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Caracterización morfológica y molecular de bacterias asociadas a Capsicum spp. en el Valle del Cauca-Colombia

dc.rights.licenseAtribución-NoComercial 4.0 Internacional
dc.contributor.advisorGarcía Dávila, Mario Augusto
dc.contributor.advisorGómez López, Eyder Daniel
dc.contributor.authorRivera Calderón, Angela Liliana
dc.date.accessioned2023-08-09T21:21:51Z
dc.date.available2023-08-09T21:21:51Z
dc.date.issued2023-07-25
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84514
dc.descriptionIlustraciones, fotografías, tablas, gráficas
dc.description.abstractEl cultivo de Capsicum en el Valle del Cauca en Colombia tiene un alto potencial agrícola e industrial afectado por diferentes factores bióticos y abióticos; las bacterias pueden ocasionar pérdidas entre 10 y 20 % y, en condiciones ambientales favorables para su desarrollo, hasta del 100 %. Para abordar esta problemática el objetivo de este estudio fue caracterizar bacterias fitopatógenas y aquellas con potencial biocontrolador de las anteriores. Se recolectó material vegetal afectado en 17 zonas del departamento, de las cuales se obtuvieron 69 aislamientos: 8 aislamientos fueron fitopatógenos Gram negativos y 4 con potencial biocontrolador Gram positivos. Se realizaron las siguientes caracterizaciones: cultural, morfológica, bioquímica (pruebas API 20NE), patogénica y molecular (región del 16S rADN, GyrB y GyrA), y se evaluó el potencial biocontrolador in vitro. Los aislamientos fitopatógenos identificados fueron: tres especies del género Pseudomonas (Pseudomonas asiatica, P. straminea, P. synxantha); uno asociado al subgrupo de Pseudomonas straminea; dos asociados a las enterobacterias: Kosakonia cowanii y Pantoea agglomerans; y dos de Ralstonia solanacearum. Los primeros seis se consideran el primer reporte para Colombia y de los últimos dos no hay reporte en la literatura colombiana asociados a Capsicum spp. La caracterización patogénica se evaluó en los cultivares Habanero, Tabasco y Cayenne, encontrándose que los aislamientos asociados a enterobacterias y Ralstonia solanacearum fueron los más virulentos. Los aislamientos con potencial biocontrolador se caracterizaron como Bacillus amyloliquefaciens; de estos, el aislamiento 43 presentó los mayores halos de inhibición sobre las fitopatógenas. Durante las pruebas in vitro se evaluó la importancia de que estos aislamientos se sembraran 48 horas antes de las fitopatógenas. Como resultado, se determinaron seis nuevas especies de fitopatógenas no relacionadas anteriormente al cultivo de Capsicum en Colombia. Esto constituye un aporte significativo al conocimiento. Se encontró que los aislamientos obtenidos de Bacillus amyloliquefaciens tienen potencial biocontrolador. (Texto tomado de la fuente)
dc.description.abstractThe Capsicum crop in the department of Valle del Cauca in Colombia has a high agricultural and industrial potential that is affected by different biotic and abiotic factors. Bacteria can cause losses between 10 and 20 % and under favorable environmental conditions for their development up to 100 %. The objective of this study was established to characterize pathogenic and those with bio-control potential bacteria. Affected plant material was collected in 17 sites of the department, from which 69 isolates were obtained: 8 isolates were Gram negative phytopathogenic and 4 Gram positive isolates with biocontrol potential were selected. The following characterizations were carried out: Cultural, morphological, biochemical (API 20NE test), pathogenic and molecular (16S rDNA region, GyrB and GyrA); and the in vitro biocontrol potential was evaluated The phytopathogenic isolates identified were three species of Pseudomonas genus: (Pseudomonas asiatica, P. straminea, P. syxantha), one associated with the subgroup of Pseudomonas straminea; two associated with enterobacteria: Kosakonia cowanii and Pantoea agglomerans; and two from Ralstonia solanacearum. The first six isolates are considered the first report for Colombia and of the last two; there are no reports in the Colombian literature associated with Capsicum spp. The pathogenic characterization was evaluated in the cultivars Habanero, Tabasco and Cayenne, finding that the isolates associated with enterobacteriaceae and Ralstonia solanacearum were the most virulent. Isolates with biocontroller potential were characterized as Bacillus amyloliquefaciens; Of these, isolation 43 presented the largest halos of inhibition on phytopathogens During the in vitro tests, the importance of these isolates being seeded 48 hours before the phytopathogens was evaluated. As a result, six new species of plant pathogens not previously related to the cultivation of Capsicum in Colombia were determined. This constitutes a significant contribution to knowledge. It was found that the isolates obtained from Bacillus amyloliquefaciens have biocontrol potential.
dc.description.sponsorshipConvocatoria 727 de 2015 Doctorados Nacionales Colciencias
dc.format.extentxxii, 111 páginas + anexos
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
dc.titleCaracterización morfológica y molecular de bacterias asociadas a Capsicum spp. en el Valle del Cauca-Colombia
dc.typeTrabajo de grado - Doctorado
dc.type.driverinfo:eu-repo/semantics/doctoralThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programPalmira - Ciencias Agropecuarias - Doctorado en Ciencias Agrarias
dc.contributor.researchgroupProtección Vegetal Para El Mejoramiento de la Productividad
dc.coverage.regionValle del Cauca, Colombia
dc.description.degreelevelDoctorado
dc.description.degreenameDoctor en Ciencias Agrarias
dc.description.methodsLa metodología desarrollada durante esta investigación fue elaborada con base en los objetivos planteados. Se realizaron colectas de tejido sano y enfermo en Cultivos de Capsicum spp en el Valle del Cauca. Las muestras fueron procesadas en los laboratorios de Microbiología Agrícola y Diagnóstico Vegetal. A partir de estas muestras se realizó aislamientos de las bacterias hasta obtenerlas en cultivo puro. Los aislamientos se caracterizaron cultural, morfológica, patogénica, bioquímica y molecularmente. Además se estableció el efecto biorregulador de bacterias no patogénicas en el desarrollo de las bacterias fitopatógenas in vitro
dc.description.researchareaProtección de Cultivos
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.departmentDoctorado en Ciencias Agrarias
dc.publisher.facultyFacultad de Ciencias Agropecuarias
dc.publisher.placePalmira, Valle del Cauca, Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Palmira
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dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccess
dc.subject.agrovocColombia
dc.subject.agrovocValle del Cauca
dc.subject.agrovocCaracterización molecular
dc.subject.agrovocMolecular characterization
dc.subject.agrovocBacterias patógenas
dc.subject.agrovocPathogenic bacteria
dc.subject.agrovocInfectious Agent
dc.subject.agrovocAgente infeccioso
dc.subject.agrovocEnfermedades de las plantas
dc.subject.agrovocPlant diseases
dc.subject.agrovocEnfermedades bacterianas
dc.subject.agrovocBacterial diseases
dc.subject.proposalFitobacteria
dc.subject.proposalFitobacteriología
dc.subject.proposalFitopatología
dc.subject.proposalBiorregulador
dc.subject.proposalCapsicum spp.
dc.subject.proposalPlant bacteriology
dc.subject.proposalPhytopathogen
dc.subject.proposalPhytopathology
dc.subject.proposalBiocontrol
dc.subject.proposalPlant bacteria
dc.title.translatedMorphological and molecular characterization of plant bacteria associated to Capsicum spp. in Valle del Cauca - Colombia
dc.type.coarhttp://purl.org/coar/resource_type/c_db06
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/TD
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
oaire.awardtitleProyecto 39584. Caracterización de fitobacterias asociadas a Capsicum spp en el Valle del Cauca
oaire.awardtitleProyecto 39897. Caracterización morfológica y molecular de bacterias asociadas a ají y pimentón Capsicum spp. en el Valle del Cauca
oaire.fundernameColciencias
oaire.fundernameUniversidad Nacional de Colombia
dcterms.audience.professionaldevelopmentEstudiantes
dcterms.audience.professionaldevelopmentInvestigadores
dc.description.curricularareaCiencias Agropecuarias.Sede Palmira
dc.contributor.orcidhttps://orcid.org/0000-0003-1831-481X
dc.contributor.orcidAngela Liliana Rivera-Calderón
dc.contributor.cvlacRivera Calderón, Angela Liliana
dc.contributor.cvlachttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000331465
dc.contributor.researchgatehttps://www.researchgate.net/profile/Angela-Rivera-2
dc.contributor.researchgateAngela Rivera
dc.contributor.googlescholarAngela Liliana Rivera Calderón
dc.contributor.googlescholarhttps://scholar.google.com/citations?user=-dDzVswAAAAJ&hl=es&oi=ao


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