki, A. E., & George, A. (2006). Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts. Gene Therapy, 13(7), 611–620. https://doi.org/10.1038/sj.gt.3302687
dc.relation.referencesBaldión, P. A., Velandia-romero, M. L., & Castellanos, J. E. (2018). Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation. International Journal Cell Biology. Article ID 6853189.
dc.relation.referencesBekes, K., John, M. T., Schaller, H. G., & Hirsch, C. (2009). Oral health-related quality of life in patients seeking care for dentin hypersensitivity. Journal of Oral Rehabilitation, 36(1), 45–51. https://doi.org/10.1111/j.1365-2842.2008.01901.x
dc.relation.referencesBhave, G., Zhu, W., Wang, H., Brasier, D. J., Oxford, G. S., & Gereau, R. W. (2002). cAMP-Dependent Protein Kinase Regulates Desensitization of the Capsaicin Receptor ( VR1 ) by Direct Phosphorylation. Neuron, 35, 721–731. https:// 10.1016/s0896-6273(02)00802-4
dc.relation.referencesBleicher, F. (2014). Odontoblast physiology. Experimental Cell Research, 325(2), 65–71. https://doi.org/10.1016/j.yexcr.2013.12.012
dc.relation.referencesChang, B., Svoboda, K. K. H., & Liu, X. (2019). Cell polarization: From epithelial cells to odontoblasts. European Journal of Cell Biology, 98(1), 1–11. https://doi.org/10.1016/j.ejcb.2018.11.003
dc.relation.referencesCharan Raja, M. R. (2015). Versatile and Synergistic Potential of Eugenol: A Review. Pharmaceutica Analytica Acta, 06(05). https://doi.org/10.4172/2153-2435.1000367
dc.relation.referencesChen, S., Chen, L., Jahangiri, A., Chen, B., Wu, Y., Chuang, H. H. MacDougall, M. (2008). Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. Archives of Oral Biology, 53(9), 879–889. https://doi.org/10.1016/j.archoralbio.2008.05.005
dc.relation.referencesCho, Y. D., Yoon, W. J., Woo, K. M., Baek, J. H., Park, J. C., & Ryoo, H. M. (2010). The canonical BMP signaling pathway plays a crucial part in stimulation of dentin sialophosphoprotein expression by BMP-2. Journal of Biological Chemistry, 285(47), 36369–36376. https://doi.org/10.1074/jbc.M110.103093
dc.relation.referencesChung, G., & Oh, S. (2013). TRP channels in dental pain. The Open Pain Journal, 31–36. https://doi.org/10.2174/1876386301306010031
dc.relation.referencesChung, M.-K. (2013). Targeting trp channels for pain relief: TRPV1 and beyond. Open Pain Journal, 6(SPEC.ISSUE.1), 8–9.https://doi.org/10.2174/1876386301306010008
dc.relation.referencesCohen. (2011). Vias de la Pulpa. In S. C. Kenneth M. Hargreaves, Louis.H. Berman (Ed.), Cohen’s Pathways of the pulp (10 Edition).
dc.relation.referencesCouve, E., Osorio, R., & Schmachtenberg, O. (2013). The amazing odontoblast: Activity,autophagy, and aging. Journal of Dental Research, 92(9), 765–772. https://doi.org/10.1177/0022034513495874
dc.relation.referencesda Rosa, W. L.O. Piva, E. da Silva, A. F. (2018). Disclosing the physiology of pulp tissue for vital pulp therapy. International Endodontic Journal, 51(8), 829–846. https://doi.org/10.1111/iej.12906
dc.relation.referencesDhaka, A., Uzzell, V., Dubin, A. E., Mathur, J., Petrus, M., Bandell, M., & Patapoutian, A. (2009). TRPV1 Is Activated by Both Acidic and Basic pH. The Journal of Neuroscience, 29(1), 153–158. https://doi.org/10.1523/JNEUROSCI.4901-08.2009
dc.relation.referencesDominici, M., Blanc, K. Le, Mueller, I., Marini, F. C., Krause, D. S., Deans, R. J. Horwitz, E. M. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells . The International Society for Cellular Therapy position statement. Cytotherapy, 8(4), 315–317. https://doi.org/10.1080/14653240600855905
dc.relation.referencesEl Karim, I. A., Linden, G. J., Curtis, T. M., About, I., McGahon, M. K., Irwin, C. R., & Lundy, F. T. (2011). Human odontoblasts express functional thermo-sensitive TRP channels: Implications for dentin sensitivity. Pain, 152(10), 2211–2223. https://doi.org/10.1016/j.pain.2010.10.016
dc.relation.referencesFattori, V., Hohmann, M. S. N., Rossaneis, A. C., Pinho-Ribeiro, F. A., & Verri, W. A. (2016). Capsaicin: Current understanding of its mechanisms and therapy of pain and other pre-clinical and clinical uses. Molecules, 21(7), 1–33. https://doi.org/10.3390/molecules21070844
dc.relation.referencesFlegel, C., Schöbel, N., Altmüller, J., Becker, C., Tannapfel, A., Hatt, H., & Gisselmann, G. (2015). RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors. PLoS ONE, 10(6). https://doi.org/10.1371/journal.pone.0128951
dc.relation.referencesFluo-4, AM, cell permeant.(2019, 25 de Noviembre). https://www.thermofisher.com/order/catalog/product/F14201#/F14201
dc.relation.referencesFu, H., Fang, P., Zhou, H. Y., Zhou, J., Yu, X. W., Ni, M., Hu, Z. L. (2016). Acid-sensing ion channels in trigeminal ganglion neurons innervating the orofacial region contribute to orofacial inflammatory pain. Clinical and Experimental Pharmacology and Physiology, 43(2), 193–202. https://doi.org/10.1111/1440-1681.12510
dc.relation.referencesHan, N., Zheng, Y., Li, R., Li, X., Zhou, M., Niu, Y., & Zhang, Q. (2014). β-catenin enhances odontoblastic differentiation of dental pulp cells through activation of Runx2. PLoS ONE, 9(2), 1–10. https://doi.org/10.1371/journal.pone.0088890
dc.relation.referencesHermanstyne T.O., Markowitz K., Fan L., & M. S. G. (2008). Mechanotransducers in Rat Pulpal Afferents. 31(9), 1713–1723.
dc.relation.referencesHuang, S. M., Bisogno, T., Trevisani, M., Al-Hayani, A., De Petrocellis, L., Fezza, F., Di Marzo, V. (2002). An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. Proceedings of the National Academy of Sciences, 99(12), 8400–8405. https://doi.org/10.1073/pnas.122196999
dc.relation.referencesIslam, S. (2011). Transient Receptor Potential Channels. https://doi.org/10.1007/978-94-007-0265-3
dc.relation.referencesKawashima, N., & Okiji, T. (2016). Odontoblasts: Specialized hard-tissue-forming cells in the dentin-pulp complex. Congenital Anomalies, 56(4), 144–153. https://doi.org/10.1111/cga.12169
dc.relation.referencesKim, J., & Park, J. (2017). Dentin hypersensitivity and emerging concepts for treatments. Journal of Oral Biosciences, 59(4), 211–217. https://doi.org/10.1016/j.job.2017.09.001
dc.relation.referencesKim, T. H., Bae, C. H., Lee, J. Y., Lee, J. C., Ko, S. O., Chai, Y., & Cho, E. S. (2015).Temporo-spatial requirement of Smad4 in dentin formation. Biochemical and Biophysical Research Communications, 459(4), 706–712. https://doi.org/10.1016/j.bbrc.2015.03.014
dc.relation.referencesKim, T. H., Lee, J. Y., Baek, J. A., Lee, J. C., Yang, X., Taketo, M. M., Cho, E. S. (2011). Constitutive stabilization of ß-catenin in the dental mesenchyme leads to excessive dentin and cementum formation. Biochemical and Biophysical Research Communications, 412(4), 549–555. https://doi.org/10.1016/j.bbrc.2011.07.116
dc.relation.referencesKim, Y. S., Jung, H. K., Kwon, T. K., Kim, C. S., Cho, J. H., Ahn, D. K., & Bae, Y. C. (2012). Expression of transient receptor potential Ankyrin 1 in human dental pulp. Journal of Endodontics, 38(8), 1087–1092.https://doi.org/10.1016/j.joen.2012.04.024
dc.relation.referencesKwon, M., Baek, S. H., Park, C.-K., Chung, G., & Oh, S. B. (2014a). Single-cell RT-PCR and immunocytochemical detection of mechanosensitive transient receptor potential channels in acutely isolated rat odontoblasts. Archives of Oral Biology, 59(12), 1266–1271. https://doi.org/10.1016/j.archoralbio.2014.07.016
dc.relation.referencesKwon, M., Baek, S. H., Park, C. K., Chung, G., & Oh, S. B. (2014b). Single-cell RT-PCR and immunocytochemical detection of mechanosensitive transient receptor potential channels in acutely isolated rat odontoblasts. Archives of Oral Biology, 59(12), 1266–1271. https://doi.org/10.1016/j.archoralbio.2014.07.016
dc.relation.referencesLadrón-de-guevara, E., Dominguez, L., Rangel-yescas, G. E., Daniel, A., Torres-larios, A., Rosenbaum, T., Fernández-velasco, D. A. (2020). The contribution of the ankyrin repeat domain of TRPV1 as a thermal module. Biophysical Journal, 118(4), 836– 845. https://doi.org/10.1016/j.bpj.2019.10.041
dc.relation.referencesLangenbach, F., & Handschel, J. (2013). Effects of dexamethasone, ascorbic acid and β glycerophosphate on the osteogenic differentiation of stem cells in vitro. Stem Cell Research and Therapy, 4(5), 1. https://doi.org/10.1186/scrt328
dc.relation.referencesLi, W., Chen, L., Chen, Z., Wu, L., Feng, J., Wang, F., Chen, S. (2017). Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation. Scientific Reports, 7(1), 1–18. https://doi.org/10.1038/s41598-017-00339-w
dc.relation.referencesLi, Y., Lü, X., Sun, X., Bai, S., Li, S., & Shi, J. (2011). Odontoblast-like cell differentiation and dentin formation induced with TGF-β1. Archives of Oral Biology, 56(11), 1221– 1229. https://doi.org/10.1016/j.archoralbio.2011.05.002
dc.relation.referencesLiedtke, W. B., & Heller, S. (2007). TRP ion channel function in sensory transduction and cellular signaling cascades. CRC/Taylor & Francis.
dc.relation.referencesLishko, P. V, Procko, E., Jin, X., Phelps, C. B., & Gaudet, R. (2007). Article The Ankyrin Repeats of TRPV1 Bind Multiple Ligands and Modulate Channel Sensitivity. Neuron, 54, 905–918. https://doi.org/10.1016/j.neuron.2007.05.027
dc.relation.referencesLiu, B., Zhang, C., & Qin, F. (2005). Functional Recovery from Desensitization of Vanilloid Receptor TRPV1 Requires Resynthesis of Phosphatidylinositol 4 , 5-Bisphosphate. The Journal of Neuroscience, 25(19), 4835–4843. https://doi.org/10.1523/JNEUROSCI.1296-05.2005
dc.relation.referencesMagloire, H., Couble, M. L., Thivichon-Prince, B., Maurin, J. C., & Bleicher, F. (2009). Odontoblast: A mechano-sensory cell. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 312(5), 416–424. https://doi.org/10.1002/jez.b.21264
dc.relation.referencesMesentier-Louro L.A, Zaverucha-Do-Valle C, Da Silva A.J, Dos-santos G., Gubert F., Padilha A.B, Torres A.L, Paredes B.,..Santago M. (2014). Distribution of mesenchymal stem cells and effects on neuronal survival and axon regeneration after optic nerve crush and cell therapy. PLoS One, 9(10). https://doi:10.1371/journal.pone.0110722
dc.relation.referencesMinisterio de Salud. Resolucion Numero 8430 de 1993., Octubre (1993). Por la cual se establecen las normas científicas, técnicas y administrativas para la investigación en salud. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/RESOLUCI ON-8430-DE-1993.PDF
dc.relation.referencesMohapatra, Durga P, & Nau, C. (2003). Desensitization of Capsaicin-activated Currents in the Vanilloid Receptor TRPV1 Is Decreased by the Cyclic AMP-dependent Protein Kinase Pathway *. The Journal of Biological Chemistry, 278(50), 50080–50090. https://doi.org/10.1074/jbc.M306619200
dc.relation.referencesMoran, M. M., McAlexander, M. A., Bíró, T., & Szallasi, A. (2011). Transient receptor potential channels as therapeutic targets. Nature Reviews Drug Discovery, 10(8), 601–620. https://doi.org/10.1038/nrd3456
dc.relation.referencesNie, X., Luukko, K., & Kettunen, P. (2006). BMP signalling in craniofacial development. International Journal of Developmental Biology, 50(6), 511–521. https://doi.org/10.1387/ijdb.052101xn
dc.relation.referencesNumazaki, M., Tominaga, T., Takeuchi, K., Murayama, N., Toyooka, H., & Tominaga, M. (2003). Structural determinant of TRPV1 desensitization interacts with calmodulin. PNAS, 100(13), 8002–8006
dc.relation.referencesOkumura, R., Shima, K., Muramatsu, T., Nakagawa, K., Shimono, M., Suzuki, T., Shibukawa, Y. (2005). The odontoblast as a sensory receptor cell? The expression of TRPV1 (VR-1) channels. Arch Histol Cytol, Vol. 68, pp. 251–257. https://doi.org/JST.JSTAGE/aohc/68.251 [pii]
dc.relation.referencesPalkar, R., Lippoldt, E. K., & McKemy, D. D. (2015). The molecular and cellular basis of thermosensation in mammals. Current Opinion in Neurobiology, 34, 14–19. https://doi.org/10.1016/j.conb.2015.01.010
dc.relation.referencesPark, C. K., Mi, S. K., Fang, Z., Hai, Y. L., Sung, J. J., Choi, S. Y., Seog, B. O. (2006). Functional expression of thermo-transient receptor potential channels in dental primary afferent neurons: Implication for tooth pain. Journal of Biological Chemistry, 281(25), 17304–17311. https://doi.org/10.1074/jbc.M511072200
dc.relation.referencesPriyam Jani, Chao Liu, Hua Zhang, Khaled Younes, M. D. B., & Qin, and C. (2018). The Role of Bone Morphogenetic Proteins 2 and 4 in Mouse Dentinogenesis. Arch Oral Biol, 90, 33–39. https://doi.org/10.1016/j.physbeh.2017.03.040
dc.relation.referencesQin, C., Baba, O., & Butler, W. T. (2004). Post - translational m odifications of sibling proteins and their roles in osteogenesis and dentinogenesis. Critical Reviews in Oral Biology & Medicine.15(3), 126–136.
dc.relation.referencesRathee, P. K., Distler, C., Obreja, O., Neuhuber, W., Wang, G. K., Wang, S., … Kress, M. (2002). PKA / AKAP / VR-1 Module : A Common Link of G s -Mediated Signaling to Thermal Hyperalgesia. The Journal of Neuroscience, 22(11), 4740–4745.
dc.relation.referencesRosenbaum, T., Gordon-shaag, A., Munari, M., & Gordon, S. E. (2004). Ca 2 ϩ / Calmodulin Modulates TRPV1 Activation by Capsaicin The Journal of General Physiology. 123(January). https://doi.org/10.1085/jgp.200308906
dc.relation.referencesRoth, V. (2006). Retrieved from Doubling Time Computing website: http://www.doublingtime.com/compute.php
dc.relation.referencesSato, M., Furuya, T., Kimura, M., Kojima, Y., Tazaki, M., Sato, T., & Shibukawa, Y. (2015). Intercellular odontoblast communication via ATP mediated by pannexin-1 channel and phospholipase C-coupled receptor activation. Frontiers in Physiology,6(NOV). https://doi.org/10.3389/fphys.2015.00326
dc.relation.referencesSato, M., Sobhan, U., Tsumura, M., Kuroda, H., Soya, M., Masamura, A., Shibukawa, Y. (2013). Hypotonic-induced stretching of plasma membrane activates transient receptor potential vanilloid channels and sodium-calcium exchangers in mouse odontoblasts. Journal of Endodontics, 39(6), 779–787. https://doi.org/10.1016/j.joen.2013.01.012
dc.relation.referencesSchefe, J. H., Lehmann, K. E., Buschmann, I. R., Unger, T., & Funke-Kaiser, H. (2006). Quantitative real-time RT-PCR data analysis: Current concepts and the novel “gene expression’s C T difference” formula. Journal of Molecular Medicine, 84(11), 901– 910. https://doi.org/10.1007/s00109-006-0097-6
dc.relation.referencesScheller, E. L., Chang, J., & Wang, C. Y. (2008). Wnt/β-catenin inhibits dental pulp stem cell differentiation. Journal of Dental Research, 87(2), 126–130. https://doi.org/10.1177/154405910808700206
dc.relation.referencesShibukawa, Y., Sato, M., Kimura, M., Sobhan, U., Shimada, M., Nishiyama, A., … Tazaki, M. (2015). Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction. Pflugers Archiv European Journal of Physiology, 467(4), 843–863. https://doi.org/10.1007/s00424-014-1551-x
dc.relation.referencesSolé-Magdalena, A., Martínez-Alonso, M., Coronado, C. A., Junquera, L. M., Cobo, J., & Vega, J. A. (2018). Molecular basis of dental sensitivity: The odontoblasts are multisensory cells and express multifunctional ion channels. Annals of Anatomy, 215, 20–29. https://doi.org/10.1016/j.aanat.2017.09.006
dc.relation.referencesSolé-Magdalena, A., Revuelta, E. G., Menénez-Díaz, I., Calavia, M. G., Cobo, T., García Suárez, O., … Vega, J. A. (2011). Human odontoblasts express transient receptor protein and acid-sensing ion channel mechanosensor proteins. Microscopy Research and Technique, 74(5), 457–463. https://doi.org/10.1002/jemt.20931
dc.relation.referencesSon, A. R., Yang, Y. M., Hong, J. H., Lee, S. I., Shibukawa, Y., & Shin, D. M. (2009). Odontoblast TRP channels and thermo/mechanical transmission. Journal of Dental Research, 88(11), 1014–1019. https://doi.org/10.1177/0022034509343413
dc.relation.referencesStory, G. M., Peier, A. M., Reeve, A. J., Eid, S. R., Mosbacher, J., Hricik, T. R., … Patapoutian, A. (2003). ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell, 112(6), 819–829. https://doi.org/10.1016/S0092-8674(03)00158-2
dc.relation.referencesTazawa, K., Ikeda, H., Kawashima, N., & Okiji, T. (2017). Transient receptor potential melastatin (TRPM) 8 is expressed in freshly isolated native human odontoblasts. Archives of Oral Biology, 75, 55–61. https://doi.org/10.1016/j.archoralbio.2016.12.007
dc.relation.referencesThivichon-Prince, B., Couble, M. L., Giamarchi, A., Delmas, P., Franco, B., Romio, L., Bleicher, F. (2009). Primary cilia of odontoblasts: Possible role in molar morphogenesis. Journal of Dental Research, 88(10), 910–915. https://doi.org/10.1177/0022034509345822
dc.relation.referencesTsumura, M., Sobhan, U., Muramatsu, T., Sato, M., Ichikawa, H., Sahara, Y., Shibukawa, Y. (2012). TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. Cell Calcium, 52(2), 124–136. https://doi.org/10.1016/j.ceca.2012.05.002
dc.relation.referencesVandewauw, I., Owsianik, G., & Voets, T. (2013). Systematic and quantitative mRNA expression analysis of TRP channel genes at the single trigeminal and dorsal root ganglion level in mouse. BMC Neuroscience, 14(21). https://doi.org/10.1186/1471- 2202-14-21
dc.relation.referencesWen, W., Que, K., Zang, C., Wen, J., Sun, G., Zhao, Z., & Li, Y. (2017). Expression and distribution of three transient receptor potential vanilloid(TRPV) channel proteins in human odontoblast-like cells. Journal Of Molecular Histology, 48(5–6), 367–377. https://doi.org/10.1007/s10735-017-9735-2
dc.relation.referencesWu, A., Bao, Y., Yu, H., Zhou, Y., & Lu, Q. (2019). Berberine Accelerates Odontoblast Differentiation by Wnt/β-Catenin Activation. Cellular Reprogramming, 21(2), 108– 114. https://doi.org/10.1089/cell.2018.0060
dc.relation.referencesYang, B.H., Piao, Z.G., Kim, Y-B., Lee, C-H., Lee ,J.K., Park, K., Kim, J.S., & Oh S.B.(2013). Activation of Vanilloid Receptor 1 (VR1) by Eugenol. Journal of Dental Research, 10(82), 781–785.
dc.relation.referencesYang, F., Xiao, X., Cheng, W., Yang, W., Yu, P., Song, Z., Zheng, J. (2015). binding and activation of the TRPV1 ion channel. Nature Chemical Biology, 12(june), 1–9. https://doi.org/10.1038/nchembio.1835
dc.relation.referencesYokose, S., & Naka, T. (2010). Lymphocyte enhancer-binding factor 1: An essential factor in odontoblastic differentiation of dental pulp cells enzymatically isolated from rat incisors. Journal of Bone and Mineral Metabolism, 28(6), 650–658. https://doi.org/10.1007/s00774-010-0185-0
dc.relation.referencesYu, C., & Abbott, P. (2007). An overview of the dental pulp: its functions and responses to injury. Australian Dental Journal, 52, S4–S6. https://doi.org/10.1111/j.1834- 7819.2007.tb00525.x
dc.relation.referencesZhang, F., Jara-oseguera, A., Chang, T., Bae, C., Hanson, S. M., & Swartz, K. J. (2017). Heat activation is intrinsic to the pore domain of TRPV1. PNAS, 317–324. https://doi.org/10.1073/pnas.1717192115
dc.relation.referencesJung, J., Shin, J. S., Lee, S., Hwang, S. W., Koo, J., Cho, H., & Oh, U. (2004). Phosphorylation of Vanilloid Receptor 1 by Ca 2 / Calmodulin- dependent Kinase II Regulates Its Vanilloid Binding. The Journal of Biological Chemistry, 279(8), 7048– 7054. https://doi.org/10.1074/jbc.M311448200
dc.relation.referencesSanz-salvador, L., Andres-borderia, A., Ferrer-montiel, A., & Planells-cases, R. (2012).Agonist- and Ca 2 -dependent Desensitization of TRPV1 Channel Targets the Receptor to Lysosomes for. The Journal of Biological Chemistry, 287(23), 19462–19471. https://doi.org/10.1074/jbc.M111.289751
dc.relation.referencesMohapatra, Durga Prasanna, & Nau, C. (2005). Regulation of Ca 2 -dependent Desensitization in the Vanilloid Receptor TRPV1 by Calcineurin and cAMP dependent Protein Kinase *. The Journal of Biological Chemistry, 280(14), 13424– 13432. https://doi.org/10.1074/jbc.M410917200
dc.relation.referencesMinisterio de Salud y Protección Social. (2014). IV Estudio Nacional de Salud BucalENSAB. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ENSAB-IVSituacion-Bucal-Actual.pdf
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.proposalOdontoblasts
dc.subject.proposalOdontoblasto
dc.subject.proposalHipersensibilidad dentinal
dc.subject.proposalToothache
dc.subject.proposalReceptor TRPV1
dc.subject.proposalTRPV1 cation channels
dc.subject.proposalReceptor TRP
dc.subject.proposalTRPV1 receptor
dc.subject.proposalTransiet receptor ponential channel
dc.type.coarhttp://purl.org/coar/resource_type/c_1843
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2


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