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Evaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.

dc.rights.licenseReconocimiento 4.0 Internacional
dc.contributor.advisorSoto Sedano, Johana Carolina
dc.contributor.advisorChaparro Giraldo, Alejandro
dc.contributor.authorRodriguez Abril, Edna Yadira
dc.date.accessioned2021-10-16T15:24:25Z
dc.date.available2021-10-16T15:24:25Z
dc.date.issued2021-11-09
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/80567
dc.descriptionilustraciones, diagramas
dc.description.abstractEn esta investigación se estudiaron cuatro genotipos de soya colombiana (Brasilera 1, Brasilera 2, FNS01 y Soy SK-7), bajo un sistema de transformación genética mediada por Agrobacterium tumefaciens cepa AGL0, induciendo organogénesis directa a partir de nodo cotiledonar y realizando selección con el herbicida glifosato. Los genotipos fueron evaluados durante las fases in vitro de inducción de brote (IB), elongación de brote (EB) y enraizamiento. Además, se evaluó la etapa de endurecimiento y se determinó la eficiencia de transformación. No se encontraron diferencias significativas entre las cuatro variedades durante la fase in vitro ni durante el endurecimiento. Se obtuvieron transformantes de las cuatro variedades y la variedad Brasilera 1 presentó la mayor eficiencia de transformación con 3.7%. (Texto tomado de la fuente)
dc.description.abstractIn this research, four Colombian soybean genotypes (Brasilera 1, Brasilera 2, FNS01 y Soy SK-7) were studied under a system of genetic transformation mediated by Agrobacterium tumefaciens, strain AGL0, inducing direct organogenesis from the cotyledonary node and performing selection with the herbicide glyphosate. Genotypes were evaluated during the in vitro sprout induction (IB), sprout elongation (BE) and rooting phases. In addition, the hardening stage was evaluated, and transformation efficiency was determined. No significant differences were found between the four varieties during the in vitro phases or during hardening. Transformants of the four varieties were obtained, and Brasilera 1 presented the highest transformation efficiency at 3.7%.
dc.format.extentxviii, 55 páginas
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
dc.titleEvaluación del comportamiento in vitro de genotipos de soya durante la transformación genética mediada por Agrobacterium tumefaciens.
dc.typeTrabajo de grado - Maestría
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programMedellín - Ciencias Agrarias - Maestría en Ciencias Agrarias
dc.contributor.researchgroupIngeniería Genética de Plantas
dc.description.degreelevelMaestría
dc.description.degreenameMagister en ciencias agrarias
dc.description.researchareaGenética y fitomejoramiento
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.departmentDepartamento de Agronómicas
dc.publisher.facultyFacultad de Ciencias Agrarias
dc.publisher.placeMedellín, Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellín
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.lembSoybean
dc.subject.lembSoya
dc.subject.lembIn vitro culture
dc.subject.lembCiltivo in vitro
dc.subject.lembRegeneración in vitro
dc.subject.proposalGenotipos
dc.subject.proposalRegeneración
dc.subject.proposalTransformación
dc.subject.proposalGlifosato
dc.subject.proposalGenotype
dc.subject.proposalRegeneration
dc.subject.proposalTransformation
dc.subject.proposalGlyphosate
dc.title.translatedEvaluation of the in vitro behavior of soybean genotypes during genetic transformation mediated by Agrobacterium tumefaciens.
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