Potencial probiótico de bacterias ácido lácticas aisladas de leche materna en Antioquia

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dc.contributor.advisorMárquez Fernández, María Elena
dc.contributor.authorBolívar Parra, Laura
dc.contributor.cvlacBolívar Parra, Laura [0001452822]
dc.contributor.educationalvalidatorÚsuga Monroy, Cristina
dc.contributor.educationalvalidatorMontoya Campuzano, Olga Inés
dc.contributor.educationalvalidatorRuiz Villadiego, Orlando Simón
dc.contributor.orcidBolívar Parra, Laura [0000000273402479]
dc.contributor.orcidÚsuga-Monroy, Cristina [0000000161012994]
dc.contributor.orcidRuiz Villadiego, Orlando Simon [0000000325552867]
dc.contributor.orcidMontoya Campuzano, Olga Inés [0000000248200381]
dc.contributor.researchgroupProbióticos: Prospección Funcional y Metabolitos
dc.coverage.regionAntioquia (Colombia)
dc.date.accessioned2026-02-18T13:19:57Z
dc.date.available2026-02-18T13:19:57Z
dc.date.issued2025
dc.descriptionIlustraciones
dc.description.abstractLa leche materna (LM) es una matriz alimentaria de alto valor nutricional que contiene bacterias ácido lácticas (BAL) en su microbiota, algunas de ellas con potencial probiótico y benéficas para la salud materno-infantil. En Colombia son escasos los estudios sobre el tema, este es el primero que combina análisis moleculares, bioquímicos y microbiológicos de LM de 20 madres lactantes del departamento de Antioquia. Se identificaron y caracterizaron 151 cepas BAL, usando secuenciación del gen 16S rRNA y pruebas fenotípicas, encontrando los géneros Streptococcus (50.3%), Pediococcus (23.2%), Enterococcus (19.2%), Limosilactobacillus (6.6%) y Ligilactobacillus (0.7%). Este constituye el tercer reporte de Pediococcus en LM, lo que resalta su posible relevancia en contextos geográficos específicos. De las 151 cepas, 20 fueron evaluadas para determinar su potencial probiótico, destacando cuatro cepas: Ligilactobacillus salivarius 1Nnn, Limosilactobacillus fermentum 1Qu, Pediococcus acidilactici 2Fcc y Pediococcus pentosaceus 2Kz2, como candidatas con potencial probiótico, a tres se les confirmó su clasificación taxonómica por secuenciación genómica. Las principales propiedades probióticas observadas incluyeron susceptibilidad a antibióticos, resistencia al estrés gastrointestinal (pH y sales biliares), capacidad de adhesión y antagonismo frente a Escherichia coli (ATCC 525292), Staphylococcus aureus (ATTC 29213), Listeria monocytogenes (WDCM 00019) y Salmonella enterica subsp. enterica serovar Typhimurium (ATTC 14028). Estos hallazgos aportan conocimiento nuevo sobre el potencial probiótico de las BAL de LM en Antioquia, caracterizada por su diversidad multicultural y gastronómica e incentiva el desarrollo tecnológico de productos probióticos con beneficios en la salud humana. Se recomienda realizar estudios in vivo para validar su funcionalidad y seguridad. (Texto tomado de la fuente)spa
dc.description.abstractHuman milk (HM) is a food matrix of high nutritional value that contains lactic acid bacteria (LAB) in its microbiota, some of which have probiotic potential and are beneficial for maternal and infant health. In Colombia, studies on this topic are scarce; this study is the first to combine molecular, biochemical, and microbiological analyses of HM from 20 lactating mothers in the department of Antioquia. A total of 151 LAB strains were identified and characterized using 16S rRNA gene sequencing and phenotypic tests, corresponding to the genera Streptococcus (50.3%), Pediococcus (23.2%), Enterococcus (19.2%), Limosilactobacillus (6.6%), and Ligilactobacillus (0.7%). This is the third report of Pediococcus in HM, highlighting its possible relevance in specific geographic contexts. Of the 151 strains, 20 were evaluated for probiotic potential. Four strains—Ligilactobacillus salivarius 1Nnn, Limosilactobacillus fermentum 1Qu, Pediococcus acidilactici 2Fcc, and Pediococcus pentosaceus 2Kz2—were identified as promising candidates, and three of them were taxonomically confirmed by whole-genome sequencing. The main probiotic properties included antibiotic susceptibility, resistance to gastrointestinal stress (pH and bile salts), adhesion capacity, and antagonistic activity against Escherichia coli (ATCC 525292), Staphylococcus aureus (ATCC 29213), Listeria monocytogenes (WDCM 00019), and Salmonella enterica subsp. enterica serovar Typhimurium (ATCC 14028). These findings provide new evidence on the probiotic potential of LAB from HM in Antioquia, a region characterized by cultural and dietary diversity, and support the development of probiotic products with benefits for human health. Further in vivo studies are recommended to confirm their functionality and safety.eng
dc.description.curricularareaBiotecnología.Sede Medellín
dc.description.degreelevelDoctorado
dc.description.degreenameDoctor en Biotecnología
dc.description.notesMención meritoria por parte de los jurados evaluadoresspa
dc.description.researchareaMicrobiota
dc.description.researchareaProbióticos
dc.description.researchareaBioprospección
dc.format.extent1 recurso en línea (157 páginas)
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/89589
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellín
dc.publisher.facultyFacultad de Ciencias
dc.publisher.placeMedellín, Colombia
dc.publisher.programMedellín - Ciencias - Doctorado en Biotecnología
dc.relation.references1. Lyons KE, Ryan CA, Dempsey EM, Ross RP, Stanton C. Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health. Nutrients. 2020 Apr 9;12(4):1039.
dc.relation.references2. Duale A, Singh P, Al Khodor S. Breast Milk: A Meal Worth Having. Vol. 8, Frontiers in Nutrition. Frontiers Media S.A.; 2022.
dc.relation.references3. Zimmermann P, Curtis N. Breast milk microbiota: A complex microbiome with multiple impacts and conditioning factors. Journal of Infection. W.B. Saunders Ltd; 2020.
dc.relation.references4. Notarbartolo V, Giuffrè M, Montante C, Corsello G, Carta M. Composition of Human Breast Milk Microbiota and Its Role in Children’s Health. Pediatr Gastroenterol Hepatol Nutr. 2022;25(3):194.
dc.relation.references5. Martín R, Olivares M, Marín ML, Fernández L, Xaus J, Rodríguez JM. Probiotic Potential of 3 Lactobacilli Strains Isolated From Breast Milk. Journal of Human Lactation [Internet]. 2005 Feb 25;21(1):8–17. Available from: http://journals.sagepub.com/doi/10.1177/0890334404272393
dc.relation.references6. Martín R, Jiménez E, Olivares M, Marín ML, Fernández L, Xaus J, et al. Lactobacillus salivarius CECT 5713, a potential probiotic strain isolated from infant feces and breast milk of a mother–child pair. Int J Food Microbiol. 2006 Oct;112(1):35–43.
dc.relation.references7. Arboleya S, Ruas-Madiedo P, Margolles A, Solís G, Salminen S, de los Reyes-Gavilán CG, et al. Characterization and in vitro properties of potentially probiotic Bifidobacterium strains isolated from breast-milk. Int J Food Microbiol [Internet]. 2011 Sep;149(1):28–36. Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.036
dc.relation.references8. Zacarías MF, Binetti A, Laco M, Reinheimer J, Vinderola G. Preliminary technological and potential probiotic characterisation of bifidobacteria isolated from breast milk for use in dairy products. Int Dairy J. 2011 Aug;21(8):548–55.
dc.relation.references9. Kozak K, Charbonneau D, Sanozky-Dawes R, Klaenhammer T. Characterization of bacterial isolates from the microbiota of mothers’ breast milk and their infants. Gut Microbes [Internet]. 2015 Nov 2;6(6):341–51. Available from: https://www.tandfonline.com/doi/full/10.1080/19490976.2015.1103425
dc.relation.references10. Jiang M, Zhang F, Wan C, Xiong Y, Shah NP, Wei H, et al. Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk. J Dairy Sci [Internet]. 2016 Mar;99(3):1736–46. Available from: http://dx.doi.org/10.3168/jds.2015-10434
dc.relation.references12. Damaceno QS, Souza JP, Nicoli JR, Paula RL, Assis GB, Figueiredo HC, et al. Evaluation of Potential Probiotics Isolated from Human Milk and Colostrum. Probiotics Antimicrob Proteins [Internet]. 2017 Dec 3;9(4):371–9. Available from: http://link.springer.com/10.1007/s12602-017-9270-1
dc.relation.references12. Damaceno QS, Souza JP, Nicoli JR, Paula RL, Assis GB, Figueiredo HC, et al. Evaluation of Potential Probiotics Isolated from Human Milk and Colostrum. Probiotics Antimicrob Proteins [Internet]. 2017 Dec 3;9(4):371–9. Available from: http://link.springer.com/10.1007/s12602-017-9270-1
dc.relation.references13. Jamyuang C, Phoonlapdacha P, Chongviriyaphan N, Chanput W, Nitisinprasert S, Nakphaichit M. Characterization and probiotic properties of Lactobacilli from human breast milk. 3 Biotech [Internet]. 2019 Nov 12;9(11):398. Available from: https://doi.org/10.1007/s13205-019-1926-y
dc.relation.references14. Kang W, Pan L, Peng C, Dong L, Cao S, Cheng H, et al. Isolation and characterization of lactic acid bacteria from human milk. J Dairy Sci. 2020 Nov 1;103(11):9980–91.
dc.relation.references15. Liu W, Chen M, Duo L, Wang J, Guo S, Sun H, et al. Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum. J Dairy Sci. 2020 May;103(5):4013–25.
dc.relation.references17. Oddi S, Binetti A, Burns P, Cuatrin A, Reinheimer J, Salminen S, et al. Occurrence of bacteria with technological and probiotic potential in Argentinian human breast-milk. Benef Microbes. 2020 Nov 15;11(7):685–702.
dc.relation.references17. Oddi S, Binetti A, Burns P, Cuatrin A, Reinheimer J, Salminen S, et al. Occurrence of bacteria with technological and probiotic potential in Argentinian human breast-milk. Benef Microbes. 2020 Nov 15;11(7):685–702.
dc.relation.references18. Luz C, Calpe J, Manuel Quiles J, Torrijos R, Vento M, Gormaz M, et al. Probiotic characterization of Lactobacillus strains isolated from breast milk and employment for the elaboration of a fermented milk product. J Funct Foods. 2021 Sep;84:104599.
dc.relation.references19. D’Alessandro M, Parolin C, Patrignani S, Sottile G, Antonazzo P, Vitali B, et al. Human Breast Milk: A Source of Potential Probiotic Candidates. Microorganisms. 2022 Jun 23;10(7):1279.
dc.relation.references20. Javed GA, Arshad N, Munir A, Khan SY, Rasheed S, Hussain I. Signature probiotic and pharmacological attributes of lactic acid bacteria isolated from human breast milk. Int Dairy J. 2022 Apr;127:105297.
dc.relation.references21. Olivares M, Díaz-Ropero MP, Sierra S, Lara-Villoslada F, Fonollá J, Navas M, et al. Oral intake of Lactobacillus fermentum CECT5716 enhances the effects of influenza vaccination. Nutrition. 2007 Mar;23(3):254–60.
dc.relation.references22. Maldonado J, Cañabate F, Sempere L, Vela F, Sánchez AR, Narbona E, et al. Human Milk Probiotic Lactobacillus fermentum CECT5716 Reduces the Incidence of Gastrointestinal and Upper Respiratory Tract Infections in Infants. J Pediatr Gastroenterol Nutr. 2012 Jan;54(1):55–61.
dc.relation.references23. Gutierrez-Castrellon P, Lopez-Velazquez G, Diaz-Garcia L, Jimenez-Gutierrez C, Mancilla-Ramirez J, Estevez-Jimenez J, et al. Diarrhea in Preschool Children and Lactobacillus reuteri: A Randomized Controlled Trial. Pediatrics. 2014 Apr 1;133(4):e904–9.
dc.relation.references24. Oddi S, Huber P, Rocha Faria Duque AL, Vinderola G, Sivieri K. Breast-milk derived potential probiotics as strategy for the management of childhood obesity. Food Research International. 2020 Nov;137:109673.
dc.relation.references25. Pang B, Jin H, Liao N, Li J, Jiang C, Shao D, et al. Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. Food Funct. 2021;12(11):5171–86.
dc.relation.references26. Zimmermann P, Curtis N. Breast milk microbiota: A review of the factors that influence composition. Journal of Infection [Internet]. 2020 Jul;81(1):17–47. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0163445320300633
dc.relation.references27. Correa YN, Roldán-Pérez S, Montoya OI, Moreno PA, Castillejo NP, Velásquez- Restrepo A, et al. Caracterización de la microbiota de la leche materna de donantes y las heces de sus lactantes residentes en Antioquia, Colombia. Revista de la Facultad de Ciencias. 2023 Jan 1;12(1):6–23.
dc.relation.references28. Londoño-Sierra DC, Mesa V, Guzmán NC, Bolívar Parra L, Montoya-Campuzano OI, Restrepo-Mesa SL. Maternal Diet May Modulate Breast Milk Microbiota—A Case Study in a Group of Colombian Women. Microorganisms. 2023;11(7).
dc.relation.references29. Selma-Royo M, Calvo Lerma J, Cortés-Macías E, Collado MC. Human milk microbiome: From actual knowledge to future perspective. Semin Perinatol. 2021 Oct;45(6):151450.
dc.relation.references30. Selma-Royo M, Calvo-Lerma J, Bäuerl C, Esteban-Torres M, Cabrera-Rubio R, Collado MC. Human milk microbiota: what did we learn in the last 20 years? Microbiome Research Reports [Internet]. 2022;1(3):19. Available from: https://oaepublish.com/mrr/article/view/4896
dc.relation.references31. Roe AL, Boyte ME, Elkins CA, Goldman VS, Heimbach J, Madden E, et al. Considerations for determining safety of probiotics: A USP perspective. Regulatory Toxicology and Pharmacology. 2022 Dec;136:105266.
dc.relation.references32. Merenstein D, Pot B, Leyer G, Ouwehand AC, Preidis GA, Elkins CA, et al. Emerging issues in probiotic safety: 2023 perspectives. Gut Microbes. 2023 Dec 31;15(1).
dc.relation.references33. Food and Agriculture Organization. Probiotics in food. Health and nutritional properties and guidelines for evaluation. FAO and WH. Food and nutrition paper. Rome; 2006.
dc.relation.references34. de Melo Pereira GV, de Oliveira Coelho B, Magalhães Júnior AI, Thomaz-Soccol V, Soccol CR. How to select a probiotic? A review and update of methods and criteria. Biotechnol Adv [Internet]. 2018;36(8):2060–76. Available from: https://doi.org/10.1016/j.biotechadv.2018.09.003
dc.relation.references35. Riaz Rajoka MS, Zhao H, Mehwish HM, Li N, Lu Y, Lian Z, et al. Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk. Food Research International. 2019 Sep;123:286–97.
dc.relation.references36. Xu H, Hiraishi K, Kurahara LH, Nakano-Narusawa Y, Li X, Hu Y, et al. Inhibitory Effects of Breast Milk-Derived Lactobacillus rhamnosus Probio-M9 on Colitis-Associated Carcinogenesis by Restoration of the Gut Microbiota in a Mouse Model. Nutrients. 2021 Mar 30;13(4):1143.
dc.relation.references37. Adumuah NN, Quarshie JT, Danwonno H, Aikins AR, Ametefe EN. Exploring Anti-Breast Cancer Effects of Live Pediococcus acidilactici and Its Cell-Free Supernatant Isolated from Human Breast Milk. Int J Breast Cancer. 2024 Jan 27;2024:1–9.
dc.relation.references38. Jiménez E, Fernández L, Maldonado A, Martín R, Olivares M, Xaus J, et al. Oral Administration of Lactobacillus Strains Isolated from Breast Milk as an Alternative for the Treatment of Infectious Mastitis during Lactation. Appl Environ Microbiol. 2008 Aug;74(15):4650–5.
dc.relation.references39. Arroyo R, Martín V, Maldonado A, Jiménez E, Fernández L, Rodríguez JM. Treatment of Infectious Mastitis during Lactation: Antibiotics versus Oral Administration of Lactobacilli Isolated from Breast Milk. Clinical Infectious Diseases. 2010 Jun 15;50(12):1551–8.
dc.relation.references40. Francavilla R, Lionetti E, Castellaneta S, Ciruzzi F, Indrio F, Masciale A, et al. Randomised clinical trial: Lactobacillus reuteri DSM 17938 vs. placebo in children with acute diarrhoea ‐ a double‐blind study. Aliment Pharmacol Ther. 2012 Aug 11;36(4):363–9.
dc.relation.references41. Dinleyici EC, Vandenplas Y. Lactobacillus reuteri DSM 17938 effectively reduces the duration of acute diarrhoea in hospitalised children. Acta Paediatr. 2014 Jul 24;103(7).
dc.relation.references42. Dinleyici EC, Dalgic N, Guven S, Metin O, Yasa O, Kurugol Z, et al. Lactobacillus reuteri DSM 17938 shortens acute infectious diarrhea in a pediatric outpatient setting. J Pediatr (Rio J). 2015 Jul;91(4):392–6.
dc.relation.references43. Martin C, Ling PR, Blackburn G. Review of Infant Feeding: Key Features of Breast Milk and Infant Formula. Nutrients. 2016 May 11;8(5):279.
dc.relation.references44. Li M, Chen J, Shen X, Abdlla R, Liu L, Yue X, et al. Metabolomics-based comparative study of breast colostrum and mature breast milk. Food Chem. 2022 Aug;384:132491.
dc.relation.references45. Organización Mundial de la Salud. who.int. 2018 [cited 2020 Mar 26]. Alimentación del lactante y del niño pequeño. Available from: https://www.who.int/es/news-room/fact-sheets/detail/infant-and-young-child-feeding
dc.relation.references46. Caba-Flores MD, Ramos-Ligonio A, Camacho-Morales A, Martínez-Valenzuela C, Viveros-Contreras R, Caba M. Breast Milk and the Importance of Chrononutrition. Front Nutr. 2022 May 12;9.
dc.relation.references47. Czosnykowska-Łukacka M, Królak-Olejnik B, Orczyk-Pawiłowicz M. Breast Milk Macronutrient Components in Prolonged Lactation. Nutrients. 2018 Dec 3;10(12):1893.
dc.relation.references48. Brockway M (Merilee), Daniel AI, Reyes SM, Gauglitz JM, Granger M, McDermid JM, et al. Human Milk Bioactive Components and Child Growth and Body Composition in the First 2 Years: A Systematic Review. Advances in Nutrition. 2024 Jan;15(1):100127.
dc.relation.references49. Yi D, Kim S. Human Breast Milk Composition and Function in Human Health: From Nutritional Components to Microbiome and MicroRNAs. Nutrients. 2021 Sep 2;13(9):3094.
dc.relation.references50. Kelishadi R, Hadi B, Iranpour R, Khosravi-Darani K, Mirmoghtadaee P, Farajian S, et al. A study on lipid content and fatty acid of breast milk and its association with mother’s diet composition. Journal of Research in Medical Sciences. 2012;17(9):824–7.
dc.relation.references51. Ramiro-Cortijo D, Singh P, Liu Y, Medina-Morales E, Yakah W, Freedman SD, et al. Breast Milk Lipids and Fatty Acids in Regulating Neonatal Intestinal Development and Protecting against Intestinal Injury. Nutrients. 2020 Feb 19;12(2):534.
dc.relation.references52. Hundshammer C, Minge O. In Love with Shaping You—Influential Factors on the Breast Milk Content of Human Milk Oligosaccharides and Their Decisive Roles for Neonatal Development. Nutrients. 2020 Nov 20;12(11):3568.
dc.relation.references53. Cheng L, Akkerman R, Kong C, Walvoort MTC, de Vos P. More than sugar in the milk: human milk oligosaccharides as essential bioactive molecules in breast milk and current insight in beneficial effects. Crit Rev Food Sci Nutr. 2021 Apr 12;61(7):1184–200.
dc.relation.references54. Ni Y, Chen Q, Cai J, Xiao L, Zhang J. Three lactation-related hormones: Regulation of hypothalamus-pituitary axis and function on lactation. Mol Cell Endocrinol. 2021 Jan;520:111084.
dc.relation.references55. Organización Mundial de la Salud. who.int. 2020. Metas Globales 2025.
dc.relation.references56. World Health Organization & United Nations Children’s Fund (‎UNICEF). Estrategia mundial para la alimentacion del lactante y del nino pequeño. [Internet]. Organizacion Mundial de la Salud; 2003 [cited 2024 Jan 19]. 30 p. Available from: https://iris.who.int/handle/10665/42695
dc.relation.references57. World Health Organization. WHO recommendations on postnatal care of the mother and newborn. World Health Organization: Geneva, Switzerland [Internet]. 2014 [cited 2025 Feb 7]; Available from: https://apps.who.int/iris/bitstream/handle/10665/97603/?sequence=1
dc.relation.references58. Eidelman AI, Szilagyi G. Patterns of bacterial colonization of human milk. Obstetrics and gynecology [Internet]. 1979 May;53(5):550—552. Available from: http://europepmc.org/abstract/MED/440665
dc.relation.references59. Larson E, Zuill R, Zier V, Berg B. Storage of Human Breast Milk. Infection Control. 1984 Mar 2;5(3):127–30.
dc.relation.references60. Osterman KL, Rahm VA. Lactation Mastitis: Bacterial Cultivation of Breast Milk, Symptoms, Treatment, and Outcome. Journal of Human Lactation [Internet]. 2000 Nov 1 [cited 2023 Dec 18];16(4):297–302. Available from: https://journals.sagepub.com/doi/10.1177/089033440001600405
dc.relation.references61. Jones CA. Maternal transmission of infectious pathogens in breast milk. J Paediatr Child Health [Internet]. 2001 Dec 1 [cited 2023 Dec 18];37(6):576–82. Available from: https://onlinelibrary.wiley.com/doi/full/10.1046/j.1440-1754.2001.00743.x
dc.relation.references62. Heikkilä MP, Saris PEJ. Inhibition of Staphylococcus aureus by the commensal bacteria of human milk. J Appl Microbiol [Internet]. 2003 Sep;95(3):471–8. Available from: https://academic.oup.com/jambio/article/95/3/471/6723839
dc.relation.references63. Martín R, Langa S, Reviriego C, Jiménez E, Marín ML, Xaus J, et al. Human milk is a source of lactic acid bacteria for the infant gut. Journal of Pediatrics [Internet]. 2003 Dec 1 [cited 2023 Dec 18];143(6):754–8. Available from: http://www.jpeds.com/article/S0022347603006140/fulltext
dc.relation.references64. Martín R, Jime E, Heilig H, Ferna L, Marín ML, Zoetendal EG, et al. Isolation of Bifidobacteria from Breast Milk and Assessment of the Bifidobacterial Population by PCR-Denaturing Gradient Gel Electrophoresis and Quantitative Real-Time PCR ᰔ. 2009;75(4):965–9.
dc.relation.references65. Mehanna NSH, Tawfik NF, Salem MME, Effat BAM, Gad El-Rab DA. Assessment of potential probiotic bacteria isolated from breast milk. Middle East J Sci Res. 2013;14(3):354–60.
dc.relation.references66. Leila RN, Farideh T, Behzad A, Reihaneh N, Reza MH. Isolation and identification of lactic acid bacteria in mother’s breast milk in Iran using 16S ribosomal DNA sequencing method. Clin Biochem. 2011 Sep 1;44(13):S233.
dc.relation.references67. Albesharat R, Ehrmann MA, Korakli M, Yazaji S, Vogel RF. Phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies. Syst Appl Microbiol. 2011 Apr;34(2):148–55.
dc.relation.references68. Schwab C, Voney E, Ramirez Garcia A, Vischer M, Lacroix C. Characterization of the Cultivable Microbiota in Fresh and Stored Mature Human Breast Milk. Front Microbiol. 2019 Nov 20;10.
dc.relation.references69. Moossavi S, Sepehri S, Robertson B, Bode L, Goruk S, Field CJ, et al. Composition and Variation of the Human Milk Microbiota Are Influenced by Maternal and Early-Life Factors. Cell Host Microbe. 2019 Feb;25(2):324-335.e4.
dc.relation.references70. Hunt KM, Foster JA, Forney LJ, Schütte UME, Beck DL, Abdo Z, et al. Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk. PLoS One. 2011 Jun 17;6(6):e21313.
dc.relation.references71. Lackey KA, Williams JE, Meehan CL, Zachek JA, Benda ED, Price WJ, et al. What’s Normal? Microbiomes in Human Milk and Infant Feces Are Related to Each Other but Vary Geographically: The INSPIRE Study. Front Nutr. 2019 Apr 17;6.
dc.relation.references72. Lopez Leyva L, Brereton NJB, Koski KG. Emerging frontiers in human milk microbiome research and suggested primers for 16S rRNA gene analysis. Comput Struct Biotechnol J. 2021;19:121–33.
dc.relation.references73. Lyons KE, Fouhy F, O’ Shea C, Ryan CA, Dempsey EM, Ross RP, et al. Effect of storage, temperature, and extraction kit on the phylogenetic composition detected in the human milk microbiota. Microbiologyopen. 2021 Feb 29;10(1).
dc.relation.references74. Togo A, Dufour JC, Lagier JC, Dubourg G, Raoult D, Million M. Repertoire of human breast and milk microbiota: a systematic review. Future Microbiol. 2019 May;14(7):623–41.
dc.relation.references75. Boix-Amorós A, Martinez-Costa C, Querol A, Collado MC, Mira A. Multiple Approaches Detect the Presence of Fungi in Human Breastmilk Samples from Healthy Mothers. Sci Rep. 2017 Oct 12;7(1):13016.
dc.relation.references76. Mohandas S, Pannaraj PS. Beyond the Bacterial Microbiome: Virome of Human Milk and Effects on the Developing Infant. In 2020. p. 86–93.
dc.relation.references77. Morniroli D, Consales A, Crippa BL, Vizzari G, Ceroni F, Cerasani J, et al. The Antiviral Properties of Human Milk: A Multitude of Defence Tools from Mother Nature. Nutrients. 2021 Feb 22;13(2):694.
dc.relation.references78. Stinson LF, Sindi ASM, Cheema AS, Lai CT, Mühlhäusler BS, Wlodek ME, et al. The human milk microbiome: who, what, when, where, why, and how? Nutr Rev. 2021 Apr 7;79(5):529–43.
dc.relation.references79. Jeurink PV, van Bergenhenegouwen J, Jiménez E, Knippels LMJ, Fernández L, Garssen J, et al. Human milk: a source of more life than we imagine. Benef Microbes [Internet]. 2013 Mar 1;4(1):17–30. Available from: https://www.wageningenacademic.com/doi/10.3920/BM2012.0040
dc.relation.references80. Moossavi S, Azad MB. Origins of human milk microbiota: new evidence and arising questions. Gut Microbes [Internet]. 2020;12(1):1667722. Available from: https://doi.org/10.1080/19490976.2019.1667722
dc.relation.references81. Jost T, Lacroix C, Braegger CP, Rochat F, Chassard C. Vertical mother–neonate transfer of maternal gut bacteria via breastfeeding. Environ Microbiol. 2014 Sep 3;16(9):2891–904.
dc.relation.references82. Kim SY, Yi DY. Analysis of the human breast milk microbiome and bacterial extracellular vesicles in healthy mothers. Exp Mol Med. 2020 Aug 3;52(8):1288–97.
dc.relation.references83. Fernández L, Langa, Susana MV et al. The human milk microbiota: Origin and potential roles in health and disease. Pharmacol Res [Internet]. 2013;69(1):1–10. Available from: http://dx.doi.org/10.1016/j.phrs.2012.09.001
dc.relation.references84. Cabrera-Rubio R, Mira-Pascual L, Mira A, Collado MC. Impact of mode of delivery on the milk microbiota composition of healthy women. J Dev Orig Health Dis. 2016 Feb 19;7(1):54–60.
dc.relation.references85. Hermansson H, Kumar H, Collado MC, Salminen S, Isolauri E, Rautava S. Breast Milk Microbiota Is Shaped by Mode of Delivery and Intrapartum Antibiotic Exposure. Front Nutr. 2019 Feb 4;6.
dc.relation.references86. Li SW, Watanabe K, Hsu CC, Chao SH, Yang ZH, Lin YJ, et al. Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China. Front Microbiol. 2017 May 30;8.
dc.relation.references87. Cortes-Macías E, Selma-Royo M, García-Mantrana I, Calatayud M, González S, Martínez-Costa C, et al. Maternal Diet Shapes the Breast Milk Microbiota Composition and Diversity: Impact of Mode of Delivery and Antibiotic Exposure. J Nutr. 2021 Feb;151(2):330–40.
dc.relation.references88. Xi M, Na X, Ma X, Lan H, Sun T, Liu WH, et al. Maternal diet associated with infants’ intestinal microbiota mediated by predominant long-chain fatty acid in breast milk. Front Microbiol. 2023 Jan 6;13.
dc.relation.references89. Taylor R, Keane D, Borrego P, Arcaro K. Effect of Maternal Diet on Maternal Milk and Breastfed Infant Gut Microbiomes: A Scoping Review. Nutrients. 2023 Mar 15;15(6):1420.
dc.relation.references90. Williams JE, Carrothers JM, Lackey KA, Beatty NF, York MA, Brooker SL, et al. Human Milk Microbial Community Structure Is Relatively Stable and Related to Variations in Macronutrient and Micronutrient Intakes in Healthy Lactating Women. J Nutr. 2017 Sep 1;147(9):1739–48.
dc.relation.references91. Padilha M, Danneskiold-Samsøe NB, Brejnrod A, Hoffmann C, Cabral VP, Iaucci J de M, et al. The Human Milk Microbiota is Modulated by Maternal Diet. Microorganisms [Internet]. 2019 Nov 1 [cited 2023 Dec 22];7(11). Available from: https://pubmed.ncbi.nlm.nih.gov/31671720/
dc.relation.references92. Ranjha MMAN, Shafique B, Batool M, Kowalczewski PŁ, Shehzad Q, Usman M, et al. Nutritional and Health Potential of Probiotics: A Review. Applied Sciences. 2021 Nov 25;11(23):11204.
dc.relation.references93. Anee IJ, Alam S, Begum RA, Shahjahan RM, Khandaker AM. The role of probiotics on animal health and nutrition. The Journal of Basic and Applied Zoology. 2021 Dec 29;82(1):52.
dc.relation.references94. Liu W, Pang H, Zhang H, Cai Y. Biodiversity of Lactic Acid Bacteria. In: Lactic Acid Bacteria. Dordrecht: Springer Netherlands; 2014. p. 103–203.
dc.relation.references95. Wang Y, Wu J, Lv M, Shao Z, Hungwe M, Wang J, et al. Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Front Bioeng Biotechnol. 2021 May 12;9.
dc.relation.references96. Raman J, Kim JS, Choi KR, Eun H, Yang D, Ko YJ, et al. Application of Lactic Acid Bacteria (LAB) in Sustainable Agriculture: Advantages and Limitations. Int J Mol Sci. 2022 Jul 14;23(14):7784.
dc.relation.references97. Zapaśnik A, Sokołowska B, Bryła M. Role of Lactic Acid Bacteria in Food Preservation and Safety. Foods. 2022 Apr 28;11(9):1283.
dc.relation.references98. Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of Action of Probiotics. Advances in Nutrition. 2019 Jan;10:S49–66.
dc.relation.references99. Luo Y, De Souza C, Ramachandran M, Wang S, Yi H, Ma Z, et al. Precise oral delivery systems for probiotics: A review. Journal of Controlled Release. 2022 Dec;352:371–84.
dc.relation.references100. Krausova G, Hyrslova I, Hynstova I. In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains. Fermentation. 2019 Dec 4;5(4):100.
dc.relation.references101. Gorreja F, Walker WA. The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics: a narrative review of experimental and human studies. Gut Microbes. 2022 Dec 31;14(1).
dc.relation.references102. Wang S, Li L, Yu L, Tian F, Zhao J, Zhai Q, et al. Natural aggregation of Lactobacillus: Mechanisms and influencing factors. Food Biosci. 2024 Dec;62:105007.
dc.relation.references103. Fijan S. Probiotics and Their Antimicrobial Effect. Microorganisms. 2023 Feb 19;11(2):528.
dc.relation.references104. Montassier E, Valdés-Mas R, Batard E, Zmora N, Dori-Bachash M, Suez J, et al. Probiotics impact the antibiotic resistance gene reservoir along the human GI tract in a person-specific and antibiotic-dependent manner. Nat Microbiol. 2021;6(8):1043–54.
dc.relation.references105. DI W, ZHANG YC, YI HX, HAN X, WANG SM, ZHANG LW. Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides. Chinese Journal of Analytical Chemistry. 2018;46(6):875–82.
dc.relation.references106. Angelin J, Kavitha M. Exopolysaccharides from probiotic bacteria and their health potential. Int J Biol Macromol. 2020 Nov;162:853–65.
dc.relation.references107. Mirzaei R, Afaghi A, Babakhani S, Sohrabi MR, Hosseini-Fard SR, Babolhavaeji K, et al. Role of microbiota-derived short-chain fatty acids in cancer development and prevention. Biomedicine & Pharmacotherapy. 2021 Jul;139:111619.
dc.relation.references108. Fu X, Liu Z, Zhu C, Mou H, Kong Q. Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria. Crit Rev Food Sci Nutr. 2019;59(0):S130–52.
dc.relation.references109. Osmanagaoglu O, Kiran F, Ataoglu H. Evaluation of in vitro Probiotic Potential of Pediococcus pentosaceus OZF Isolated from Human Breast Milk. Probiotics Antimicrob Proteins. 2010 Oct 13;2(3):162–74.
dc.relation.references110. Reis NA, Saraiva MAF, Duarte EAA, de Carvalho EA, Vieira BB, Evangelista-Barreto NS. Probiotic properties of lactic acid bacteria isolated from human milk. J Appl Microbiol. 2016 Sep;121(3):811–20.
dc.relation.references111. Srikham K, Daengprok W, Niamsup P, Thirabunyanon M. Characterization of Streptococcus salivarius as New Probiotics Derived From Human Breast Milk and Their Potential on Proliferative Inhibition of Liver and Breast Cancer Cells and Antioxidant Activity. Front Microbiol. 2021 Dec 15;12.
dc.relation.references112. Maldonado J, Gil-Campos M, Maldonado-Lobón JA, Benavides MR, Flores-Rojas K, Jaldo R, et al. Evaluation of the safety, tolerance and efficacy of 1-year consumption of infant formula supplemented with Lactobacillus fermentum CECT5716 Lc40 or Bifidobacterium breve CECT7263: a randomized controlled trial. BMC Pediatr. 2019 Dec 21;19(1):361.
dc.relation.references113. Lagkouvardos I, Intze E, Schaubeck M, Rooney JPK, Hecht C, Piloquet H, et al. Early life gut microbiota profiles linked to synbiotic formula effects: a randomized clinical trial in European infants. Am J Clin Nutr. 2023 Feb;117(2):326–39.
dc.relation.references114. Olivares M, Díaz-Ropero MP, Gómez N, Lara-Villoslada F, Sierra S, Maldonado JA, et al. The consumption of two new probiotic strains, Lactobacillus gasseri CECT 5714 and Lactobacillus coryniformis CECT 5711, boosts the immune system of healthy humans. Int Microbiol. 2006 Mar;9(1):47–52.
dc.relation.references115. Olivares M, Díaz-Ropero MP, Gómez N, Lara-Villoslada F, Sierra S, Maldonado JA, et al. Oral administration of two probiotic strains, Lactobacillus gasseri CECT5714 and Lactobacillus coryniformis CECT5711, enhances the intestinal function of healthy adults. Int J Food Microbiol. 2006 Mar;107(2):104–11.
dc.relation.references116. Martínez‐Cañavate A, Sierra S, Lara‐Villoslada F, Romero J, Maldonado J, Boza J, et al. A probiotic dairy product containing L. gasseriCECT5714 and L. coryniformis CECT5711 induces immunological changes in children suffering from allergy. Pediatric Allergy and Immunology. 2009 Sep 20;20(6):592–600.
dc.relation.references117. Chiu YH, Tsai JJ, Lin SL, Chotirosvakin C, Lin MY. Characterisation of bifidobacteria with immunomodulatory properties isolated from human breast milk. J Funct Foods. 2014 Mar;7:700–8.
dc.relation.references118. Khalkhali S, Mojgani N. In vitro and in vivo safety analysis of Enterococcus faecium 2C isolated from human breast milk. Microb Pathog. 2018 Mar;116:73–7.
dc.relation.references119. Riaz Rajoka MS, Zhao H, Lu Y, Lian Z, Li N, Hussain N, et al. Anticancer potential against cervix cancer (HeLa) cell line of probiotic Lactobacillus casei and Lactobacillus paracasei strains isolated from human breast milk. Food Funct [Internet]. 2018;9(5):2705–15. Available from: http://xlink.rsc.org/?DOI=C8FO00547H
dc.relation.references120. Díaz-Ropero MP, Martín R, Sierra S, Lara-Villoslada F, Rodríguez JM, Xaus J, et al. Two Lactobacillus strains, isolated from breast milk, differently modulate the immune response. J Appl Microbiol. 2007 Feb;102(2).
dc.relation.references121. Pérez-Cano FJ, Dong H, Yaqoob P. In vitro immunomodulatory activity of Lactobacillus fermentum CECT5716 and Lactobacillus salivarius CECT5713: two probiotic strains isolated from human breast milk. Immunobiology. 2010 Dec;215(12):996–1004.
dc.relation.references122. Pastor-Villaescusa B, Hurtado JA, Gil-Campos M, Uberos J, Maldonado-Lobón JA, Díaz-Ropero MP, et al. Effects of Lactobacillus fermentum CECT5716 Lc40 on infant growth and health: a randomised clinical trial in nursing women. Benef Microbes. 2020 May 11;11(3):235–44.
dc.relation.references123. Lara-Villoslada F, Sierra S, Boza J, Xaus J, Olivares M. Beneficial effects of consumption of a dairy product containing two probiotic strains, Lactobacillus coryniformis CECT5711 and Lactobacillus gasseri CECT5714 in healthy children. Nutr Hosp. 2007;22(4):496–502.
dc.relation.references124. Kang JH, Yun SI, Park MH, Park JH, Jeong SY, Park HO. Anti-Obesity Effect of Lactobacillus gasseri BNR17 in High-Sucrose Diet-Induced Obese Mice. PLoS One. 2013 Jan 30;8(1):e54617.
dc.relation.references125. Hu P, Sun J, Gao R, Li K, Liu J, Pan X, et al. Harnessing the power of breast milk: how Lactiplantibacillus plantarum FN029 from rural western China mitigates severe atopic dermatitis in mice through retinol metabolism activation. Food Funct. 2025;
dc.relation.references126. Li N, Pang B, Liu G, Zhao X, Xu X, Jiang C, et al. Lactobacillus rhamnosus from human breast milk shows therapeutic function against foodborne infection by multi-drug resistant Escherichia coli in mice. Food Funct. 2020;11(1):435–47.
dc.relation.references127. Yu J, Li W, Xu R, Liu X, Gao G, Kwok L, et al. Probio‐M9, a breast milk‐originated probiotic, alleviates mastitis and enhances antibiotic efficacy: Insights into the gut–mammary axis. iMeta. 2024 Aug 9;3(4).
dc.relation.references128. Luo Z, Sun J, Liu J, Yu P, Ye D, Qi C, et al. Human milk-derived Limosilactobacillus reuteri FN041 ameliorates DSS-induced colitis by remodeling gut microbiota and metabolites in mice. Food Biosci. 2025 Jan;63:105736.
dc.relation.references129. Peran L, Camuesco D, Comalada M, Nieto A, Concha A, Diaz-Ropero MP, et al. Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis. World J Gastroenterol. 2005 Sep 7;11(33):5185–92.
dc.relation.references130. Cárdenas N, Martín V, Arroyo R, López M, Carrera M, Badiola C, et al. Prevention of Recurrent Acute Otitis Media in Children Through the Use of Lactobacillus salivarius PS7, a Target-Specific Probiotic Strain. Nutrients. 2019 Feb 12;11(2):376.
dc.relation.references131. Garcia Rodenas CL, Lepage M, Ngom‐Bru C, Fotiou A, Papagaroufalis K, Berger B. Effect of Formula Containing Lactobacillus reuteri DSM 17938 on Fecal Microbiota of Infants Born by Cesarean‐Section. J Pediatr Gastroenterol Nutr. 2016 Dec;63(6):681–7.
dc.relation.references132. Fatheree NY, Liu Y, Taylor CM, Hoang TK, Cai C, Rahbar MH, et al. Lactobacillus reuteri for Infants with Colic: A Double-Blind, Placebo-Controlled, Randomized Clinical Trial. J Pediatr. 2017 Dec;191:170-178.e2.
dc.relation.references133. Kosek MN, Peñataro-Yori P, Paredes-Olortegui M, Lefante J, Ramal-Asayag C, Zamora-Babilonia M, et al. Safety of Lactobacillus Reuteri DSM 17938 in Healthy Children 2–5 Years of Age. Pediatric Infectious Disease Journal. 2019 Aug;38(8):e178–80.
dc.relation.references134. Rodríguez-Sojo MJ, Ruiz-Malagón AJ, Rodríguez-Cabezas ME, Gálvez J, Rodríguez-Nogales A. Limosilactobacillus fermentum CECT5716: Mechanisms and Therapeutic Insights. Nutrients. 2021 Mar 21;13(3):1016.
dc.relation.references135. Ozen M, Piloquet H, Schaubeck M. Limosilactobacillus fermentum CECT5716: Clinical Potential of a Probiotic Strain Isolated from Human Milk. Nutrients. 2023 May 6;15(9):2207.
dc.relation.references136. Islam MdZ, Uddin MdE, Rahman MdT, Islam MA, Harun-ur-Rashid Md. Isolation and characterization of dominant lactic acid bacteria from raw goat milk: Assessment of probiotic potential and technological properties. Small Ruminant Research. 2021 Dec;205:106532.
dc.relation.references137. Zhang F, Jiang M, Wan C, Chen X, Chen X, Tao X, et al. Screening probiotic strains for safety: Evaluation of virulence and antimicrobial susceptibility of enterococci from healthy Chinese infants. J Dairy Sci. 2016 Jun;99(6):4282–90.
dc.relation.references138. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2012 Nov 27;41(D1):D590–6.
dc.relation.references139. Tamura K, Stecher G, Kumar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 2021 Jun 25;38(7):3022–7.
dc.relation.references140. Monteiro ACM, Fortaleza CMCB, Ferreira AM, Cavalcante R de S, Mondelli AL, Bagagli E, et al. Comparison of methods for the identification of microorganisms isolated from blood cultures. Ann Clin Microbiol Antimicrob. 2016 Dec 5;15(1):45.
dc.relation.references141. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 2011 May 2;17(1):10.
dc.relation.references142. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities. Appl Environ Microbiol. 2009 Dec;75(23):7537–41.
dc.relation.references143. Rognes T, Flouri T, Nichols B, Quince C, Mahé F. VSEARCH: a versatile open source tool for metagenomics. PeerJ. 2016 Oct 18;4:e2584.
dc.relation.references144. Bedoya K, Hoyos O, Zurek E, Cabarcas F, Alzate JF. Annual microbial community dynamics in a full-scale anaerobic sludge digester from a wastewater treatment plant in Colombia. Science of The Total Environment. 2020 Jul;726:138479.
dc.relation.references145. Huligere SS, Chandana Kumari VB, Alqadi T, Kumar S, Cull CA, Amachawadi RG, et al. Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters. Front Microbiol. 2023 Jan 23;13.
dc.relation.references146. da Silva LA, Lopes Neto JHP, Cardarelli HR. Safety and probiotic functionality of isolated goat milk lactic acid bacteria. Ann Microbiol. 2019 Dec 2;69(13):1497–505.
dc.relation.references147. Rychen G, Aquilina G, Azimonti G, Bampidis V, Bastos M de L, Bories G, et al. Guidance on the characterisation of microorganisms used as feed additives or as production organisms. EFSA Journal. 2018 Mar;16(3).
dc.relation.references148. Hussin M, Anzian A, Liew CXQ, Muhialdin BJ, Mohsin AZ, Fang CM, et al. Potentially Probiotic Fermented Glutinous Rice (Oryza sativa L.) with Lactiplantibacillus plantarum Improved Immune System Response in a Small Sample of BALB/cByJ Mice. Fermentation. 2022 Nov 8;8(11):612.
dc.relation.references149. Amenu D, Bacha K. Probiotic potential and safety analysis of lactic acid bacteria isolated from Ethiopian traditional fermented foods and beverages. Ann Microbiol. 2023 Oct 26;73(1):37.
dc.relation.references150. Divyashree S, Ramu R, Sreenivasa MY. Evaluation of new candidate probiotic lactobacillus strains isolated from a traditional fermented food- multigrain-millet dosa batter. Food Biosci. 2024 Feb;57:103450.
dc.relation.references151. Rodríguez-Sánchez S, Fernández-Pacheco P, Seseña S, Pintado C, Palop ML. Selection of probiotic Lactobacillus strains with antimicrobial activity to be used as biocontrol agents in food industry. LWT. 2021 May;143:111142.
dc.relation.references152. Sadeghi M, Panahi B, Mazlumi A, Hejazi MA, Komi DEA, Nami Y. Screening of potential probiotic lactic acid bacteria with antimicrobial properties and selection of superior bacteria for application as biocontrol using machine learning models. LWT. 2022 Jun;162:113471.
dc.relation.references153. Norma ISO 10993:2009. Biological Evaluation of Medical Devices – Part 5: Test for in vitro cytotoxicity [Internet]. 2009. Available from: www.aenor.esTel.:902Fax:913
dc.relation.references154. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods [Internet]. 1983 Dec 16 [cited 2025 Aug 21];65(1–2):55–63. Available from: https://pubmed.ncbi.nlm.nih.gov/6606682/
dc.relation.references155. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications: Sixth Edition. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications: Sixth Edition [Internet]. 2011 Mar 9 [cited 2025 Aug 21];394. Available from: https://onlinelibrary.wiley.com/doi/book/10.1002/9780470649367
dc.relation.references156. Nambiar RB, Sellamuthu PS, Perumal AB, Sadiku ER, Phiri G, Jayaramudu J. Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk. Process Biochemistry. 2018 Oct;73:15–22.
dc.relation.references157. Hati S, Patel M, Mishra BK, Das S. Short-chain fatty acid and vitamin production potentials of Lactobacillus isolated from fermented foods of Khasi Tribes, Meghalaya, India. Ann Microbiol. 2019;69(11):1191–9.
dc.relation.references158. Altschul S. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997 Sep 1;25(17):3389–402.
dc.relation.references159. de Lillo A, Ashley FP, Palmer RM, Munson MA, Kyriacou L, Weightman AJ, et al. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol Immunol. 2006 Feb 3;21(1):61–8.
dc.relation.references160. Hall T. BioEdit: biological equence alignment editor for Win95/98/NT/2K/XP. URL http://www.mbio.ncsu.edu/bioedit.html. 2005.
dc.relation.references161. Minh BQ, Nguyen MAT, von Haeseler A. Ultrafast Approximation for Phylogenetic Bootstrap. Mol Biol Evol. 2013 May 1;30(5):1188–95.
dc.relation.references162. Asociación Médica Mundial. Declaración de HELSINKI de la AMM. 2009;
dc.relation.references163. Davis C. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria. J Microbiol Methods. 2014 Aug;103:9–17.
dc.relation.references164. Paul Vos, George M. Garrity, Dorothy Jones, Noel R. Krieg, Wolfgang Ludwig, Fred A. Rainey, et al. Bergey’s Manual of Systematic Bacteriology [Internet]. 2nd ed. Whitman WB, editor. Vol. 3. New York, NY: Springer New York; 2009. LII, 1450. Available from: http://link.springer.com/10.1007/978-0-387-68489-5
dc.relation.references165. Łubiech K, Twarużek M. Lactobacillus Bacteria in Breast Milk. Nutrients. 2020 Dec 10;12(12):3783.
dc.relation.references166. Sharma A, Lee S, Park YS. Molecular typing tools for identifying and characterizing lactic acid bacteria: a review. Food Sci Biotechnol. 2020 Oct 16;29(10):1301–18.
dc.relation.references167. Jost T, Lacroix C, Braegger C, Chassard C. Assessment of bacterial diversity in breast milk using culture-dependent and culture-independent approaches. British Journal of Nutrition. 2013 Oct 14;110(7):1253–62.
dc.relation.references168. Ma J, Wang W, Sun C, Gu L, Liu Z, Yu W, et al. Effects of environmental stresses on the physiological characteristics, adhesion ability and pathogen adhesion inhibition of Lactobacillus plantarum KLDS 1.0328. Process Biochemistry. 2020 May;92:426–36.
dc.relation.references169. Wang K, Xia X, Sun L, Wang H, Li Q, Yang Z, et al. Microbial Diversity and Correlation between Breast Milk and the Infant Gut. Foods. 2023 Apr 22;12(9):1740.
dc.relation.references170. Zawistowska-Rojek A, Zaręba T, Tyski S. Microbiological Testing of Probiotic Preparations. Int J Environ Res Public Health. 2022 May 7;19(9):5701.
dc.relation.references171. Moraes PM, Perin LM, Silva Júnior A, Nero LA. Comparison of phenotypic and molecular tests to identify lactic acid bacteria. Brazilian Journal of Microbiology. 2013;44(1):109–12.
dc.relation.references172. Krawczyk B, Wityk P, Gałęcka M, Michalik M. The Many Faces of Enterococcus spp.-Commensal, Probiotic and Opportunistic Pathogen. Microorganisms. 2021 Sep 7;9(9).
dc.relation.references173. Kapse N, Pisu V, Dhakephalkar T, Margale P, Shetty D, Wagh S, et al. Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In Vitro Studies Corroborated with Genome Analysis. Microorganisms. 2024 Feb 7;12(2):347.
dc.relation.references174. World Health Organization. Antimicrobial resistance: global report on surveillance. 2014.
dc.relation.references175. Qi Y, Huang L, Zeng Y, Li W, Zhou D, Xie J, et al. Pediococcus pentosaceus: Screening and Application as Probiotics in Food Processing. Front Microbiol. 2021 Dec 16;12.
dc.relation.references176. Mgomi FC, Yuan L, Wang Y, Rao S, Yang Z. Physiological properties, survivability and genomic characteristics of Pediococcus pentosaceus for application as a starter culture. Int J Dairy Technol. 2022 Aug 28;75(3):588–602.
dc.relation.references177. Li Y, Zhang Y, Zhao J, Zhang X, Liu S, Qi H, et al. Isolation and evaluation of Pediococcus acidilactici YH-15 from cat milk: Potential probiotic effects and antimicrobial properties. Heliyon. 2024 Oct;10(20):e39539.
dc.relation.references178. Hou X, Wang M, Hu T, Wu Z, Liang H, Zhong Y, et al. Evaluation of the safety and probiotic properties of Limosilactobacillus fermentum BGI-AF16, a uric acid-lowering probiotic strain. Microb Pathog. 2025 Apr;201:107382.
dc.relation.references179. Khoo SC, Chin KW, Ting TZ, Luang-In V, Chi-Wei Lan J, Ma NL. Stress tolerance and metabolism profiling of selected functional probiotic strains. Food Biosci. 2025 Feb;64:105919.
dc.relation.references180. Yang Y, Song X, Wang G, Xia Y, Xiong Z, Ai L. Understanding Ligilactobacillus salivarius from Probiotic Properties to Omics Technology: A Review. Foods. 2024 Mar 15;13(6):895.
dc.relation.references181. Wang C, Cui Y, Qu X. Mechanisms and improvement of acid resistance in lactic acid bacteria. Arch Microbiol. 2018 Mar 26;200(2):195–201.
dc.relation.references182. Hernández-Alcántara AM, Wacher C, Llamas MG, López P, Pérez-Chabela ML. Probiotic properties and stress response of thermotolerant lactic acid bacteria isolated from cooked meat products. LWT. 2018 May;91:249–57.
dc.relation.references183. Bellesi FA, Pilosof AMR. Potential implications of food proteins-bile salts interactions. Food Hydrocoll. 2021 Sep;118:106766.
dc.relation.references184. Hao H, Nie Z, Wu Y, Liu Z, Luo F, Deng F, et al. Probiotic Characteristics and Anti-Inflammatory Effects of Limosilactobacillus fermentum 664 Isolated from Chinese Fermented Pickles. Antioxidants. 2024 Jun 7;13(6):703.
dc.relation.references185. Escobar-Sánchez M, Carrasco-Navarro U, Juárez-Castelán C, Lozano-Aguirre Beltrán L, Pérez-Chabela ML, Ponce-Alquicira E. Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101. Foods. 2022 Dec 22;12(1):46.
dc.relation.references186. Guan N, Liu L. Microbial response to acid stress: mechanisms and applications. Appl Microbiol Biotechnol. 2020 Jan 26;104(1):51–65.
dc.relation.references187. Loo JS, Oslan SNH, Mokshin NAS, Othman R, Amin Z, Dejtisakdi W, et al. Comprehensive Review of Strategies for Lactic Acid Bacteria Production and Metabolite Enhancement in Probiotic Cultures: Multifunctional Applications in Functional Foods. Fermentation. 2025 Apr 24;11(5):241.
dc.relation.references188. World Health Organization. Estimaciones de la OMS sobre la carga mundial de enfermedades de transmisión alimentaria [Internet]. Vol. 14, World Health Organization. 2015. Available from: http://apps.who.int/iris/bitstream/10665/200047/1/WHO_FOS_15.02_spa.pdf
dc.relation.references189. World Health Organization. Medidas para reforzar la inocuidad de los alimentos. 2020 Oct 3;
dc.relation.references190. Zawistowska-Rojek A, Kośmider A, Stępień K, Tyski S. Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria. Arch Microbiol. 2022 May 27;204(5):285.
dc.relation.references191. Han Q, Kong B, Chen Q, Sun F, Zhang H. In vitro comparison of probiotic properties of lactic acid bacteria isolated from Harbin dry sausages and selected probiotics. J Funct Foods. 2017 May;32:391–400.
dc.relation.references192. Roldán-Pérez S, Gómez Rodríguez SL, Sepúlveda-Valencia JU, Ruiz Villadiego OS, Márquez Fernández ME, Montoya Campuzano OI, et al. Assessment of probiotic properties of lactic acid bacteria isolated from an artisanal Colombian cheese. Heliyon. 2023 Nov;9(11):e21558.
dc.relation.references193. Somashekaraiah R, Shruthi B, Deepthi B V., Sreenivasa MY. Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar. Front Microbiol. 2019 Jun 28;10.
dc.relation.references194. Darmastuti A, Hasan PN, Wikandari R, Utami T, Rahayu ES, Suroto DA. Adhesion Properties of Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 on Sprague Dawley Rat Intestine. Microorganisms. 2021 Nov 11;9(11):2336.
dc.relation.references195. Li SJ, So JS. In Vitro Characterization of Cell Surface Properties of 14 Vaginal Lactobacillus Strains as Potential Probiotics. Adv Microbiol. 2021;11(02):144–55.
dc.relation.references196. Cisneros L, Cattelan N, Villalba MI, Rodriguez C, Serra DO, Yantorno O, et al. Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization. Lett Appl Microbiol. 2021 Aug 3;73(2):247–56.
dc.relation.references197. Gharbi Y, Fhoula I, Ruas-Madiedo P, Afef N, Boudabous A, Gueimonde M, et al. In-vitro characterization of potentially probiotic Lactobacillus strains isolated from human microbiota: interaction with pathogenic bacteria and the enteric cell line HT29. Ann Microbiol. 2019 Jan 8;69(1):61–72.
dc.relation.references198. Fonseca HC, de Sousa Melo D, Ramos CL, Dias DR, Schwan RF. Probiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells. Probiotics Antimicrob Proteins. 2021 Feb 15;13(1):102–12.
dc.relation.references199. Sim EA, Kim SY, Kim S, Mun EG. Probiotic Potential and Enhanced Adhesion of Fermented Foods-Isolated Lactic Acid Bacteria to Intestinal Epithelial Caco-2 and HT-29 Cells. Microorganisms. 2024 Dec 27;13(1):32.
dc.relation.references200. Duary RK, Rajput YS, Batish VK, Grover S. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Indian Journal of Medical Research. 2011 Nov;134(5):664–71.
dc.relation.references201. Vasiee A, Falah F, Behbahani BA, Tabatabaee-yazdi F. Probiotic characterization of Pediococcus strains isolated from Iranian cereal-dairy fermented product: Interaction with pathogenic bacteria and the enteric cell line Caco-2. J Biosci Bioeng. 2020 Nov;130(5):471–9.
dc.relation.references202. Guan C, Chen X, Jiang X, Zhao R, Yuan Y, Chen D, et al. In vitro studies of adhesion properties of six lactic acid bacteria isolated from the longevous population of China. RSC Adv. 2020;10(41):24234–40.
dc.relation.references203. Shirvanian M, Azimian Zavareh V, Zamanzadeh Z, Janghorban M, Mohammadi E, Ahangarzadeh S. Probiotic Potential, Antiproliferative, and Proapoptotic Activities of Lactobacillus fermentum Isolated from Breast Milk. Adv Biomed Res. 2023 Oct;12(1).
dc.relation.references204. Wei G, Wang D, Wang T, Wang G, Chai Y, Li Y, et al. Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production. Front Microbiol. 2025 Jan 21;16.
dc.relation.references205. Bikric S, Aslim B, Dincer İ, Yuksekdag Z, Ulusoy S, Yavuz S. Characterization of Exopolysaccharides (EPSs) Obtained from Ligilactobacillus salivarius Strains and Investigation at the Prebiotic Potential as an Alternative to Plant Prebiotics at Poultry. Probiotics Antimicrob Proteins. 2022 Feb 29;14(1):49–59.
dc.relation.references206. Bai Y, Luo B, Zhang Y, Li X, Wang Z, Shan Y, et al. Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties. Int J Biol Macromol. 2021 Aug;185:1036–49.
dc.relation.references207. Jiang G, He J, Gan L, Li X, Xu Z, Yang L, et al. Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application. Front Microbiol. 2022 Jun 22;13.
dc.relation.references208. Prete R, Alam MK, Perpetuini G, Perla C, Pittia P, Corsetti A. Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods. Foods. 2021 Jul 17;10(7):1653.
dc.relation.references209. Yadav MK, Song JH, Vasquez R, Lee JS, Kim IH, Kang DK. Methods for Detection, Extraction, Purification, and Characterization of Exopolysaccharides of Lactic Acid Bacteria—A Systematic Review. Foods. 2024 Nov 19;13(22):3687.
dc.relation.references210. Lee MG, Joeng H, Shin J, Kim S, Lee C, Song Y, et al. Potential Probiotic Properties of Exopolysaccharide-Producing Lacticaseibacillus paracasei EPS DA-BACS and Prebiotic Activity of Its Exopolysaccharide. Microorganisms. 2022 Dec 8;10(12):2431.
dc.relation.references211. Silva YP, Bernardi A, Frozza RL. The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication. Front Endocrinol (Lausanne). 2020 Jan 31;11
dc.relation.references212. Calvigioni M, Bertolini A, Codini S, Mazzantini D, Panattoni A, Massimino M, et al. HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains. Front Microbiol. 2023 Mar 3;14.
dc.relation.references213. Thananimit S, Pahumunto N, Teanpaisan R. Characterization of Short Chain Fatty Acids Produced by Selected Potential Probiotic Lactobacillus Strains. Biomolecules. 2022 Dec 7;12(12):1829.
dc.relation.references214. Punia Bangar S, Suri S, Trif M, Ozogul F. Organic acids production from lactic acid bacteria: A preservation approach. Food Biosci. 2022 Apr;46:101615.
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc660 - Ingeniería química
dc.subject.lembLactación
dc.subject.lembAcido láctico
dc.subject.lembBacterias
dc.subject.proposalMicrobiotaspa
dc.subject.proposalLeche maternaspa
dc.subject.proposalBacterias ácido lácticasspa
dc.subject.proposalProbióticosspa
dc.subject.proposalSalud infantilspa
dc.subject.proposalMicrobiotaeng
dc.subject.proposalHuman breast milkeng
dc.subject.proposalLactic acid bacteriaeng
dc.subject.proposalProbioticseng
dc.subject.proposalChild healtheng
dc.titlePotencial probiótico de bacterias ácido lácticas aisladas de leche materna en Antioquiaspa
dc.title.translatedProbiotic potential of lactic acid bacteria isolated from human breast milk in Antioquiaeng
dc.typeTrabajo de grado - Doctorado
dc.type.coarhttp://purl.org/coar/resource_type/c_beb9
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/doctoralThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TD
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dcterms.audience.professionaldevelopmentInvestigadores
dcterms.audience.professionaldevelopmentEstudiantes
dcterms.audience.professionaldevelopmentMaestros
dcterms.audience.professionaldevelopmentEspecializada
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2

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