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Ensamblaje del genoma de Leuconostoc mesenteroides IBUN 91.2.98. por medio de herramientas bioinformáticas

dc.rights.licenseReconocimiento 4.0 Internacional
dc.contributor.advisorOspina Sánchez, Sonia Amparo
dc.contributor.authorNúñez Campos, César Augusto
dc.date.accessioned2023-04-24T13:54:41Z
dc.date.available2023-04-24T13:54:41Z
dc.date.issued2022-10-07
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83760
dc.descriptionilustraciones
dc.description.abstractA pesar de la importancia del dextrano en varias aplicaciones industriales y la necesidad de masificar su producción, no se tiene muy documentado el mecanismo de expresión y regulación de las dextransacarasas en cepas productoras como el Leuconostoc mesenteroides cepa IBUN 91.2.98. El desarrollo de métodos de secuenciación de segunda generación y el uso de herramientas bioinformáticas permitirán secuenciar, ensamblar y evaluar genomas completos a un costo relativamente bajo guiados por un genoma de referencia de L. mesenteroides subsp. mesenteroides ATCC 8293, para ayudar al proceso de ensamblaje. La secuenciación produjo un total de 1.47 Gb de datos crudos, que después del trimming y control de calidad generaron 1,40 Gb (7.78 X de profundidad) que se usaron para el ensamblaje. Se obtuvo un ensamblaje para el Leuconostoc mesenteroides cepa IBUN 91.2.98 de una longitud de 2,064 Mpb. La longitud del ensamblaje represento el 85% del tamaño estimado del genoma de referencia de L. mesenteroides subsp. mesenteroides ATCC 8293. (Texto tomado de la fuente)
dc.description.abstractDespite the importance of dextran in various industrial applications and the need to massify your production, the mechanism of expression and regulation of dextransucrases in producer strains such as Leuconostoc mesenteroides strain IBUN 91.2.98 has not been well documented. The development of second-generation sequencing methods and the use of bioinformatic tools will make it possible to sequence, assemble, and evaluate complete of genomes at relatively low cost guided by a reference genome of L. mesenteroides subsp. mesenteroides ATCC 8293, to help the assembly process. Sequencing produced a total of 1.47 Gb of raw data, which after trimming and quality control were generated 1.40 Gb (7.78 X deep) which was used for assembly. An assembly for Leuconostoc mesenteroides strain IBUN 91.2.98 with a length of 2,064 Mpb was obtained. The assembly length represented 85% of the estimated size of the reference genome of L. mesenteroides subsp. mesenteroides ATCC 8293.
dc.format.extent59 páginas
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ddc000 - Ciencias de la computación, información y obras generales
dc.subject.ddc500 - Ciencias naturales y matemáticas
dc.subject.ddc520 - Astronomía y ciencias afines
dc.titleEnsamblaje del genoma de Leuconostoc mesenteroides IBUN 91.2.98. por medio de herramientas bioinformáticas
dc.typeTrabajo de grado - Maestría
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Química
dc.contributor.researchgroupBiopolímeros y Biofuncionales
dc.description.degreelevelMaestría
dc.description.degreenameMagíster en Ciencias - Química
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.facultyFacultad de Ciencias
dc.publisher.placeBogotá,Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.decsGenoma
dc.subject.decsGenome
dc.subject.decsTecnología de bajo costo
dc.subject.decsLow Cost Technology
dc.subject.proposalreads
dc.subject.proposalcontigs
dc.subject.proposalensamblaje
dc.subject.proposaltecnologías de secuenciación
dc.subject.proposaltraducción
dc.title.translatedAssembly of the genome of Leuconostoc mesenteroides IBUN 91.2.98. through bioinformatics tools
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
dcterms.audience.professionaldevelopmentInvestigadores
dc.contributor.orcidCésar Augusto Núñez Campos [0000000152190836]
dc.contributor.cvlacNúñez Campos, César Augusto


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