Deslignificación de desechos de Rosa sp utilizando el hongo basidiomiceto selectivo ceriporiopsis subvermispora
| dc.contributor.advisor | Velásquez Lozano, Mario Enrique | spa |
| dc.contributor.advisor | Nieto Ramírez, Ivonne Jeannette | spa |
| dc.contributor.author | Chávez Arias, Nelson Enrique | spa |
| dc.contributor.researchgroup | Grupo de Investigación en Procesos Químicos y Bioquímicos | spa |
| dc.date.accessioned | 2020-08-22T03:56:17Z | spa |
| dc.date.available | 2020-08-22T03:56:17Z | spa |
| dc.date.issued | 2020-02-24 | spa |
| dc.description.abstract | La Rosa spp es el principal tipo de flor cultivada en Colombia con aproximadamente 33% de participación en la producción florícola del país. La alta cantidad de desechos vegetales producidos en este cultivo hacen de este material un sustrato interesante para ser explotado en procesos biotecnológicos. En el presente trabajo se utilizó el hongo basidiomiceto Ceriporiopsis subvermispora con el fin de evaluar su potencial para el tratamiento de estos residuos. Se estudió su capacidad para colonizar un sustrato semisólido a base de residuos de Rosa sp, en términos de crecimiento micelial, producción de lacasas y porcentaje de descomposición de lignina para 15 días de tratamiento. Con un modelo logístico se determinó el efecto del pH y la adición de sales, encontrando que el hongo es capaz de crecer eficientemente sobre el sustrato sin la necesidad del uso de nutrientes y reguladores buffer. El efecto de la temperatura y la dilución sobre el método de extracción de lacasas se determinó, observando que la máxima recuperación fue posible con una dilución de 1/60 y 30 °C para este medio de cultivo. Se determinó la actividad lacasa durante este periodo encontrando la máxima producción al día 15 (1,69 U/g ± 020). Finalmente se cuantificó el decaimiento de lignina durante la colonización del hongo, llegando a descomponer 3,54% del total de lignina en comparación al blanco. | spa |
| dc.description.abstract | The Rose spp is the main type of flower planted in Colombia, with approximately 33% stake in the country's production floriculture. The high amount of plant waste produced in this industry, make this material an interesting substrate to be exploited in biotechnological processes. In this paper the fungus basidiomycete Ceriporiopsis subvermispora was used to evaluate its potential for the bio-treatment of this waste. Its ability to colonize a substrate of solid waste-Rose sp was studied, using as response variable the mycelial growth, production of laccase and lignin decay for 15 days. Using a logistic model, the effect of pH and the addition of salts was determined. It was found that the microorganism is able to grow efficiently on the substrate without the need for use of nutrients and buffer. The effect dela temperature and dilution in the extraction process was determined laccases. It was found that maximum recovery was possible with a 1/60 dilution and 30 ° C. It was determined laccase activity during this period, finding the maximum production at day 15 (1.69 U / g ± 020). Finally, the lignin decomposition was quantitated during colonization of the fungus, reaching the maximum rate of delignification at 15 days with 3.54% of degraded lignin. | spa |
| dc.description.additional | Línea de Investigación: Bioprocesos Productos Naturales Vegetales | spa |
| dc.description.degreelevel | Maestría | spa |
| dc.description.sponsorship | Fundación CEIBA | spa |
| dc.format.extent | 130 | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/78165 | |
| dc.language.iso | spa | spa |
| dc.publisher.branch | Universidad Nacional de Colombia - Sede Bogotá | spa |
| dc.publisher.program | Bogotá - Ingeniería - Maestría en Ingeniería - Ingeniería Química | spa |
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| dc.rights | Derechos reservados - Universidad Nacional de Colombia | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.license | Atribución-NoComercial-SinDerivadas 4.0 Internacional | spa |
| dc.rights.spa | Acceso abierto | spa |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | spa |
| dc.subject.ddc | 500 - Ciencias naturales y matemáticas | spa |
| dc.subject.ddc | 660 - Ingeniería química | spa |
| dc.subject.ddc | 570 - Biología | spa |
| dc.subject.ddc | 600 - Tecnología (Ciencias aplicadas) | spa |
| dc.subject.proposal | crecimiento micelial | spa |
| dc.subject.proposal | laccase | eng |
| dc.subject.proposal | lignin | eng |
| dc.subject.proposal | lacasa | spa |
| dc.subject.proposal | waste-Rose sp | eng |
| dc.subject.proposal | lignina | spa |
| dc.subject.proposal | desechos de Rosa sp | spa |
| dc.subject.proposal | ceriporiopsis subvermispora | eng |
| dc.subject.proposal | ceriporiopsis subvermispora | spa |
| dc.subject.proposal | mycelial growth | eng |
| dc.title | Deslignificación de desechos de Rosa sp utilizando el hongo basidiomiceto selectivo ceriporiopsis subvermispora | spa |
| dc.type | Trabajo de grado - Maestría | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
| dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | spa |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |

