Development of a NiOxHy/chitosan-MWCNTs modified screen-printed electrode for dual detection of glucose and insulin

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dc.contributor.advisorEstupiñan Durán, Hugo Armando
dc.contributor.authorSantacruz Zambrano, Solvey Isleny
dc.contributor.orcidESTUPIÑAN DURAN, HUGO ARMANDO [0000000296073364]
dc.contributor.researchgroupGrupo de Investigación en Biosuperficies
dc.date.accessioned2026-02-04T18:20:30Z
dc.date.available2026-02-04T18:20:30Z
dc.date.issued2026-02-03
dc.descriptionIlustraciones
dc.description.abstractThe development of advanced electrochemical sensors depends on the rational design and optimization of functional materials with high electrocatalytic activity, stability, and sensitivity. This thesis reports the synthesis, integration, and electrochemical evaluation of a non enzymatic sensing platform based on nickel oxyhydroxide-decorated multi-walled carbon nanotubes (NiOxHy@MWCNTs) dispersed in a chitosan matrix and deposited onto gold screen printed electrodes (SPEs) for the separate detection of glucose and insulin. The NiOxHy @MWCNTs composite was synthesized via hydrothermal treatment using nickel nitrate hexahydrate as the precursor and characterized by Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) to confirm its structural, morphological, and elemental properties. Electrochemical characterization using cyclic voltammetry (CV), linear sweep voltammetry (LSV), chronoamperometry, and electrochemical impedance spectroscopy (EIS) revealed that SPEs modified with the 2 mM hydrothermal powder exhibited the most favorable interfacial properties: the lar gest electroactive surface area (Randles–Ševčík analysis), highest double layer capacitance (Cdl), and lowest charge-transfer resistance (Rct). These attributes translated into superior analytical performance for both glucose and insulin detection. Compared with unmodified SPEs, the modified electrodes displayed enhanced current responses and well defined redox features for both analytes, confirming the role of the NiOxHy@MWCNTs–chitosan composite in improving electron transfer and catalytic activity. Overall, these findings demonstrate the potential of the optimized 2 mM NiOxHy@MWCNTs chitosan/SPE platform as a cost-effective and versatile sensing material for targeted analyte detection, contributing to the advancement of next-generation electrochemical sensor technologies with applications in miniaturized and point-of-care diagnostic devices.eng
dc.description.abstractEl desarrollo de sensores electroquímicos avanzados depende del diseño racional y la optimización de materiales funcionales con alta actividad electrocatalítica, estabilidad y sensibilidad. Esta tesis presenta la síntesis, integración y evaluación electroquímica de una plataforma de detección no enzimática basada en nanotubos de carbono de paredes múltiples decorados con oxihidróxido de níquel (NiOxHy@MWCNTs), dispersos en una matriz de quitosano y depositados sobre electrodos serigrafiados de oro (SPEs) para la detección independiente de glucosa e insulina. El compuesto NiOxHy@MWCNTs se sintetizó mediante tratamiento hidrotermal utilizando como precursor el nitrato de níquel hexahidratado, y fue caracterizado por espectroscopía Raman, difracción de rayos X (XRD), microscopía electrónica de barrido (SEM) y espectroscopía de dispersión de energía de rayos X (EDS) para confirmar sus propiedades estructurales, morfológicas y elementales. La caracterización electroquímica, mediante CV, LSV, y EIS, reveló que los SPEs modificados con el polvo hidrotermal 2 mM presentaron las propiedades interfaciales más favorables: la mayor área electroactiva (análisis de Randles–Ševčík), la capacitancia de doble capa (Cdl) más alta y la menor resistencia a la transferencia de carga (Rct). Estos atributos se tradujeron en un rendimiento analítico superior para la detección de glucosa e insulina. En comparación con los SPEs sin modificar, los electrodos modificados mostraron respuestas de corriente mejoradas y picos redox bien definidos para ambos analitos, confirmando el papel del compuesto NiOxHy@MWCNTs–quitosano en la mejora de la transferencia electrónica y la actividad catalítica. En conjunto, estos resultados demuestran el potencial de la plataforma optimizada 2 mM NiOxHy@MWCNTs–quitosano/SPE como material sensor versátil y de bajo costo para la detección dirigida de analitos, contribuyendo al avance de las tecnologías de sensores electroquímicos de nueva generación con aplicaciones en dispositivos miniaturizados y de diagnóstico en el punto de atención. (Texto tomado de la fuente)spa
dc.description.curricularareaMateriales Y Nanotecnología.Sede Medellín
dc.description.degreelevelMaestría
dc.description.degreenameMagíster en Ingeniería - Materiales y Procesos
dc.description.researchareaElectroquímica Aplicada a Sensores
dc.format.extent1 recurso en líne (108 páginas)
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/89394
dc.language.isoeng
dc.publisherUniversidad Nacional de Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellín
dc.publisher.facultyFacultad de Minas
dc.publisher.placeMedellín, Colombia
dc.publisher.programMedellín - Minas - Maestría en Ingeniería - Materiales y Procesos
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dc.relation.referencesT. Donnor and S. Sarkar, “Insulin- Pharmacology, Therapeutic Regimens and Principles of Intensive Insulin Therapy,” Endotext, Feb. 2023, Accessed: Aug. 08, 2025. [Online]. Available: https://www.ncbi.nlm.nih.gov/books/NBK278938/
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseAtribución-SinDerivadas 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc610 - Medicina y salud
dc.subject.ddc620 - Ingeniería y operaciones afines
dc.subject.lembElectoquímica
dc.subject.lembBiosensores
dc.subject.lembMarcadores bioquimicos
dc.subject.lembMateriales biomédicos
dc.subject.proposalNon-enzymatic electrochemical sensoreng
dc.subject.proposalSensor electroquímico no enzimáticospa
dc.subject.proposalNickel oxyhydroxide (NiOxHy)eng
dc.subject.proposalOxihidróxido de níquel (NiOxHy)spa
dc.subject.proposalMulti walled carbon nanotubes (MWCNTs)eng
dc.subject.proposalNanotubos de carbono de paredes múltiples (MWCNTs)spa
dc.subject.proposalGlucose and insulin detectioneng
dc.subject.proposalDetección de glucosa e insulinaspa
dc.titleDevelopment of a NiOxHy/chitosan-MWCNTs modified screen-printed electrode for dual detection of glucose and insulineng
dc.title.translatedDesarrollo de un electrodo impreso modificado con NiOxHy/quitosano-MWCNTs para la detección dual de glucosa e insulinaspa
dc.typeTrabajo de grado - Maestría
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dcterms.audience.professionaldevelopmentInvestigadores
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2

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