Caracterización bioquímica, funcional y biológica del veneno de Crotalus durissus cumanensis colombiana

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dc.contributor.advisorVega Castro, Nohora Angélica
dc.contributor.advisorUmaña Pérez, Yadi Adriana
dc.contributor.authorRodríguez Vargas, Ariadna Lorena
dc.contributor.cvlacRODRIGUEZ VARGAS, ARIADNA LORENA [0001378971]spa
dc.contributor.googlescholarRodriguez Vargas, Ariadna [m3BS1-EAAAAJ&hl=es]spa
dc.contributor.orcidRodríguez-Vargas Ariadna [000000016636987X]spa
dc.contributor.researchgateRodriguez Vargas Ariadna Lorena [Ariadna-Rodriguez-Vargas]spa
dc.contributor.researchgroupGrupo de Investigación en Proteinas Gripspa
dc.date.accessioned2024-03-19T16:05:34Z
dc.date.available2024-03-19T16:05:34Z
dc.date.issued2023
dc.descriptionilustraciones, fotografias, diagramasspa
dc.description.abstractEn el veneno de serpientes cascabel predomina la actividad neurotóxica de las fosfolipasas tipo A2 (PLA2) y las miotoxinas de bajo peso molecular, además de los efectos hemocitotóxicos relacionados con proteasas. Se evaluaron las diferencias bioquímicas y biológicas en los venenos de Crotalus durissus cumanensis de tres ecorregiones de Colombia: Magdalena Medio (MM), Caribe (CA) y Orinoquía (OR). Se realizaron SDSPAGE y HPLC, letalidad, desfibrinación, procoagulación y actividades enzimáticas. Se realizó el reconocimiento de los antivenenos, mediante determinación de dosis efectiva (ED), Western blotting (WB) y ELISA. En los tres venenos, se encontraron proteasas y PLA2, incluida la subunidad básica de crotoxina. Solo se detectó crotamina en el veneno de CA. Hubo mayor letalidad, actividad coagulante, actividad fosfolipasa A2 y actividad hialuronidasa para el veneno de MM. Se encontraron diferencias en el reconocimiento de los antivenenos de uso comercial, colombiano y mexicano, por WB e inmunoafinidad. Existe variabilidad intraespecífica, considerando las diferencias en la abundancia e intensidad de los componentes, además de la actividad y respuesta a los antivenenos. La lectina tipo C (CTL) del veneno pudo ser aislada parcialmente y aparece unida a una serinoproteasa, al parecer por efecto de su glicosilación. Esta CTL mostró un efecto proliferativo sobre líneas celulares. Se purificó crotamina y se modeló su estructura tridimensional. Se demostró su efecto sobre la línea celular de cáncer de pulmón A549, mostrando un interesante efecto pro-apoptótico. Además, se hizo un docking molecular con una canal de potasio dependiente de voltaje, que también estaría involucrado en su efecto citotóxico. (Texto tomado de la fuente)spa
dc.description.abstractIn rattlesnake venom, the neurotoxic activity of phospholipase A2 (PLA2) and low molecular weight myotoxins predominates, in addition to protease-related hemocytotoxic effects. Biochemical and biological differences in Crotalus durissus cumanensis venoms from three ecoregions of Colombia: Magdalena Medio (MM), Caribe (CA) and Orinoquía (OR) were evaluated. SDS-PAGE and HPLC, lethality, defibrination, procoagulation, and enzymatic activities were performed. Recognition of antivenoms was achieved by determination of effective dose (ED), Western blotting (WB) and ELISA. In all three venoms, proteases and PLA2, including the basic crotoxin subunit, were found. Crotamine was only detected in CA venom. There was higher lethality, coagulant activity, phospholipase A2 activity, and hyaluronidase activity for the MM venom. Differences were found in the recognition of commercially used antivenoms, Colombian and Mexican, by WB and immunoaffinity. There is intraspecific variability, considering the differences in the abundance and intensity of the components, as well as the activity and response to antivenoms. The C-type lectin (CTL) of the venom could be partially isolated and appears bound to a serineprotease, apparently due to its glycosylation. This CTL showed a proliferative effect on cell lines. Crotamine was purified and its three-dimensional structure was modeled. Its effect on the A549 lung cancer cell line was demonstrated, showing an interesting pro-apoptotic effect. In addition, a molecular docking with a voltage-gated potassium channel was made, which would also be involved in its cytotoxic effect.eng
dc.description.degreelevelDoctoradospa
dc.description.degreenameDoctora en Ciencias - Bioquímicaspa
dc.description.researchareaEstudio de lectinas de venenos animalesspa
dc.description.sponsorshipMinciencias | Ministerio de Ciencia Tecnología e Innovaciónspa
dc.description.sponsorshipColfuturospa
dc.format.extentxxvii, 292 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85817
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Doctorado en Ciencias - Bioquímicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.lembSerpientes venenosasspa
dc.subject.lembSnake venomeng
dc.subject.lembVenenosspa
dc.subject.lembPoisonseng
dc.subject.proposalCrotalus durissus cumanensisspa
dc.subject.proposalVenenospa
dc.subject.proposalLectinas tipo Cspa
dc.subject.proposalCrotaminaspa
dc.subject.proposalCitotoxicidadspa
dc.subject.proposalVenomeng
dc.subject.proposalC-type lectinseng
dc.subject.proposalCrotamineeng
dc.subject.proposalCytotoxicityeng
dc.titleCaracterización bioquímica, funcional y biológica del veneno de Crotalus durissus cumanensis colombianaspa
dc.title.translatedBiochemical, functional and biological characterization of the Colombian Crotalus durissus cumanensis venomeng
dc.title.translatedCaractérisation biochimique, fonctionnelle et biologique du venin colombien de Crotalus durissus cumanensisfra
dc.typeTrabajo de grado - Doctoradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_db06spa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/doctoralThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TDspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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