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Caracterización morfológica y molecular de aislamientos de Ceratocystis sp. provenientes de cultivos de cacao en Colombia

dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.contributor.advisorGonzález Almario, Adriana
dc.contributor.authorCarrero Gutiérrez, Martha Liliana
dc.date.accessioned2023-12-06T16:33:12Z
dc.date.available2023-12-06T16:33:12Z
dc.date.issued2023-12-06
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85043
dc.descriptionilustraciones, diagramas, fotogtrafías
dc.description.abstractDurante los últimos años, los problemas fitosanitarios del cacao en Colombia han sido la principal causa de la reducción de la producción del cultivo, con pérdidas que pueden llegar al 100% de la producción por manejos inadecuados. El mal de machete es una de las enfermedades que afecta el cultivo de cacao en el país y actualmente se ha reportado a Ceratocystis fimbriata como el agente causal de la enfermedad. Sin embargo, aún existen incompatibilidades entre autores que contradicen la relación causal de este patógeno con la enfermedad. En Colombia, se conoce muy poco sobre la importancia de esta enfermedad, la identidad y aspectos taxonómicos del patógeno. Nuestro estudio tuvo como objetivo caracterizar morfológica y molecularmente aislamientos de Ceratocystis sp. obtenidos de material leñoso sintomático y muestras de suelo, provenientes de diferentes zonas productoras de cacao en Colombia. Para este propósito, se evaluaron diferentes caracteres morfológicos macro y microscópicos asociados al crecimiento de las colonias y a la medición de rasgos morfométricos. La identificación molecular a nivel de especie y la caracterización filogenética de los aislamientos se llevaron a cabo a través de un análisis multilocus, incluyendo como marcadores moleculares los genes β-Tubulina (βT-1), región del gen de la subunidad de proteína de unión a nucleótidos de guanina (MS204), segunda subunidad mayor de la RNA polimerasa (RPBII) y 60S proteína ribosomal L37 (FG1093). Nuestros resultados revelaron variaciones en el desarrollo de las colonias sin un patrón específico. A nivel microscópico, el aislamiento NCF16 mostró características morfológicas diferenciales a lo reportado previamente para la especie C. fimbriata. Los análisis moleculares y filogenéticos permitieron clasificar todos los aislamientos utilizados en este estudio como Ceratocystis cacaofunesta, confirmando que el agente causal del mal de machete en cacao en Colombia es la especie C. cacaofunesta; para la cual, no se tenía certeza sobre su identidad. Este estudio reveló la variabilidad morfológica y genética que presenta Ceratocystis cacaofunesta en este cultivo, y se confirma que dicha variabilidad, no está relacionada con el lugar ni con el tejido o sustrato de origen. Sin embargo, destacamos la importancia de evaluar estos rasgos en una población mayor de aislamientos (Texto tomado de la fuente)
dc.description.abstractThe main thread of cocoa crop production in Colombia are the phytosanitary problems. Nowadays, the production losses that can be up to the 100% due to the inadequate diseases management. The mal de machete is one of the limitans diseases that affect the cocoa crop in the country and currently, Ceratocystis fimbriata has been reported as the causal agent of disease; however, there are still discrepancies among authors that contradict the causal relationship with the disease. In Colombia, very little is known the importance of this disease, the identity, and taxonomic aspects of the pathogen. Our study aimed characterizing morphologically and molecularly isolates of Ceratocystis sp. obtained from symptomatic woody material and soil samples from different cocoa producing areas in Colombia. For this purpose, different macro, and microscopic morphological characters associated with colony growth and morphometric traits were evaluated. Ceratocystis species identification and phylogenetic characterization were performed through a multilocus analysis including β-tubulin gene (βT-1), guanine nucleotide-binding protein subunit gene region (MS204), second largest subunit of RNA polymerase II (RPBII), and 60S ribosomal protein L37 gene (FG1093) as taxonomic markers. Our results revealed variations in the colonies development without any specific pattern. At microscopic level, the NCF16 isolate showed morphological differences compared to with that previously reported for the species C. fimbriata. The phylogenetic analyses allowed classify isolations including in this study as C. cacaofunesta, confirming that the cause of the mal de machete disease of cacao in Colombia is the C. cacaofunesta species, of which there was uncertainty about its identity. This study revealed the morphological and genetic variability present in the Ceratocystis genus in Colombia, we did not find correlation between the variability and variables like location, tissue, or substrate of origin. However, we highlight the importance of assessing these traits in a bigger population of Ceratocystis species
dc.format.extentxx, 82 páginas
dc.format.mimetypeapplication/pdf
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
dc.subject.ddc570 - Biología::576 - Genética y evolución
dc.subject.ddc000 - Ciencias de la computación, información y obras generales::006 - Métodos especiales de computación
dc.titleCaracterización morfológica y molecular de aislamientos de Ceratocystis sp. provenientes de cultivos de cacao en Colombia
dc.typeTrabajo de grado - Maestría
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.publisher.programBogotá - Ciencias Agrarias - Maestría en Ciencias Agrarias
dc.contributor.educationalvalidatorGonzález Almario, Carolina
dc.contributor.researchgroupMejoramiento Genético Vegetal, Uso y Aprovechamiento de la Agrobiodiversidad (MGVA) - AGROSAVIA
dc.coverage.countryColombia
dc.description.degreelevelMaestría
dc.description.degreenameMagister en Ciencias Agrarías-Fitopatología
dc.description.researchareaFitopatología
dc.identifier.instnameUniversidad Nacional de Colombia
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourlhttps://repositorio.unal.edu.co/
dc.publisher.facultyFacultad de Ciencias Agrarias
dc.publisher.placeBogotá, Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
dc.relation.referencesAbdi, H., & Williams, L. J. (2010). Principal component analysis. In Wiley Interdisciplinary Reviews: Computational Statistics, Vol. 2, Issue 4, pp. 433–459. https://doi.org/10.1002/wics.101
dc.relation.referencesAlvares Vivas, A. (2011). Identificación, conservación y conformación de un banco de hongos filamentosos aislados previamente de los páramos de cruz verde y guasca. Tesis de Maestría, Pontificia Universidad Javeriana-Sede Bogotá
dc.relation.referencesArbeláez Giraldo, E. (1957). La llaga macana del tronco del cacao. Acta Agronómica, 7(1).71- 103. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/49070
dc.relation.referencesAyres, L. V. (2014). Diversidade genética de Ceratocystis fimbriata em Eucalyptus spp. no Sul da Bahia. Tesis de Maestría, Universidad Federal de Viçosa (UFV)
dc.relation.referencesBailey, B. A., & Meinhardt, L. W. (2016). Cacao diseases a history of old enemies and new encounters. Springer International Publishing Switzerland. doi 10.1007/978-3-319-24789- 2_1
dc.relation.referencesBarba Donoso, C. (1961). Estudio morfológico y pruebas de patogenicidad de varias cepas de Ceratocystis fimbriata ell. & halst. Tesis de Maestría, Instituto Interamericano de Ciencias Agrícolas de la OEA
dc.relation.referencesBaker, C. J., Harrington, T. C., Krauss, U., & Alfenas, A. C. (2003). Ecology and population biology genetic variability and host specialization in the Latin American clade of Ceratocystis fimbriata. Phytopathology, 93:1274-1284
dc.relation.referencesBarnes, I., Fourie, A., Wingfield, M. J., Harrington, T. C., McNew, D. L., Sugiyama, L. S., Luiz, B. C., Heller, W. P., & Keith, L. M. (2018). New Ceratocystis species associated with rapid death of metrosideros polymorpha in Hawaii. Persoonia: Molecular Phylogeny and Evolution of Fungi, 154–181. https://doi.org/10.3767/persoonia.2018.40.07
dc.relation.referencesBarnes, I., Gaur, A., Burgess, T., Roux, J., Wingfield, B. D., & Wingfield, M. J. (2001). Microsatellite markers reflect intra-specific relationships between isolates of the vascular wilt pathogen Ceratocystis fimbriata. Molecular Plant Pathology, 2(6), 319–325. https://doi.org/10.1046/j.1464-6722.2001.00080.x
dc.relation.referencesBarnes, I., Roux, J., Wingfield, B. D., Dudzinski, M. J., Old, K. M., & Wingfield, M. J. (2003). Ceratocystis pirilliformis, a new species from Eucalyptus nitens in Australia. Micología, 95(5), 865–871. https://doi.org/10.1080/15572536.2004.11833046
dc.relation.referencesBLAST® Command Line Applications User Manual. https://scicomp.ethz.ch/public/manual/BLAST/BLAST.pdf
dc.relation.referencesBejarano, C. A. P., Kafuri, L. A., & García, J. M. P. (2021). Identification of Phytophthora spp. isolates obtained from cocoa crops in Antioquia, Colombia. Acta agronómica, 70(1). https://doi.org/10.15446/acag.v70n1.70619
dc.relation.referencesBrawner, J., Japarudin, Y., Lapammu, M., Rauf, R., Boden, D., & Wingfield, M. J. (2015). Evaluating the inheritance of Ceratocystis acaciivora symptom expression in a diverse Acacia mangium breeding population. Southern Forests, 77(1), 83–90. https://doi.org/10.2989/20702620.2015.1007412
dc.relation.referencesCapella-Gutiérrez, S., Silla-Martínez, J. M., & Gabaldón, T. (2009). trimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics, 25(15), 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
dc.relation.referencesCastrejón-Antonio, J. E., Montesinos-Matías, R., Acevedo-Reyes, N., Tamez-Guerra, P., Ayala-Zermeño, M. Á., Berlanga-Padi-lla, A. M. y Arredondo-Bernal, H. C. (2017) Especies de Xyleborus (Coleoptera: Curculionidae: Scolytinae) asociados a huertos de aguacate en Colima, México. Acta Zoológica Mexicana (n.s.), 33(1), 146-150
dc.relation.referencesCiencia y tecnología al servicio del sector cacaotero. (2016). http://www.fhia.org.hn/descargas/proyecto_procacao/infocacao/InfoCacao_No11_Dic_2016.pdf
dc.relation.referencesCordero Vega, M. J. (2017). Caracterización morfológica, patogénica y molecular de aislamientos de Ceratocystis spp. provenientes de seis zonas cafetaleras de Costa Rica. Tesis para optar por el grado de profesional de Ingeniería Agronómica, Universidad de Costa Rica
dc.relation.referencesCrous, P. W., Wingfield, M. J., Guarro, J., Hernández-Restrepo, M., Sutton, D. A., Acharya, K., Barber, P. A., Boekhout, T., Dimitrov, R. A., Dueñas, M., Dutta, A. K., Gené, J., Gouliamova, D. E., Groenewald, M., Lombard, L., Morozova, O. V., Sarkar, J., Th Smith, M., Stchigel, A. M. Groenewald, J. Z. (2015). Fungal Planet description sheets: 320–370. Persoonia: Molecular Phylogeny and Evolution of Fungi, 34, 167–266. https://doi.org/10.3767/003158515X688433
dc.relation.referencesde Beer, Z. W., Duong, T. A., Barnes, I., Wingfield, B. D., & Wingfield, M. J. (2014). Redefining Ceratocystis and allied genera. Studies in Mycology, 79(1), 187–219. https://doi.org/10.1016/j.simyco.2014.10.001. De Moraes Russo, C. A., & Selvatti, A. P. (2018). Bootstrap and rogue identification tests for phylogenetic analyses. Molecular Biology and Evolution, 35(9), 2327–2333. https://doi.org/10.1093/molbev/msy118
dc.relation.referencesde Mendiburu F (2023). _agricolae: Statistical Procedures for Agricultural Research_. R. package version 1.3-6. https://cran.r-project.org/web/packages/agricolae/index.html
dc.relation.referencesDe Moraes Russo, C. A., & Selvatti, A. P. (2018). Bootstrap and rogue identification tests for phylogenetic analyses. Molecular Biology and Evolution, 35(9), 2327–2333. https://doi.org/10.1093/molbev/msy118
dc.relation.referencesDelgado, R., & Suárez, C. (2003). Genetic dissection of late blight resistance in cultivated diploid Solanum species and modern potato varieties of Ecuador View project. https://www.researchgate.net/publication/234058127
dc.relation.referencesDos Santos Nunes, L. (2015). Resistência de Mangueira “ubá” (Mangifera indica) à murcha-de Ceratocystis (Ceratocystis fimbriata). Tesis de Maestría, Universidad Federal de Viçosa (UFV)
dc.relation.referencesEngelbrecht, C. J. B. (2004). Host specialization, intersterility, and taxonomy of populations of Ceratocystis fimbriata from sweet potato, sycamore, and cacao. http://lib.dr.iastate.edu/rtd
dc.relation.referencesEngelbrecht, C. J. B., & Harrington, T. C. (2005). Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato, cacao, and sycamore. Mycologia, 97(1), 57–69. https://doi.org/10.3852/mycologia.97.1.57
dc.relation.referencesEngelbrecht, C. J., Harrington, T. C., & Alfenas, A. (2007). Ceratocystis wilt of cacao - A disease of increasing importance. Phytopathology, 97(12), 1648–1649. https://doi.org/10.1094/PHYTO-97-12-1648
dc.relation.referencesFEDECACAO. (2022). Año cacaotero 2020-2021, el de mayor producción de cacao en la historia de Colombia. https://www.fedecacao.com.co/post/a%C3%B1o-cacaotero-2020-2021-el-de-mayor-producci%C3%B3n-de-cacao-en-la-historia-de-colombia
dc.relation.referencesFergus, C. L. (1956). The Influence of Actidione on Wood-Staining Fungi. Mycologia, 48(4), 468– 472. https://doi.org/10.1080/00275514.1956.12024558
dc.relation.referencesFerreira, E. M., Harrington, T. C., Thorpe, D. J., & Alfenas, A. C. (2010). Genetic diversity and interfertility among highly differentiated populations of Ceratocystis fimbriata in Brazil. Plant Pathology, 59(4), 721–735. doi.org/10.1111/j.1365-3059.2010.02275.x
dc.relation.referencesFirmino, A. C. (2011). Caracterização de isolados de Ceratocystis sp., avaliação de resistência clonal de eucalipto e sensibilidade deste fungo a diferentes fungicidas. Tesis Doctoral, Universidade Estadual Paulista, Julio de Mesquita Filho
dc.relation.referencesFischer, M. C., Rellstab, C., Leuzinger, M., Roumet, M., Gugerli, F., Shimizu, K. K., Holderegger, R., & Widmer, A. (2017). Estimating genomic diversity and population differentiation - an empirical comparison of microsatellite and SNP variation in Arabidopsis halleri. BMC Genomics, 18(1). https://doi.org/10.1186/s12864-016-3459-7
dc.relation.referencesFourie, A., Wingfield, M. J., Wingfield, B. D., & Barnes, I. (2014). Molecular markers delimit cryptic species in Ceratocystis sensu stricto. Mycological Progress, 14(1). https://doi.org/10.1007/s11557-014-1020-0
dc.relation.referencesGaviria, M. A., & Cano, L. E., (2013). Taxonomía molecular de aislamientos de Fusarium obtenidos a partir de muestras clínicas. Tesis Doctoral, Universidad Nacional de Colombia, sede Medellín
dc.relation.referencesGriffith, G. W., & Shaw, D. S. (1998). Polymorphisms in Phytophthora infestans: Four mitochondrial haplotypes are detected after PCR Amplification of DNA from pure cultures or from host lesions. In Applied and Environmental Microbiology (Vol. 64, Issue 10). https://journals.asm.org/journal/aem
dc.relation.referencesHarrington, T. C. (1981). Cycloheximide sensitivity as a taxonomic character in Ceratocystis. Mycologia, 73(6), 1123. https://doi.org/10.2307/3759682
dc.relation.referencesHarrington, T. C. (2013). Ceratocystis Diseases. 11. Lowa State University, Ames, Iowa, USA
dc.relation.referencesHarrington, T. C., Kazmin, M. R., Al-Sadi, A. M., Ismail, S. I. (2014). Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto. Mycologia,106(2), 224–242. https://doi10.3852/13- 189
dc.relation.referencesHall, T. (2004). BioEdit Versión 7.0.0. Manual. https://studylib.net/doc/14572292/bioedit-version-7.0.0
dc.relation.referencesHeath, R. N., Wingfield, M. J., Wingfield, B. D., Meke, G., Roux, Heath R N, Wingfield M J, Wingfield B D, & Mbaga A. (2009). Ceratocystis species on Acacia mearnsii and Eucalyptus spp. in eastern and southern Africa including six new species
dc.relation.referencesHeberth, D., & Ortíz, R. (2011). Caracterización etiológica de la Roña de la gulupa (Passiflora edulis Sims.) en la región del Sumapaz (Cundinamarca). Tesis de Maestría, Universidad Nacional de Colombia sede Bogotá
dc.relation.referencesHolland, L. A., Lawrence, D. P., Nouri, M. T., Travadon, R., Harrington, T. C., & Trouillas, F. P. (2019). Taxonomic revision and multi-locus phylogeny of the North American clade of Ceratocystis. Fungal Systematics and Evolution, 3, 135–156. https://doi.org/10.3114/fuse.2019.03.07
dc.relation.referencesHolland, L. A., Trouillas, F. P., Nouri, M. T., Lawrence, D. P., Crespo, M., Doll, D. A., Duncan, R. A., Holtz, B. A., Culumber, C. M., Yaghmour, M. A., Niederholzer, F. J. A., Lightle, D. M., Jarvis-Shean, K. S., Gordon, P. E., & Fichtner, E. J. (2021). Fungal pathogens associated with canker diseases of almond in California. Plant Disease, 105(2). https://doi.org/10.1094/PDIS-10-19-2128-RE
dc.relation.referencesHuang, C. H., Lee, F. L., & Tai, C. J. (2009). The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 95(2), 135–142. https://doi.org/10.1007/s10482-008-9296-1
dc.relation.referencesInstituto Colombiano Agropecuario-ICA. (2012). Manejo fitosanitario del cultivo del cacao. Medidas para la temporada invernal. https://www.ica.gov.co/getattachment/c01fa43b-cf48-497a-aa7f-51e6da3f7e96/-
dc.relation.referencesJabeen, K., & Asad, S. (2017). Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death). Jabeen and Asad, 148(5), 148–154. http://www.innspub.net
dc.relation.referencesJaimes, Y., & Aranzazu, F. (2010). Manejo de las enfermedades del cacao (Theobroma cacao L) en Colombia, con énfasis en Monillia (Moniliophthora roreri). Corporación colombiana de Investigación Agropecuaria - Corpoica. https://doi.org/10.21930/978-958-740-034-2
dc.relation.referencesJohnson, J. A., Harrington, T. C., & Engelbrecht, C. J. B. (2005). Phylogeny and taxonomy of the North American clade of the Ceratocystis fimbriata complex
dc.relation.referencesJollife, I. T., & Cadima, J. (2016). Principal component analysis: A review and recent developments. In Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences (Vol. 374, Issue 2065). Royal Society of London. https://doi.org/10.1098/rsta.2015.0202
dc.relation.referencesKaiser, H. F. (1961). A note on guttman’s lower bound for the number of common factors. British Journal of Statistical Psychology, 14(1), 1–2. https://doi.org/10.1111/j.2044- 8317.1961.tb00061.x
dc.relation.referencesKanzi, A. M., Trollip, C., Wingfield, M. J., Barnes, I., Van Der Nest, M. A., & Wingfield, B. D. (2020). Phylogenomic incongruence in Ceratocystis: A clue to speciation? BMC Genomics, 21(1). https://doi.org/10.1186/s12864-020-6772-0
dc.relation.referencesKatoh, K., Rozewicki, J., & Yamada, K. D. (2018). MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 20(4), 1160–1166. https://doi.org/10.1093/bib/bbx108
dc.relation.referencesKück, P., & Longo, G. C. (2014). FASconCAT-G: Extensive functions for multiple sequence alignment preparations concerning phylogenetic studies. Frontiers in Zoology, 11(1). https://doi.org/10.1186/s12983-014-0081-x
dc.relation.referencesKassambara, A. and Mundt, F. (2020). Factoextra: Extract and Visualize the Results of Multivariate Data Analyses. R Package Version 1.0.7. https://CRAN.R-project.org/package=factoextra
dc.relation.referencesKatoh, K., Rozewicki, J., & Yamada, K. D. (2018). MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics, 20(4), 1160–1166. https://doi.org/10.1093/bib/bbx108
dc.relation.referencesKück, P., & Longo, G. C. (2014). FASconCAT-G: Extensive functions for multiple sequence alignment preparations concerning phylogenetic studies. Frontiers in Zoology, 11(1). https://doi.org/10.1186/s12983-014-0081-x
dc.relation.referencesLê, S., Josse, J., Rennes, A., & Husson, F. (2008). FactoMineR: An R Package for Multivariate Analysis. In JSS Journal of Statistical Software (Vol. 25). http://www.jstatsoft.org/
dc.relation.referencesLiu, F. F., Barnes, I., Roux, J., Wingfield, M. J., & Chen, S. F. (2018). Molecular phylogenetics and microsatellite analysis reveal a new pathogenic Ceratocystis species in the Asian-Australian clade. Plant Pathology, 67(5), 1097–1113. https://doi.org/10.1111/ppa.12820
dc.relation.referencesLiu, Y. J., Whelen, S., & Hall, B. D. (1999). Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerse II subunit. Molecular Biology and Evolution, 16(12), 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
dc.relation.referencesLurá, María C., Benitez, Joel D; Jáuregui, Soledad; Gonzáles, Ana M. Evaluación de diferentes técnicas de extracción del ADN de hongos filamentosos. Revista FABICIB V 7: 37-44
dc.relation.referencesMagalhães, D. M. A., Luz, E. D. M. N., Lopes, U. V., Niella, A. R. R., & Damaceno, V. O. (2016). Leaf disc method for screening Ceratocystis wilt resistance in cacao. Tropical Plant Pathology, 41(3), 155–161. https://doi.org/10.1007/s40858-016-0081-9
dc.relation.referencesMarelli, J. P., Guest, D. I., Bailey, B. A., Evans, H. C., Brown, J. K., Junaid, M., Barreto, R. W., Lisboa, D. O., & Puig, A. S. (2019). Chocolate under threat from old and new cacao diseases. In Phytopathology (Vol. 109, Issue 8, pp. 1331–1343). American Phytopathological Society. https://doi.org/10.1094/PHYTO-12-18-0477-RVW
dc.relation.referencesMarin, M., Castro, B., Gaitan, A., Preisig, O., Wingfield, B. D., & Wingfield, M. J. (2003). Relationships of Ceratocystis fimbriata isolates from Colombian coffee-growing regions based on molecular data and pathogenicity. Journal of Phytopathology, 151(7–8), 395– 405. https://doi.org/10.1046/j.1439-0434.2003.00738.x
dc.relation.referencesMarin, M., & Wingfield, M. J. (2006). A review of Ceratocystis sensu stricto with special reference to the species complexes C. coerulescens and C. fimbriata Article in Revista Facultad Nacional de Agronomía Medellín. Biological control of Eucalypt pests (BiCEP) View project Global movement of eucalypts pathogens; implications for biodiversity and biosecurity View project. https://www.researchgate.net/publication/235651222
dc.relation.referencesMayorga, S. (2020). Relaciones filogenéticas en el complejo de especies Pedaliodes Butler, 1867 (Lepidoptera: Nymphalidae) basadas en el marcador molecular COI. Tesis para optar el grado de Biólogo, Pontificia Universidad Javeriana
dc.relation.referencesModels_. R package version 3.1-162. https://cran.r-project.org/web/packages/nlme/index.html
dc.relation.referencesMota-Gutierrez, J., Botta, C., Ferrocino, I., Giordano, M., Bertolino, M., Dolci, P., Cannoni, M., & Cocolin, L. (2018). Dynamics and Biodiversity of Bacterial and Yeast Communities during Fermentation of Cocoa Beans. Applied and Environmental Microbiology, 84(19). https://doi.org/10.1128/AEM.01164-18
dc.relation.referencesO’, K., 1~, D., Nirenberg, H. I., Aoki, T., & Cigelnik, E. (2000). A Multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. In Mycoscience (Vol. 41)
dc.relation.referencesObaid, A., Adawi, A., Deadman, M., & Wingfield, B. D. (2007). Phytopathology of horticultural crops of oman View project fungi associated with wood-boring insects: diversity, pathogenicity and risks related to timber trade and climate change View project Marelize van Wyk National Institute for Communicable Diseases. https://www.researchgate.net/publication/215735977
dc.relation.referencesO’Donnell, K., Nirenberg, H. I., Aoki, T., & Cigelnik, E. (2000). A Multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. In Mycoscience (Vol. 41)
dc.relation.referencesO’Donnell, K., & Cigelnik, E. (1997). Two Divergent Intragenomic rDNA ITS2 Types within a Monophyletic Lineage of the Fungus Fusarium are Nonorthologous. Molecular phylogenetics and evolution, Vol. 7, No. 1, pp. 103–116
dc.relation.referencesOliveira, L. S. S., Guimarães, L. M. S., Ferreira, M. A., Nunes, A. S., Pimenta, L. V. A., & Alfenas, A. C. (2015). Aggressiveness, cultural characteristics, and genetic variation of Ceratocystis fimbriata on Eucalyptus spp. Forest Pathology, 45(6), 505–514. https://doi.org/10.1111/efp.12200
dc.relation.referencesOliveira, L. S. S., Harrington, T. C., Ferreira, M. A., Damacena, M. B., Al-Sadi, A. M., Al-Mahmooli, I. H. S., & Alfenas, A. C. (2015). Species or genotypes? Reassessment of four recently described species of the Ceratocystis wilt pathogen, Ceratocystis fimbriata, on Mangifera indica. Phytopathology, 105(9), 1229–1244. https://doi.org/10.1094/PHYTO-03-15-0065-R
dc.relation.referencesPaladines-Rezabala, A., Moreira-Morrillo, A. A., Mieles, A. E., & Garcés-Fiallos, F. R. (2022). Advances in understanding of the interaction between Ceratocystis cacaofunesta and Xyleborus ferrugineus (Coleoptera: Curculionidae: Scolytinae) on cocoa trees. In Scientia Agropecuaria (Vol. 13, Issue 1, pp. 43–52). Universidad Nacional de Trujillo. https://doi.org/10.17268/sci.agropecu.2022.004
dc.relation.referencesPicó, M., & Esteras, C. (s.f.). Marcadores moleculares basados en PCR: Marcadores SSR o STR. Microsatélites. Universidad Politécnica de Valencia
dc.relation.referencesPinheiro, J; Bates D; DebRoy, S; Sarkar, D. R. C. T. (2022). nlme: Linear and Nonlinear Mixed Effects Models. R package version 3.1-155. https://cran.rproject.org/package=nlme
dc.relation.referencesProcter, J. B., Carstairs, G. M., Soares, B., Mourão, K., Ofoegbu, T. C., Barton, D., Lui, L., Menard, A., Sherstnev, N., Roldan-Martinez, D., Duce, S., Martin, D. M. A., & Barton, G. J. (2021). Alignment of Biological Sequences with Jalview. In Methods in Molecular Biology (Vol. 2231, pp. 203–224). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1036-7_13
dc.relation.referencesPromoción de Turismo, Inversión y Exportanciones – Proexport. (2012). Cacao colombiano fino y de aroma. https://gestionparticipativa.pe.iica.int/getattachment/8010f039-fabc-465d-8c48-5a914c3da9da/Cacao-Colombiano-fino-y-de-aroma.aspx
dc.relation.referencesQuang Minh, B., Lanfear, R., Ly-Trong Jana Trifinopoulos, N., Schrempf, D., & Schmidt, H. A. (2022). IQ-TREE version 2.2.0: Tutorials and Manual Phylogenomic software by maximum likelihood. http://www.iqtree.org
dc.relation.referencesQuinlan, A. R. (2014). BEDTools: The Swiss-Army tool for genome feature analysis. Current Protocols in Bioinformatics, 2014, 11.12.1-11.12.34. https://doi.org/10.1002/0471250953.bi1112s47
dc.relation.referencesR Core Team. (2022). R: a Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Version 4.1.3. https://www.R-project.org/
dc.relation.referencesRambaut, A. (2018). Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. FigTree v 1.4.4. http://tree.bio.ed.ac.uk/software/figtree/
dc.relation.referencesReyna Ávila, R. G. (2015). Estudios epidemiológicos del marchitamiento por Ceratocystis en Eucalypto. Tesis de Maestría, Universidad de la República
dc.relation.referencesRobles, J., & Alméciga, J. (2013). Caracterización e identificación molecular de hongos de suelo aislados de los páramos de Guasca y Cruz Verde, Cundinamarca-Colombia. Tesis para optar el grado de Microbiólogo Industrial. Pontificia Universidad Javeriana
dc.relation.referencesReyes, B. M. D., Fonseca, P. L. C., Heming, N. M., Conceição, L. B. de A., Nascimento, K. T. de S., Gramacho, K. P., Arevalo-Gardini, E., Pirovani, C. P., & Aguiar, E. R. G. R. (2023). Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1053562
dc.relation.referencesRodas, C. A., Van Wyk, M., Wingfield, B. D., Wingfield, M. J., Van Wyk, J., & Wingfield, M. (2008). Ceratocystis neglecta sp. nov., infecting Eucalyptus trees in Colombia
dc.relation.referencesRodríguez-Polanco, E., Morales, J. G., Muñoz-Agudelo, M., Segura, J. D., & Carrero, M. L. (2020). Morphological, molecular, and pathogenic characterization of phytophthora palmivora isolates causing black pod rot of cacao in colombia. Spanish Journal of Agricultural Research, 18(2), 1–15. https://doi.org/10.5424/sjar/2020182-15147
dc.relation.referencesRojas, G. (2008). Estudio de aislamientos del hongo fitopatógeno del café Ceratocystis fimbriata. Tesis para optar el grado de Ingeniería en Biotecnología, Instituto tecnológico de Costa Rica
dc.relation.referencesRonquist, F., Huelsenbeck, J. P., Teslenko, M., Zhang, C., & Nylander, J. A. A. (2020). MrBayes version 3.2 Manual: Tutorials and Model Summaries
dc.relation.referencesSantini, A., Capretti, P., & Santini, A. (2000). Analysis of the Italian population of Ceratocystis fimbriata f.sp. platani using RAPD and minisatellite markers. In Plant Pathology (Vol. 49). https://www.researchgate.net/publication/30068960
dc.relation.referencesSantos, P. L., Firmino, A. C., Tozze, H. J., de Barros, S. A., & Furtado, E. L. (2011). “In vitro” Ceratocystis sp. behavior under differents temperatures, culture medium and pH. Revista Eletrônica de Educação e Ciência – REEC. 1(1), 07-17. ISSN 2237-3462
dc.relation.referencesSuárez Contreras, L. Y., & Peñaranda Figueredo, F. A. (2021). Identificación molecular de hongos filamentosos y su potencial biotecnológico. Biotecnología En El Sector Agropecuario y Agroindustrial, 20(1), 194–206. https://doi.org/10.18684/rbsaa.v20.n1.2022.1914
dc.relation.referencesSuwandi, S., Irsan, C., Hamidson, H., Umayah, A., & Asriyani, K. D. (2021). Identification and characterization of Ceratocystis fimbriata causing lethal wilt on the lansium tree in Indonesia. Plant Pathology Journal, 37(2). https://doi.org/10.5423/PPJ.OA.08.2020.0147
dc.relation.referencesTarigan, M., Roux, J., Van Wyk, M., Tjahjono, B., & Wingfield, M. J. (2011). A new wilt and die-back disease of Acacia mangium associated with Ceratocystis manginecans and C. acaciivora sp. nov. in Indonesia. South African Journal of Botany, 77(2), 292–304. https://doi.org/10.1016/j.sajb.2010.08.006
dc.relation.referencesTen Hoopen, G. M., Deberdt, P., Mbenoun, M., & Cilas, C. (2012). Modelling cacao pod growth: Implications for disease control. Annals of Applied Biology, 160(3), 260–272. https://doi.org/10.1111/j.1744-7348.2012.00539.x
dc.relation.referencesTumura1, K. G., De, C., Edson, P. ;, & Furtado2, L. (2012). Murcha por Ceratocystis em eucalipto: avaliação de resistência e análise epidemiológica. In Summa Phytopathol (Issue 1)
dc.relation.referencesValdetaro, D. C. O. F., Oliveira, L. S. S., Guimarães, L. M. S., Harrington, T. C., Ferreira, M. A., Freitas, R. G., & Alfenas, A. C. (2015). Genetic variation, morphology, and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil. Tropical Plant Pathology, 40(3), 184–192. https://doi.org/10.1007/s40858-015-0036-6
dc.relation.referencesVan Wyk, M., Wingfield, B. D., Marin, M., & Wingfield, M. J. (2010). New Ceratocystis species infecting coffee, cacao, citrus, and native trees in Colombia. Fungal Diversity, 40, 103–117. https://doi.org/10.1007/s13225-009-0005-9
dc.relation.referencesWalker, D. M., Castlebury, L. A., Rossman, A. Y., & White, J. F. (2012). New molecular markers for fungal phylogenetics: Two genes for species-level systematics in the Sordariomycetes (Ascomycota). Molecular Phylogenetics and Evolution, 64(3), 500–512. https://doi.org/10.1016/j.ympev.2012.05.005
dc.relation.referencesWard, J. H. (1963). Hierarchical Grouping to Optimize an Objective Function. Journal of the American Statistical Association, 58(301), 236–244. https://doi.org/10.1080/01621459.1963.10500845
dc.relation.referencesWebster A N, R. K., & Butler, D. E. E. (1967). A morphological and biological concept of tile species Ceratocystis fimbriata. In Canadian Journal of Botany (Vol. 45). www.nrcresearchpress.com
dc.relation.referencesWingfield, M. J., & Wingfield, B. D. (2010). Ceratocystis species on Acacia mearnsii and Eucalyptus spp. in eastern and southern Africa including six new species the fungal diversity associated with Stipagrostis species of fairy circles in the Namib desert View project Global movement of eucalypts pathogens: implications for biodiversity and biosecurity View project. https://www.researchgate.net/publication/228440532
dc.relation.referencesWitthuhn, R. C., Wingfield, B. D., Wingfield, M. J., and Harrington, T. C. (1999). PCR-based identification and phylogeny of species of Ceratocystis sensu stricto. Mycol, 103(6): 74.3- 74.9
dc.relation.referencesWyk, M. Van, Wingfield, B. D., Al-Adawi, A. O., Rossetto, C. J., Ito, M. F., & Wingfield, M. J. (2011). Two new Ceratocystis species associated with mango disease in Brazil. Mycotaxon, 117, 381–404. https://doi.org/10.5248/117.381
dc.relation.referencesXu, K., Li, J., Yang, X., Zhang, R., Li, X., Xie, M., & Huang, Q. (2020). Postharvest rot on carrot caused by Ceratocystis fimbriata and Chalaropsis thielavioides (≡ Thielaviopsis thielavioides) in China. In Journal of General Plant Pathology (Vol. 86, Issue 4, pp. 322–325). Springer. https://doi.org/10.1007/s10327-020-00919-1
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.subject.decsCell Nucleus Shape
dc.subject.decsForma del nucleo celular
dc.subject.lembCacao
dc.subject.proposalC. cacaofunesta
dc.subject.proposalTheobroma cacao L
dc.subject.proposalMal de machete
dc.subject.proposalCaracterización
dc.subject.proposalMorfología
dc.subject.proposalFilogenia
dc.subject.proposalC. cacaofunesta
dc.subject.proposalTheobroma cacao L
dc.subject.proposalMal de machete
dc.subject.proposalCharacterizations
dc.subject.proposalMorphology
dc.subject.proposalPhylogeny
dc.title.translatedMorphological and molecular characterization of Ceratocystis sp. isolates from cocoa crops in Colombia
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
oaire.awardtitleCaracterización morfológica y molecular de aislamientos de Ceratocystis sp. provenientes de cultivos de cacao en Colombia
oaire.fundernameCorporación Colombiana de Investigación Agropecuaria- AGROSAVIA
dcterms.audience.professionaldevelopmentEstudiantes
dcterms.audience.professionaldevelopmentInvestigadores
dcterms.audience.professionaldevelopmentMaestros
dc.contributor.orcidCarrero Gutiérrez, Martha [0000000195100730]
dc.contributor.googlescholarCarrero Gutiérrez, Martha [https://scholar.google.es/citations?hl=es&user=BKoXwpUAAAAJ&view_op=list_works&sortby=pubdate]


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