Asociación genómica de la resistencia a la enfermedad del virus de la raya marrón de la yuca (Manihot esculenta), en accesiones de germoplasma conservadas en América del Sur

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dc.contributor.advisorCarvajal Yepes, Mónica
dc.contributor.advisorLópez Álvarez, Diana Carolina
dc.contributor.authorOspina Colorado, Jessica Alejandra
dc.contributor.cvlacJessica Alejandra Ospinaspa
dc.contributor.googlescholarJessica Alejandra Ospina Coloradospa
dc.contributor.orcidJessica Alejandra Ospina 0009-0009-9662-630Xspa
dc.contributor.researchgateJessica Alejandra Ospinaspa
dc.contributor.researchgroupAgrobiodiversidad y Biotecnologíaspa
dc.contributor.researchgroupBiotecnologia Vegetalspa
dc.contributor.scopusJessica Alejandra Ospinaspa
dc.date.accessioned2024-07-25T20:31:41Z
dc.date.available2024-07-25T20:31:41Z
dc.date.issued2024
dc.descriptionIlustraciones, mapas, tablasspa
dc.description.abstractLa yuca (Manihot esculenta), constituye una fuente primaria de ingresos y calorías dietéticas para millones de personas. Sin embargo, la producción de este cultivo en el África del Este es considerablemente afectada por la enfermedad del virus de la raya marrón de la yuca (CBSD, por sus siglas en inglés, Cassava brown streak disease), causada por dos especies de virus, el virus de la raya marrón de la yuca (CBSV, por sus siglas en inglés, Cassava brown streak virus) y el virus de la raya marrón de la yuca de Uganda (UCBSV, por sus siglas en inglés, Ugandan cassava brown streak virus). El objetivo de esta investigación fue realizar un análisis de asociación genómica, para identificar regiones genómicas asociadas a la resistencia al virus y la enfermedad en un panel de 234 accesiones de germoplasma fenotipadas previamente, por un grupo de investigación independiente. Las muestras fueron genotipadas mediante secuenciación, lo que resultó en un total de 121.405 SNPs. Tras aplicar filtros para la selección de muestras y marcadores de calidad e informativos, evaluamos con la herramienta Gapit v3.0 cuatro modelos estadísticos el Modelo Lineal General (GLM), el Modelo Lineal Mixto (MLM), el Modelo lineal mixto Multilocus (MLMM), y BLINK, comparamos los resultados y realizamos la anotación de los marcadores estadísticamente significativos. Durante el estudio, identificamos 27 nuevos marcadores SNPs distribuidos en varios cromosomas, asociados a la severidad de los síntomas causados por el virus o relacionados con la presencia o ausencia del mismo. De estos marcadores, uno había sido reportado anteriormente; los demás se ubicaron dentro o cerca de genes previamente identificados con funciones relacionadas al reconocimiento de patógenos y la activación de la respuesta inmune. Además, identificamos 30 accesiones de yuca que contenían un alto número de los alelos encontrados, lo que sugiere su potencial resistencia al virus. Los resultados de este estudio representan una valiosa contribución al pool genético para el mejoramiento de la yuca contra esta enfermedad. Así mismo, respaldan y fomentan el uso informado de los materiales conservados en la Alianza Bioversity Int. & CIAT. (Texto tomado de la fuente)spa
dc.description.abstractCassava (Manihot esculenta) serves as a primary source of income and dietary calories for millions of people. However, its production in East Africa is significantly impacted by Cassava Brown Streak Disease (CBSD), caused by two virus species: Cassava Brown Streak Virus (CBSV) and Ugandan Cassava Brown Streak Virus (UCBSV). The aim of this research was to conduct a genome-wide association analysis to identify genomic regions associated with resistance to the virus and disease in a panel of 234 previously phenotyped germplasm accessions by an independent research group. The samples were genotyped through sequencing, resulting in a total of 121,405 SNPs. After applying filters for sample and marker selection, we evaluated four statistical models General Linear Model (GLM), Linear Mixed Model (MLM), Multiple Loci Mixed Model (MLMM), and BLINK using the Gapit v3.0 tool, compared the results, and annotated statistically significant markers. During the study, we identified 27 new SNPs markers distributed across various chromosomes, associated with the severity of symptoms caused by the virus, or related to its presence or absence. Of these markers, one had been reported previously; the rest were located within or near genes previously identified with functions related to pathogen recognition and immune response activation. Additionally, we identified 30 cassava accessions containing a high number of alleles found, suggesting their potential resistance to the virus. The results of this study represent a valuable contribution to the genetic pool for cassava improvement against this disease. They also support and encourage the informed use of materials conserved in the Seeds of the Future collection of the Bioversity International Int. & CIAT Alliance.eng
dc.description.curricularareaCiencias Agropecuarias.Sede Palmiraspa
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Biológicasspa
dc.description.methodsEl objetivo de esta investigación fue realizar un análisis de asociación genómica, para identificar regiones genómicas asociadas a la resistencia al virus y la enfermedad en un panel de 234 accesiones de germoplasma fenotipadas previamente, por un grupo de investigación independiente. Las muestras fueron genotipadas mediante secuenciación, lo que resultó en un total de 121.405 SNPs. Tras aplicar filtros para la selección de muestras y marcadores de calidad e informativos, evaluamos con la herramienta Gapit v3.0 cuatro modelos estadísticos el Modelo Lineal General (GLM), el Modelo Lineal Mixto (MLM), el Modelo lineal mixto Multilocus (MLMM), y BLINK, comparamos los resultados y realizamos la anotación de los marcadores estadísticamente significativos.spa
dc.description.researchareaBiotecnología vegetalspa
dc.format.extentxvi, 78 páginas + anexosspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/86627
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Nivel Nacionalspa
dc.publisher.facultyFacultad de Ciencias Agrariasspa
dc.publisher.placePalmira, Valle del Cauca, Colombiaspa
dc.publisher.programPalmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicasspa
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dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.agrovocResistencia a agentes dañinos
dc.subject.agrovocResistance to injurious factors
dc.subject.agrovocResistencia genética
dc.subject.agrovocGenetic resistance
dc.subject.agrovocControl genético
dc.subject.agrovocGenetic control
dc.subject.agrovocConservación de recursos genéticos
dc.subject.agrovocGenetic resources conservation
dc.subject.agrovocRecurso genético
dc.subject.agrovocRenetic resources
dc.subject.ddc630 - Agricultura y tecnologías relacionadasspa
dc.subject.proposalYucaspa
dc.subject.proposalCassava brown streak disease (CBSD)eng
dc.subject.proposalCassava brown streak virus (CBSV)eng
dc.subject.proposalGenotipificaciónspa
dc.subject.proposalEstudio de asociación del genoma completo (GWAS)spa
dc.subject.proposalGermoplasmaspa
dc.subject.proposalCassava (Manihot esculenta)eng
dc.subject.proposalGenotypingeng
dc.subject.proposalGermplasmeng
dc.titleAsociación genómica de la resistencia a la enfermedad del virus de la raya marrón de la yuca (Manihot esculenta), en accesiones de germoplasma conservadas en América del Surspa
dc.title.translatedGenomic Association of Resistance to Brown Streak Virus Disease in Cassava (Manihot esculenta) in Germplasm Accessions Conserved in South Americaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.fundernameCentro Internacional de Agricultura Tropical CIATspa

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Maestría en Ciencias Biológicas