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Determinación y caracterización de las regiones de unión de PfRON4 a eritrocitos y hepatocitos humanos

dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.contributor.advisorArévalo Pinzón, Gabriela
dc.contributor.advisorPatarroyo Gutiérrez, Manuel Alfonso
dc.contributor.authorPulido Quevedo, Fredy Alexander
dc.date.accessioned2023-01-18T16:27:59Z
dc.date.available2023-01-18T16:27:59Z
dc.date.issued2023-01-17
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83010
dc.descriptionilustraciones
dc.description.abstractPlasmodium falciparum durante su ciclo de vida, expresa una amplia gama de proteínas entre las que se destaca la proteína del cuello de las roptrias 4 (PfRON4). Este es un candidato promisorio a vacuna, ya que se expresa tanto en merozoítos como esporozoítos, participa durante la formación del enlace fuerte con la célula hospedera a través del complejo RONs/AMA1 y es refractario a deleción genética. Pese a ello, aún no se conocen las regiones clave de este antígeno que interactúan con las células hospederas, siendo esta información de gran utilidad para combatir la enfermedad causada por este parásito. Por tal motivo, en este trabajo de investigación se sintetizaron 32 péptidos derivados de la región conservada de PfRON4 y se llevaron a cabo ensayos de interacción receptor-ligando para determinar la capacidad de unión de cada péptido a células hospederas, así como determinar la naturaleza del receptor y habilidad de éstos para inhibir la invasión del parásito en cultivo continuo in vitro con la cepa FCB2. Se identificaron cinco HABPs (High Activity Binding Peptides) denominados 42477, 42479, 42480, 42505 y 42513, los cuales se unieron con alta afinidad y especificidad a receptores de tipo proteico sobre la membrana de los eritrocitos. Por su parte, los péptidos 42477 y 42480 se unieron a la membrana de las células HepG2 con constantes de disociación en el rango sub-micromolar, siendo esta interacción dependiente de receptores tipo heparina y/o sulfato de condroitina. Los ensayos de inhibición de la invasión mostraron que los HABPs de PfRON4 fueron capaces de bloquear la entrada de los merozoítos a los eritrocitos hasta en un 50%. En conclusión, se encontró que las regiones de PfRON4 800-819 (42477) y 860-879 (42480) interactúan específicamente con las células hospederas y esto soporta su inclusión en el desarrollo de una vacuna multi-antígeno, multi-estadio basada en subunidades contra P. falciparum (Texto tomado de la fuente)
dc.description.abstractPlasmodium falciparum expresses a wide range of proteins during its lifecycle, among which one the most important is rhoptry neck protein 4 (PfRON4); it is a promising vaccine candidate since it is expressed in merozoites and sporozoites, participates in a strong bond formation with host cells via the RONs/AMA-1 complex and is refractory to genetic deletion. Despite this, PfRON4’s key regions interacting with host cells remain unknown; such information would be extremely useful for combating P. falciparum-related malaria. For this reason, in this research work, thirty-two PfRON4 conserved region-derived synthetic peptides were chemically synthesized, and receptor-ligand interaction/binding assays were carried out for determining the binding capacity of each peptide to host cells, the nature of their receptors and their ability to inhibit in vitro parasite invasion with the FCB2 strain. Five HABPS (High Activity Binding Peptides) named 42477, 42479, 42480, 42505 and 42513 were identified, which bound with high affinity and specificity to protein-like receptors on the erythrocyte membrane. Peptides 42477 and 42480 bound to HepG2 cells’ membrane, both of them having submicromolar range kD, the interaction being dependent on heparin and/or chondroitin sulphate proteoglycan receptors. Invasion inhibition assays showed that PfRON4 HABPs were able to block merozoite entry into erythrocytes by up to 50%. In conclusion, PfRON4 regions 800-819 (42477) and 860-879 (42480) were found to specifically interact with host cells, which supports their inclusion in the development of a subunit-based, multi-antigen, multistage anti-malarial vaccine against P. falciparum
dc.format.extentxv, 90 páginas
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc570 - Biología::572 - Bioquímica
dc.subject.ddc610 - Medicina y salud::616 - Enfermedades
dc.titleDeterminación y caracterización de las regiones de unión de PfRON4 a eritrocitos y hepatocitos humanos
dc.typeTrabajo de grado - Maestría
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programBogotá - Medicina - Maestría en Bioquímica
dc.contributor.researchgroupReceptor-Ligando
dc.description.degreelevelMaestría
dc.description.degreenameMagister en Bioquímica
dc.description.researchareaBioquímica y Biología Molecular
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.facultyFacultad de Medicina
dc.publisher.placeBogotá, Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.lembVacunas
dc.subject.lembVaccines
dc.subject.lembParásitos
dc.subject.lembParasites
dc.subject.proposalmalaria
dc.subject.proposalPlasmodium falciparum
dc.subject.proposalproteína del cuello de las roptrias 4
dc.subject.proposalpéptidos sintéticos.
dc.subject.proposalrhoptry neck protein 4
dc.subject.proposalsynthetic peptides
dc.title.translatedDetermination and characterization PfRON4's binding regions to human erythrocytes and hepatocytes
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
dcterms.audience.professionaldevelopmentInvestigadores
dc.contributor.orcidhttps://orcid.org/0000-0003-4395-3672


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