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Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado
dc.rights.license | Reconocimiento 4.0 Internacional |
dc.contributor.advisor | Restrepo Betancur, Giovanni |
dc.contributor.author | Zapata Carmona, Kelly Vanessa |
dc.date.accessioned | 2023-02-06T15:23:28Z |
dc.date.available | 2023-02-06T15:23:28Z |
dc.date.issued | 2022-11 |
dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/83312 |
dc.description | ilustraciones, diagramas |
dc.description.abstract | Durante la refrigeración, el espermatozoide porcino puede experimentar estrés oxidativo, osmótico, químico y térmico, que afectan su capacidad fecundante. La adición de antioxidantes al diluyente espermático constituye una alternativa para mitigar dichas alteraciones. El objetivo de esta investigación fue evaluar la capacidad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) sometido a refrigeración. Para ello, quince eyaculados de cinco cerdos fueron diluidos en MRA, suplementados con Silimarina, Silibinina, Silicristina y Silidianina y refrigerados cinco días a 16°C. Cada 24 h se evaluó la movilidad y cinética espermática con un CASA IVOS. A las 0 y 96 h se determinaron la funcionalidad de membrana por test hipoosmótico; la producción de EROs y la capacidad antioxidante total (ABTS-FRAP), por espectrofluorimetría; el potencial de membrana mitocondrial, la integridad estructural y la peroxidación lipídica mediante citometría de flujo, con JC-1, SYBR14/IP y Bodipy respectivamente. Se realizaron comparaciones de medias por Duncan, se calculó un índice para calidad espermática y se hicieron análisis de regresión para movilidad y cinética espermática, y para cinética de EROs. La suplementación con Silidianina (10-20 µM) incrementó MT, MP, Δ¥M y VE, y redujo la producción de EROs. Silicristina, a partir de 20 µM, disminuyó la MT, MP, Δ¥M y VE. Mientras que Silimarina redujo la producción de EROs/minuto. En definitiva, la Silimarina y sus componentes pueden incrementar la calidad espermática, reducir la generación de EROs, potenciar la CAT y modificar el Δ¥M en el semen porcino refrigerado. (Texto tomado de la fuente) |
dc.description.abstract | In refrigerated conditions, boar spermatozoa are susceptible to oxidative, osmotic, chemical and thermal stress, affecting their fertilizing capacity. The addition of antioxidants to the sperm extender is an alternative to mitigate these alterations. The objective of this research was to evaluate the antioxidant capacity of Silymarin and its components (Silidianin, Silicristin and Silibinin) and their effect on the preservation of refrigerated boar semen (Sus scrofa). For this purpose, fifteen ejaculates from five swine were diluted in MRA, supplemented with Silymarin, Silybinin, Silychristin and Silydianin and refrigerated for five days at 16°C. Sperm motility and sperm kinetics were evaluated every 24 h with a CASA IVOS. At 0 and 96 h, membrane functionality was determined by hypoosmotic test; EROs production and total antioxidant capacity (ABTS-FRAP), by spectrofluorimetry; mitochondrial membrane potential, structural integrity and lipid peroxidation by flow cytometry with JC-1, SYBR14/PI and Bodipy probes, respectively. Duncan mean comparisons were performed, an index for sperm quality was calculated and regression analyses were performed for sperm motility and sperm kinetics, and for EROs kinetics. Silydianin supplementation (10-20 µM) increased MT, MP, Δ¥M and VE, and reduced EROs production. Silychristin, from 20 µM, decreased MT, MP, Δ¥M and VE. While Silymarin reduced EROs/minute production. In conclusion, Silymarin and its components can improve sperm quality, reduce EROs generation, enhance CAT and modify Δ¥M in refrigerated boar semen. |
dc.format.extent | xvi, 107 páginas |
dc.format.mimetype | application/pdf |
dc.language.iso | spa |
dc.publisher | Universidad Nacional de Colombia |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.subject.ddc | 590 - Animales::599 - Mamíferos |
dc.title | Actividad antioxidante de la Silimarina y sus componentes (Silidianina, Silicristina y Silibinina) y su efecto sobre la conservación de semen porcino (Sus scrofa) refrigerado |
dc.type | Trabajo de grado - Maestría |
dc.type.driver | info:eu-repo/semantics/masterThesis |
dc.type.version | info:eu-repo/semantics/acceptedVersion |
dc.publisher.program | Medellín - Ciencias - Maestría en Ciencias - Biotecnología |
dc.contributor.researchgroup | Grupo de Investigación en Biotecnología Animal (Giba) |
dc.description.degreelevel | Maestría |
dc.description.degreename | Magíster en Ciencias - Biotecnología |
dc.description.researcharea | Reproducción Animal |
dc.identifier.instname | Universidad Nacional de Colombia |
dc.identifier.reponame | Repositorio Institucional Universidad Nacional de Colombia |
dc.identifier.repourl | https://repositorio.unal.edu.co/ |
dc.publisher.faculty | Facultad de Ciencias |
dc.publisher.place | Medellín, Colombia |
dc.publisher.branch | Universidad Nacional de Colombia - Sede Medellín |
dc.relation.indexed | RedCol |
dc.relation.indexed | LaReferencia |
dc.relation.indexed | Agrovoc |
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dc.rights.accessrights | info:eu-repo/semantics/openAccess |
dc.subject.lemb | Conservación del semen |
dc.subject.lemb | Semen conservation |
dc.subject.proposal | Calidad seminal |
dc.subject.proposal | Estado redox |
dc.subject.proposal | Flavonolignanos |
dc.subject.proposal | Refrigeración |
dc.subject.proposal | Silimarina |
dc.subject.proposal | Semen porcino |
dc.subject.proposal | Flavonolignans |
dc.subject.proposal | Porcine semen |
dc.subject.proposal | Redox status |
dc.subject.proposal | Refrigeration |
dc.subject.proposal | Seminal quality |
dc.subject.proposal | Silymarin |
dc.title.translated | Antioxidant activity of silymarin and its components (silydianin, silychristin and silybinin) and their effect on the preservation of refrigerated boar semen (Sus scrofa) |
dc.type.coar | http://purl.org/coar/resource_type/c_bdcc |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa |
dc.type.content | Text |
dc.type.redcol | http://purl.org/redcol/resource_type/TM |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 |
dcterms.audience.professionaldevelopment | Estudiantes |
dcterms.audience.professionaldevelopment | Investigadores |
dcterms.audience.professionaldevelopment | Maestros |
dc.description.curriculararea | Área Curricular Biotecnología |
dc.contributor.orcid | Zapata Carmona, Kelly Vanessa [0000-0002-3559-5783] |
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