Identificación de receptores para una proteína del merozoíto de Plasmodium vivax en células hospederas de malaria

Molina Franky, Jessica Stephanie

Identificación de receptores para una proteína del merozoíto de Plasmodium vivax en células hospederas de malaria

dc.contributor.advisor	Patarroyo Gutiérrez, Manuel Alfonso	spa
dc.contributor.advisor	Kalkum, Markus	spa
dc.contributor.author	Molina Franky, Jessica Stephanie	spa
dc.contributor.researchgroup	Department of Immunology and Theranostics City of Hope	spa
dc.contributor.researchgroup	Fundación Instituto de Inmunología de Colombia (FIDIC)	spa
dc.contributor.researchgroup	Grupo de Investigación Básica en Biología Molecular e Inmunología (GIBBMI)	spa
dc.date.accessioned	2025-05-22T19:09:04Z
dc.date.available	2025-05-22T19:09:04Z
dc.date.issued	2024-12-16
dc.description	ilustraciones, diagramas, fotografías	spa
dc.description.abstract	La malaria humana es causada principalmente por Plasmodium vivax y Plasmodium falciparum, siendo estas las especies más extendidas y letales, respectivamente. El estudio de P. vivax enfrenta desafíos debido a su invasión exclusiva de reticulocitos, células precursoras de los eritrocitos. La maduración rápida y la escasa disponibilidad de los reticulocitos han dificultado el desarrollo de un cultivo continuo in vitro que permita estudiar la biología y las interacciones receptor-ligando del parásito. En este contexto, se exploró el potencial de las líneas celulares eritroides JK-1 y BEL-A2 como alternativas a los reticulocitos, confirmándose su idoneidad mediante la invasión exitosa del parásito. Además, se empleó cromatografía líquida acoplada a espectrometría de masas (LC-MS/MS) para realizar una comparación cuantitativa de los proteomas de membrana de estas líneas celulares en relación con los reticulocitos y los eritrocitos maduros. Este análisis reveló similitudes significativas entre los proteomas de membrana de JK-1, BEL-A2 y los reticulocitos. Adicionalmente, se identificaron potenciales candidatos a receptores, como SLC7A5, SLC7A1, SLC1A5, CD36, ITGB1, PHB2 y CNNM3, que podrían desempeñar un papel en la vía de invasión específica del parásito. Finalmente, se evaluó la utilidad de ensayos de captura por afinidad asociado a LC-MS/MS, realizando una prueba de concepto sobre la interacción entre PfRH5 y BSG. Esta técnica sentó las bases para identificar los receptores de PvRBP1a157-650, fusionada con TurboID en líneas celulares eritroides. Mediante esta técnica de etiquetado por proximidad, se biotinilaron los receptores en contacto con el ligando, permitiendo la identificación de interacciones específicas entre PvRBP1a157-650 con el receptor de transferrina 1 (CD71), basigina y prohibitina-2, confirmadas por monitoreo de reacciones paralelas y verificadas en su especificidad y constantes de afinidad mediante ELISA. Cabe resaltar que este es el primer reporte que describe a PHB2 como receptor en la interacción de P. vivax con su célula diana. Estos hallazgos enriquecen el conocimiento sobre las interacciones receptor-ligando de P. vivax y refuerzan la relevancia de las células JK-1 y BEL-A2 como modelos celulares alternativos en su estudio. (Texto tomado de la fuente).	spa
dc.description.abstract	Human malaria is primarily caused by Plasmodium vivax and Plasmodium falciparum, which are the most widespread and lethal species, respectively. The study of P. vivax presents challenges due to its exclusive invasion of reticulocytes, the precursor cells of erythrocytes. The rapid maturation and limited availability of reticulocytes have hindered the development of continuous in vitro cultures to study the biology and receptor-ligand interactions of the parasite. In this context, the potential of the erythroid cell lines JK-1 and BEL-A2 was explored as alternatives to reticulocytes, with their suitability confirmed through the successful invasion of the parasite. Furthermore, liquid chromatography coupled with mass spectrometry (LC-MS/MS) was used to quantitatively compare the membrane proteomes of these cell lines in relation to reticulocytes and mature erythrocytes. This analysis revealed significant similarities between the membrane proteomes of JK-1, BEL-A2, and reticulocytes. Furthermore, potential receptor candidates were identified, including SLC7A5, SLC7A1, SLC1A5, CD36, ITGB1, PHB2, and CNNM3, which may play a role in the specific invasion pathway of the parasite. Finally, the utility of affinity capture assays associated with LC-MS/MS was evaluated, conducting a proof of concept on the interaction between PfRH5 and BSG. This technique laid the groundwork for identifying the receptors of PvRBP1a157-650, fused with TurboID, in the erythroid cell lines. Using this proximity labeling technique, receptors in contact with the ligand were biotinylated, allowing for the identification of specific interactions between PvRBP1a157-650 and transferrin receptor protein 1 (CD71), basigin, and prohibitin-2. These interactions were confirmed through monitoring of parallel reactions and verified for their specificity and binding constants using ELISA, representing the first report of PHB2 as a receptor for P. vivax. These findings enhance the understanding of receptor-ligand interactions in P. vivax and underscore the significance of JK-1 and BEL-A2 cells as alternative cellular models for their study.	eng
dc.description.degreelevel	Doctorado	spa
dc.description.degreename	Doctor en Biotecnología	spa
dc.format.extent	199 páginas + 6 anexos	spa
dc.format.mimetype	application/pdf	spa
dc.identifier.instname	Universidad Nacional de Colombia	spa
dc.identifier.reponame	Repositorio Institucional Universidad Nacional de Colombia	spa
dc.identifier.repourl	https://repositorio.unal.edu.co	spa
dc.identifier.uri	https://repositorio.unal.edu.co/handle/unal/88183
dc.language.iso	spa	spa
dc.publisher	Universidad Nacional de Colombia	spa
dc.publisher.branch	Universidad Nacional de Colombia - Sede Bogotá	spa
dc.publisher.faculty	Facultad de Ciencias	spa
dc.publisher.place	Bogotá, Colombia	spa
dc.publisher.program	Bogotá - Ciencias - Doctorado en Biotecnología	spa
dc.relation.indexed	Bireme	spa
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dc.rights.accessrights	info:eu-repo/semantics/openAccess	spa
dc.rights.license	Atribución-NoComercial-SinDerivadas 4.0 Internacional	spa
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	spa
dc.subject.ddc	570 - Biología::572 - Bioquímica	spa
dc.subject.decs	Malaria Vivax	spa
dc.subject.decs	Malaria, Vivax	eng
dc.subject.proposal	Plasmodium vivax	spa
dc.subject.proposal	Líneas celulares	spa
dc.subject.proposal	Eritroides	spa
dc.subject.proposal	Proteoma de membrana	spa
dc.subject.proposal	Interacciones receptor-ligando	spa
dc.subject.proposal	PvRBP1a	spa
dc.subject.proposal	TurboID	spa
dc.subject.proposal	Espectrometría de masas	spa
dc.subject.proposal	Plasmodium vivax	eng
dc.subject.proposal	Erythroid cell lines	eng
dc.subject.proposal	Membrane proteome	eng
dc.subject.proposal	Receptor-ligand interactions	eng
dc.subject.proposal	PvRBP1a	eng
dc.subject.proposal	TurboID	eng
dc.subject.proposal	Mass spectrometry	eng
dc.subject.wikidata	proteoma	spa
dc.subject.wikidata	proteome	eng
dc.subject.wikidata	Proteínas de la célula huésped	spa
dc.subject.wikidata	Host cell protein	eng
dc.title	Identificación de receptores para una proteína del merozoíto de Plasmodium vivax en células hospederas de malaria	spa
dc.title.translated	Identification of receptors for a Plasmodium vivax merozoite protein in malaria host cells	eng
dc.type	Trabajo de grado - Doctorado	spa
dc.type.coar	http://purl.org/coar/resource_type/c_db06	spa
dc.type.coarversion	http://purl.org/coar/version/c_ab4af688f83e57aa	spa
dc.type.content	Text	spa
dc.type.driver	info:eu-repo/semantics/doctoralThesis	spa
dc.type.redcol	http://purl.org/redcol/resource_type/TD	spa
dc.type.version	info:eu-repo/semantics/acceptedVersion	spa
dcterms.audience.professionaldevelopment	Estudiantes	spa
dcterms.audience.professionaldevelopment	Investigadores	spa
dcterms.audience.professionaldevelopment	Público general	spa
oaire.accessrights	http://purl.org/coar/access_right/c_abf2	spa