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Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.

dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.contributor.advisorPolanía, Jaime
dc.contributor.advisorVanegas Guerrero, Javier
dc.contributor.authorSepúlveda Correa, Alejandro
dc.date.accessioned2021-08-11T16:38:37Z
dc.date.available2021-08-11T16:38:37Z
dc.date.issued2021
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/79917
dc.descriptionilustraciones
dc.description.abstractLos manglares responden a factores bióticos y abióticos, como la salinidad variable y el estrés antropogénico (por ejemplo, la contaminación por desechos urbanos). Esos factores también pueden afectar a las poblaciones de microorganismos e incluso a la relativa abundancia de genes que intervienen en las respuestas biológicas. Esta investigación tenía por objeto identificar los genes asociados con la resistencia y la biosíntesis de los compuestos antimicrobianos en los suelos de manglares sometidos a salinidades contrastantes. El ADN total se extrajo de muestras de suelo rizosférico de tres áreas con diferentes salinidades en un manglar contaminado con aguas residuales en la desembocadura del río Ranchería (La Guajira, Colombia) para su secuenciamiento mediante Illumina HiSeq 2500. El análisis de la diversidad funcional y taxonómica reveló un dominio de 51 familias y 182 géneros de actinobacterias. Las vías de biosíntesis más comunes fueron para la estreptomicina y los monobactámicos. Fue posible asociar 43 genes relacionados con la síntesis de compuestos antimicrobianos, y la abundancia de 24 de ellos se vio considerablemente influida por la salinidad. El aumento de la concentración de sal influyó en las vías metabólicas y en la abundancia diferencial de los genes asociados con la síntesis de compuestos antimicrobianos (por ejemplo, rfbB / rffG, INO1 / ISYNA1, rfbA / rffH, sat / met3, asd, lysC, proA, aspB, fabG). Además, de los 29 genes implicados en la resistencia intrínseca a los antibióticos, 16 estaban influidos significativamente por la salinidad (por ejemplo, aacA, oleC4, oleC5, vgb, acrB / mexB / adeJ / smeE / mtrD / cmeB, bpeF, mexF). Se concluye que los mecanismos de tolerancia y adaptabilidad a las condiciones de estrés salino favorecerían la síntesis de compuestos antimicrobianos en los manglares sujetos a contaminación por aguas residuales. (Tomado de la fuente)
dc.description.abstractMangroves respond to biotic and abiotic factors, such as variable salinity and anthropogenic stress (e.g., pollution from urban waste). Such factors may also affect populations of microorganisms and even the relative abundance of genes involved in biological responses. This research aimed to identify genes associated with resistance and biosynthesis of antimicrobial compounds in mangrove soils subjected to contrasting salinities. Total DNA was extracted from rhizospheric soil samples from three areas with different salinities in a wastewater contaminated mangrove at the Ranchería River's mouth (La Guajira, Colombia) for sequencing using Illumina HiSeq 2500. Functional and taxonomic diversity analysis revealed a domain of 51 families and 182 genera of actinobacteria. The most common biosynthesis pathways were for streptomycin and monobactamics. It was possible to associate 43 genes associated with the synthesis of antimicrobial compounds, and the abundance of 24 of them was significantly influenced by salinity. The increase in salt concentration influenced the metabolic pathways and the differential abundance of genes associated with the synthesis of antimicrobial compounds (e.g., rfbB / rffG, INO1 / ISYNA1, rfbA / rffH, sat / met3, asd, lysC, proA, aspB, fabG). In addition, out of 29 genes involved in intrinsic antibiotic resistance, 16 were significantly influenced by salinity (e.g. aacA, oleC4, oleC5, vgb, acrB / mexB / adeJ / smeE / mtrD / cmeB, bpeF, mexF). It is concluded that the mechanisms of tolerance and adaptability to the conditions of saline stress would favor the synthesis of antimicrobial compounds in mangroves subject to contamination by wastewater. (Tomado de la fuente)
dc.format.extent68 páginas
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia Sede Medellin
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc500 - Ciencias naturales y matemáticas
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::634 - Huertos, frutas, silvicultura
dc.subject.otherActinobacterias
dc.titleCaracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
dc.typeTrabajo de grado - Maestría
dcterms.audienceEspecializada
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programMedellín - Ciencias Agrarias - Maestría en Bosques y Conservación Ambiental
dc.description.degreelevelMaestría
dc.description.degreenameMagister en Bosques y Conservación Ambiental
dc.description.researchareaComponente físico, biológico, químico y geológico del medio marino e hídrico continental
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.departmentDepartamento de Ciencias Forestales
dc.publisher.facultyFacultad de Ciencias Agrarias
dc.publisher.placeMedellín
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellín
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.lembAguas residuales
dc.subject.lembManglares (Ecología)
dc.subject.proposalManglares
dc.subject.proposalEcología microbiana
dc.subject.proposalMetabolitos secundarios
dc.subject.proposalResistencia antimicrobiana
dc.subject.proposalAmbientes costeros áridos
dc.subject.proposalMicrobial ecology
dc.subject.proposalSecondary metabolites
dc.subject.proposalAntibiotics resistance
dc.subject.proposalArid coastal environments
dc.title.translatedMetagenomic characterization of genes associated with the synthesis and resistance of antimicrobial compounds in mangrove soils.
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
oaire.awardtitleDiversidad funcional de microorganismos asociados al ciclaje de C, N y P en el manglar la Ranchería (La Guajira) mediante un acercamiento de metatranscriptómica
oaire.fundernameMinisterio de Ciencia, Tecnología e Innovación


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