In vitro modeling of mechanical and biochemical aspects involved in the cellular migratory processes related with colorectal cancer metastasis

Vista sencilla de ítem
dc.contributor.advisorOchoa Garrido, Ignaciospa
dc.contributor.advisorDoblaré Castellano, Manuelspa
dc.contributor.advisorGarzón Alvarado, Diego Alexanderspa
dc.contributor.authorCastro Abril, Hector Alfonsospa
dc.contributor.researchgrouplaboratorio de Biomiméticos: Grupo de Mecanobiología de Órganos y Tejidosspa
dc.contributor.researchgroupTME Lab (Universidad de Zaragoza)spa
dc.date.accessioned2024-03-12T19:07:21Z
dc.date.available2024-03-12T19:07:21Z
dc.date.issued2023-12
dc.descriptionilustraciones, diagramasspa
dc.description.abstractMetastasis is one of the most lethal manifestations in cancer, accounting for up to 90% of cancer related deaths. Recently, it has been shown that the mechanics of tissues, as well as the biological response of cells to different biophysical stimuli, play an active role in the development of cancer as well as in metastasis. Since this disease is a major concern in public health, understanding the biology of metastasis and how mechanics affect this process is essential to develop new therapeutic strategies to attack and even prevent it. The main aim of this thesis was to study the role of the mechanical environment and the nutrient availability in the evolution of Colorectal Cancer (CRC), one of the leading causes of cancer deaths worldwide. For this, we first varied different fabrication parameters of previously established pro tocols for generating type I collagen hydrogels. With these hydrogels, we studied the migrating behavior of CRC cell-line spheroids and found that the combined effect of the ultrastructure of the hydrogels and their mechanical stiffness generated profound distinct patterns in cell invasion. In a parallel approach, we analyzed the response of spheroids to dynamic changes in glucose and Fetal Bovine Serum (FBS) availability in order to check whether these changes generated an adaptive response in the samples. Our results showed that the spheroids indeed had the ability to adapt to nutrient-deprived environments, especially after several cycles of nutrient-abundance and nutrient-starvation. Furthermore, in the case of the HCT-116 cell line, we observed that the spheroids expelled their necrotic core during the starvation cycles, a striking phenomenon that, to the best of our knowledge, has never been reported in literature. Due to the notoriety of the event, we explored, in the last part of this thesis, the possible mech anisms involved in the ejection of the core. For this, we explored different cytoskeleton (CSK) blockers in the preconditioned spheroids during the starvation periods to check whether the ejec tion of the core was related to cell contraction. This approach allowed us to conclude that it was indeed involved in the phenomenon, since blocked spheroids did not eject their core during the mentioned period.eng
dc.description.abstractLa metástasis es una de las manifestaciones más letales del cáncer, con un estimado de 90% de muertes relacionadas por cáncer. Recientemente, se ha demostrado que la mecánica de los tejidos y la respuesta biológica de las células ante distintos estímulos biofísicos juegan un papel activo en el desarrollo del cáncer y la metástasis. Dado que esta enfermedad es un problema de salud pública, el entendimiento de la biología de la metástasis y la forma en la que la mecánica afecta este proceso es esencial para desarrollar nuevas estrategias terapéuticas para atacarla e incluso prevenirla. El objetivo principal de esta tesis consistió en estudiar el rol del entorno mecánico y la disponibilidad de nutrientes en la evolución del cáncer colorrectal (CRC por sus siglas en inglés), una de las principales causas de muerte por cáncer en el mundo. Para esto, se variaron diferentes parámetros de fabricación de hidrogeles de colágeno tipo I previamente descritos en literatura. Con estos hidrogeles, se estudió el comportamiento migratorio de esferoides celulares derivados de líneas celulares de CRC y se encontró que el efecto combinado de la ultraestructura de los hidrogeles y de su rigidez mecánica generaban patrones invasivos profundamente distintos. En una aproximación paralela, se analizó la respuesta de los esferoides ante cambios dinámicos en la disponibilidad de glucosa o suero fetal bovino (FBS, por sus siglas en inglés) con el fin de estudiar la potencial respuesta adaptativa de las muestras ante dichas variaciones. Los resultados demostraron que los esferoides efectivamente tenían la habilidad de adaptarse a entornos privados de nutrientes, especialmente después de varios ciclos de abundancia e inanición. De manera adicional, se observó que, en el caso de la línea celular HCT-116, los esferoides expulsaron su núcleo necrótico durante los ciclos de deprivación, un fenómeno que, en lo que a los autores concierne, nunca ha sido reportado en literatura. Debido a la notoriedad de este evento se exploró, en la última parte de esta tesis, los mecanismos posiblemente involucrados en la eyección del núcleo. Para esto, se exploraron diferentes bloqueadores del citoesqueleto en esferoides durante los períodos de inanición para comprobar la relación entre la expulsión y la contracción celular. Este enfoque permitió concluir que efectivamente había un vínculo, pues los esferoides tratados no eyectaron su núcleo durante los ciclos mencionados. (Texto tomado de la fuente).spa
dc.description.degreelevelDoctoradospa
dc.description.degreenameDoctor en Ingenieríaspa
dc.description.notesTesis de doble titulación con la Universidad de Zaragozaspa
dc.description.sponsorshipColciencias - Convocatoria 785 de Doctorados Nacionalesspa
dc.format.extentxxvi, 198 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85800
dc.language.isoengspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Ingenieríaspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ingeniería - Doctorado en Ingeniería - Ingeniería Mecánica y Mecatrónicaspa
dc.relation.indexedBiremespa
dc.relation.referencesAbedin, Monika, and Nicole King. 2010. “Diverse Evolutionary Paths to Cell Adhesion.” Trends in Cell Biology 20 (December): 734–42. https://doi.org/10.1016/j.tcb.2010.08.002.spa
dc.relation.referencesAcharya, B. R., and A. S. Yap. 2016. “Cell-Cell Adhesion and the Cytoskeleton.” In Encyclopedia of Cell Biology, edited by Ralph A. Bradshaw and Philip D. Stahl, 704–12. Waltham: Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-12-394447- 4.20068-0.spa
dc.relation.referencesAchilli, Matteo, and Diego Mantovani. 2010. “Tailoring Mechanical Properties of Collagen– Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation.” Polymers 2: 664–80. https://doi.org/10.3390/polym2040664.spa
dc.relation.referencesAhmadiankia, Naghmeh. 2020. “In Vitro and in Vivo Studies of Cancer Cell Behavior Under Nutrient Deprivation.” Cell Biology International 44 (August): 1588–97. https://doi.org/10.1002/cbin.11368spa
dc.relation.referencesAkisaka, Toshitaka, Hisaho Yoshida, Reiko Suzuki, and Keiko Takama. 2008. “Adhesion Structures and Their Cytoskeleton-Membrane Interactions at Podosomes of Osteoclasts in Culture.” Cell and Tissue Research 331 (March): 625–41. https://doi.org/10.1007/s00441- 007-0552-x.spa
dc.relation.referencesAkram, M. 2013. “Mini-Review on Glycolysis and Cancer.” Journal of Cancer Education 28: 454–57. https://doi.org/10.1007/s13187-013-0486-9.spa
dc.relation.referencesAlabduladhem, TO, and B Bordoni. 2023. “Physiology, Krebs Cycle. [Updated 2022 Nov 23].” StatPearls [Internet]. https://www.ncbi.nlm.nih.gov/books/NBK556032/.spa
dc.relation.referencesAlberts, B, A Johnson, and J Lewis. 2002. “Chapter 11, the Cytoskeleton and Cell Movement.” In Molecular Biology of the Cell. 4th Edition. Garland science.spa
dc.relation.referencesAlbu, MG, I Titorencu, and MV Ghica. 2011. “Collagen-Based Drug Delivery Systems for Tissue Engineering. In: Pignatello, r.(ed.) Biomaterials Applications for Nanomedicine.” Intech Open Acces Publisher, Rijeka 339spa
dc.relation.referencesAllaire, Joannie M., Shauna M. Crowley, Hong T. Law, Sun-Young Chang, Hyun-Jeong Ko, and Bruce A. Vallance. 2018. “The Intestinal Epithelium: Central Coordinator of Mucosal Immunity.” Trends in Immunology. https://doi.org/https://doi.org/10.1016/j.it.2018.04.002.spa
dc.relation.referencesAltea-Manzano, Patricia, Alejandro M Cuadros, Lindsay A Broadfield, and Sarah-Maria Fendt. 2020. “Nutrient Metabolism and Cancer in the in Vivo Context: A Metabolic Game of Give and Take.” EMBO Reports 21 (October). https://doi.org/10.15252/embr.202050635.spa
dc.relation.referencesAltman, Brian J., Zachary E. Stine, and Chi V. Dang. 2016. “From Krebs to Clinic: Glutamine Metabolism to Cancer Therapy.” Nature Reviews Cancer 16 (October): 619–34. https://doi.org/10.1038/nrc.2016.71.spa
dc.relation.referencesAlvarez, Yanina, and Michael Smutny. 2022. “Emerging Role of Mechanical Forces in Cell Fate Acquisition.” Frontiers in Cell and Developmental Biology 10 (May). https://doi.org/10.3389/fcell.2022.864522spa
dc.relation.referencesAlves, Samara Rodrigues, Italo Rodrigo Calori, Hong Bi, and Antonio Claudio Tedesco. 2023. “Characterization of Glioblastoma Spheroid Models for Drug Screening and Phototherapy Assays.” OpenNano 9 (January): 100116. https://doi.org/10.1016/j.onano.2022.100116spa
dc.relation.referencesAmado, Nathália G., Danilo Predes, Marcela M. Moreno, Igor O. Carvalho, Fábio A. Mendes, and José G. Abreu. 2014. “Flavonoids and Wnt/β-Catenin Signaling: Potential Role in Colorectal Cancer Therapies.” International Journal of Molecular Sciences 15 (7): 12094– 106. https://doi.org/10.3390/ijms150712094spa
dc.relation.referencesAnguiano, María, Carlos Castilla, Martin Maska, Cristina Ederra, Rafael Peláez, Xabier Morales, Gorka Muñoz–Arrieta, et al. 2017. “Characterization of Three–Dimensional Cancer Cell Migration in Mixed Collagen–Matrigel Scaffolds Using Microfluidics and Image Analysis.” PLoS ONE 12. https://doi.org/10.1371/journal.pone.0171417.spa
dc.relation.referencesAntal, M. J. J. 1982. “Biomass Pyrolysis: A Review of the Literature Part 1 - Carbohydrate Pyrolysis.” In Advances in Solar Energy Vol. 1 American Solar Energy Society, 61–111spa
dc.relation.referencesAntoine, Elizabeth E., Pavlos P. Vlachos, and Marissa Nichole Rylander. 2014. “Review of Collagen i Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport.” Tissue Engineering – Part B: Reviews 20: 683–96. https://doi.org/10.1089/ten.teb.2014.0086.spa
dc.relation.referencesAntone, Paolo Dell’. 2012. “Energy Metabolism in Cancer Cells: How to Explain the Warburg and Crabtree Effects?” Medical Hypotheses 79 (September): 388–92. https://doi.org/10.1016/j.mehy.2012.06.002spa
dc.relation.referencesArevalo, Richard C., Jeffrey S. Urbach, and Daniel L. Blair. 2010. “Size–Dependent Rheology of Type–i Collagen Networks.” Biophysical Journal 99: L65–67. https://doi.org/10.1016/j.bpj.2010.08.008spa
dc.relation.referencesArgatov, Ivan, Alexei Iantchenko, and Vitaly Kocherbitov. 2017. “How to Define the Storage and Loss Moduli for a Rheologically Nonlinear Material?: A Constructive Review of Nonlinear Rheological Measures.” Continuum Mechanics and Thermodynamics 29: 1375–87. https://doi.org/10.1007/s00161--017--0584--8.spa
dc.relation.referencesAugestad, K. M., P. M. Bakaki, J. Rose, B. P. Crawshaw, R. O. Lindsetmo, L. M. Dørum, S. M. Koroukian, and C. P. Delaney. 2015. “Metastatic spread pattern after curative colorectal cancer surgery. A retrospective, longitudinal analysis.” Cancer Epidemiology 39 (5): 734– 44. https://doi.org/10.1016/j.canep.2015.07.009.spa
dc.relation.referencesAvendano, Alex, Jonathan J. Chang, Marcos G. Cortes-Medina, Aaron J. Seibel, Bitania R. Admasu, Cassandra M. Boutelle, Andrew R. Bushman, et al. 2020. “Integrated Biophysical Characterization of Fibrillar Collagen-Based Hydrogels.” ACS Biomaterials Science & Engineering 6 (March): 1408–17. https://doi.org/10.1021/acsbiomaterials.9b01873spa
dc.relation.referencesAyuso, Jose M., Haneen A. Basheer, Rosa Monge, Pablo Sánchez-Álvarez, Manuel Doblaré, Steven D. Shnyder, Victoria Vinader, Kamyar Afarinkia, Luis J. Fernández, and Ignacio Ochoa. 2015. “Study of the chemotactic response of multicellular spheroids in a microfluidic device.” PLoS ONE 10 (10): 1–16. https://doi.org/10.1371/journal.pone.0139515spa
dc.relation.referencesAyuso, Jose M, Rosa Monge, Alicia Martínez–González, María Virumbrales–Muñoz, Guillermo A Llamazares, Javier Berganzo, Aurelio Hernández–Laín, et al. 2017. “Glioblastoma on a Microfluidic Chip: Generating Pseudopalisades and Enhancing Aggressiveness Through Blood Vessel Obstruction Events.” Neuro–Oncology 19: 503–13. https://doi.org/10.1093/neuonc/now230.spa
dc.relation.referencesAzizipour, Neda, Rahi Avazpour, Mohamad Sawan, Derek H. Rosenzweig, and Abdellah Ajji. 2022. “Uniformity of Spheroids-on-a-Chip by Surface Treatment of PDMS Microfluidic Platforms.” Sensors & Diagnostics 1: 750–64. https://doi.org/10.1039/D2SD00004Kspa
dc.relation.referencesBailey, Christina E, Chung-Yuan Hu, Y Nancy You, Brian K Bednarski, Miguel A RodriguezBigas, John M Skibber, Scott B Cantor, and George J Chang. 2015. “Increasing Disparities in the Age-Related Incidences of Colon and Rectal Cancers in the United States, 1975-2010.” JAMA Surgery 150 (1): 17–22spa
dc.relation.referencesBaker, Brendon M., and Christopher S. Chen. 2012. “Deconstructing the Third Dimension – How 3D Culture Microenvironments Alter Cellular Cues.” Journal of Cell Science, January. https://doi.org/10.1242/jcs.079509.spa
dc.relation.referencesBalcioglu, Hayri E, Bob van de Water, and Erik H J Danen. 2016. “Tumor–Induced Remote ECM Network Orientation Steers Angiogenesis.” Scientific Reports 6: 22580. https://doi.org/10.1038/srep22580spa
dc.relation.referencesBarisam, Maryam, Mohammad Said Saidi, Navid Kashaninejad, and Nam-Trung Nguyen. 2018. “Prediction of Necrotic Core and Hypoxic Zone of Multicellular Spheroids in a Microbioreactor with a u-Shaped Barrier.” Micromachines 9 (February). https://doi.org/10.3390/mi9030094.spa
dc.relation.referencesBarton, Bruce A. 1996. “Chapter 2 - General Biology of Salmonids.” In Principles of Salmonid Culture, edited by William Pennell and Bruce A. Barton, 29:29–95. Developments in Aquaculture and Fisheries Science. Elsevier. https://doi.org/https://doi.org/10.1016/S0167-9309(96)80005-6.spa
dc.relation.referencesBeco, Simon de, François Amblard, and Sylvie Coscoy. 2012. “New Insights into the Regulation of e-Cadherin Distribution by Endocytosis,” 63–108. https://doi.org/10.1016/B978-0-12-394306-4.00008-3.spa
dc.relation.referencesBelly, Henry De, Aki Stubb, Ayaka Yanagida, Céline Labouesse, Philip H. Jones, Ewa K. Paluch, and Kevin J. Chalut. 2021. “Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate.” Cell Stem Cell 28 (February): 273–284.e6. https://doi.org/10.1016/j.stem.2020.10.018.spa
dc.relation.referencesBergert, Martin, Sergio Lembo, Sumana Sharma, Luigi Russo, Danica Milovanovic, Kristjan H. Gretarsson, Mandy Bormel, et al. 2021. “Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.” Cell Stem Cell 28 (February): 209–216.e4. https://doi.org/10.1016/j.stem.2020.10.017spa
dc.relation.referencesBiancur, Douglas E., and Alec C. Kimmelman. 2018. “The Plasticity of Pancreatic Cancer Metabolism in Tumor Progression and Therapeutic Resistance.” Biochimica Et Biophysica Acta (BBA) - Reviews on Cancer 1870 (August): 67–75. https://doi.org/10.1016/j.bbcan.2018.04.011.spa
dc.relation.referencesBoj, Sylvia F, Annelotte M Vonk, Marvin Statia, Jinyi Su, Robert R G Vries, Jeffrey M Beekman, and Hans Clevers. 2017. “Forskolin-Induced Swelling in Intestinal Organoids: An in Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients.” Journal of Visualized Experiments : JoVE, February. https://doi.org/10.3791/55159.spa
dc.relation.referencesBordeleau, Francois, Turi A. Alcoser, and Cynthia A. Reinhart-King. 2014. “Physical Biology in Cancer. 5. The Rocky Road of Metastasis: The Role of Cytoskeletal Mechanics in Cell Migratory Response to 3D Matrix Topography.” American Journal of Physiology-Cell Physiology 306 (January): C110–20. https://doi.org/10.1152/ajpcell.00283.2013.spa
dc.relation.referencesBotteri, Edoardo, Simona Iodice, Vincenzo Bagnardi, Sara Raimondi, Albert B Lowenfels, and Patrick Maisonneuve. 2008. “Smoking and Colorectal Cancer: A Meta-Analysis.” Jama 300 (23): 2765–78spa
dc.relation.referencesBrat, Daniel J, Balveen Kaur, and Erwin G Van Meir. 2003. “Genetic Modulation of Hypoxia Induced Gene Expression and Angiogenesis: Relevance to Brain Tumors.” Frontiers in Bioscience : A Journal and Virtual Library 8 (January): d100–16. https://doi.org/10.2741/942spa
dc.relation.referencesBrauchle, Eva, Jana Kasper, Ruben Daum, Nicolas Schierbaum, Claudius Falch, Andreas Kirschniak, Tilman E Schaffer, and Katja Schenke–Layland. 2018. “Biomechanical and Biomolecular Characterization of Extracellular Matrix Structures in Human Colon Carcinomas.” Matrix Biology 68–69: 180–93. https://doi.org/https://doi.org/10.1016/j.matbio.2018.03.016.spa
dc.relation.referencesBreslin, Susan, and Lorraine O’Driscoll. 2013. “Three-Dimensional Cell Culture: The Missing Link in Drug Discovery.” Drug Discovery Today 18 (March): 240–49. https://doi.org/10.1016/j.drudis.2012.10.003.spa
dc.relation.referencesBrierley, James D, Mary K Gospodarowicz, and Christian Wittekind. 2016. TNM Classification of Malignant Tumours, 8th Edition. Wiley-Blackwellspa
dc.relation.referencesBrown, Rachel E., Sarah P. Short, and Christopher S. Williams. 2018. “Colorectal Cancer and Metabolism.” Current Colorectal Cancer Reports 14 (December): 226–41. https://doi.org/10.1007/s11888-018-0420-yspa
dc.relation.referencesBrunner, Joël, Sakthikumar Ragupathy, and Gerrit Borchard. 2021. “Target Specific Tight Junction Modulators.” Advanced Drug Delivery Reviews 171: 266–88. https://doi.org/https://doi.org/10.1016/j.addr.2021.02.008spa
dc.relation.referencesBurgess, David R. 1975. “Morphogenesis of Intestinal Villi.” Development 34 (3): 723–40. http://dev.biologists.org/content/34/3/723.spa
dc.relation.referencesBurgess, David R 1976. “Structure of the Epithelial-Mesenchymal Interface During Early Morphogenesis of the Chick Duodenum.” Tissue and Cell 8 (1): 147–58. https://doi.org/https://doi.org/10.1016/0040-8166(76)90026-4spa
dc.relation.referencesBurleson, Kathryn M, Linda K Hansen, and Amy P N Skubitz. 2004. “Ovarian Carcinoma Spheroids Disaggregate on Type i Collagen and Invade Live Human Mesothelial Cell Monolayers.” Clinical & Experimental Metastasis 21: 685–97. https://doi.org/10.1007/s10585-004-5768-5spa
dc.relation.references“Can You Live Without Your Large Intestine? Small Intestine?” n.d. https://www.manhattangastroenterology.com/can-you-live-without-your-large-intestinesmall-intestine/spa
dc.relation.referencesCarey, Shawn P., Timothy M. D’Alfonso, Sandra J. Shin, and Cynthia A. Reinhart-King. 2012. “Mechanobiology of Tumor Invasion: Engineering Meets Oncology.” Critical Reviews in Oncology/Hematology 83 (August): 170–83. https://doi.org/10.1016/j.critrevonc.2011.11.005spa
dc.relation.referencesCatalano, Veronica, Alice Turdo, Simone Di Franco, Francesco Dieli, Matilde Todaro, and Giorgio Stassi. 2013. “Tumor and its microenvironment: A synergistic interplay.” Seminars in Cancer Biology 23 (6 PB): 522–32. https://doi.org/10.1016/j.semcancer.2013.08.007spa
dc.relation.referencesCattin, Sarah, Laurent Ramont, and Curzio Ruegg. 2018. “Characterization and in Vivo Validation of a Three–Dimensional Multi–Cellular Culture Model to Study Heterotypic Interactions in Colorectal Cancer Cell Growth, Invasion and Metastasis.” Frontiers in Bioengineering and Biotechnology 6: 1–14. https://doi.org/10.3389/fbioe.2018.00097.spa
dc.relation.references“Cell Energy and Cell Functions.” n.d. https://www.nature.com/scitable/topicpage/cellenergy-and-cell-functions-14024533/.spa
dc.relation.referencesChanet, Soline, and Adam C. Martin. 2014. “Mechanical Force Sensing in Tissues.” In, 317– 52. https://doi.org/10.1016/B978-0-12-394624-9.00013-0.spa
dc.relation.referencesCharoen, Kristie M, Brian Fallica, Yolonda L Colson, Muhammad H Zaman, and Mark W Grinstaff. 2014. “Embedded Multicellular Spheroids as a Biomimetic 3D Cancer Model for Evaluating Drug and Drug–Device Combinations.” Biomaterials 35 (February): 2264–71. https://doi.org/10.1016/j.biomaterials.2013.11.038spa
dc.relation.referencesChen, Hong–Chen. 2005. “Boyden Chamber Assay.” Methods in Molecular Biology (Clifton, N.J.) 294: 15–22. https://doi.org/10.1385/1--59259--860--9:015.spa
dc.relation.referencesChin, Alana M., David R. Hill, Megan Aurora, and Jason R. Spence. 2017. “Morphogenesis and Maturation of the Embryonic and Postnatal Intestine.” Seminars in Cell & Developmental Biology 66: 81–93. https://doi.org/https://doi.org/10.1016/j.semcdb.2017.01.011spa
dc.relation.referencesChiodi, Ilaria, Giulia Picco, Carolina Martino, and Chiara Mondello. 2019. “Cellular Response to Glutamine and/or Glucose Deprivation in in Vitro Transformed Human Fibroblasts.” Oncology Reports, April. https://doi.org/10.3892/or.2019.7125spa
dc.relation.referencesChoo, Yeon Woong, Juhee Jeong, and Keehoon Jung. 2020. “Recent Advances in Intravital Microscopy for Investigation of Dynamic Cellular Behavior in Vivo.” BMB Reports 53 (July): 357–66. https://doi.org/10.5483/BMBRep.2020.53.7.069spa
dc.relation.referencesCiccone, Giuseppe, Oana Dobre, Graham M. Gibson, Jose Manuel Rey, Cristina Gonzalez Garcia, Massimo Vassalli, Manuel Salmeron Sanchez, and Manlio Tassieri. 2020. “What Caging Force Cells Feel in 3D Hydrogels: A Rheological Perspective.” Advanced Healthcare Materials 9: 1–6. https://doi.org/10.1002/adhm.202000517.spa
dc.relation.referencesClark, Wallace H., Margaret A. Tucker, and Alisa M. Goldstein. 1995. “Parenchymal-stromal interactions in neoplasia: Theoretical considerations and observations in melanocytic neoplasia.” Acta Oncologica 34 (6): 749–57. https://doi.org/10.3109/02841869509127182.spa
dc.relation.referencesClevers, Hans. 2013a. “Review The Intestinal Crypt , A Prototype Stem Cell Compartment.” Cell 154 (2): 274–84. https://doi.org/10.1016/j.cell.2013.07.004spa
dc.relation.references———. 2013b. “The Intestinal Crypt, a Prototype Stem Cell Compartment.” Cell 154 (2): 274–84. https://doi.org/https://doi.org/10.1016/j.cell.2013.07.004.spa
dc.relation.references———. 2013c. “The Intestinal Crypt, a Prototype Stem Cell Compartment.” Cell 154 (2): 274–84. https://doi.org/10.1016/j.cell.2013.07.004.spa
dc.relation.referencesCnop, Miriam, Fabienne Foufelle, and Licio A. Velloso. 2012. “Endoplasmic Reticulum Stress, Obesity and Diabetes.” Trends in Molecular Medicine 18 (January): 59–68. https://doi.org/10.1016/j.molmed.2011.07.010.spa
dc.relation.references“COLON - NORMAL HISTOLOGY.” n.d. https://medicine.nus.edu.sg/pathweb/normalhistology/colon/spa
dc.relation.references“Colorectal Cancer.” n.d. https://www.rhythmbio.com/colorectal-cancer/spa
dc.relation.references“Colorrectal Cancer: Stages.” n.d. https://www.cancer.net/cancer-types/colorectalcancer/stagesspa
dc.relation.referencesCooper, GM. 2000a. “Actin, Myosin, and Cell Movement.” In The Cell: A Molecular Approach. 2nd Edition. Sunderland (MA): Sinauer Associatesspa
dc.relation.references———. 2000b. “Chapter 11, the Cytoskeleton and Cell Movement.” In The Cell: A Molecular Approach. 2nd Edition. Sunderland (MA): Sinauer Associatesspa
dc.relation.references———. 2000c. “Microtubules.” In The Cell: A Molecular Approach. 2nd Edition. Sunderland (MA): Sinauer Associatesspa
dc.relation.references———. 2000d. “Structure and Organization of Actin Filaments.” In The Cell: A Molecular Approach. 2nd Edition. Sunderland (MA): Sinauer Associatesspa
dc.relation.referencesCosta, Erico Tosoni, and Anamaria Aranha Camargo. 2018. “Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains Using Tri–Dimensional (3D) Spheroid Invasion Assay.” Methods in Molecular Biology (Clifton, N.J.) 1731: 155–68. https://doi.org/10.1007/978--1--4939--7595--2_15spa
dc.relation.referencesCruz, Nelly M, Xuewen Song, Stefan M Czerniecki, Ramila E Gulieva, Angela J Churchill, Yong Kyun Kim, Kosuke Winston, et al. 2017. “Organoid Cystogenesis Reveals a Critical Role of Microenvironment in Human Polycystic Kidney Disease.” Nature Materials 16 (November): 1112–19. https://doi.org/10.1038/nmat4994.spa
dc.relation.referencesCui, X., Y. Hartanto, and H. Zhang. 2017. “Advances in Multicellular Spheroids Formation.” Journal of The Royal Society Interface 14 (February): 20160877. https://doi.org/10.1098/rsif.2016.0877spa
dc.relation.referencesCzajka, Caitlin A., Agnes Nagy Mehesz, Thomas C. Trusk, Michael J. Yost, and Christopher J. Drake. 2014. “Scaffold-Free Tissue Engineering: Organization of the Tissue Cytoskeleton and Its Effects on Tissue Shape.” Annals of Biomedical Engineering 42 (May): 1049–61. https://doi.org/10.1007/s10439-014-0986-8.spa
dc.relation.referencesDai, Chunyan, Feifei Sun, Chunpeng Zhu, and Xun Hu. 2013. “Tumor Environmental Factors Glucose Deprivation and Lactic Acidosis Induce Mitotic Chromosomal Instability - an Implication in Aneuploid Human Tumors.” PLoS ONE 8 (May): e63054. https://doi.org/10.1371/journal.pone.0063054.spa
dc.relation.referencesDaster, Silvio, Nunzia Amatruda, Diego Calabrese, Robert Ivanek, Eleonora Turrini, Raoul A. Droeser, Paul Zajac, et al. 2017. “Induction of Hypoxia and Necrosis in Multicellular Tumor Spheroids Is Associated with Resistance to Chemotherapy Treatment.” Oncotarget 8 (January): 1725–36. https://doi.org/10.18632/oncotarget.13857spa
dc.relation.referencesDauca, M, F Bouziges, S Colin, M Kedinger, MK Keller, J Schilt, P Simon-Assmann, and K Haffen. 1990. “Development of the Vertebrate Small Intestine and Mechanisms of Cell Differentiation.” The International Journal of Developmental Biology 34: 205–18spa
dc.relation.referencesDe Wever, Olivier, Pieter Demetter, Marc Mareel, and Marc Bracke. 2008. “Stromal myofibroblasts are drivers of invasive cancer growth.” International Journal of Cancer 123 (10): 2229–38. https://doi.org/10.1002/ijc.23925spa
dc.relation.referencesDe Wever, Olivier, and Marc Mareel. 2003. “Role of tissue stroma in cancer cell invasion.” Journal of Pathology 200 (4): 429–47. https://doi.org/10.1002/path.1398spa
dc.relation.referencesDelva, Emmanuella, Dana K Tucker, and Andrew P Kowalczyk. 2009. “The Desmosome.” Cold Spring Harbor Perspectives in Biology 1 (August): a002543. https://doi.org/10.1101/cshperspect.a002543spa
dc.relation.referencesDemeter, Maria, Ion Calina, Anca Scarisoreanu, Marin Micutz, and Madalina Albu Kaya. 2022. “Correlations on the Structure and Properties of Collagen Hydrogels Produced by eBeam Crosslinking.” Materials 15 (October): 7663. https://doi.org/10.3390/ma15217663.spa
dc.relation.referencesDenoix, P. F. 1946. “Enquete permanent dans les centres anticancereux.” Bull. Inst. Nat. Hyg. 1: 70+spa
dc.relation.referencesDerycke, Lara, Christophe Stove, Anne-Sophie Vercoutter-Edouart, Olivier De Wever, Laurent Dollé, Nathalie Colpaert, Herman Depypere, Jean-Claude Michalski, and Marc Bracke. 2011. “The Role of Non-Muscle Myosin IIA in Aggregation and Invasion of Human MCF-7 Breast Cancer Cells.” The International Journal of Developmental Biology 55: 835–40. https://doi.org/10.1387/ijdb.113336ldspa
dc.relation.referencesDeshpande, OA, and SS Mohiuddin. 2023. “Biochemistry, Oxidative Phosphorylation. [Updated 2023 Jul 31].” StatPearls [Internet]. https://www.ncbi.nlm.nih.gov/books/NBK553192/.spa
dc.relation.referencesDevanny, Alexander J., Michelle B. Vancura, and Laura J. Kaufman. 2021. “Exploiting Differential Effects of Actomyosin Contractility to Control Cell Sorting Among Breast Cancer Cells.” Molecular Biology of the Cell 32 (November). https://doi.org/10.1091/mbc.E21-07- 0357spa
dc.relation.referencesDevarasetty, Mahesh, Aleksander Skardal, Kyle Cowdrick, Frank Marini, and Shay Soker. 2017. “Bioengineered Submucosal Organoids for in Vitro Modeling of Colorectal Cancer.” Tissue Engineering Part A 23 (October): 1026–41. https://doi.org/10.1089/ten.tea.2017.0397.spa
dc.relation.referencesDevenport, Samantha N, Rashi Singhal, Megan D Radyk, Joseph G Taranto, Samuel A Kerk, Brandon Chen, Joshua W Goyert, et al. 2021. “Colorectal Cancer Cells Utilize Autophagy to Maintain Mitochondrial Metabolism for Cell Proliferation Under Nutrient Stress.” JCI Insight 6 (June). https://doi.org/10.1172/jci.insight.138835.spa
dc.relation.referencesDischer, Dennis E, Paul Janmey, and Yu-Li Wang. 2005. “Tissue Cells Feel and Respond to the Stiffness of Their Substrate.” Science (New York, N.Y.) 310 (November): 1139–43. https://doi.org/10.1126/science.1116995.spa
dc.relation.referencesDolznig, Helmut, Christian Rupp, Christina Puri, Christian Haslinger, Norbert Schweifer, Elisabeth Wieser, Dontscho Kerjaschki, and Pilar Garin-Chesa. 2011. “Modeling Colon Adenocarcinomas in Vitro: A 3D Co-Culture System Induces Cancer-Relevant Pathways Upon Tumor Cell and Stromal Fibroblast Interaction.” American Journal of Pathology 179: 487–501. https://doi.org/10.1016/j.ajpath.2011.03.015.spa
dc.relation.referencesDoukas, J, A H Cutler, C A Boswell, I Joris, and G Maino. 1994. “Reversible Endothelial Cell Relaxation Induced by Oxygen and Glucose Deprivation. A Model of Ischemia in Vitro.” The American Journal of Pathology 145 (July): 211–19spa
dc.relation.referencesDrake, A, Richard, Wayne Vogl, and Adam WM Mitchell. 2014. Gray’s Anatomy for Students e-Book. Churchill Livingstonespa
dc.relation.referencesDruzhkova, Irina, Marina Shirmanova, Nadezhda Ignatova, Varvara Dudenkova, Maria Lukina, Elena Zagaynova, Dina Safina, et al. 2020. “Expression of EMT-Related Genes in Hybrid e/m Colorectal Cancer Cells Determines Fibroblast Activation and Collagen Remodeling.” International Journal of Molecular Sciences 21 (October): 8119. https://doi.org/10.3390/ijms21218119spa
dc.relation.referencesDucker, Martin, Valerie Millar, Daniel Ebner, and Francis G Szele. 2020. “A Semi–Automated and Scalable 3D Spheroid Assay to Study Neuroblast Migration.” Stem Cell Reports 15: 789– 802. https://doi.org/https://doi.org/10.1016/j.stemcr.2020.07.012spa
dc.relation.referencesDwyer, Amy R, Lesley G Ellies, Andrea L Holme, and Fiona J Pixley. 2016. “A Three– Dimensional Co–Culture System to Investigate Macrophage–Dependent Tumor Cell Invasion.” Journal of Biological Methods 3: e49. https://doi.org/10.14440/jbm.2016.132spa
dc.relation.referencesEdwards, Richard J., Adrian T. Saurin, Roby D. Rakhit, and Michael S. Marber. 2000. “Therapeutic Potential of Ischaemic Preconditioning.” British Journal of Clinical Pharmacology 50 (August): 87–97. https://doi.org/10.1046/j.1365-2125.2000.00236.xspa
dc.relation.referencesEkblom, Peter, Dietmar Vestweber, and Rolf Kemler. 1986. “Cell-Matrix Interactions and Cell Adhesion During Development.” Annual Review of Cell Biology 2 (1): 27–47. https://doi.org/10.1146/annurev.cb.02.110186.000331spa
dc.relation.referencesEsmaeilniakooshkghazi, Amin, Eric Pham, Sudeep P. George, Afzal Ahrorov, Fabian R. Villagomez, Michael Byington, Srijita Mukhopadhyay, et al. 2023. “In Colon Cancer Cells Fascin1 Regulates Adherens Junction Remodeling.” The FASEB Journal 37 (March). https://doi.org/10.1096/fj.202201454R.spa
dc.relation.referencesEtienne-Manneville, Sandrine. 2018. “Cytoplasmic Intermediate Filaments in Cell Biology.” Annual Review of Cell and Developmental Biology 34 (October): 1–28. https://doi.org/10.1146/annurev-cellbio-100617-062534.spa
dc.relation.referencesFang, Chih-Yeu, Chung-Chun Wu, Chia-Lang Fang, Wei-Yu Chen, and Chi-Long Chen. 2017. “Long-Term Growth Comparison Studies of FBS and FBS Alternatives in Six Head and Neck Cell Lines.” PLOS ONE 12 (June): e0178960. https://doi.org/10.1371/journal.pone.0178960spa
dc.relation.referencesFerraro, Rosalia, Flora Ascione, Prashant Dogra, Vittorio Cristini, Stefano Guido, and Sergio Caserta. 2022. “Diffusion-Induced Anisotropic Cancer Invasion: A Novel Experimental Method Based on Tumor Spheroids.” AIChE Journal 68 (July). https://doi.org/10.1002/aic.17678spa
dc.relation.referencesFidler, Isaiah J. 2003. “The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited.” https://doi.org/10.1038/nrc1098.spa
dc.relation.referencesFlam, Josipa. 2017. “High Tumor Stroma Proportion Is a Worse Prognostic Factor in Colorectal Cancer.” ACTA CLINICA CROATICA, 73–79. https://doi.org/10.20471/acc.2017.56.01.11spa
dc.relation.referencesFouad, MA, AM Agha, MM Al Merzabani, and SA Shouman. 2013. “Resveratrol Inhibits Proliferation, Angiogenesis and Induces Apoptosis in Colon Cancer Cells.” Human & Experimental Toxicology 32 (October): 1067–80. https://doi.org/10.1177/0960327113475679.spa
dc.relation.referencesFradette, Katherine, H. J. Keselman, James Algina, Lisa Lix, and Rand R. Wilcox. 2003. “Conventional and Robust Paired and Independent-Samples t Tests: Type i Error and Power Rates.” Journal of Modern Applied Statistical Methods 2: 481–96. https://doi.org/10.22237/jmasm/1067646120.spa
dc.relation.referencesFraley, Stephanie I., Pei-hsun Wu, Lijuan He, Yunfeng Feng, Ranjini Krisnamurthy, Gregory D. Longmore, and Denis Wirtz. 2015. “Three-Dimensional Matrix Fiber Alignment Modulates Cell Migration and MT1-MMP Utility by Spatially and Temporally Directing Protrusions.” Scientific Reports 5 (October): 14580. https://doi.org/10.1038/srep14580spa
dc.relation.referencesFranke, G. 1994. Nutzpflanzen Der Tropen Und Subtropen. Stuttgart: UTB.spa
dc.relation.referencesFriedl, Peter, and Stephanie Alexander. 2011. “Cancer Invasion and the Microenvironment: Plasticity and Reciprocity.” Cell 147: 992–1009. https://doi.org/10.1016/j.cell.2011.11.016.spa
dc.relation.referencesFriedl, Peter, and Darren Gilmour. 2009. “Collective cell migration in morphogenesis, regeneration and cancer.” Nature Reviews Molecular Cell Biology 10 (7): 445–57. https://doi.org/10.1038/nrm2720spa
dc.relation.referencesFriedl, Peter, and Katarina Wolf. 2003. “Tumour–Cell Invasion and Migration: Diversity and Escape Mechanisms.” Nature Reviews Cancer 3: 362–74. https://doi.org/10.1038/nrc1075.spa
dc.relation.references———. 2010. “Plasticity of Cell Migration: A Multiscale Tuning Model.” Journal of Cell Biology 188: 11–19. https://doi.org/10.1083/jcb.200909003spa
dc.relation.referencesFriedrich, Juergen, Reinhard Ebner, and Prof. Dr Leoni A. Kunz-Schughart. 2007. “Experimental Anti-Tumor Therapy in 3-d: Spheroids-Old Hat or New Challenge?” International Journal of Radiation Biology 83 (11-12): 849–71. https://doi.org/10.1080/09553000701727531spa
dc.relation.referencesGao, Nan, Peter White, and Klaus H Kaestner. 2009. “Establishment of Intestinal Identity and Epithelial-Mesenchymal Signaling by Cdx2.” Developmental Cell 16 (4): 588–99. https://doi.org/10.1016/j.devcel.2009.02.010.spa
dc.relation.referencesGarvin, Kelley A., Jacob VanderBurgh, Denise C. Hocking, and Diane Dalecki. 2013. “Controlling Collagen Fiber Microstructure in Three–Dimensional Hydrogels Using Ultrasound.” The Journal of the Acoustical Society of America 134 (August): 1491–1502. https://doi.org/10.1121/1.4812868spa
dc.relation.references“Gastrointestinal Wall.” n.d. https://en.wikipedia.org/wiki/Gastrointestinal_wall.spa
dc.relation.referencesGavara, Núria. 2017. “A Beginner’s Guide to Atomic Force Microscopy Probing for Cell Mechanics.” Microscopy Research and Technique 80: 75–84. https://doi.org/10.1002/jemt.22776spa
dc.relation.referencesGayan, Sukanya, Abhishek Teli, and Tuli Dey. 2017. “Inherent Aggressive Character of Invasive and Non-Invasive Cells Dictates the in Vitro Migration Pattern of Multicellular Spheroid.” Scientific Reports 7 (September): 11527. https://doi.org/10.1038/s41598-017- 10078-7spa
dc.relation.referencesGeiger, Benjamin, Joachim P Spatz, and Alexander D Bershadsky. 2009. “Environmental Sensing Through Focal Adhesions.” Nature Reviews. Molecular Cell Biology 10 (January): 21–33. https://doi.org/10.1038/nrm2593spa
dc.relation.referencesGhasemi, Asghar, and Saleh Zahediasl. 2012. “Normality Tests for Statistical Analysis: A Guide for Non-Statisticians.” International Journal of Endocrinology and Metabolism 10: 486–89. https://doi.org/10.5812/ijem.3505.spa
dc.relation.referencesGheytanchi, Elmira, Marzieh Naseri, Feridoun Karimi-Busheri, Fatemeh Atyabi, Ensie Sadat Mirsharif, Mahmood Bozorgmehr, Roya Ghods, and Zahra Madjd. 2021. “Morphological and Molecular Characteristics of Spheroid Formation in HT-29 and Caco-2 Colorectal Cancer Cell Lines.” Cancer Cell International 21 (April): 204. https://doi.org/10.1186/s12935-021- 01898-9.spa
dc.relation.referencesGoldmann, W. 2002. “MECHANICAL ASPECTS OF CELL SHAPE REGULATION AND SIGNALING.” Cell Biology International 26 (April): 313–17. https://doi.org/10.1006/cbir.2002.0857spa
dc.relation.referencesGomez-Verjan, Juan C., Oscar S. Barrera-Vázquez, Lizbeth García-Velázquez, Rafael SamperTernent, and Pedro Arroyo. 2020. “Epigenetic Variations Due to Nutritional Status in EarlyLife and Its Later Impact on Aging and Disease.” Clinical Genetics 98 (October): 313–21. https://doi.org/10.1111/cge.13748spa
dc.relation.referencesGong, Xiangyu, Jonathan Kulwatno, and K. L. Mills. 2020. “Rapid Fabrication of Collagen Bundles Mimicking Tumor-Associated Collagen Architectures.” Acta Biomaterialia 108: 128–41. https://doi.org/https://doi.org/10.1016/j.actbio.2020.03.019.spa
dc.relation.referencesGong, Xue, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, and Peng Zhao. 2015. “Generation of Multicellular Tumor Spheroids with Microwell-Based Agarose Scaffolds for Drug Testing.” PLOS ONE 10 (June): e0130348. https://doi.org/10.1371/journal.pone.0130348.spa
dc.relation.referencesGonzález-Rodríguez, Patricia, Jens Fullgrabe, and Bertrand Joseph. 2023. “The Hunger Strikes Back: An Epigenetic Memory for Autophagy.” Cell Death & Differentiation 30 (June): 1404–15. https://doi.org/10.1038/s41418-023-01159-4.spa
dc.relation.referencesKim, Eun Mi, Yu Bin Lee, Hayeon Byun, Hyung-kwan Chang, Jungyul Park, and Heungsoo Shin. 2019. “Fabrication of Spheroids with Uniform Size by Self-Assembly of a Micro-Scaled Cell Sheet (μCS): The Effect of Cell Contraction on Spheroid Formation.” ACS Applied Materials & Interfaces 11 (January): 2802–13. https://doi.org/10.1021/acsami.8b18048spa
dc.relation.referencesKim, Jae Hyun, Kyoung Jin Lee, Yoojeong Seo, Ji-Hee Kwon, Jae Pil Yoon, Jo Yeon Kang, Hyun Jung Lee, et al. 2018. “Effects of Metformin on Colorectal Cancer Stem Cells Depend on Alterations in Glutamine Metabolism.” Scientific Reports 8 (January): 409. https://doi.org/10.1038/s41598-017-18762-4.spa
dc.relation.referencesKim, Jihan, Yuansheng Cao, Christopher Eddy, Youyuan Deng, Herbert Levine, Wouter-Jan Rappel, and Bo Sun. 2021. “The Mechanics and Dynamics of Cancer Cells Sensing Noisy 3D Contact Guidance.” Proceedings of the National Academy of Sciences 118 (March). https://doi.org/10.1073/pnas.2024780118spa
dc.relation.referencesKim, So-Hee, and Kwang-Hyun Baek. 2021. “Regulation of Cancer Metabolism by Deubiquitinating Enzymes: The Warburg Effect.” International Journal of Molecular Sciences 22 (June): 6173. https://doi.org/10.3390/ijms22126173.spa
dc.relation.referencesKnights, Alexander J, Alister P W Funnell, Merlin Crossley, and Richard C M Pearson. 2012. “Holding Tight: Cell Junctions and Cancer Spread.” Trends in Cancer Research 8: 61–69spa
dc.relation.referencesKo, Jihoon, Jungho Ahn, Suryong Kim, Younggyun Lee, Jungseub Lee, Dohyun Park, and Noo Li Jeon. 2019. “Tumor Spheroid–on–a–Chip: A Standardized Microfluidic Culture Platform for Investigating Tumor Angiogenesis.” Lab Chip 19: 2822–33. https://doi.org/10.1039/C9LC00140A.spa
dc.relation.referencesKoelzer, Viktor H., Inti Zlobec, and Alessandro Lugli. 2016. “Tumor Budding in Colorectal Cancer-Ready for Diagnostic Practice?” Human Pathology 47 (January): 4–19. https://doi.org/10.1016/j.humpath.2015.08.007.spa
dc.relation.referencesKoenderink, Gijsje H, and Ewa K Paluch. 2018. “Architecture Shapes Contractility in Actomyosin Networks.” Current Opinion in Cell Biology 50 (February): 79–85. https://doi.org/10.1016/j.ceb.2018.01.015.spa
dc.relation.referencesKosheleva, N. V., I. V. Ilina, I. M. Zurina, A. E. Roskova, A. A. Gorkun, A. V. Ovchinnikov, M. B. Agranat, and I. N. Saburina. 2016. “Laser-Based Technique for Controlled Damage of Mesenchymal Cell Spheroids: A First Step in Studying Reparation in Vitro.” Biology Open 5 (July): 993–1000. https://doi.org/10.1242/bio.017145spa
dc.relation.referencesKovács, Mihály, Judit Tóth, Csaba Hetényi, András Málnási-Csizmadia, and James R. Sellers. 2004. “Mechanism of Blebbistatin Inhibition of Myosin II.” Journal of Biological Chemistry 279 (August): 35557–63. https://doi.org/10.1074/jbc.M405319200spa
dc.relation.referencesKozuka, Kenji, Ying He, Samantha Koo-McCoy, Padmapriya Kumaraswamy, Baoming Nie, Karen Shaw, Priscilla Chan, et al. 2017. “Development and Characterization of a Human and Mouse Intestinal Epithelial Cell Monolayer Platform.” Stem Cell Reports 9 (6): 1976–90. https://doi.org/https://doi.org/10.1016/j.stemcr.2017.10.013.spa
dc.relation.referencesKramer, Nina, Angelika Walzl, Christine Unger, Margit Rosner, Georg Krupitza, Markus Hengstschläger, and Helmut Dolznig. 2013. “In Vitro Cell Migration and Invasion Assays.” Mutation Research – Reviews in Mutation Research 752: 10–24. https://doi.org/10.1016/j.mrrev.2012.08.001spa
dc.relation.referencesKumper, Sandra, Faraz K Mardakheh, Afshan McCarthy, Maggie Yeo, Gordon W Stamp, Angela Paul, Jonathan Worboys, et al. 2016. “Rho-Associated Kinase (ROCK) Function Is Essential for Cell Cycle Progression, Senescence and Tumorigenesis.” eLife 5 (January): e12994. https://doi.org/10.7554/eLife.12203spa
dc.relation.referencesLacalle, David, Héctor Alfonso Castro–Abril, Teodora Randelovic, César Domínguez, Jónathan Heras, Eloy Mata, Gadea Mata, Yolanda Méndez, Vico Pascual, and Ignacio Ochoa. 2021. “SpheroidJ: An Open–Source Set of Tools for Spheroid Segmentation.” Computer Methods and Programs in Biomedicine 200: 105837. https://doi.org/https://doi.org/10.1016/j.cmpb.2020.105837.spa
dc.relation.referencesLanghans, Sigrid A. 2018. “Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning.” Frontiers in Pharmacology 9 (January). https://doi.org/10.3389/fphar.2018.00006.spa
dc.relation.referencesLarsson, Susanna C, Nicola Orsini, and Alicja Wolk. 2005. “Diabetes Mellitus and Risk of Colorectal Cancer: A Meta-Analysis.” Journal of the National Cancer Institute 97 (22): 1679– 87spa
dc.relation.referencesLauzier, Annie, Josiann Normandeau-Guimond, Vanessa Vaillancourt-Lavigueur, Vincent Boivin, Martine Charbonneau, Nathalie Rivard, Michelle S. Scott, Claire M. Dubois, and Steve Jean. 2019. “Colorectal Cancer Cells Respond Differentially to Autophagy Inhibition in Vivo.” Scientific Reports 9 (August): 11316. https://doi.org/10.1038/s41598-019-47659-7spa
dc.relation.referencesLecomte, Julie, Anne Masset, Silvia Blacher, Ludovic Maertens, André Gothot, Marie Delgaudine, Françoise Bruyere, et al. 2012. “Bone Marrow–Derived Myofibroblasts Are the Providers of Pro–Invasive Matrix Metalloproteinase 13 in Primary Tumor.” Neoplasia (New York, N.Y.) 14 (October): 943–51. https://doi.org/10.1593/neo.121092spa
dc.relation.referencesLee, Changhan, Lizzia Raffaghello, Sebastian Brandhorst, Fernando M. Safdie, Giovanna Bianchi, Alejandro Martin-Montalvo, Vito Pistoia, et al. 2012. “Fasting Cycles Retard Growth of Tumors and Sensitize a Range of Cancer Cell Types to Chemotherapy.” Science Translational Medicine 4 (March). https://doi.org/10.1126/scitranslmed.3003293spa
dc.relation.referencesLee, Da Young, Seung Yun Lee, Seung Hyeon Yun, Jae Won Jeong, Jae Hyeon Kim, Hyun Woo Kim, Jung Seok Choi, et al. 2022. “Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat.” Food Science of Animal Resources 42 (September): 775–99. https://doi.org/10.5851/kosfa.2022.e46.spa
dc.relation.referencesLee, Heung Man, Shao Chin Lee, Lan He, Alice Pik Shan Kong, Dandan Mao, Yong Hou, Arthur Chi Kong Chung, Gang Xu, Ronald Ching Wan Ma, and Juliana Chung Ngor Chan. 2021. “Legacy Effect of High Glucose on Promoting Survival of HCT116 Colorectal Cancer Cells by Reducing Endoplasmic Reticulum Stress Response.” American Journal of Cancer Research 11: 6004–23spa
dc.relation.referencesLee, Jonathan M., Shoukat Dedhar, Raghu Kalluri, and Erik W. Thompson. 2006. “The epithelial-mesenchymal transition: New insights in signaling, development, and disease.” Journal of Cell Biology 172 (7): 973–81. https://doi.org/10.1083/jcb.200601018. Lee, Jong Min, Da Yeon Park, Letao Yang, Eun–Joong Kim, Christian D Ahrberg, Ki–Bum Lee, and Bong Geun Chung. 2018. “Generation of Uniform–Sized Multicellular Tumor Spheroids Using Hydrogel Microwells for Advanced Drug Screening.” Scientific Reports 8: 17145. https://doi.org/10.1038/s41598--018--35216--7.spa
dc.relation.referencesLee, Kuen Yong, and David J Mooney. 2012. “Alginate: Properties and Biomedical Applications.” Progress in Polymer Science 37: 106–26. https://doi.org/https://doi.org/10.1016/j.progpolymsci.2011.06.003spa
dc.relation.referencesLee, Yu Bin, Eun Mi Kim, Hayeon Byun, Hyung-kwan Chang, Kwanghee Jeong, Zachary M. Aman, Yu Suk Choi, Jungyul Park, and Heungsoo Shin. 2018. “Engineering Spheroids Potentiating Cell-Cell and Cell-ECM Interactions by Self-Assembly of Stem Cell Microlayer.” Biomaterials 165 (May): 105–20. https://doi.org/10.1016/j.biomaterials.2018.02.049.spa
dc.relation.referencesLei, Kin Fong, Hsueh–Peng Tseng, Chia–Yi Lee, and Ngan–Ming Tsang. 2016. “Quantitative Study of Cell Invasion Process Under Extracellular Stimulation of Cytokine in a Microfluidic Device.” Scientific Reports 6: 25557. https://doi.org/10.1038/srep25557spa
dc.relation.referencesLempereur, Sylvain, Elodie Machado, Fabrice Licata, Lilian Buzer, Isabelle Robineau, Julien Hémon, Payel Banerjee, et al. 2020. “ZeBraInspector, a Whole Organism Screening Platform Enabling Volumetric Analysis of Zebrafish Brain White Matter.” bioRxiv. https://doi.org/10.1101/2020.10.26.353656spa
dc.relation.referencesLessey, Lindyann R., Shaiya C. Robinson, Roopali Chaudhary, and Juliet M. Daniel. 2022. “Adherens Junction Proteins on the Move-from the Membrane to the Nucleus in Intestinal Diseases.” Frontiers in Cell and Developmental Biology 10 (October). https://doi.org/10.3389/fcell.2022.998373spa
dc.relation.referencesLeung, Brendan M., Sasha Cai Lesher-Perez, Toshiki Matsuoka, Christopher Moraes, and Shuichi Takayama. 2015. “Media Additives to Promote Spheroid Circularity and Compactness in Hanging Drop Platform.” Biomaterials Science 3: 336–44. https://doi.org/10.1039/C4BM00319Espa
dc.relation.referencesLevental, Kandice R., Hongmei Yu, Laura Kass, Johnathon N. Lakins, Mikala Egeblad, Janine T. Erler, Sheri F. T. Fong, et al. 2009. “Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling.” Cell 139 (5): 891–906. https://doi.org/10.1016/j.cell.2009.10.027spa
dc.relation.referencesLevine, Beth, and Daniel J Klionsky. 2004. “Development by Self-Digestion: Molecular Mechanisms and Biological Functions of Autophagy.” Developmental Cell 6 (April): 463–77. https://doi.org/10.1016/S1534-5807(04)00099-1spa
dc.relation.referencesLevy, Neil. 2012. “The Use of Animal as Models: Ethical Considerations.” International Journal of Stroke 7: 440–42. https://doi.org/10.1111/j.1747--4949.2012.00772.x.spa
dc.relation.referencesLewis-Tuffin, Laura J, Fausto Rodriguez, Caterina Giannini, Bernd Scheithauer, Brian M Necela, Jann N Sarkaria, and Panos Z Anastasiadis. 2010. “Misregulated e-Cadherin Expression Associated with an Aggressive Brain Tumor Phenotype.” PloS One 5 (October): e13665. https://doi.org/10.1371/journal.pone.0013665.spa
dc.relation.referencesLi, Jiaqiu, Ping Song, Liyuan Zhu, Neelum Aziz, Qiyin Zhou, Yulong Zhang, Wenxia Xu, et al. 2017. “Synthetic Lethality of Glutaminolysis Inhibition, Autophagy Inactivation and Asparagine Depletion in Colon Cancer.” Oncotarget 8 (June): 42664–72. https://doi.org/10.18632/oncotarget.16844spa
dc.relation.referencesLi, Zongwei, Yingying Wang, Haili Wu, Lichao Zhang, Peng Yang, and Zhuoyu Li. 2014. “GRP78 Enhances the Glutamine Metabolism to Support Cell Survival from Glucose Deficiency by Modulating the β-Catenin Signaling.” Oncotarget 5 (July): 5369–80. https://doi.org/10.18632/oncotarget.2105.spa
dc.relation.referencesLim, Chwee Teck, Bershadsky Alexander, and Michael P Sheetz. 2010. “Mechanobiology.” Journal of the Royal Society Interface 7 (June): S291–93spa
dc.relation.referencesLin, Xiaoping, Zizheng Xiao, Tao Chen, Steven H. Liang, and Huiqin Guo. 2020. “Glucose Metabolism on Tumor Plasticity, Diagnosis, and Treatment.” Frontiers in Oncology 10 (March). https://doi.org/10.3389/fonc.2020.00317spa
dc.relation.referencesLinder, Stefan. 2007. “The Matrix Corroded: Podosomes and Invadopodia in Extracellular Matrix Degradation.” Trends in Cell Biology 17 (March): 107–17. https://doi.org/10.1016/j.tcb.2007.01.002.spa
dc.relation.referencesLink, Rabea, Kai Weibenbruch, Motomu Tanaka, Martin Bastmeyer, and Ulrich S. Schwarz. 2023. “Cell Shape and Forces in Elastic and Structured Environments: From Single Cells to Organoids.” Advanced Functional Materials, May. https://doi.org/10.1002/adfm.202302145spa
dc.relation.referencesLintz, Marianne, Adam Munoz, and Cynthia A Reinhart-King. 2017. “The Mechanics of Single Cell and Collective Migration of Tumor Cells.” Journal of Biomechanical Engineering 139 (February): 0210051–59. https://doi.org/10.1115/1.4035121.spa
dc.relation.referencesLiptay, M. J., and C. Mascaux. 2004. “TNM Staging.” In Malignant Tumors of the Lung: Evidence-Based Management, edited by Jean-Paul Sculier and Willard A. Fry, 183–88. Berlin, Heidelberg: Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-18698-1_16.spa
dc.relation.referencesLiu, Geyao, Jiayi Li, and Congying Wu. 2022. “Reciprocal Regulation of Actin Filaments and Cellular Metabolism.” European Journal of Cell Biology 101 (September): 151281. https://doi.org/10.1016/j.ejcb.2022.151281.spa
dc.relation.referencesLiu, Hui, Tao Lu, Gert Jan Kremers, Ann L. B. Seynhaeve, and Timo L. M. Ten Hagen. 2020. “A Microcarrier–Based Spheroid 3D Invasion Assay to Monitor Dynamic Cell Movement in Extracellular Matrix.” Biological Procedures Online 22: 1–12. https://doi.org/10.1186/s12575--019--0114--0spa
dc.relation.referencesLiu, Shuai, Wei Yang, Yunlei Li, and Changqing Sun. 2023. “Fetal Bovine Serum, an Important Factor Affecting the Reproducibility of Cell Experiments.” Scientific Reports 13 (February): 1942. https://doi.org/10.1038/s41598-023-29060-7.spa
dc.relation.referencesLL, Azzouz, and Sharma S. 2023. “Physiology, Large Intestine. [Updated 2022 Aug 1].” StatPearls [Internet]. https://www.ncbi.nlm.nih.gov/books/NBK507857/spa
dc.relation.referencesLo, CM, HB Wang, M Dembo, and YL Wang. 2000. “Cell Movement Is Guided by the Rigidity of the Substrate.” Biophysical Journal 79: 144–52. https://doi.org/10.1016/S0006-- 3495(00)76279--5spa
dc.relation.referencesLock, John G, Bernhard Wehrle-Haller, and Staffan Stromblad. 2008. “Cell-Matrix Adhesion Complexes: Master Control Machinery of Cell Migration.” Seminars in Cancer Biology 18 (February): 65–76. https://doi.org/10.1016/j.semcancer.2007.10.001.spa
dc.relation.referencesLongati, Paola, Xiaohui Jia, Johannes Eimer, Annika Wagman, Michael-Robin Witt, Stefan Rehnmark, Caroline Verbeke, Rune Toftgard, Matthias Lohr, and Rainer L Heuchel. 2013. “3D Pancreatic Carcinoma Spheroids Induce a Matrix-Rich, Chemoresistant Phenotype Offering a Better Model for Drug Testing.” BMC Cancer 13 (December): 95. https://doi.org/10.1186/1471-2407-13-95.spa
dc.relation.referencesLongo, Valter D, and Mark P Mattson. 2014. “Fasting: Molecular Mechanisms and Clinical Applications.” Cell Metabolism 19 (February): 181–92. https://doi.org/10.1016/j.cmet.2013.12.008.spa
dc.relation.referencesLópez-lázaro, Miguel. 2015. “The migration ability of stem cells can explain the existence of cancer of unknown primary site . Rethinking metastasis .” Oncoscience 2 (5): 467–75spa
dc.relation.referencesLow, Eric E, Joshua Demb, Lin Liu, Ashley Earles, Ranier Bustamante, Christina D Williams, Dawn Provenzale, et al. 2020. “Risk Factors for Early-Onset Colorectal Cancer.” Gastroenterology 159 (2): 492–501spa
dc.relation.referencesLugli, Alessandro, Richard Kirsch, Yoichi Ajioka, Fred Bosman, Gieri Cathomas, Heather Dawson, Hala El Zimaity, et al. 2017. “Recommendations for Reporting Tumor Budding in Colorectal Cancer Based on the International Tumor Budding Consensus Conference (ITBCC) 2016.” Modern Pathology 30 (September): 1299–1311. https://doi.org/10.1038/modpathol.2017.46.spa
dc.relation.referencesLuo, Ji, Nicole L Solimini, and Stephen J Elledge. 2009. “Review Principles of Cancer Therapy : Oncogene and Non-oncogene Addiction.” Cell, 823–37. https://doi.org/10.1016/j.cell.2009.02.024spa
dc.relation.referencesLuo, Qing, Dongdong Kuang, Bingyu Zhang, and Guanbin Song. 2016. “Cell Stiffness Determined by Atomic Force Microscopy and Its Correlation with Cell Motility.” Biochimica Et Biophysica Acta (BBA) - General Subjects 1860 (9): 1953–60. https://doi.org/https://doi.org/10.1016/j.bbagen.2016.06.010.spa
dc.relation.referencesMaiuri, Paolo, Jean François Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. 2015. “Actin flows mediate a universal coupling between cell speed and cell persistence.” Cell 161 (2): 374–86. https://doi.org/10.1016/j.cell.2015.01.056.spa
dc.relation.referencesMak, Michael, Fabian Spill, Roger D Kamm, and Muhammad H Zaman. 2016. “Single–Cell Migration in Complex Microenvironments: Mechanics and Signaling Dynamics.” Journal of Biomechanical Engineering 138 (February): 21004. https://doi.org/10.1115/1.4032188spa
dc.relation.referencesMalik, Ruchi, Peter I Lelkes, and Edna Cukierman. 2015. “Biomechanical and biochemical remodeling of stromal extracellular matrix in cancer.” Trends in Biotechnology 33 (4): 230– 36. https://doi.org/10.1016/j.tibtech.2015.01.004.spa
dc.relation.referencesManibog, Kristine, Kannan Sankar, Sun-Ae Kim, Yunxiang Zhang, Robert L. Jernigan, and Sanjeevi Sivasankar. 2016. “Molecular Determinants of Cadherin Ideal Bond Formation: Conformation-Dependent Unbinding on a Multidimensional Landscape.” Proceedings of the National Academy of Sciences 113 (September). https://doi.org/10.1073/pnas.1604012113spa
dc.relation.referencesMao, Bin–Hsu, Mai Thi Kim Nguyen, Ming–Jer Tang, Roger D Kamm, and Ting–Yuan Tu. 2022. “The Interface Stiffness and Topographic Feature Dictate Interfacial Invasiveness of Cancer Spheroids.” Biofabrication. http://iopscience.iop.org/article/10.1088/1758-- 5090/acaa00.spa
dc.relation.referencesMarimuthu, M., N. Rousset, A. St-Georges-Robillard, M. A. Lateef, M. Ferland, A.-M. MesMasson, and T. Gervais. 2018. “Multi-Size Spheroid Formation Using Microfluidic Funnels.” Lab on a Chip 18: 304–14. https://doi.org/10.1039/C7LC00970D.spa
dc.relation.referencesMartínez-Reyes, Inmaculada, and Navdeep S. Chandel. 2020. “Mitochondrial TCA Cycle Metabolites Control Physiology and Disease.” Nature Communications 11 (January): 102. https://doi.org/10.1038/s41467-019-13668-3spa
dc.relation.referencesMasagué, Joan, and Anna C. Obenauf. 2016. “Metastatic Colonization.” Nature 529: 298–306spa
dc.relation.referencesMathew, J, P Sankar, and M Varacallo. 2023. “Physiology, Blood Plasma. [Updated 2023 Apr 24].” StatPearls [Internet]. https://www.ncbi.nlm.nih.gov/books/NBK531504/.spa
dc.relation.referencesMatsui, T, M Amano, T Yamamoto, K Chihara, M Nakafuku, M Ito, T Nakano, K Okawa, A Iwamatsu, and K Kaibuchi. 1996. “Rho-Associated Kinase, a Novel Serine/Threonine Kinase, as a Putative Target for Small GTP Binding Protein Rho.” The EMBO Journal 15 (May): 2208–16spa
dc.relation.referencesMATTSON, M, and R WAN. 2005. “Beneficial Effects of Intermittent Fasting and Caloric Restriction on the Cardiovascular and Cerebrovascular Systems.” The Journal of Nutritional Biochemistry 16 (March): 129–37. https://doi.org/10.1016/j.jnutbio.2004.12.007.spa
dc.relation.referencesMauthe, Mario, Idil Orhon, Cecilia Rocchi, Xingdong Zhou, Morten Luhr, Kerst-Jan Hijlkema, Robert P. Coppes, Nikolai Engedal, Muriel Mari, and Fulvio Reggiori. 2018. “Chloroquine Inhibits Autophagic Flux by Decreasing Autophagosome-Lysosome Fusion.” Autophagy 14 (August): 1435–55. https://doi.org/10.1080/15548627.2018.1474314.spa
dc.relation.referencesMeehan, Sean, and Amrinder S Nain. 2014. “Role of Suspended Fiber Structural Stiffness and Curvature on Single–Cell Migration, Nucleus Shape, and Focal–Adhesion–Cluster Length.” Biophysical Journal 107 (December): 2604–11. https://doi.org/10.1016/j.bpj.2014.09.045.spa
dc.relation.referencesMeng, Qingcai, Jin Xu, Chen Liang, Jiang Liu, Jie Hua, Yiyin Zhang, Quanxing Ni, Si Shi, and Xianjun Yu. 2018. “GPx1 Is Involved in the Induction of Protective Autophagy in Pancreatic Cancer Cells in Response to Glucose Deprivation.” Cell Death & Disease 9 (December): 1187. https://doi.org/10.1038/s41419-018-1244-zspa
dc.relation.referencesMeng, Wenxiang, and Masatoshi Takeichi. 2009. “Adherens Junction: Molecular Architecture and Regulation.” Cold Spring Harbor Perspectives in Biology 1 (December): a002899. https://doi.org/10.1101/cshperspect.a002899.spa
dc.relation.referencesMeulen, Marjolein C. H. van der, and Rik Huiskes. 2002. “Why Mechanobiology?: A Survey Article.” Journal of Biomechanics 35 (4): 401–14. https://doi.org/https://doi.org/10.1016/S0021-9290(01)00184-1spa
dc.relation.referencesModarai, Shirin R., Lynn M. Opdenaker, Vignesh Viswanathan, Jeremy Z. Fields, and Bruce M. Boman. 2016. “Somatostatin Signaling via SSTR1 Contributes to the Quiescence of Colon Cancer Stem Cells.” BMC Cancer 16 (December): 941. https://doi.org/10.1186/s12885- 016-2969-7spa
dc.relation.referencesMohamed, Hoda E., Mervat E. Asker, Nahla S. Kotb, and Akram M. El Habab. 2020. “Human Platelet Lysate Efficiency, Stability, and Optimal Heparin Concentration Required in Culture of Mammalian Cells.” BLOOD RESEARCH 55 (March): 35–43. https://doi.org/10.5045/br.2020.55.1.35spa
dc.relation.referencesMollah, M Z I, H M Zahid, Z Mahal, Mohammad Rashed, and Iqbal Faruque. 2021. “The Usages and Potential Uses of Alginate for Healthcare Applications.” Frontiers in Molecular Biosciences 8: 1–12. https://doi.org/10.3389/fmolb.2021.719972.spa
dc.relation.referencesMoor, Lise De, Sélina Fernandez, Chris Vercruysse, Liesbeth Tytgat, Mahtab Asadian, Nathalie De Geyter, Sandra Van Vlierberghe, Peter Dubruel, and Heidi Declercq. 2020. “Hybrid Bioprinting of Chondrogenically Induced Human Mesenchymal Stem Cell Spheroids.” Frontiers in Bioengineering and Biotechnology 8. https://doi.org/10.3389/fbioe.2020.00484.spa
dc.relation.referencesMuilwijk, Danya, Eyleen de Poel, Peter van Mourik, Sylvia W F Suen, Annelotte M Vonk, Jesse E Brunsveld, Evelien Kruisselbrink, et al. 2022. “Forskolin-Induced Organoid Swelling Is Associated with Long-Term Cystic Fibrosis Disease Progression.” The European Respiratory Journal 60 (August). https://doi.org/10.1183/13993003.00508-2021.spa
dc.relation.referencesMukherjee, Apratim, Bahareh Behkam, and Amrinder S. Nain. 2019. “Cancer Cells Sense Fibers by Coiling on Them in a Curvature–Dependent Manner.” iScience 19: 905–15. https://doi.org/10.1016/j.isci.2019.08.023.spa
dc.relation.referencesMuller, H A, and E Wieschaus. 1996. “Armadillo, Bazooka, and Stardust Are Critical for Early Stages in Formation of the Zonula Adherens and Maintenance of the Polarized Blastoderm Epithelium in Drosophila.” The Journal of Cell Biology 134 (July): 149–63. https://doi.org/10.1083/jcb.134.1.149spa
dc.relation.referencesMurphy, Ciara M., Matthew G. Haugh, and Fergal J. O’Brien. 2010. “The Effect of Mean Pore Size on Cell Attachment, Proliferation and Migration in Collagen–Glycosaminoglycan Scaffolds for Bone Tissue Engineering.” Biomaterials 31: 461–66. https://doi.org/10.1016/j.biomaterials.2009.09.063.spa
dc.relation.referencesMurray, P, G Frampton, and P N Nelson. 1999. “Cell Adhesion Molecules. Sticky Moments in the Clinic.” BMJ (Clinical Research Ed.) 319 (August): 332–34. https://doi.org/10.1136/bmj.319.7206.332.spa
dc.relation.referencesMurrell, Michael, Patrick W. Oakes, Martin Lenz, and Margaret L. Gardel. 2015. “Forcing Cells into Shape: The Mechanics of Actomyosin Contractility.” Nature Reviews Molecular Cell Biology 16 (August): 486–98. https://doi.org/10.1038/nrm4012.spa
dc.relation.referencesNagao, Ayako, Minoru Kobayashi, Sho Koyasu, Christalle C. T. Chow, and Hiroshi Harada. 2019. “HIF-1-Dependent Reprogramming of Glucose Metabolic Pathway of Cancer Cells and Its Therapeutic Significance.” International Journal of Molecular Sciences 20 (January): 238. https://doi.org/10.3390/ijms20020238.spa
dc.relation.referencesNagelkerke, Anika, Johan Bussink, Alan E. Rowan, and Paul N. Span. 2015. “The mechanical microenvironment in cancer: How physics affects tumours.” Seminars in Cancer Biology 35: 62–70. https://doi.org/10.1016/j.semcancer.2015.09.001spa
dc.relation.referencesNairon, Kylie G., Thomas J. DePalma, Joshua M. Zent, Jennifer L. Leight, and Aleksander Skardal. 2022. “Tumor Cell-Conditioned Media Drives Collagen Remodeling via Fibroblast and Pericyte Activation in an in Vitro Premetastatic Niche Model.” iScience 25 (July): 104645. https://doi.org/10.1016/j.isci.2022.104645spa
dc.relation.referencesNakagawa, Osamu, Kazuko Fujisawa, Toshimasa Ishizaki, Yuji Saito, Kazuwa Nakao, and Shuh Narumiya. 1996. “ROCK-i and ROCK-II, Two Isoforms of Rho-Associated Coiled-Coil Forming Protein Serine/Threonine Kinase in Mice.” FEBS Letters 392 (August): 189–93. https://doi.org/10.1016/0014-5793(96)00811-3.spa
dc.relation.referencesNakamura, Tetsuya, and Toshiro Sato. 2018. “Advancing Intestinal Organoid Technology Toward Regenerative Medicine.” Cellular and Molecular Gastroenterology and Hepatology 5 (1): 51–60. https://doi.org/10.1016/j.jcmgh.2017.10.00spa
dc.relation.referencesNakaseko, Yukinobu, and Mitsuhiro Yanagida. 2001. “Cytoskeleton in the Cell Cycle.” Nature 412 (July): 291–92. https://doi.org/10.1038/35085684spa
dc.relation.referencesNam Tay, Puei, Patrick Tan, Yuhong Lan, Carol Ho-Wing Leung, Mirtha Laban, Tze Chin Tan, Hongmin Ni, et al. 2010. “Palladin, an actin-associated protein, is required for adherens junction formation and intercellular adhesion in HCT116 colorectal cancer cells.” International Journal of Oncology 37: 909–26. https://doi.org/10.3892/ijo_00000742.spa
dc.relation.referencesNelson, Richard L, Herand Abcarian, Thomas M. Nelson, Atsunobu Misumi, Hirofumi Kako, Sherif Rizk, and Howard Sky-Peck. 1996. “The Effect of Dietary Selenium Deficiency on Acute Colorectal Mucosal Nucleotoxicity Induced by Several Carcinogens in the Rodent.” The American Journal of Surgery 172 (July): 85–88. https://doi.org/10.1016/S0002- 9610(96)00067-0.spa
dc.relation.referencesNg, Mei Rosa, and Joan S. Brugge. 2009. “A Stiff Blow from the Stroma: Collagen Crosslinking Drives Tumor Progression.” Cancer Cell 16 (6): 455–57. https://doi.org/10.1016/j.ccr.2009.11.013.spa
dc.relation.referencesNguyen, Ryan Y., Hugh Xiao, Xiangyu Gong, Alfredo Arroyo, Aidan T. Cabral, Tom T. Fischer, Kaitlin M. Flores, et al. 2022. “Cytoskeletal Dynamics Regulates Stromal Invasion Behavior of Distinct Liver Cancer Subtypes.” Communications Biology 5 (March): 202. https://doi.org/10.1038/s42003-022-03121-5.spa
dc.relation.referencesNoh, Myung-Giun, Se-Jeong Oh, Eun-Jung Ahn, Yeong-Jin Kim, Tae-Young Jung, Shin Jung, Kyung-Keun Kim, Jae-Hyuk Lee, Kyung-Hwa Lee, and Kyung-Sub Moon. 2017. “Prognostic Significance of e-Cadherin and n-Cadherin Expression in Gliomas.” BMC Cancer 17 (August): 583. https://doi.org/10.1186/s12885-017-3591-zspa
dc.relation.referencesNyberg, Kendra D., Samuel L. Bruce, Angelyn V. Nguyen, Clara K. Chan, Navjot K. Gill, TaeHyung Kim, Erica K. Sloan, and Amy C. Rowat. 2018a. “Predicting cancer cell invasion by single-cell physical phenotyping.” Integrative Biology 10 (4): 218–31. https://doi.org/10.1039/C7IB00222Jspa
dc.relation.references———. 2018b. “Predicting Cancer Cell Invasion by Single-Cell Physical Phenotyping.” Integrative Biology 10 (April): 218–31. https://doi.org/10.1039/C7IB00222J.spa
dc.relation.referencesNyström, Hanna. 2013. “Stromal collagens in colorectal cancer and in colorectal liver metastases: Tumour biological implications and a source for novel tumour markers.” PhD thesis, Umea Universityspa
dc.relation.referencesO’Shea, Christopher, S. Nashitha Kabir, Andrew P. Holmes, Ming Lei, Larissa Fabritz, Kashif Rajpoot, and Davor Pavlovic. 2020. “Cardiac Optical Mapping - State-of-the-Art and Future Challenges.” The International Journal of Biochemistry & Cell Biology 126 (September): 105804. https://doi.org/10.1016/j.biocel.2020.105804spa
dc.relation.referencesOberauer, Klaus. 2022. “The Importance of Random Slopes in Mixed Models for Bayesian Hypothesis Testing.” Psychological Science 33: 648–65. https://doi.org/10.1177/09567976211046884spa
dc.relation.referencesOhshima, Kenji, and Eiichi Morii. 2021. “Metabolic Reprogramming of Cancer Cells During Tumor Progression and Metastasis.” Metabolites 11 (January): 28. https://doi.org/10.3390/metabo11010028spa
dc.relation.referencesOliveira, Rui Caetano, Henrique Alexandrino, Maria Augusta Cipriano, and José Guilherme Tralhão. 2019. “Liver Metastases and Histological Growth Patterns: Biological Behavior and Potential Clinical Implications-Another Path to Individualized Medicine?” Journal of Oncology 2019: 6280347. https://doi.org/10.1155/2019/6280347spa
dc.relation.referencesOlsen, Charlotta J, José Moreira, Eugene M Lukanidin, and Noona S Ambartsumian. 2010. “Human Mammary Fibroblasts Stimulate Invasion of Breast Cancer Cells in a Three– Dimensional Culture and Increase Stroma Development in Mouse Xenografts.” BMC Cancer 10: 444. https://doi.org/10.1186/1471--2407--10--444.spa
dc.relation.referencesOrganization, World Health, ed. n.d. “Cancer Keyfacts.” http://www.who.int/en/newsroom/fact-sheets/detail/cancer.spa
dc.relation.referencesOtranto, Marcela, Vincent Sarrazy, Boris Hinz, and Giulio Gabbiani. 2012. “The Role of the Myofibroblast in Tumor Stroma Remodeling.” Cell Adhesion & Migration, no. 6, 3: 203–19spa
dc.relation.referencesPacheco, Juliana E.Lopes, Carlos Alberto Bavastri, and Jucélio Tomás Pereira. 2015. “Viscoelastic relaxation modulus characterization using Prony series.” Latin American Journal of Solids and Structures 12 (2): 420–45. https://doi.org/10.1590/1679-78251412spa
dc.relation.referencesPajic–Lijakovic, Ivana, Milan Milivojevic, and Andrew G. Clark. 2022. “Collective Cell Migration on Collagen–i Networks: The Impact of Matrix Viscoelasticity.” Frontiers in Cell and Developmental Biology 10: 1–8. https://doi.org/10.3389/fcell.2022.901026spa
dc.relation.referencesPalm, Wilhelm. 2021. “Metabolic Plasticity Allows Cancer Cells to Thrive Under Nutrient Starvation.” Proceedings of the National Academy of Sciences 118 (April). https://doi.org/10.1073/pnas.2102057118.spa
dc.relation.referencesPalorini, Roberta, Giuseppina Votta, Yuri Pirola, Humberto De Vitto, Sara De Palma, Cristina Airoldi, Michele Vasso, et al. 2016. “Protein Kinase a Activation Promotes Cancer Cell Resistance to Glucose Starvation and Anoikis.” PLOS Genetics 12 (March): e1005931. https://doi.org/10.1371/journal.pgen.1005931spa
dc.relation.referencesPamplona, Regina, Sandra González-Lana, Pilar Romero, Ignacio Ochoa, Rafael MartínRapún, and Carlos Sánchez-Somolinos. 2023. “Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for in Vitro Cell Culture Systems.” ACS Applied Polymer Materials 5 (2): 1487–98. https://doi.org/10.1021/acsapm.2c01980spa
dc.relation.referencesPark, Jong–Il, Jisu Lee, Ju–Lee Kwon, Hong–Bum Park, Su–Yel Lee, Ji–Yeon Kim, Jaekye Sung, Jin Man Kim, Kyu Sang Song, and Kyung–Hee Kim. 2016. “Scaffold-Free Coculture Spheroids of Human Colonic Adenocarcinoma Cells and Normal Colonic Fibroblasts Promote Tumorigenicity in Nude Mice.” Translational Oncology 9 (February): 79–88. https://doi.org/10.1016/j.tranon.2015.12.001.spa
dc.relation.referencesPaszek, Matthew J., Nastaran Zahir, Kandice R. Johnson, Johnathon N. Lakins, Gabriela I. Rozenberg, Amit Gefen, Cynthia A. Reinhart-King, et al. 2005. “Tensional homeostasis and the malignant phenotype.” Cancer Cell 8 (3): 241–54. https://doi.org/10.1016/j.ccr.2005.08.010.spa
dc.relation.referencesPaulmurugan, Ramasamy. 2012. Introduction to Cancer Biology. https://doi.org/10.1142/9789814293686_0001spa
dc.relation.referencesPaz–Artigas, Laura, Kaoutar Ziani, Clara Alcaine, Claudia Báez–Díaz, Virginia Blanco– Blázquez, Jose Luis Pedraz, Ignacio Ochoa, and Jesús Ciriza. 2021. “Benefits of Cryopreservation as Long–Term Storage Method of Encapsulated Cardiosphere–Derived Cells for Cardiac Therapy: A Biomechanical Analysis.” International Journal of Pharmaceutics 607. https://doi.org/10.1016/j.ijpharm.2021.121014spa
dc.relation.referencesPeeters, Paul JHL, Marloes T Bazelier, Hubert GM Leufkens, Frank de Vries, and Marie L De Bruin. 2015. “The Risk of Colorectal Cancer in Patients with Type 2 Diabetes: Associations with Treatment Stage and Obesity.” Diabetes Care 38 (3): 495–502spa
dc.relation.referencesPérez-González, Carlos, Gerardo Ceada, Francesco Greco, Marija Matejcic, Manuel GómezGonzález, Natalia Castro, Anghara Menendez, et al. 2021. “Mechanical Compartmentalization of the Intestinal Organoid Enables Crypt Folding and Collective Cell Migration.” Nature Cell Biology 23 (July): 745–57. https://doi.org/10.1038/s41556-021- 00699-6spa
dc.relation.referencesPham S, Puckett Y. 2023. “Physiology, Skeletal Muscle Contraction.” In [Updated 2023 May 1]. Treasure Island (FL): StatPearls Publishingspa
dc.relation.referencesPiechocka, Izabela K., Anne S. G. Van Oosten, Roel G. M. Breuls, and Gijsje H. Koenderink. 2011. “Rheology of Heterotypic Collagen Networks.” Biomacromolecules 12: 2797–2805. https://doi.org/10.1021/bm200553xspa
dc.relation.referencesPietras, Kristian, and Arne Östman. 2010. “Hallmarks of cancer: Interactions with the tumor stroma.” Experimental Cell Research 316 (8): 1324–31. https://doi.org/10.1016/j.yexcr.2010.02.045spa
dc.relation.referencesPinheiro, José, and Douglas Bates. 2000. Mixed-Effects Models in s and s-PLUS. Springer New York, NY. https://doi.org/10.1007/b98882spa
dc.relation.referencesPinheiro, José, Douglas Bates, and R Core Team. 2022. “Nlme: Linear and Nonlinear Mixed Effects Models.” https://cran.r-project.org/package=nlme.spa
dc.relation.referencesPodsypanina, Katrina, Yi-Chieh Nancy Du, Martin Jechlinger, Levi J. Beverly, Dolores Hambardzumyan, and Harold Varmus. 2008. “Seeding and Propagation of Untransformed Mouse Mammary Cells in the Lung.” Science 321: 1841–44. https://doi.org/10.1126/science.1161621.spa
dc.relation.referencesPollard, Thomas D., and John A. Cooper. 2009. “Actin, a Central Player in Cell Shape and Movement.” Science 326 (November): 1208–12. https://doi.org/10.1126/science.1175862spa
dc.relation.referencesPolte, Thomas R., Gabriel S. Eichler, Ning Wang, and Donald E. Ingber. 2004. “Extracellular Matrix Controls Myosin Light Chain Phosphorylation and Cell Contractility Through Modulation of Cell Shape and Cytoskeletal Prestress.” American Journal of Physiology-Cell Physiology 286 (March): C518–28. https://doi.org/10.1152/ajpcell.00280.2003.spa
dc.relation.referencesPoste, George, and Isaiah J. Fidler. 1983. “The Pathogenesis of cancer metastasis.” Nature 283: 139–46. https://doi.org/10.1038/283139a0.spa
dc.relation.referencesPowell, Steven M, Nathan Zilz, Yasmin Beazer-Barclay, Tracy M Bryan, Stanley R Hamilton, Stephen N Thiboudeau, Bert Volgestein, and Kenneth W. Kinzler. 1992. “APC mutations occur early during colorectal tumorigenesis.” Nature 359: 235–37spa
dc.relation.referencesPretzsch, E., F. Bosch, J. Neumann, P. Ganschow, A. Bazhin, M. Guba, J. Werner, and M. Angele. 2019. “Mechanisms of Metastasis in Colorectal Cancer and Metastatic Organotropism: Hematogenous Versus Peritoneal Spread.” Journal of Oncology 2019 (September): 1–13. https://doi.org/10.1155/2019/7407190spa
dc.relation.referencesProvenzano, Paolo P, Kevin W Eliceiri, Jay M Campbell, David R Inman, John G White, and Patricia J Keely. 2006. “Collagen reorganization at the tumor-stromal interface facilitates local invasion.” BMC Medicine 4 (38): 1–15. https://doi.org/10.1186/1741-7015-4-38.spa
dc.relation.referencesProvenzano, Paolo P, David R Inman, Kevin W Eliceiri, Justin G Knittel, Long Yan, Curtis T Rueden, John G White, and Patricia J Keely. 2008. “Collagen Density Promotes Mammary Tumor Initiation and Progression.” BMC Medicine 6: 1–15. https://doi.org/10.1186/1741-- 7015--6--11.spa
dc.relation.referencesQiu, Bo, and M. Celeste Simon. 2015. “Oncogenes Strike a Balance Between Cellular Growth and Homeostasis.” Seminars in Cell & Developmental Biology 43 (July): 3–10. https://doi.org/10.1016/j.semcdb.2015.08.005spa
dc.relation.referencesRaffaghello, Lizzia, Fernando Safdie, Giovanna Bianchi, Tanya Dorff, Luigi Fontana, and Valter D Longo. 2010. “Fasting and Differential Chemotherapy Protection in Patients.” Cell Cycle (Georgetown, Tex.) 9 (November): 4474–76. https://doi.org/10.4161/cc.9.22.13954spa
dc.relation.referencesRatheesh, Aparna, and Alpha S. Yap. 2012. “A Bigger Picture: Classical Cadherins and the Dynamic Actin Cytoskeleton.” Nature Reviews Molecular Cell Biology 13 (October): 673–79. https://doi.org/10.1038/nrm3431spa
dc.relation.referencesRaub, Christopher B., Vinod Suresh, Tatiana Krasieva, Julia Lyubovitsky, Justin D. Mih, Andrew J. Putnam, Bruce J. Tromberg, and Steven C. George. 2007. “Noninvasive Assessment of Collagen Gel Microstructure and Mechanics Using Multiphoton Microscopy.” Biophysical Journal 92: 2212–22. https://doi.org/10.1529/biophysj.106.097998.spa
dc.relation.referencesRaub, Christopher B., Jay Unruh, Vinod Suresh, Tatiana Krasieva, Tore Lindmo, Enrico Gratton, Bruce J. Tromberg, and Steven C. George. 2008. “Image Correlation Spectroscopy of Multiphoton Images Correlates with Collagen Mechanical Properties.” Biophysical Journal 94: 2361–73. https://doi.org/10.1529/biophysj.107.120006.spa
dc.relation.referencesRead, Adam, and Martin Schroder. 2021. “The Unfolded Protein Response: An Overview.” Biology 10 (April): 384. https://doi.org/10.3390/biology10050384spa
dc.relation.referencesReina-Campos, Miguel, Jorge Moscat, and Maria Diaz-Meco. 2017. “Metabolism Shapes the Tumor Microenvironment.” Current Opinion in Cell Biology 48 (October): 47–53. https://doi.org/10.1016/j.ceb.2017.05.006spa
dc.relation.referencesRen, Xiantian, Jie Mi, Huimin Jia, Hong Gao, Yuzuo Bai, and Weilin Wang. 2015. “Reduced Wnt3a Expression Correlates with Poor Development of the Hindgut in Rats with Anorectal Malformations.” Experimental and Molecular Pathology 99 (1): 81–85. https://doi.org/https://doi.org/10.1016/j.yexmp.2015.05.008spa
dc.relation.referencesResearch on Cancer-World Health Organization, International Agency for. n.d. “Estimated number of new cases in 2020, worldwide, both sexes, all ages.” https://gco.iarc.fr/today/online-analysis-pie? v=2020&mode=cancer&mode_population=continents&population=900&populations=900 &key=total&sex=0&cancer=39&type=1&statistic=5&prevalence=0&population_group=0&a ges_group%5B%5D=0&ages_group%5B %5D=17&nb_items=7&group_cancer=1&include_nmsc=1&include_nmsc_other=1&half_pie =0&donut=0.spa
dc.relation.referencesRichardson, Douglas S., Webster Guan, Katsuhiko Matsumoto, Chenchen Pan, Kwanghun Chung, Ali Erturk, Hiroki R. Ueda, and Jeff W. Lichtman. 2021. “Tissue Clearing.” Nature Reviews Methods Primers 1 (December): 84. https://doi.org/10.1038/s43586-021-00080- 9spa
dc.relation.referencesRichbart, Stephen D, Justin C Merritt, Emily G Moles, Kathleen C Brown, Adeoluwa A Adeluola, Paul T Finch, Joshua A Hess, et al. 2022. “Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells.” Bio–Protocol 12: e4320. https://doi.org/10.21769/BioProtoc.4320.spa
dc.relation.referencesRiching, Kristin M., Benjamin L. Cox, Max R. Salick, Carolyn Pehlke, Andrew S. Riching, Susan M. Ponik, Benjamin R. Bass, et al. 2015. “3D collagen alignment limits protrusions to enhance breast cancer cell persistence.” Biophysical Journal 107 (11): 2546–58. https://doi.org/10.1016/j.bpj.2014.10.035.spa
dc.relation.referencesRiedl, Angelika, Michaela Schlederer, Karoline Pudelko, Mira Stadler, Stefanie Walter, Daniela Unterleuthner, Christine Unger, et al. 2016. “Comparison of Cancer Cells Cultured in 2D Vs 3D Reveals Differences in AKT/mTOR/S6-Kinase Signaling and Drug Response.” Journal of Cell Science, January. https://doi.org/10.1242/jcs.188102spa
dc.relation.referencesRivero, Rebeca E., Virginia Capella, A. Cecilia Liaudat, Pablo Bosch, Cesar A. Barbero, Nancy Rodríguez, and Claudia R. Rivarola. 2020. “Mechanical and Physicochemical Behavior of a 3D Hydrogel Scaffold During Cell Growth and Proliferation.” RSC Advances 10: 5827–37. https://doi.org/10.1039/C9RA08162Cspa
dc.relation.referencesRo, Jooyoung, Junyoung Kim, and Yoon-Kyoung Cho. 2022. “Recent Advances in SpheroidBased Microfluidic Models to Mimic the Tumour Microenvironment.” The Analyst 147: 2023–34. https://doi.org/10.1039/D2AN00172A.spa
dc.relation.referencesRobinson, D. N., Y.-S. Kee, T. Luo, and A. Surcel. 2012. “7.5 Understanding How Dividing Cells Change Shape.” Comprehensive Biophysics, 48–72. https://doi.org/10.1016/B978-0- 12-374920-8.00705-0spa
dc.relation.referencesRobledo, Fermín, Lila González-Hodar, Pablo Tapia, Ana-María Figueroa, Fernando Ezquer, and Víctor Cortés. 2023. “Spheroids Derived from the Stromal Vascular Fraction of Adipose Tissue Self-Organize in Complex Adipose Organoids and Secrete Leptin.” Stem Cell Research & Therapy 14 (April): 70. https://doi.org/10.1186/s13287-023-03262-2spa
dc.relation.referencesRodriguez, Luis G, Xiaoyang Wu, and Jun–Lin Guan. 2005. “Wound–Healing Assay.” Methods in Molecular Biology (Clifton, N.J.) 294: 23–29. https://doi.org/10.1385/1--59259--860-- 9:023.spa
dc.relation.referencesRogers, Stephen L, and Vladimir I Gelfand. 2000. “Membrane Trafficking, Organelle Transport, and the Cytoskeleton.” Current Opinion in Cell Biology 12 (February): 57–62. https://doi.org/10.1016/S0955-0674(99)00057-5spa
dc.relation.referencesRoman, Bart I., Sigrid Verhasselt, and Christian V. Stevens. 2018. “Medicinal Chemistry and Use of Myosin II Inhibitor (s)-Blebbistatin and Its Derivatives.” Journal of Medicinal Chemistry 61 (November): 9410–28. https://doi.org/10.1021/acs.jmedchem.8b00503spa
dc.relation.referencesSakamoto, Takeshi, John Limouze, Christian A. Combs, Aaron F. Straight, and James R. Sellers. 2005. “Blebbistatin, a Myosin II Inhibitor, Is Photoinactivated by Blue Light.” Biochemistry 44 (January): 584–88. https://doi.org/10.1021/bi0483357spa
dc.relation.referencesSargenti, Azzurra, Francesco Musmeci, Francesco Bacchi, Cecilia Delprete, Domenico Andrea Cristaldi, Federica Cannas, Simone Bonetti, et al. 2020. “Physical Characterization of Colorectal Cancer Spheroids and Evaluation of NK Cell Infiltration Through a Flow-Based Analysis.” Frontiers in Immunology 11 (December). https://doi.org/10.3389/fimmu.2020.564887spa
dc.relation.referencesSato, Kazunori, Katsuya Tsuchihara, Satoshi Fujii, Masanori Sugiyama, Tomoyuki Goya, Yutaka Atomi, Takashi Ueno, Atsushi Ochiai, and Hiroyasu Esumi. 2007. “Autophagy Is Activated in Colorectal Cancer Cells and Contributes to the Tolerance to Nutrient Deprivation.” Cancer Research 67 (October): 9677–84. https://doi.org/10.1158/0008- 5472.CAN-07-1462spa
dc.relation.referencesSchenk, Susann, Gary J Schoenhals, Gustavo de Souza, and Matthias Mann. 2008. “A High Confidence, Manually Validated Human Blood Plasma Protein Reference Set.” BMC Medical Genomics 1 (December): 41. https://doi.org/10.1186/1755-8794-1-41spa
dc.relation.referencesSchroll, Monica M., Gabriel J. LaBonia, Katelyn R. Ludwig, and Amanda B. Hummon. 2017. “Glucose Restriction Combined with Autophagy Inhibition and Chemotherapy in HCT 116 Spheroids Decreases Cell Clonogenicity and Viability Regulated by Tumor Suppressor Genes.” Journal of Proteome Research 16 (August): 3009–18. https://doi.org/10.1021/acs.jproteome.7b00293spa
dc.relation.referencesSchwager, Samantha C., Paul V. Taufalele, and Cynthia A. Reinhart-King. 2019. “Cell-Cell Mechanical Communication in Cancer.” Cellular and Molecular Bioengineering 12 (February): 1–14. https://doi.org/10.1007/s12195-018-00564-xspa
dc.relation.referencesSeetharaman, Shailaja, and Sandrine Etienne-Manneville. 2020. “Cytoskeletal Crosstalk in Cell Migration.” Trends in Cell Biology 30 (September): 720–35. https://doi.org/10.1016/j.tcb.2020.06.004spa
dc.relation.referencesSeo, Bo Ri, Xingyu Chen, Lu Ling, Young Hye Song, Adrian A. Shimpi, Siyoung Choi, Jacqueline Gonzalez, et al. 2020. “Collagen Microarchitecture Mechanically Controls Myofibroblast Differentiation.” Proceedings of the National Academy of Sciences of the United States of America 117. https://doi.org/10.1073/pnas.1919394117spa
dc.relation.references“Serum for Cell and Tissue Culture: Frequently Asked Questions (FAQs).” n.d. https://www.sigmaaldrich.com/technical-documents/articles/biology/cell-culture/fbsfaq.htmlspa
dc.relation.referencesSeyfoori, Amir, Ehsan Samiei, Neda Jalili, Brent Godau, Mehdi Rahmanian, Leila Farahmand, Keivan Majidzadeh-A, and Mohsen Akbari. 2018. “Self-Filling Microwell Arrays (SFMAs) for Tumor Spheroid Formation.” Lab on a Chip 18: 3516–28. https://doi.org/10.1039/C8LC00708Jspa
dc.relation.referencesSharma, Shivani, Chintda Santiskulvong, Jianyu Rao, James K. Gimzewski, and Oliver Dorigo. 2014. “The Role of Rho GTPase in Cell Stiffness and Cisplatin Resistance in Ovarian Cancer Cells.” Integr. Biol. 6: 611–17. https://doi.org/10.1039/C3IB40246K.spa
dc.relation.referencesSims, John R., Seth Karp, and Donald E. Ingber. 1992a. “Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape.” Journal of Cell Science 103 (4): 1215–22. https://doi.org/10.1242/jcs.103.4.1215spa
dc.relation.references———. 1992b. “Altering the Cellular Mechanical Force Balance Results in Integrated Changes in Cell, Cytoskeletal and Nuclear Shape.” Journal of Cell Science 103 (December): 1215–22. https://doi.org/10.1242/jcs.103.4.1215.spa
dc.relation.referencesSong, Zhou, Bo Wei, Canrong Lu, Peiyu Li, and Lin Chen. 2017. “Glutaminase Sustains Cell Survival via the Regulation of Glycolysis and Glutaminolysis in Colorectal Cancer.” Oncology Letters 14 (September): 3117–23. https://doi.org/10.3892/ol.2017.6538spa
dc.relation.referencesSouza, Glauco R., Jennifer R. Molina, Robert M. Raphael, Michael G. Ozawa, Daniel J. Stark, Carly S. Levin, Lawrence F. Bronk, et al. 2010. “Three-Dimensional Tissue Culture Based on Magnetic Cell Levitation.” Nature Nanotechnology 5 (April): 291–96. https://doi.org/10.1038/nnano.2010.23spa
dc.relation.referencesSpada, Martina, Cristina Piras, Giulia Diana, Vera Piera Leoni, Daniela Virginia Frau, Gabriele Serreli, Gabriella Simbula, et al. 2023. “Glutamine Starvation Affects Cell Cycle, Oxidative Homeostasis and Metabolism in Colorectal Cancer Cells.” Antioxidants 12 (March): 683. https://doi.org/10.3390/antiox12030683spa
dc.relation.referencesSpill, Fabian, Daniel S. Reynolds, Roger D. Kamm, and Muhammad H. Zaman. 2016. “Impact of the physical microenvironment on tumor progression and metastasis.” Current Opinion in Biotechnology 40: 41–48. https://doi.org/10.1016/j.copbio.2016.02.007.spa
dc.relation.referencesSrichaikul, Korbua, Robert A. Hegele, and David J. A. Jenkins. 2022. “Great Chinese Famine and the Effects on Cardiometabolic Health for Future Generations.” Hypertension 79 (3): 532–35. https://doi.org/10.1161/HYPERTENSIONAHA.121.18546.spa
dc.relation.referencesStankovic, Tijana, Teodora Randelovic, Miodrag Dragoj, Sonja Stojkovic Buric, Luis Fernández, Ignacio Ochoa, Victor M. Pérez-García, and Milica Pesic. 2021. “In Vitro Biomimetic Models for Glioblastoma-a Promising Tool for Drug Response Studies.” Drug Resistance Updates 55 (March): 100753. https://doi.org/10.1016/j.drup.2021.100753spa
dc.relation.referencesSteeg, Patricia S. 2006. “Tumor Metastasis: Mechanistic Insights and Clinical Challenges.” Nature Medicine 12: 895–904. https://doi.org/10.1038/nm1469spa
dc.relation.referencesSung, Hyuna, Jacques Ferlay, Rebecca L Siegel, Mathieu Laversanne, Isabelle Soerjomataram, Ahmedin Jemal, and Freddie Bray. 2021. “Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.” CA: A Cancer Journal for Clinicians 71 (3): 209–49spa
dc.relation.referencesSuzuki, Atsushi, Gen-ichi Kusakai, Atsuhiro Kishimoto, Yuji Minegichi, Tsutomu Ogura, and Hiroyasu Esumi. 2003. “Induction of Cell-Cell Detachment During Glucose Starvation Through f-Actin Conversion by SNARK, the Fourth Member of the AMP-Activated Protein Kinase Catalytic Subunit Family.” Biochemical and Biophysical Research Communications 311 (November): 156–61. https://doi.org/10.1016/j.bbrc.2003.09.184spa
dc.relation.referencesSweeney, H. Lee, and David W. Hammers. 2018. “Muscle Contraction.” Cold Spring Harbor Perspectives in Biology 10 (February): a023200. https://doi.org/10.1101/cshperspect.a023200.spa
dc.relation.referencesTaddei, ML, E Giannoni, T Fiaschi, and P Chiarugi. 2012. “Anoikis: An Emerging Hallmark in Health and Diseases.” The Journal of Pathology 226 (January): 380–93. https://doi.org/10.1002/path.3000.spa
dc.relation.referencesTakatsuna, Masafumi, Satoko Morohashi, Tadashi Yoshizawa, Hideaki Hirai, Toshihiro Haga, Rie Ota, Kensuke Saito, et al. 2016. “Myofibroblast distribution is associated with invasive growth types of colorectal cancer.” Oncology Reports 36 (6): 3154–60. https://doi.org/10.3892/or.2016.5202spa
dc.relation.referencesTan, Juan, Hao-Lian Wang, Jie Yang, Qian-Qian Liu, Chun-Min Li, Yun-Qian Wang, Lin-Na Fu, Qin-Yan Gao, Ying-Xuan Chen, and Jing-Yuan Fang. 2020. “JMJD2B-Induced Amino Acid Alterations Enhance the Survival of Colorectal Cancer Cells Under Glucose-Deprivation via Autophagy.” Theranostics 10: 5763–77. https://doi.org/10.7150/thno.38087spa
dc.relation.referencesTateishi, K., W. Ando, C. Higuchi, D. A. Hart, J. Hashimoto, K. Nakata, H. Yoshikawa, and N. Nakamura. 2008. “Comparison of Human Serum with Fetal Bovine Serum for Expansion and Differentiation of Human Synovial MSC: Potential Feasibility for Clinical Applications.” Cell Transplantation 17 (5): 549–57. https://doi.org/10.3727/096368908785096024spa
dc.relation.referencesTauriello, Daniele V. F., Alexandre Calon, Enza Lonardo, and Eduard Batlle. 2017. “Determinants of metastatic competency in colorectal cancer.” Molecular Oncology 11 (1): 97–119. https://doi.org/10.1002/1878-0261.12018spa
dc.relation.referencesTeam, R Core. 2013. “R: A Language and Environment for Statistical Computing.” R Foundation for Statistical Computing. http://www.r-project.org/spa
dc.relation.references“The Role of Actin and Myosin.” n.d. http://encyclopedia.lubopitko-bg.com/The_Role_of_Actin_and_Myosin.html.spa
dc.relation.referencesTian, B., B’Ann T. Gabelt, P. L. Kaufman, and B. Geiger. 2010. “The Cytoskeletal Network of the Trabecular Meshwork.” In Encyclopedia of the Eye, 549–55. Elsevier. https://doi.org/10.1016/B978-0-12-374203-2.00098-1.spa
dc.relation.referencesTidwell, Tia R., Gro V. Rosland, Karl Johan Tronstad, Kjetil Søreide, and Hanne R. Hagland. 2022. “Metabolic Flux Analysis of 3D Spheroids Reveals Significant Differences in Glucose Metabolism from Matched 2D Cultures of Colorectal Cancer and Pancreatic Ductal Adenocarcinoma Cell Lines.” Cancer & Metabolism 10 (December): 9. https://doi.org/10.1186/s40170-022-00285-wspa
dc.relation.referencesTiffon, Céline. 2018. “The Impact of Nutrition and Environmental Epigenetics on Human Health and Disease.” International Journal of Molecular Sciences 19 (November). https://doi.org/10.3390/ijms19113425.spa
dc.relation.referencesTozluoğlu, Melda, Alexander L Tournier, Robert P Jenkins, Steven Hooper, Paul A Bates, and Erik Sahai. 2013. “Matrix Geometry Determines Optimal Cancer Cell Migration Strategy and Modulates Response to Interventions.” Nature Cell Biology 15 (7): 751–62spa
dc.relation.referencesTrayhurn, Paul. 2019. “Oxygen-a Critical, but Overlooked, Nutrient.” Frontiers in Nutrition 6 (February). https://doi.org/10.3389/fnut.2019.00010spa
dc.relation.referencesTrepat, Xavier, Zaozao Chen, and Ken Jacobson. 2012. “Cell Migration.” Comprehensive Physiology 2 (October): 2369–92. https://doi.org/10.1002/cphy.c110012spa
dc.relation.referencesTsai, Pei-Yun, Min-Sik Lee, Unmesh Jadhav, Insia Naqvi, Shariq Madha, Ashley Adler, Meeta Mistry, et al. 2021. “Adaptation of Pancreatic Cancer Cells to Nutrient Deprivation Is Reversible and Requires Glutamine Synthetase Stabilization by mTORC1.” Proceedings of the National Academy of Sciences 118 (March). https://doi.org/10.1073/pnas.2003014118spa
dc.relation.referencesUshiki, Tatsuo. 2002. “Collagen Fibers, Reticular Fibers and Elastic Fibers. A Comprehensive Understanding from a Morphological Viewpoint.” Archives of Histology and Cytology 65 (2): 109–26. https://doi.org/10.1679/aohc.65.109spa
dc.relation.referencesUshiki, Tatsuo, and Chizuka Ide. 1986. “Three–Dimensional Architecture of the Endoneurium with Special Reference to the Collagen Fibril Arrangement in Relation to Nerve Fibers.” Archivum Histologicum Japonicum 49: 553–63. https://doi.org/10.1679/aohc.49.553.spa
dc.relation.referencesVadivelu, Raja, Harshad Kamble, Muhammad Shiddiky, and Nam-Trung Nguyen. 2017. “Microfluidic Technology for the Generation of Cell Spheroids and Their Applications.” Micromachines 8 (March): 94. https://doi.org/10.3390/mi8040094spa
dc.relation.referencesVaghela, Ravikumar, Andreas Arkudas, Raymund E Horch, and Maximilian Hessenauer. 2021. “Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.” Frontiers in Bioengineering and Biotechnology 9. https://doi.org/10.3389/fbioe.2021.627462spa
dc.relation.referencesVanharanta, Sakari, and Joan Massagué. 2014. “Origins of Metastatic Traits.” Cancer Cell 24 (4): 410–21. https://doi.org/10.1016/j.ccr.2013.09.007.Originsspa
dc.relation.referencesViallard, V., C. Denis, V. Trocheris, and J. C. Murat. 1986. “Effect of Glutamine Deprivation and Glutamate or Ammonium Chloride Addition on Growth Rate, Metabolism and Differentiation of Human Colon Cancer Cell-Line HT29.” International Journal of Biochemistry 18 (January): 263–69. https://doi.org/10.1016/0020-711X(86)90116-3spa
dc.relation.referencesVignes, Helene, Christina Vagena-Pantoula, and Julien Vermot. 2022. “Mechanical Control of Tissue Shape: Cell-Extrinsic and -Intrinsic Mechanisms Join Forces to Regulate Morphogenesis.” Seminars in Cell & Developmental Biology 130 (October): 45–55. https://doi.org/10.1016/j.semcdb.2022.03.017spa
dc.relation.referencesVinci, Maria, Carol Box, and Suzanne A Eccles. 2015. “Three–Dimensional (3D) Tumor Spheroid Invasion Assay.” JoVE, e52686. https://doi.org/doi:10.3791/52686spa
dc.relation.referencesVirumbrales-Muñoz, María, Laura Paz-Artigas, Jesús Ciriza, Clara Alcaine, Albert EsponaNoguera, Manuel Doblaré, Laura Sáenz Del Burgo, et al. 2021. “Force Spectroscopy Imaging and Constriction Assays Reveal the Effects of Graphene Oxide on the Mechanical Properties of Alginate Microcapsules.” ACS Biomaterials Science and Engineering 7: 242–53. https://doi.org/10.1021/acsbiomaterials.0c01382spa
dc.relation.referencesWaldherr, Linda, Maria Seitanidou, Marie Jakesová, Verena Handl, Sophie Honeder, Marta Nowakowska, Tamara Tomin, et al. 2021. “Targeted Chemotherapy of Glioblastoma Spheroids with an Iontronic Pump.” Advanced Materials Technologies 6 (May): 2001302. https://doi.org/10.1002/admt.202001302spa
dc.relation.referencesWalker, Alyssa, Anju Singh, Ellen Tully, Juhyung Woo, Anne Le, Tu Nguyen, Shyam Biswal, Dipali Sharma, and Edward Gabrielson. 2018. “Nrf2 Signaling and Autophagy Are Complementary in Protecting Breast Cancer Cells During Glucose Deprivation.” Free Radical Biology & Medicine 120 (May): 407–13. https://doi.org/10.1016/j.freeradbiomed.2018.04.009spa
dc.relation.referencesWang, Fuhan, Ni Sun, Hanqin Zeng, Yuan Gao, Naisheng Zhang, and Wenlong Zhang. 2022. “Selenium Deficiency Leads to Inflammation, Autophagy, Endoplasmic Reticulum Stress, Apoptosis and Contraction Abnormalities via Affecting Intestinal Flora in Intestinal Smooth Muscle of Mice.” Frontiers in Immunology 13 (July). https://doi.org/10.3389/fimmu.2022.947655spa
dc.relation.referencesWatanabe, Kiichi, Morio Ueno, Daisuke Kamiya, Ayaka Nishiyama, Michiru Matsumura, Takafumi Wataya, Jun B Takahashi, et al. 2007. “A ROCK Inhibitor Permits Survival of Dissociated Human Embryonic Stem Cells.” Nature Biotechnology 25 (June): 681–86. https://doi.org/10.1038/nbt1310spa
dc.relation.referencesWeigert, Roberto, Monika Sramkova, Laura Parente, Panomwat Amornphimoltham, and Andrius Masedunskas. 2010. “Intravital Microscopy: A Novel Tool to Study Cell Biology in Living Animals.” Histochemistry and Cell Biology 133 (May): 481–91. https://doi.org/10.1007/s00418--010--0692--zspa
dc.relation.referencesWeinberg, Robert A. 2014. The Biology of Cancer. Garland Sciencespa
dc.relation.referencesWen, Qi, and Paul A Janmey. 2011. “Polymer Physics of the Cytoskeleton.” Current Opinion in Solid State & Materials Science 15 (October): 177–82. https://doi.org/10.1016/j.cossms.2011.05.002spa
dc.relation.references“What Are Cell-Cell Adhesions?” n.d. https://www.mechanobio.info/what-ismechanosignaling/what-are-cell-cell-adhesions/spa
dc.relation.references“What Are Cell-Matrix Adhesions?” n.d. https://www.mechanobio.info/what-ismechanosignaling/what-is-the-extracellular-matrix-and-the-basal-lamina/spa
dc.relation.references“What Is the Cytoskeleton?” n.d. https://www.mechanobio.info/cytoskeleton-dynamics/what-is-the-cytoskeleton/#whatis-the-cytoskeletonspa
dc.relation.referencesWhitley, Elise, and Jonathan Ball. 2002a. “Statistics Review 3: Hypothesis Testing and p Values.” Critical Care 6: 222. https://doi.org/10.1186/cc149spa
dc.relation.references———. 2002b. “Statistics Review 5: Comparison of Means.” Critical Care (London, England) 6 (October): 424–28. https://doi.org/10.1186/cc1548spa
dc.relation.references———. 2002c. “Statistics Review 6: Nonparametric Methods.” Critical Care (London, England) 6 (December): 509–13. https://doi.org/10.1186/cc1820spa
dc.relation.referencesWinters, Brian S., Scott R. Shepard, and Ramsey A. Foty. 2005. “Biophysical Measurement of Brain Tumor Cohesion.” International Journal of Cancer 114 (April): 371–79. https://doi.org/10.1002/ijc.20722spa
dc.relation.referencesWirtz, Denis, Konstantinos Konstantopoulos, and Peter C Searson. 2011. “The Physics of Cancer: The Role of Physical Interactions and Mechanical Forces in Metastasis.” Nature Reviews. Cancer 11 (June): 512–22. https://doi.org/10.1038/nrc3080spa
dc.relation.referencesWu, Hao, Zonghui Ding, Danqing Hu, Feifei Sun, Chunyan Dai, Jiansheng Xie, and Xun Hu. 2012. “Central Role of Lactic Acidosis in Cancer Cell Resistance to Glucose DeprivationInduced Cell Death.” The Journal of Pathology 227 (June): 189–99. https://doi.org/10.1002/path.3978spa
dc.relation.referencesWu, Ting, and Yun Dai. 2017. “Tumor microenvironment and therapeutic response.” Cancer Letters 387: 61–68. https://doi.org/10.1016/j.canlet.2016.01.043spa
dc.relation.referencesXie, Jing, Min Bao, Stéphanie M. C. Bruekers, and Wilhelm T. S. Huck. 2017. “Collagen Gels with Different Fibrillar Microarchitectures Elicit Different Cellular Responses.” ACS Applied Materials and Interfaces 9: 19630–37. https://doi.org/10.1021/acsami.7b03883spa
dc.relation.referencesXu, Li, and Anthony Bretscher. 2014. “Rapid Glucose Depletion Immobilizes Active Myosin v on Stabilized Actin Cables.” Current Biology 24 (October): 2471–79. https://doi.org/10.1016/j.cub.2014.09.017spa
dc.relation.referencesYang, Ya Li, and Laura J. Kaufman. 2009. “Rheology and Confocal Reflectance Microscopy as Probes of Mechanical Properties and Structure During Collagen and Collagen/Hyaluronan Self–Assembly.” Biophysical Journal 96: 1566–85. https://doi.org/10.1016/j.bpj.2008.10.063spa
dc.relation.referencesYang, Ya Li, Lindsay M. Leone, and Laura J. Kaufman. 2009b. “Elastic Moduli of Collagen Gels Can Be Predicted from Two–Dimensional Confocal Microscopy.” Biophysical Journal 97: 2051–60. https://doi.org/10.1016/j.bpj.2009.07.035spa
dc.relation.references———. 2009a. “Elastic Moduli of Collagen Gels Can Be Predicted from Two–Dimensional Confocal Microscopy.” Biophysical Journal 97: 2051–60. https://doi.org/10.1016/j.bpj.2009.07.035spa
dc.relation.referencesYang, Ya Li, Stéphanie Motte, and Laura J. Kaufman. 2010. “Pore Size Variable Type i Collagen Gels and Their Interaction with Glioma Cells.” Biomaterials 31: 5678–88. https://doi.org/10.1016/j.biomaterials.2010.03.03spa
dc.relation.referencesYonemura, Shigenobu, Masahiko Itoh, Akira Nagafuchi, and Shoichiro Tsukita. 1995. “Cellto-Cell Adherens Junction Formation and Actin Filament Organization: Similarities and Differences Between Non-Polarized Fibroblasts and Polarized Epithelial Cells.” Journal of Cell Science 108 (January): 127–42. https://doi.org/10.1242/jcs.108.1.127spa
dc.relation.referencesYu, Hongmei, Janna Kay Mouw, and Valerie M. Weaver. 2011. “Forcing form and function: Biomechanical regulation of tumor evolution.” Trends in Cell Biology 21 (1): 47–56. https://doi.org/10.1016/j.tcb.2010.08.015spa
dc.relation.referencesYu, Weibo, Shivani Sharma, Elizabeth Rao, Amy C. Rowat, James K. Gimzewski, Dong Han, and Jianyu Rao. 2022. “Cancer Cell Mechanobiology: A New Frontier for Cancer Research.” Journal of the National Cancer Center 2 (1): 10–17. https://doi.org/https://doi.org/10.1016/j.jncc.2021.11.007spa
dc.relation.referencesYue, Kan, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Ali Tamayol, Nasim Annabi, and Ali Khademhosseini. 2015. “Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels.” Biomaterials 73: 254–71. https://doi.org/10.1016/j.biomaterials.2015.08.045spa
dc.relation.referencesZhao, Ru-Zhou, Shuai Jiang, Lin Zhang, and Zhi-Bin Yu. 2019. “Mitochondrial Electron Transport Chain, ROS Generation and Uncoupling (Review).” International Journal of Molecular Medicine, May. https://doi.org/10.3892/ijmm.2019.4188spa
dc.relation.referencesZhao, Zixuan, Xinyi Chen, Anna M. Dowbaj, Aleksandra Sljukic, Kaitlin Bratlie, Luda Lin, Eliza Li Shan Fong, et al. 2022. “Organoids.” Nature Reviews Methods Primers 2 (December): 94. https://doi.org/10.1038/s43586-022-00174-yspa
dc.relation.referencesZhong, Xinyang, Xuefeng He, Yaxian Wang, Zijuan Hu, Huixia Huang, Senlin Zhao, Ping Wei, and Dawei Li. 2022. “Warburg Effect in Colorectal Cancer: The Emerging Roles in Tumor Microenvironment and Therapeutic Implications.” Journal of Hematology & Oncology 15 (November): 160. https://doi.org/10.1186/s13045-022-01358-5spa
dc.relation.referencesZhou, Yunfei, Federico Tozzi, Jinyu Chen, Fan Fan, Ling Xia, Jinrong Wang, Guang Gao, et al. 2012. “Intracellular ATP Levels Are a Pivotal Determinant of Chemoresistance in Colon Cancer Cells.” Cancer Research 72 (January): 304–14. https://doi.org/10.1158/0008- 5472.CAN-11-1674spa
dc.relation.referencesZibaei, Zohre, Esmaeil Babaei, Arezoo Rezaie Nezhad Zamani, Reza Rahbarghazi, and Hewa Jalal Azeez. 2021. “Curcumin-Enriched Gemini Surfactant Nanoparticles Exhibited Tumoricidal Effects on Human 3D Spheroid HT-29 Cells in Vitro.” Cancer Nanotechnology 12 (December): 3. https://doi.org/10.1186/s12645-020-00074-4spa
dc.relation.referencesZihni, Ceniz, Clare Mills, Karl Matter, and Maria S Balda. 2016. “Tight Junctions: From Simple Barriers to Multifunctional Molecular Gates.” Nature Reviews Molecular Cell Biology 17: 564–80. https://doi.org/10.1038/nrm.2016.80spa
dc.relation.referencesZlobec, Inti, Martin D. Berger, and Alessandro Lugli. 2020. “Tumour Budding and Its Clinical Implications in Gastrointestinal Cancers.” British Journal of Cancer 123 (September): 700– 708. https://doi.org/10.1038/s41416-020-0954-zspa
dc.relation.referencesZocher, M. A., S. E. Groves, and D. H. Allen. 1997. “A three-dimensional finite element formulation for thermoviscoelastic orthotropic media.” International Journal for Numerical Methods in Engineering. https://doi.org/10.1002/(SICI)1097- 0207(19970630)40:12<2267::AID-NME156>3.0.CO;2-Pspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.ddc610 - Medicina y salud::616 - Enfermedadesspa
dc.subject.ddc620 - Ingeniería y operaciones afinesspa
dc.subject.decsNeoplasias Colorrectalesspa
dc.subject.decsColorectal Neoplasmseng
dc.subject.decsTécnicas In Vitrospa
dc.subject.decsIn Vitro Techniqueseng
dc.subject.decsFenómenos Fisiológicos Celularesspa
dc.subject.decsCell Physiological Phenomenaeng
dc.subject.proposalCancereng
dc.subject.proposalColonspa
dc.subject.proposalSpheroidseng
dc.subject.proposalMigrationeng
dc.subject.proposalInvasioneng
dc.subject.proposalCollageneng
dc.subject.proposalHydrogelseng
dc.subject.proposalMechanobiologyeng
dc.subject.proposalCytoskeletoneng
dc.subject.proposalContractioneng
dc.subject.proposalCáncerspa
dc.subject.proposalColonspa
dc.subject.proposalEsferoidesspa
dc.subject.proposalMigraciónspa
dc.subject.proposalInvasiónspa
dc.subject.proposalColágenospa
dc.subject.proposalHidrogelesspa
dc.subject.proposalMecanobiologíaspa
dc.subject.proposalCitoesqueletospa
dc.subject.proposalContracciónspa
dc.titleIn vitro modeling of mechanical and biochemical aspects involved in the cellular migratory processes related with colorectal cancer metastasiseng
dc.title.translatedModelado in vitro de los aspectos mecánicos y bioquímicos involucrados en procesos migratorios celulares relacionados con la metástasis de cáncer colorrectalspa
dc.typeTrabajo de grado - Doctoradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_db06spa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/doctoralThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TDspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

Archivos

Bloque original

Mostrando 1 - 1 de 1
Miniatura
Nombre:
1070948613.2023.pdf
Tamaño:
93.28 MB
Formato:
Adobe Portable Document Format
Descripción:
Tesis de Doctorado en Ingeniería - Ingeniería Mecánica y Mecatrónica
Descargar

Bloque de licencias

Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
5.74 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Doctorado en Ingeniería - Ingeniería Mecánica y Mecatrónica