En 21 día(s), 15 hora(s) y 8 minuto(s): El Repositorio Institucional UNAL informa a la comunidad universitaria que, con motivo del periodo de vacaciones colectivas, el servicio de publicación estará suspendido: Periodo de cierre: Del 20 de diciembre al 18 de enero de 2026. Sobre los depósitos: Durante este tiempo, los usuarios podrán continuar realizando el depósito respectivo de sus trabajos en la plataforma. Reanudación: Una vez reiniciadas las actividades administrativas, los documentos serán revisados y publicados en orden de llegada.

Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide

Vista sencilla de ítem
dc.contributor.advisorCastellanos Parra, Jaime Eduardo
dc.contributor.advisorRomero Sánchez, María Consuelo
dc.contributor.authorCastillo Perdomo, Diana Marcela
dc.contributor.cvlacCastillo Perdomo, Diana Marcela [https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000293580]spa
dc.contributor.googlescholarCastillo, Diana Marcela [https://scholar.google.com/citations?user=_DyKomkAAAAJ&hl=es]spa
dc.contributor.orcidCastillo, Diana Marcela [0000-0002-1846-7448]spa
dc.contributor.researchgateCastillo, Diana Marcela [https://www.researchgate.net/profile/Diana-Castillo-4]spa
dc.contributor.researchgroupUnidad de Investigación Básica Oral - UIBOspa
dc.contributor.scopusCastillo, Diana Marcela [16300829800]spa
dc.contributor.subjectmatterexpertLafaurie Villamil Gloria Inés
dc.date.accessioned2023-02-06T16:52:57Z
dc.date.available2023-02-06T16:52:57Z
dc.date.issued2022-12-02
dc.descriptionilustraciones, graficasspa
dc.description.abstractEstudios clínicos y epidemiológicos han corroborado la asociación bidireccional entre la artritis reumatoide (AR) y la periodontitis. Esta asociación es atribuida a Porphyromonas gingivalis, por la presencia de la enzima peptidil arginina deiminasa (PPAD) y con la actividad de las Arg-gingipaínas en la citrulinación, vinculadas con el quiebre de la tolerancia inmunológica en la AR. Se ha confirmado que los anticuerpos anti-P. gingivalis acompañan el desarrollo de la AR. Sin embargo, pocos estudios indagan la asociación de factores de virulencia específicos de esta bacteria con la AR. En este estudio hemos producido la PPAD de manera recombinante y purificado la gingipaína RgpA nativa a partir de vesículas de membrana externa de P. gingivalis ATCC33277, la actividad enzimática se evaluó con sustratos fluorogénicos. La presencia de las proteínas se confirmó por SDS-PAGE y Western Blot y fueron usadas como antígenos en la detección de los anticuerpos contra estos factores de virulencia mediante ELISA en 143 pacientes con AR y 112 individuos sin AR. Se encontraron diferencias significativas en los títulos de anti-RgpA entre los dos grupos y se determinó asociación con el diagnóstico de AR (OR 4.09; IC 95% 1.2-13.9), mientras que los anti-PPAD no presentan diferencias. La interacción anti-RgpA/anti-PPAD se asoció con diagnósticos de AR (OR 6.63; IC 95% 1.61-27). Además, se purificaron con éxito los dos factores de virulencia de P. gingivalis y se desarrolló el ELISA, permitiendo la detección de los anticuerpos frente a estas proteínas. Los anticuerpos anti-RgpA y la doble positividad de los anticuerpos anti-RgpA/anti-PPAD pueden ser marcadores diagnósticos de la AR. (Texto tomado de la fuente)spa
dc.description.abstractClinical and epidemiological studies have confirmed the bidirectional association between rheumatoid arthritis (RA) and periodontitis. This association is attributed to Porphyromonas gingivalis, due to the presence of peptidyl arginine deiminase (PPAD) together with the action of Arg-gingipains in citrullination, linked to the alteration of immunological tolerance in RA. It has been confirmed that anti-P. gingivalis accompany the development of RA. Nevertheless. Few studies investigate the association of specific virulence factors of this bacterium with RA. In this study we have recombinantly produced PPAD and purified native RgpA gingipain from outer membrane vesicles of P. gingivalis ATCC33277, the enzymatic activity was evaluated with fluorogenic substrates. The presence of the proteins was confirmed by SDS-PAGE and Western Blot and they were used as antigens in the detection of antibodies against these virulence factors by ELISA in 143 patients with RA and 112 individuals without RA. Significant differences were found in anti-RgpA titers between the two groups and an association with RA was determined (OR 4.09; 95% CI 1.2-13.9), while anti-PPAD did not present differences. The anti-RgpA/anti-PPAD interaction was associated with RA diagnoses (OR 6.63; 95% CI 1.61-27). In addition, the two virulence factors of P. gingivalis were successfully purified and the ELISA was developed, allowing the detection of antibodies against these proteins. Anti-RgpA antibodies and the double positivity of anti-RgpA/anti-PPAD antibodies can be diagnostic markers of RA.eng
dc.description.degreelevelDoctoradospa
dc.description.degreenameDoctor en Biotecnologíaspa
dc.description.researchareaMicrobiología Oralspa
dc.description.sponsorshipMinciencias- Ministerio de Ciencia Tecnología e Innovación. Contrato: 642-2017 Código: 130874455642spa
dc.format.extentxxi, 96 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.cospa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83320
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Doctorado en Biotecnologíaspa
dc.relation.referencesAdams, B., Nunes, J. M., Page, M. J., Roberts, T., Carr, J., Nell, T. A., Kell, D. B., & Pretorius, E. (2019). Parkinson's Disease: A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens. Frontiers in aging neuroscience, 11, 210. https://doi.org/10.3389/fnagi.2019.00210spa
dc.relation.referencesAletaha, D., Neogi, T., Silman, A. J., Funovits, J., Felson, D. T., Bingham, C. O., 3rd, Birnbaum, N. S., Burmester, G. R., Bykerk, V. P., Cohen, M. D., Combe, B., Costenbader, K. H., Dougados, M., Emery, P., Ferraccioli, G., Hazes, J. M., Hobbs, K., Huizinga, T. W., Kavanaugh, A., Kay, J., … Hawker, G. (2010). 2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Arthritis and rheumatism, 62(9), 2569–2581. https://doi.org/10.1002/art.27584spa
dc.relation.referencesAmbrosio, N., Marín, M. J., Laguna, E., Herrera, D., Sanz, M., & Figuero, E. (2019). Detection and quantification of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in bacteremia induced by interdental brushing in periodontally healthy and periodontitis patients. Archives of oral biology, 98, 213–219. https://doi.org/10.1016/j.archoralbio.2018.11.025spa
dc.relation.referencesArévalo-Caro, C., Romero-Sánchez, C., & Garavito-Rodríguez, E. (2022). Relation between anti-Porphyromonas gingivalis antibody titers and HLA-DRB1 neutral alleles in individuals with rheumatoid arthritis. Acta odontologica Scandinavica, 80(2), 131–139. https://doi.org/10.1080/00016357.2021.1959053spa
dc.relation.referencesAzzi, L., Rania, S., Vinci, R., Spadari, F., Croveri, F., Scognamiglio, C., Farronato, D., Tettamanti, L., Tagliabue, A., Silvestre-Rangil, J., & Bellintani, C. (2017). Periodontal microbioma and rheumatoid arthritis: The role of Porhyromonas gingivalis. Journal of biological regulators and homeostatic agents, 31(2 Suppl 1), 97–103.spa
dc.relation.referencesBae, S. C., & Lee, Y. H. (2018). Association between anti-Porphyromonas gingivalis antibody, anti-citrullinated protein antibodies, and rheumatoid arthritis: A meta-analysis. Zeitschrift fur Rheumatologie, 77(6), 522–532. https://doi.org/10.1007/s00393-017-0328-yspa
dc.relation.referencesBello-Gualtero, J. M., Lafaurie, G. I., Hoyos, L. X., Castillo, D. M., De-Avila, J., Munevar, J. C., Unriza, S., Londoño, J., Valle-Oñate, R., & Romero-Sánchez, C. (2016). Periodontal Disease in Individuals With a Genetic Risk of Developing Arthritis and Early Rheumatoid Arthritis: A Cross-Sectional Study. Journal of periodontology, 87(4), 346–356. https://doi.org/10.1902/jop.2015.150455spa
dc.relation.referencesBender, P., Bürgin, W. B., Sculean, A., & Eick, S. (2017). Serum antibody levels against Porphyromonas gingivalis in patients with and without rheumatoid arthritis - a systematic review and meta-analysis. Clinical oral investigations, 21(1), 33–42. https://doi.org/10.1007/s00784-016-1938-5spa
dc.relation.referencesBereta, G., Goulas, T., Madej, M., Bielecka, E., Solà, M., Potempa, J., & Xavier Gomis-Rüth, F. (2019). Structure, function, and inhibition of a genomic/clinical variant of Porphyromonas gingivalis peptidylarginine deiminase. Protein science: a publication of the Protein Society, 28(3), 478–486. https://doi.org/10.1002/pro.3571spa
dc.relation.referencesBıyıkoğlu, B., Buduneli, N., Aksu, K., Nalbantsoy, A., Lappin, D. F., Evrenosoğlu, E., & Kinane, D. F. (2013). Periodontal therapy in chronic periodontitis lowers gingival crevicular fluid interleukin-1beta and DAS28 in rheumatoid arthritis patients. Rheumatology international, 33(10), 2607–2616. https://doi.org/10.1007/s00296-013-2781-5spa
dc.relation.referencesBonfim, M., Mattos, F. F., Ferreira e Ferreira, E., Campos, A. C., & Vargas, A. M. (2013). Social determinants of health and periodontal disease in Brazilian adults: a cross-sectional study. BMC oral health, 13, 22. https://doi.org/10.1186/1472-6831-13-22spa
dc.relation.referencesBorgnakke W. S. (2015). Does Treatment of Periodontal Disease Influence Systemic Disease?. Dental clinics of North America, 59(4), 885–917. https://doi.org/10.1016/j.cden.2015.06.007spa
dc.relation.referencesBotero, J. E., Contreras, A., Lafaurie, G., Jaramillo, A., Betancourt, M., & Arce, R. M. (2007). Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. Journal of periodontology, 78(4), 696–704. https://doi.org/10.1902/jop.2007.060129spa
dc.relation.referencesBoutaga, K., van Winkelhoff, A. J., Vandenbroucke-Grauls, C. M., & Savelkoul, P. H. (2003). Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples. Journal of clinical microbiology, 41(11), 4950–4954. https://doi.org/10.1128/JCM.41.11.4950-4954.2003spa
dc.relation.referencesCantley, M. D., Haynes, D. R., Marino, V., & Bartold, P. M. (2011). Pre-existing periodontitis exacerbates experimental arthritis in a mouse model. Journal of clinical periodontology, 38(6), 532–541. https://doi.org/10.1111/j.1600-051X.2011.01714.xspa
dc.relation.referencesCastillo-Cañón, J. C., Trujillo-Cáceres, S. J., Bautista-Molano, W., Valbuena-García, A. M., Fernández-Ávila, D. G., & Acuña-Merchán, L. (2021). Rheumatoid arthritis in Colombia: a clinical profile and prevalence from a national registry. Clinical rheumatology, 40(9), 3565–3573. https://doi.org/10.1007/s10067-021-05710-xspa
dc.relation.referencesCastillo, D. M., Castillo, Y., Delgadillo, N. A., Neuta, Y., Lafaurie, G. I., Romero-Sánchez, C., & Castellanos, J. E. (2022). Purification of RgpA from external outer membrane vesicles of Porphyromonas gingivalis. Anaerobe, 77, 102647. https://doi.org/10.1016/j.anaerobe.2022.102647spa
dc.relation.referencesCastillo, Y., Castellanos, J. E., Lafaurie, G. I., & Castillo, D. M. (2022). Porphyromonas gingivalis outer membrane vesicles modulate cytokine and chemokine production by gingipain-dependent mechanisms in human macrophages. Archives of oral biology, 140, 105453. https://doi.org/10.1016/j.archoralbio.2022.105453spa
dc.relation.referencesCiesielski, O., Biesiekierska, M., Panthu, B., Soszyński, M., Pirola, L., & Balcerczyk, A. (2022). Citrullination in the pathology of inflammatory and autoimmune disorders: recent advances and future perspectives. Cellular and molecular life sciences: CMLS, 79(2), 94. https://doi.org/10.1007/s00018-022-04126-3spa
dc.relation.referencesChang, K., Yang, S. M., Kim, S. H., Han, K. H., Park, S. J., & Shin, J. I. (2014). Smoking and rheumatoid arthritis. International journal of molecular sciences, 15(12), 22279–22295. https://doi.org/10.3390/ijms151222279spa
dc.relation.referencesChaparro-Sanabria, J. A., Bautista-Molano, W., Bello-Gualtero, J. M., Chila-Moreno, L., Castillo, D. M., Valle-Oñate, R., Chalem, P., & Romero-Sánchez, C. (2019). Association of adipokines with rheumatic disease activity indexes and periodontal disease in patients with early rheumatoid arthritis and their first-degree relatives. International journal of rheumatic diseases, 22(11), 1990–2000. https://doi.org/10.1111/1756-185X.13724spa
dc.relation.referencesChapple, I., Mealey, B. L., Van Dyke, T. E., Bartold, P. M., Dommisch, H., Eickholz, P., Geisinger, M. L., Genco, R. J., Glogauer, M., Goldstein, M., Griffin, T. J., Holmstrup, P., Johnson, G. K., Kapila, Y., Lang, N. P., Meyle, J., Murakami, S., Plemons, J., Romito, G. A., Shapira, L., … Yoshie, H. (2018). Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. Journal of periodontology, 89 Suppl 1, S74–S84. https://doi.org/10.1002/JPER.17-0719spa
dc.relation.referencesChen, Y. W., Nagasawa, T., Wara-Aswapati, N., Ushida, Y., Wang, D., Takeuchi, Y., Kobayashi, H., Umeda, M., Inoue, Y., Iwai, T., Ishikawa, I., & Izumi, Y. (2009). Association between periodontitis and anti-cardiolipin antibodies in Buerger disease. Journal of clinical periodontology, 36(10), 830–835. https://doi.org/10.1111/j.1600-051X.2009.01467.xspa
dc.relation.referencesChen, Z., Potempa, J., Polanowski, A., Wikstrom, M., & Travis, J. (1992). Purification and characterization of a 50-kDa cysteine proteinase (gingipain) from Porphyromonas gingivalis. The Journal of biological chemistry, 267(26), 18896–18901.spa
dc.relation.referencesChiang, H. I., Li, J. R., Liu, C. C., Liu, P. Y., Chen, H. H., Chen, Y. M., Lan, J. L., & Chen, D. Y. (2019). An Association of Gut Microbiota with Different Phenotypes in Chinese Patients with Rheumatoid Arthritis. Journal of clinical medicine, 8(11), 1770. https://doi.org/10.3390/jcm8111770spa
dc.relation.referencesChou, Y. Y., Lai, K. L., Chen, D. Y., Lin, C. H., & Chen, H. H. (2015). Rheumatoid Arthritis Risk Associated with Periodontitis Exposure: A Nationwide, Population-Based Cohort Study. PloS one, 10(10), e0139693. https://doi.org/10.1371/journal.pone.0139693spa
dc.relation.referencesCrowe, J., Masone, B. S., & Ribbe, J. (1995). One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin. Molecular biotechnology, 4(3), 247–258. https://doi.org/10.1007/BF02779018spa
dc.relation.referencesCush J. J. (2021). Rheumatoid Arthritis: Early Diagnosis and Treatment. The Medical clinics of North America, 105(2), 355–365. https://doi.org/10.1016/j.mcna.2020.10.006spa
dc.relation.referencesCurtis, M. A., Kuramitsu, H. K., Lantz, M., Macrina, F. L., Nakayama, K., Potempa, J., Reynolds, E. C., & Aduse-Opoku, J. (1999). Molecular genetics and nomenclature of proteases of Porphyromonas gingivalis. Journal of periodontal research, 34(8), 464–472. https://doi.org/10.1111/j.1600-0765.1999.tb02282.xspa
dc.relation.referencesDarrah, E., & Andrade, F. (2018). Rheumatoid arthritis and citrullination. Current opinion in rheumatology, 30(1), 72–78. https://doi.org/10.1097/BOR.0000000000000452spa
dc.relation.referencesDashper, S. G., Mitchell, H. L., Seers, C. A., Gladman, S. L., Seemann, T., Bulach, D. M., Chandry, P. S., Cross, K. J., Cleal, S. M., & Reynolds, E. C. (2017). Porphyromonas gingivalisUses Specific Domain Rearrangements and Allelic Exchange to Generate Diversity in Surface Virulence Factors. Frontiers in microbiology, 8, 48. https://doi.org/10.3389/fmicb.2017.00048spa
dc.relation.referencesDelgado-Angulo, E. K., Bernabé, E., & Marcenes, W. (2016). Ethnic inequalities in periodontal disease among British adults. Journal of clinical periodontology, 43(11), 926–933. https://doi.org/10.1111/jcpe.12605spa
dc.relation.referencesDelgado-Vega, A. M., Martín, J., Granados, J., & Anaya, J. M. (2006). Epidemiología genética de la artritis reumatoide: qué esperar de América Latina? Biomedica: revista del Instituto Nacional de Salud, 26(4), 562–584spa
dc.relation.referencesde Pablo, P., Dietrich, T., & McAlindon, T. E. (2008). Association of periodontal disease and tooth loss with rheumatoid arthritis in the US population. The Journal of rheumatology, 35(1), 70–76.spa
dc.relation.referencesde Smit, M., van de Stadt, L. A., Janssen, K. M., Doornbos-van der Meer, B., Vissink, A., van Winkelhoff, A. J., Brouwer, E., Westra, J., & van Schaardenburg, D. (2014). Antibodies against Porphyromonas gingivalis in seropositive arthralgia patients do not predict development of rheumatoid arthritis. Annals of the rheumatic diseases, 73(6), 1277–1279. https://doi.org/10.1136/annrheumdis-2013-204594spa
dc.relation.referencesde Vries, C., Ruacho, G., Kindstedt, E., Potempa, B. A., Potempa, J., Klinge, B., Lundberg, P., Svenungsson, E., & Lundberg, K. (2022). Antibodies to Porphyromonas gingivalis Are Increased in Patients with Severe Periodontitis, and Associate with Presence of Specific Autoantibodies and Myocardial Infarction. Journal of clinical medicine, 11(4), 1008. https://doi.org/10.3390/jcm11041008spa
dc.relation.referencesDerksen, V., Huizinga, T., & van der Woude, D. (2017). The role of autoantibodies in the pathophysiology of rheumatoid arthritis. Seminars in immunopathology, 39(4), 437–446. https://doi.org/10.1007/s00281-017-0627-zspa
dc.relation.referencesDominy, S. S., Lynch, C., Ermini, F., Benedyk, M., Marczyk, A., Konradi, A., Nguyen, M., Haditsch, U., Raha, D., Griffin, C., Holsinger, L. J., Arastu-Kapur, S., Kaba, S., Lee, A., Ryder, M. I., Potempa, B., Mydel, P., Hellvard, A., Adamowicz, K., Hasturk, H., … Potempa, J. (2019). Porphyromonas gingivalis in Alzheimer's disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Science advances, 5(1), eaau3333. https://doi.org/10.1126/sciadv.aau3333spa
dc.relation.referencesEke, P. I., Page, R. C., Wei, L., Thornton-Evans, G., & Genco, R. J. (2012). Update of the case definitions for population-based surveillance of periodontitis. Journal of periodontology, 83(12), 1449–1454. https://doi.org/10.1902/jop.2012.110664spa
dc.relation.referencesEke, P. I., Wei, L., Borgnakke, W. S., Thornton-Evans, G., Zhang, X., Lu, H., McGuire, L. C., & Genco, R. J. (2016). Periodontitis prevalence in adults ≥ 65 years of age, in the USA. Periodontology 2000, 72(1), 76–95. https://doi.org/10.1111/prd.12145spa
dc.relation.referencesEke, P. I., Thornton-Evans, G. O., Wei, L., Borgnakke, W. S., Dye, B. A., & Genco, R. J. (2018). Periodontitis in US Adults: National Health and Nutrition Examination Survey 2009-2014. Journal of the American Dental Association (1939), 149(7), 576–588.e6. https://doi.org/10.1016/j.adaj.2018.04.023spa
dc.relation.referencesEriksson, K., Fei, G., Lundmark, A., Benchimol, D., Lee, L., Hu, Y., Kats, A., Saevarsdottir, S., Catrina, A. I., Klinge, B., Andersson, A. F., Klareskog, L., Lundberg, K., Jansson, L., & Yucel-Lindberg, T. (2019). Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis. Journal of clinical medicine, 8(5), 630. https://doi.org/10.3390/jcm8050630spa
dc.relation.referencesFederici, M. M., Venkat, K., Bam, N., Patel, K., Dal Monte, P. R., Fernie, B., Hensley, P., Carr, S., Baldoni, J., Truneh, A., & Erickson, J. (2003). Detection and consequences of recombinant protein isoforms: implications for biological potency. Developments in biologicals, 113, 53–114.spa
dc.relation.referencesFirestein, G. S., Budd, R., Gabriel, S. E., O’Dell, J. R., & McInnes, I. B. Kelley’s Textbook of Rheumatology: Expert Consult Premium Edition. Elsevier Health Sciences; 9th Ed. Páginas 1059-1074; 2013. ISBN 978-1-4377-1738-9.spa
dc.relation.referencesFirestein, G. S., & McInnes, I. B. (2017). Immunopathogenesis of Rheumatoid Arthritis. Immunity, 46(2), 183–196. https://doi.org/10.1016/j.immuni.2017.02.006spa
dc.relation.referencesFisher, B. A., Cartwright, A. J., Quirke, A. M., de Pablo, P., Romaguera, D., Panico, S., Mattiello, A., Gavrila, D., Navarro, C., Sacerdote, C., Vineis, P., Tumino, R., Lappin, D. F., Apatzidou, D., Culshaw, S., Potempa, J., Michaud, D. S., Riboli, E., & Venables, P. J. (2015). Smoking, Porphyromonas gingivalis and the immune response to citrullinated autoantigens before the clinical onset of rheumatoid arthritis in a Southern European nested case-control study. BMC musculoskeletal disorders, 16, 331. https://doi.org/10.1186/s12891-015-0792-yspa
dc.relation.referencesFitzpatrick, R. E., Wijeyewickrema, L. C., & Pike, R. N. (2009). The gingipains: scissors and glue of the periodontal pathogen, Porphyromonas gingivalis. Future microbiology, 4(4), 471–487. https://doi.org/10.2217/fmb.09.18spa
dc.relation.referencesFransen J., Stucki G., van Riel PLCM. (2003). Rheumatoid arthritis measures: Disease Activity Score (DAS), Disease Activity Score-28 (DAS28), Rapid Assessment of Disease Activity in Rheumatology (RADAR), and Rheumatoid Arthritis Disease Activity Index (RADAI). Arthritis Rheum, 49 (5S), S214–224. https://doi.org/:10.1002/art.11407spa
dc.relation.referencesGabriel S. E. (2001). The epidemiology of rheumatoid arthritis. Rheumatic diseases clinics of North America, 27(2), 269–281. https://doi.org/10.1016/s0889-857x(05)70201-5spa
dc.relation.referencesGabarrini, G., Chlebowicz, M. A., Vega Quiroz, M. E., Veloo, A., Rossen, J., Harmsen, H., Laine, M. L., van Dijl, J. M., & van Winkelhoff, A. J. (2018). Conserved Citrullinating Exoenzymes in Porphyromonas Species. Journal of dental research, 97(5), 556–562. https://doi.org/10.1177/0022034517747575spa
dc.relation.referencesGarcía, J., Santana, Z., Zumalacárregui, L., Quintana, M., González, D., Furrazola, G., Cruz, O. (2013). Estrategias de obtención de proteínas recombinantes en Escherichia coli. VacciMonitor, 22(2):30-39.spa
dc.relation.referencesGawron, K., Bereta, G., Nowakowska, Z., Lazarz-Bartyzel, K., Lazarz, M., Szmigielski, B., Mizgalska, D., Buda, A., Koziel, J., Oruba, Z., Chomyszyn-Gajewska, M., & Potempa, J. (2014). Peptidylarginine deiminase from Porphyromonas gingivalis contributes to infection of gingival fibroblasts and induction of prostaglandin E2 -signaling pathway. Molecular oral microbiology, 29(6), 321–332. https://doi.org/10.1111/omi.12081spa
dc.relation.referencesGhayoumi, N., Chen, C., & Slots, J. (2002). Dialister pneumosintes, a new putative periodontal pathogen. Journal of periodontal research, 37(1), 75–78. https://doi.org/10.1034/j.1600-0765.2002.05019.xspa
dc.relation.referencesGiles, J. T., Fert-Bober, J., Park, J. K., Bingham, C. O., 3rd, Andrade, F., Fox-Talbot, K., Pappas, D., Rosen, A., van Eyk, J., Bathon, J. M., & Halushka, M. K. (2012). Myocardial citrullination in rheumatoid arthritis: a correlative histopathologic study. Arthritis research & therapy, 14(1), R39. https://doi.org/10.1186/ar3752spa
dc.relation.referencesGómez-Bañuelos, E., Mukherjee, A., Darrah, E., & Andrade, F. (2019). Rheumatoid Arthritis-Associated Mechanisms of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Journal of clinical medicine, 8(9), 1309. https://doi.org/10.3390/jcm8091309spa
dc.relation.referencesGorasia, D. G., Lunar Silva, I., Butler, C. A., Chabalier, M., Doan, T., Cascales, E., Veith, P. D., & Reynolds, E. C. (2022). Protein Interactome Analysis of the Type IX Secretion System Identifies PorW as the Missing Link between the PorK/N Ring Complex and the Sov Translocon. Microbiology spectrum, 10(1), e0160221. https://doi.org/10.1128/spectrum.01602-21spa
dc.relation.referencesGorasia, D. G., Veith, P. D., Chen, D., Seers, C. A., Mitchell, H. A., Chen, Y. Y., Glew, M. D., Dashper, S. G., & Reynolds, E. C. (2015). Porphyromonas gingivalis Type IX Secretion Substrates Are Cleaved and Modified by a Sortase-Like Mechanism. PLoS pathogens, 11(9), e1005152. https://doi.org/10.1371/journal.ppat.1005152spa
dc.relation.referencesGoulas, T., Mizgalska, D., Garcia-Ferrer, I., Kantyka, T., Guevara, T., Szmigielski, B., Sroka, A., Millán, C., Usón, I., Veillard, F., Potempa, B., Mydel, P., Solà, M., Potempa, J., & Gomis-Rüth, F. X. (2015). Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase. Scientific reports, 5, 11969. https://doi.org/10.1038/srep11969spa
dc.relation.referencesGraves D. (2008). Cytokines that promote periodontal tissue destruction. Journal of periodontology, 79(8 Suppl), 1585–1591. https://doi.org/10.1902/jop.2008.080183spa
dc.relation.referencesGuevara, T., Rodríguez-Banqueri, A., Lasica, A. M., Ksiazek, M., Potempa, B. A., Potempa, J., & Gomis-Rüth, F. X. (2019). Structural determinants of inhibition of Porphyromonas gingivalis gingipain K by KYT-36, a potent, selective, and bioavailable peptidase inhibitor. Scientific reports, 9(1), 4935. https://doi.org/10.1038/s41598-019-41354-3spa
dc.relation.referencesGully, N., Bright, R., Marino, V., Marchant, C., Cantley, M., Haynes, D., Butler, C., Dashper, S., Reynolds, E., & Bartold, M. (2014). Porphyromonas gingivalis peptidylarginine deiminase, a key contributor in the pathogenesis of experimental periodontal disease and experimental arthritis. PloS one, 9(6), e100838. https://doi.org/10.1371/journal.pone.0100838spa
dc.relation.referencesHajishengallis G. (2015). Periodontitis: from microbial immune subversion to systemic inflammation. Nature reviews. Immunology, 15(1), 30–44. https://doi.org/10.1038/nri3785spa
dc.relation.referencesHajishengallis, G., Darveau, R. P., & Curtis, M. A. (2012). The keystone-pathogen hypothesis. Nature reviews. Microbiology, 10(10), 717–725. https://doi.org/10.1038/nrmicro2873spa
dc.relation.referencesHamamoto, Y., Ouhara, K., Munenaga, S., Shoji, M., Ozawa, T., Hisatsune, J., Kado, I., Kajiya, M., Matsuda, S., Kawai, T., Mizuno, N., Fujita, T., Hirata, S., Tanimoto, K., Nakayama, K., Kishi, H., Sugiyama, E., & Kurihara, H. (2020). Effect of Porphyromonas gingivalis infection on gut dysbiosis and resultant arthritis exacerbation in mouse model. Arthritis research & therapy, 22(1), 249. https://doi.org/10.1186/s13075-020-02348-zspa
dc.relation.referencesHedström, A. K., Stawiarz, L., Klareskog, L., & Alfredsson, L. (2018). Smoking and susceptibility to rheumatoid arthritis in a Swedish population-based case-control study. European journal of epidemiology, 33(4), 415–423. https://doi.org/10.1007/s10654-018-0360-5spa
dc.relation.referencesHeredia-P, A. M., Lafaurie, G. I., Bautista-Molano, W., Trujillo, T. G., Chalem-Choueka, P., Bello-Gualtero, J. M., Pacheco-Tena, C., Chila-Moreno, L., & Romero-Sánchez, C. (2019). Predictive factors related to the progression of periodontal disease in patients with early rheumatoid arthritis: a cohort study. BMC oral health, 19(1), 240. https://doi.org/10.1186/s12903-019-0939-6spa
dc.relation.referencesHirai, K., Yamaguchi-Tomikawa, T., Eguchi, T., Maeda, H., & Takashiba, S. (2020). Identification and Modification of Porphyromonas gingivalis Cysteine Protease, Gingipain, Ideal for Screening Periodontitis. Frontiers in immunology, 11, 1017. https://doi.org/10.3389/fimmu.2020.01017spa
dc.relation.referencesHitchon, C. A., Chandad, F., Ferucci, E. D., Willemze, A., Ioan-Facsinay, A., van der Woude, D., Markland, J., Robinson, D., Elias, B., Newkirk, M., Toes, R. M., Huizinga, T. W., & El-Gabalawy, H. S. (2010). Antibodies to Porphyromonas gingivalis are associated with anticitrullinated protein antibodies in patients with rheumatoid arthritis and their relatives. The Journal of rheumatology, 37(6), 1105–1112. https://doi.org/10.3899/jrheum.091323spa
dc.relation.referencesHuck, O., You, J., Han, X., Cai, B., Panek, J., & Amar, S. (2018). Reduction of Articular and Systemic Inflammation by Kava-241 in a Porphyromonas gingivalis-Induced Arthritis Murine Model. Infection and immunity, 86(9), e00356-18. https://doi.org/10.1128/IAI.00356-18spa
dc.relation.referencesImamura T. (2003)a. The role of gingipains in the pathogenesis of periodontal disease. Journal of periodontology, 74(1), 111–118. https://doi.org/10.1902/jop.2003.74.1.111spa
dc.relation.referencesImamura, T., Travis, J., & Potempa, J. (2003)b. The biphasic virulence activities of gingipains: activation and inactivation of host proteins. Current protein & peptide science, 4(6), 443–450. https://doi.org/10.2174/1389203033487027spa
dc.relation.referencesIngalagi, P., Bhat, K. G., Kulkarni, R. D., Kotrashetti, V. S., Kumbar, V., & Kugaji, M. (2022). Detection and comparison of prevalence of Porphyromonas gingivalis through culture and Real Time-polymerase chain reaction in subgingival plaque samples of chronic periodontitis and healthy individuals. Journal of oral and maxillofacial pathology: JOMFP, 26(2), 288. https://doi.org/10.4103/jomfp.jomfp_163_21spa
dc.relation.referencesJang, S., Kwon, E. J., & Lee, J. J. (2022). Rheumatoid Arthritis: Pathogenic Roles of Diverse Immune Cells. International journal of molecular sciences, 23(2), 905. https://doi.org/10.3390/ijms23020905spa
dc.relation.referencesJasemi, S., Erre, G. L., Cadoni, M. L., Bo, M., & Sechi, L. A. (2021). Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients. Journal of clinical medicine, 10(21), 5153. https://doi.org/10.3390/jcm10215153spa
dc.relation.referencesJenning, M., Marklein, B., Ytterberg, J., Zubarev, R. A., Joshua, V., van Schaardenburg, D., van de Stadt, L., Catrina, A. I., Nonhoff, U., Häupl, T., Konthur, Z., Burmester, G. R., & Skriner, K. (2020). Bacterial citrullinated epitopes generated by Porphyromonas gingivalis infection-a missing link for ACPA production. Annals of the rheumatic diseases, 79(9), 1194–1202. https://doi.org/10.1136/annrheumdis-2019-216919spa
dc.relation.referencesJeong, H., Kim, H. J., & Lee, S. J. (2015). Complete Genome Sequence of Escherichia coli Strain BL21. Genome announcements, 3(2), e00134-15. https://doi.org/10.1128/genomeA.00134-15spa
dc.relation.referencesJia, L., Han, N., Du, J., Guo, L., Luo, Z., & Liu, Y. (2019). Pathogenesis of Important Virulence Factors of Porphyromonas gingivalis via Toll-Like Receptors. Frontiers in cellular and infection microbiology, 9, 262. https://doi.org/10.3389/fcimb.2019.00262spa
dc.relation.referencesJohansson, L., Sherina, N., Kharlamova, N., Potempa, B., Larsson, B., Israelsson, L., Potempa, J., Rantapää-Dahlqvist, S., & Lundberg, K. (2016). Concentration of antibodies against Porphyromonas gingivalis increased before the onset of symptoms of rheumatoid arthritis. Arthritis research & therapy, 18(1), 201. https://doi.org/10.1186/s13075-016-1100-4spa
dc.relation.referencesJung, H., Jung, S. M., Rim, Y. A., Park, N., Nam, Y., Lee, J., Park, S. H., & Ju, J. H. (2017). Arthritic role of Porphyromonas gingivalis in collagen-induced arthritis mice. PloS one, 12(11), e0188698. https://doi.org/10.1371/journal.pone.0188698spa
dc.relation.referencesKadowaki, T., Yoneda, M., Okamoto, K., Maeda, K., & Yamamoto, K. (1994). Purification and characterization of a novel arginine-specific cysteine proteinase (argingipain) involved in the pathogenesis of periodontal disease from the culture supernatant of Porphyromonas gingivalis. The Journal of biological chemistry, 269(33), 21371–21378.spa
dc.relation.referencesKharlamova, N., Jiang, X., Sherina, N., Potempa, B., Israelsson, L., Quirke, A. M., Eriksson, K., Yucel-Lindberg, T., Venables, P. J., Potempa, J., Alfredsson, L., & Lundberg, K. (2016). Antibodies to Porphyromonas gingivalis Indicate Interaction Between Oral Infection, Smoking, and Risk Genes in Rheumatoid Arthritis Etiology. Arthritis & rheumatology (Hoboken, N.J.), 68(3), 604–613. https://doi.org/10.1002/art.39491spa
dc.relation.referencesKlareskog, L., Widhe, M., Hermansson, M., & Rönnelid, J. (2008). Antibodies to citrullinated proteins in arthritis: pathology and promise. Current opinion in rheumatology, 20(3), 300–305. https://doi.org/10.1097/BOR.0b013e3282fbd22aspa
dc.relation.referencesKonig, M. F., Paracha, A. S., Moni, M., Bingham, C. O., 3rd, & Andrade, F. (2015). Defining the role of Porphyromonas gingivalis peptidylarginine deiminase (PPAD) in rheumatoid arthritis through the study of PPAD biology. Annals of the rheumatic diseases, 74(11), 2054–2061. https://doi.org/10.1136/annrheumdis-2014-205385spa
dc.relation.referencesKwon, T., Lamster, I. B., & Levin, L. (2021). Current Concepts in the Management of Periodontitis. International dental journal, 71(6), 462–476. https://doi.org/10.1111/idj.12630spa
dc.relation.referencesKuramitsu H. K. (1998). Proteases of Porphyromonas gingivalis: what don't they do?. Oral microbiology and immunology, 13(5), 263–270. https://doi.org/10.1111/j.1399-302x.1998.tb00706.xspa
dc.relation.referencesKrutyhołowa, A., Strzelec, K., Dziedzic, A., Bereta, G. P., Łazarz-Bartyzel, K., Potempa, J., & Gawron, K. (2022). Host and bacterial factors linking periodontitis and rheumatoid arthritis. Frontiers in immunology, 13, 980805. https://doi.org/10.3389/fimmu.2022.980805spa
dc.relation.referencesLafaurie, G. I., Neuta, Y., Ríos, R., Pacheco-Montealegre, M., Pianeta, R., Castillo, D. M., Herrera, D., Reyes, J., Diaz, L., Castillo, Y., Sanz, M., & Iniesta, M. (2022). Differences in the subgingival microbiome according to stage of periodontitis: A comparison of two geographic regions. PloS one, 17(8), e0273523. https://doi.org/10.1371/journal.pone.0273523spa
dc.relation.referencesLafaurie, G.I., Castillo, D.M., Iniesta, M., Sanz, M., Gómez, L. A., Castillo, Y., Pianeta, R., Delgadillo, N.A., Neuta, Y., Diaz-Báez, D., Herrera, D. (2023). Differential analysis of culturable and unculturable subgingival target microorganisms according to the stages of periodontitis. Clinical Oral Investigations (Aceptado para publicación).spa
dc.relation.referencesLafaurie, G. I., Mayorga-Fayad, I., Torres, M. F., Castillo, D. M., Aya, M. R., Barón, A., & Hurtado, P. A. (2007a). Periodontopathic microorganisms in peripheric blood after scaling and root planing. Journal of clinical periodontology, 34(10), 873–879. https://doi.org/10.1111/j.1600-051X.2007.01125.xspa
dc.relation.referencesLafaurie, G. I., Contreras, A., Barón, A., Botero, J., Mayorga-Fayad, I., Jaramillo, A., Giraldo, A., González, F., Mantilla, S., Botero, A., Archila, L. H., Díaz, A., Chacón, T., Castillo, D. M., Betancourt, M., Del Rosario Aya, M., & Arce, R. (2007b). Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. Journal of periodontology, 78(4), 629–639. https://doi.org/10.1902/jop.2007.060187spa
dc.relation.referencesLaugisch, O., Wong, A., Sroka, A., Kantyka, T., Koziel, J., Neuhaus, K., Sculean, A., Venables, P. J., Potempa, J., Möller, B., & Eick, S. (2016). Citrullination in the periodontium--a possible link between periodontitis and rheumatoid arthritis. Clinical oral investigations, 20(4), 675–683. https://doi.org/10.1007/s00784-015-1556-7spa
dc.relation.referencesLee, J. Y., Choi, I. A., Kim, J. H., Kim, K. H., Lee, E. Y., Lee, E. B., Lee, Y. M., & Song, Y. W. (2015). Association between anti-Porphyromonas gingivalis or anti-α-enolase antibody and severity of periodontitis or rheumatoid arthritis (RA) disease activity in RA. BMC musculoskeletal disorders, 16, 190. https://doi.org/10.1186/s12891-015-0647-6spa
dc.relation.referencesLeech, M. T., & Bartold, P. M. (2015). The association between rheumatoid arthritis and periodontitis. Best practice & research. Clinical rheumatology, 29(2), 189–201. https://doi.org/10.1016/j.berh.2015.03.001spa
dc.relation.referencesLi, Y., Guo, R., Oduro, P. K., Sun, T., Chen, H., Yi, Y., Zeng, W., Wang, Q., Leng, L., Yang, L., & Zhang, J. (2022). The Relationship Between Porphyromonas gingivalis and Rheumatoid Arthritis: A Meta-Analysis. Frontiers in cellular and infection microbiology, 12, 956417. https://doi.org/10.3389/fcimb.2022.956417spa
dc.relation.referencesLi, N., & Collyer, C. A. (2011). Gingipains from Porphyromonas gingivalis- Complex domain structures confer diverse functions. European journal of microbiology & immunology, 1(1), 41–58. https://doi.org/10.1556/EuJMI.1.2011.1.7spa
dc.relation.referencesLiu, X., Zou, Q., Zeng, B., Fang, Y., & Wei, H. (2013). Analysis of fecal Lactobacillus community structure in patients with early rheumatoid arthritis. Current microbiology, 67(2), 170–176. https://doi.org/10.1007/s00284-013-0338-1spa
dc.relation.referencesLoutan, L., Alpizar-Rodriguez, D., Courvoisier, D. S., Finckh, A., Mombelli, A., & Giannopoulou, C. (2019). Periodontal status correlates with anti-citrullinated protein antibodies in first-degree relatives of individuals with rheumatoid arthritis. Journal of clinical periodontology, 46(7), 690–698. https://doi.org/10.1111/jcpe.13117spa
dc.relation.referencesLübcke, P. M., Ebbers, M., Volzke, J., Bull, J., Kneitz, S., Engelmann, R., Lang, H., Kreikemeyer, B., & Müller-Hilke, B. (2019). Periodontal treatment prevents arthritis in mice and methotrexate ameliorates periodontal bone loss. Scientific reports, 9(1), 8128. https://doi.org/10.1038/s41598-019-44512-9spa
dc.relation.referencesMa, K. S., Chiang, C. H., Chen, Y. W., & Wang, L. T. (2021). Correspondence to 'Bacterial citrullinated epitopes generated by Porphyromonas gingivalis infection-a missing link for ACPA production'. Annals of the rheumatic diseases, annrheumdis-2020-219255. Advance online publication. https://doi.org/10.1136/annrheumdis-2020-219255spa
dc.relation.referencesMangat, P., Wegner, N., Venables, P. J., & Potempa, J. (2010). Bacterial and human peptidylarginine deiminases: targets for inhibiting the autoimmune response in rheumatoid arthritis?. Arthritis research & therapy, 12(3), 209. https://doi.org/10.1186/ar3000spa
dc.relation.referencesMargetts, M. B., Barr, I. G., & Webb, E. A. (2000). Overexpression, purification, and refolding of a Porphyromonas gingivalis cysteine protease from Escherichia coli. Protein expression and purification, 18(3), 262–268. https://doi.org/10.1006/prep.2000.1193spa
dc.relation.referencesMantri, C. K., Chen, C. H., Dong, X., Goodwin, J. S., Pratap, S., Paromov, V., & Xie, H. (2015). Fimbriae-mediated outer membrane vesicle production and invasion of Porphyromonas gingivalis. MicrobiologyOpen, 4(1), 53–65. https://doi.org/10.1002/mbo3.221spa
dc.relation.referencesMarchesan, J. T., Gerow, E. A., Schaff, R., Taut, A. D., Shin, S. Y., Sugai, J., Brand, D., Burberry, A., Jorns, J., Lundy, S. K., Nuñez, G., Fox, D. A., & Giannobile, W. V. (2013). Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis. Arthritis research & therapy, 15(6), R186. https://doi.org/10.1186/ar4376spa
dc.relation.referencesMaresz, K. J., Hellvard, A., Sroka, A., Adamowicz, K., Bielecka, E., Koziel, J., Gawron, K., Mizgalska, D., Marcinska, K. A., Benedyk, M., Pyrc, K., Quirke, A. M., Jonsson, R., Alzabin, S., Venables, P. J., Nguyen, K. A., Mydel, P., & Potempa, J. (2013). Porphyromonas gingivalis facilitates the development and progression of destructive arthritis through its unique bacterial peptidylarginine deiminase (PAD). PLoS pathogens, 9(9), e1003627. https://doi.org/10.1371/journal.ppat.1003627spa
dc.relation.referencesMartínez-Rivera, J. I., Xibillé-Friedmann, D. X., González-Christen, J., de la Garza-Ramos, M. A., Carrillo-Vázquez, S. M., & Montiel-Hernández, J. L. (2017). Salivary ammonia levels and Tannerella forsythia are associated with rheumatoid arthritis: A cross sectional study. Clinical and experimental dental research, 3(3), 107–114. https://doi.org/10.1002/cre2.68spa
dc.relation.referencesMayorga-Fayad, I., Lafaurie, G. I., Contreras, A., Castillo, D. M., Barón, A., & Aya, M. (2007). Microflora subgingival en periodontitis crónica y agresiva en Bogotá, Colombia: un acercamiento epidemiológico. Biomedica : revista del Instituto Nacional de Salud, 27(1), 21–33.spa
dc.relation.referencesMcGraw, W. T., Potempa, J., Farley, D., & Travis, J. (1999). Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis, peptidylarginine deiminase. Infection and immunity, 67(7), 3248–3256. https://doi.org/10.1128/IAI.67.7.3248-3256.1999spa
dc.relation.referencesMinisterio de salud. Estudio Nacional En Salud Bucal (ENSAB IV). Bogotá, Colombia. 2014;4.Consultado en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ENSAB-IV-Situacion-Bucal-Actual.pdfspa
dc.relation.referencesMikuls, T. R., Payne, J. B., Yu, F., Thiele, G. M., Reynolds, R. J., Cannon, G. W., Markt, J., McGowan, D., Kerr, G. S., Redman, R. S., Reimold, A., Griffiths, G., Beatty, M., Gonzalez, S. M., Bergman, D. A., Hamilton, B. C., 3rd, Erickson, A. R., Sokolove, J., Robinson, W. H., Walker, C., … O'Dell, J. R. (2014). Periodontitis and Porphyromonas gingivalis in patients with rheumatoid arthritis. Arthritis & rheumatology (Hoboken, N.J.), 66(5), 1090–1100. https://doi.org/10.1002/art.38348spa
dc.relation.referencesMontgomery, A. B., Kopec, J., Shrestha, L., Thezenas, M. L., Burgess-Brown, N. A., Fischer, R., Yue, W. W., & Venables, P. J. (2016). Crystal structure of Porphyromonas gingivalis peptidylarginine deiminase: implications for autoimmunity in rheumatoid arthritis. Annals of the rheumatic diseases, 75(6), 1255–1261. https://doi.org/10.1136/annrheumdis-2015-207656spa
dc.relation.referencesMoen, K., Brun, J. G., Madland, T. M., Tynning, T., & Jonsson, R. (2003). Immunoglobulin G and A antibody responses to Bacteroides forsythus and Prevotella intermedia in sera and synovial fluids of arthritis patients. Clinical and diagnostic laboratory immunology, 10(6), 1043–1050. https://doi.org/10.1128/cdli.10.6.1043-1050.2003spa
dc.relation.referencesMorales, L., Velandia, M. L., Calderon, M. A., Castellanos, J. E., & Chaparro-Olaya, J. (2017). Anticuerpos policlonales contra la proteína recombinante NS3 del virus del dengue. Biomedica: revista del Instituto Nacional de Salud, 37(1), 131–140. https://doi.org/10.7705/biomedica.v37i1.3249spa
dc.relation.referencesMoses, T., & Holland, P. W. (2010). A comparison of statistical selection strategies for univariate and bivariate log-linear models. The British journal of mathematical and statistical psychology, 63(Pt 3), 557–574. https://doi.org/10.1348/000711009X478580spa
dc.relation.referencesMuñoz-Atienza, E., Flak, M. B., Sirr, J., Paramonov, N. A., Aduse-Opoku, J., Pitzalis, C., & Curtis, M. A. (2020). The P. gingivalis Autocitrullinome Is Not a Target for ACPA in Early Rheumatoid Arthritis. Journal of dental research, 99(4), 456–462. https://doi.org/10.1177/0022034519898144spa
dc.relation.referencesMunenaga, S., Ouhara, K., Hamamoto, Y., Kajiya, M., Takeda, K., Yamasaki, S., Kawai, T., Mizuno, N., Fujita, T., Sugiyama, E., & Kurihara, H. (2018). The involvement of C5a in the progression of experimental arthritis with Porphyromonas gingivalis infection in SKG mice. Arthritis research & therapy, 20(1), 247. https://doi.org/10.1186/s13075-018-1744-3spa
dc.relation.referencesNakao, R., Takashiba, S., Kosono, S., Yoshida, M., Watanabe, H., Ohnishi, M., & Senpuku, H. (2014). Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses. Microbes and infection, 16(1), 6–16. https://doi.org/10.1016/j.micinf.2013.10.005spa
dc.relation.referencesNakayama, K., Kadowaki, T., Okamoto, K., & Yamamoto, K. (1995). Construction and characterization of arginine-specific cysteine proteinase (Arg-gingipain)-deficient mutants of Porphyromonas gingivalis. Evidence for significant contribution of Arg-gingipain to virulence. The Journal of biological chemistry, 270(40), 23619–23626. https://doi.org/10.1074/jbc.270.40.23619spa
dc.relation.referencesNemoto, T. K., & Ohara-Nemoto, Y. (2016). Exopeptidases and gingipains in Porphyromonas gingivalis as prerequisites for its amino acid metabolism. The Japanese dental science review, 52(1), 22–29. https://doi.org/10.1016/j.jdsr.2015.08.002spa
dc.relation.referencesNunes, J. M., Fillis, T., Page, M. J., Venter, C., Lancry, O., Kell, D. B., Windberger, U., & Pretorius, E. (2020). Gingipain R1 and Lipopolysaccharide From Porphyromonas gingivalis Have Major Effects on Blood Clot Morphology and Mechanics. Frontiers in immunology, 11, 1551. https://doi.org/10.3389/fimmu.2020.01551spa
dc.relation.referencesOkada, M., Kobayashi, T., Ito, S., Yokoyama, T., Abe, A., Murasawa, A., & Yoshie, H. (2013). Periodontal treatment decreases levels of antibodies to Porphyromonas gingivalis and citrulline in patients with rheumatoid arthritis and periodontitis. Journal of periodontology, 84(12), e74–e84. https://doi.org/10.1902/jop.2013.130079spa
dc.relation.referencesOkada, M., Kobayashi, T., Ito, S., Yokoyama, T., Komatsu, Y., Abe, A., Murasawa, A., & Yoshie, H. (2011). Antibody responses to periodontopathic bacteria in relation to rheumatoid arthritis in Japanese adults. Journal of periodontology, 82(10), 1433–1441. https://doi.org/10.1902/jop.2011.110020spa
dc.relation.referencesOlsen, I., Singhrao, S. K., & Potempa, J. (2018). Citrullination as a plausible link to periodontitis, rheumatoid arthritis, atherosclerosis and Alzheimer's disease. Journal of oral microbiology, 10(1), 1487742. https://doi.org/10.1080/20002297.2018.1487742spa
dc.relation.referencesOwczarek, B., Gerszberg, A., & Hnatuszko-Konka, K. (2019). A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals. BioMed research international, 2019, 4216060. https://doi.org/10.1155/2019/4216060spa
dc.relation.referencesPadyukov L. (2022). Genetics of rheumatoid arthritis. Seminars in immunopathology, 44(1), 47–62. https://doi.org/10.1007/s00281-022-00912-0spa
dc.relation.referencesPage, R. C., & Eke, P. I. (2007). Case definitions for use in population-based surveillance of periodontitis. Journal of periodontology, 78(7 Suppl), 1387–1399. https://doi.org/10.1902/jop.2007.060264spa
dc.relation.referencesPathirana, R. D., O'Brien-Simpson, N. M., Veith, P. D., Riley, P. F., & Reynolds, E. C. (2006). Characterization of proteinase-adhesin complexes of Porphyromonas gingivalis. Microbiology (Reading, England), 152(Pt 8), 2381–2394. https://doi.org/10.1099/mic.0.28787-0spa
dc.relation.referencesPathirana, R. D., O'Brien-Simpson, N. M., Brammar, G. C., Slakeski, N., & Reynolds, E. C. (2007). Kgp and RgpB, but not RgpA, are important for Porphyromonas gingivalis virulence in the murine periodontitis model. Infection and immunity, 75(3), 1436–1442. https://doi.org/10.1128/IAI.01627-06spa
dc.relation.referencesPianta, A., Arvikar, S., Strle, K., Drouin, E. E., Wang, Q., Costello, C. E., & Steere, A. C. (2017). Evidence of the Immune Relevance of Prevotella copri, a Gut Microbe, in Patients With Rheumatoid Arthritis. Arthritis & rheumatology (Hoboken, N.J.), 69(5), 964–975. https://doi.org/10.1002/art.40003spa
dc.relation.referencesPosada-López, A., Botero, J. E., Pineda-Tamayo, R. A., & Agudelo-Suárez, A. A. (2022). The Effect of Periodontal Treatment on Clinical and Biological Indicators, Quality of Life, and Oral Health in Rheumatoid Arthritis Patients: A Quasi-Experimental Study. International journal of environmental research and public health, 19(3), 1789. https://doi.org/10.3390/ijerph19031789spa
dc.relation.referencesPotempa, J., Mydel, P., & Koziel, J. (2017). The case for periodontitis in the pathogenesis of rheumatoid arthritis. Nature reviews. Rheumatology, 13(10), 606–620. https://doi.org/10.1038/nrrheum.2017.132spa
dc.relation.referencesPotempa J, Nguyen KA (2007). Purification and characterization of gingipains. Curr Protoc Protein Sci. Chapter 21.spa
dc.relation.referencesPotempa, J., Sroka, A., Imamura, T., & Travis, J. (2003). Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes. Current protein & peptide science, 4(6), 397–407. https://doi.org/10.2174/1389203033487036spa
dc.relation.referencesPotempa, J., Pavloff, N., & Travis, J. (1995)a. Porphyromonas gingivalis: a proteinase/gene accounting audit. Trends in microbiology, 3(11), 430–434. https://doi.org/10.1016/s0966-842x(00)88996-9spa
dc.relation.referencesPotempa, J., Pike, R., & Travis, J. (1995)b. The multiple forms of trypsin-like activity present in various strains of Porphyromonas gingivalis are due to the presence of either Arg-gingipain or Lys-gingipain. Infection and immunity, 63(4), 1176–1182. https://doi.org/10.1128/iai.63.4.1176-1182.1995spa
dc.relation.referencesPuszczewicz, M., & Iwaszkiewicz, C. (2011). Role of anti-citrullinated protein antibodies in diagnosis and prognosis of rheumatoid arthritis. Archives of medical science: AMS, 7(2), 189–194. https://doi.org/10.5114/aoms.2011.22067spa
dc.relation.referencesQiao, Y., Wang, Z., Li, Y., Han, Y., Zhou, Y., & Cao, X. (2020). Rheumatoid arthritis risk in periodontitis patients: A systematic review and meta-analysis. Joint bone spine, 87(6), 556–564. https://doi.org/10.1016/j.jbspin.2020.04.024spa
dc.relation.referencesQin, B., Yang, M., Fu, H., Ma, N., Wei, T., Tang, Q., Hu, Z., Liang, Y., Yang, Z., & Zhong, R. (2015). Body mass index and the risk of rheumatoid arthritis: a systematic review and dose-response meta-analysis. Arthritis research & therapy, 17(1), 86. https://doi.org/10.1186/s13075-015-0601-xspa
dc.relation.referencesQuirke, A. M., Lugli, E. B., Wegner, N., Hamilton, B. C., Charles, P., Chowdhury, M., Ytterberg, A. J., Zubarev, R. A., Potempa, J., Culshaw, S., Guo, Y., Fisher, B. A., Thiele, G., Mikuls, T. R., & Venables, P. J. (2014). Heightened immune response to autocitrullinated Porphyromonas gingivalis peptidylarginine deiminase: a potential mechanism for breaching immunologic tolerance in rheumatoid arthritis. Annals of the rheumatic diseases, 73(1), 263–269. https://doi.org/10.1136/annrheumdis-2012-202726spa
dc.relation.referencesRahajoe, P. S., de Smit, M., Schuurmans, G., Raveling-Eelsing, E., Kertia, N., Vissink, A., & Westra, J. (2020). Increased IgA anti-citrullinated protein antibodies in the periodontal inflammatory exudate of healthy individuals compared to rheumatoid arthritis patients. Journal of clinical periodontology, 47(5), 552–560. https://doi.org/10.1111/jcpe.13277spa
dc.relation.referencesRepeke, C. E., Cardoso, C. R., Claudino, M., Silveira, E. M., Trombone, A. P., Campanelli, A. P., Silva, J. S., Martins, W., Jr, & Garlet, G. P. (2012). Non-inflammatory destructive periodontal disease: a clinical, microbiological, immunological and genetic investigation. Journal of applied oral science : revista FOB, 20(1), 113–121. https://doi.org/10.1590/s1678-77572012000100020spa
dc.relation.referencesRichards D. (2014). Review finds that severe periodontitis affects 11% of the world population. Evidence-based dentistry, 15(3), 70–71. https://doi.org/10.1038/sj.ebd.6401037spa
dc.relation.referencesRodríguez, J., Lafaurie, G. I., Bautista-Molano, W., Chila-Moreno, L., Bello-Gualtero, J. M., & Romero-Sánchez, C. (2021). Adipokines and periodontal markers as risk indicators of early rheumatoid arthritis: a cross-sectional study. Clinical oral investigations, 25(4), 1685–1695. https://doi.org/10.1007/s00784-020-03469-0spa
dc.relation.referencesRodríguez, S. B., Stitt, B. L., & Ash, D. E. (2009). Expression of peptidylarginine deiminase from Porphyromonas gingivalis in Escherichia coli: enzyme purification and characterization. Archives of biochemistry and biophysics, 488(1), 14–22. https://doi.org/10.1016/j.abb.2009.06.010spa
dc.relation.referencesRöhner, E., Detert, J., Kolar, P., Hocke, A., N'Guessan, P., Matziolis, G., Kanitz, V., Bernimoulin, J. P., Kielbassa, A., Burmester, G. R., Buttgereit, F., & Pischon, N. (2010). Induced apoptosis of chondrocytes by Porphyromonas gingivalis as a possible pathway for cartilage loss in rheumatoid arthritis. Calcified tissue international, 87(4), 333–340. https://doi.org/10.1007/s00223-010-9389-5spa
dc.relation.referencesRomero-Sánchez, C., Rodríguez, C., Santos-Moreno, P., Mesa, A. M., Lafaurie, G. I., Giraldo-Q, S., De-Avila, J., Castillo, D. M., Duran, M., Chalem, P. C., Bello Gualtero, J. M., & Valle-Oñate, R. (2017). Is the Treatment with Biological or Non-biological DMARDS a Modifier of Periodontal Condition in Patients with Rheumatoid Arthritis?. Current rheumatology reviews, 13(2), 139–151. https://doi.org/10.2174/1573397113666170407161520spa
dc.relation.referencesRovas, A., Puriene, A., Punceviciene, E., Butrimiene, I., Stuopelyte, K., & Jarmalaite, S. (2021). Associations of periodontal status in periodontitis and rheumatoid arthritis patients. Journal of periodontal & implant science, 51(2), 124–134. https://doi.org/10.5051/jpis.2006060303spa
dc.relation.referencesSato, K., Takahashi, N., Kato, T., Matsuda, Y., Yokoji, M., Yamada, M., Nakajima, T., Kondo, N., Endo, N., Yamamoto, R., Noiri, Y., Ohno, H., & Yamazaki, K. (2017). Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system. Scientific reports, 7(1), 6955. https://doi.org/10.1038/s41598-017-07196-7spa
dc.relation.referencesSchenkein, H. A., Bradley, J. L., & Purkall, D. B. (2013). Anticardiolipin in Porphyromonas gingivalis antisera causes fetal loss in mice. Journal of dental research, 92(9), 814–818. https://doi.org/10.1177/0022034513497959spa
dc.relation.referencesSchmalz, G., Noack, S., Patschan, S., Patschan, D., Müller, G. A., Rupprecht, A., Schmickler, J., Haak, R., & Ziebolz, D. (2020). Disease activity, morning stiffness and missing teeth are associated with oral health-related quality of life in individuals with rheumatoid arthritis. Clinical oral investigations, 24(10), 3559–3566. https://doi.org/10.1007/s00784-020-03226-3spa
dc.relation.referencesSchmickler, J., Rupprecht, A., Patschan, S., Patschan, D., Müller, G. A., Haak, R., Mausberg, R. F., Schmalz, G., Kottmann, T., & Ziebolz, D. (2017). Cross-Sectional Evaluation of Periodontal Status and Microbiologic and Rheumatoid Parameters in a Large Cohort of Patients With Rheumatoid Arthritis. Journal of periodontology, 88(4), 368–379. https://doi.org/10.1902/jop.2016.160355spa
dc.relation.referencesSeers, C. A., Mahmud, A., Huq, N. L., Cross, K. J., & Reynolds, E. C. (2020). Porphyromonas gingivalis laboratory strains and clinical isolates exhibit different distribution of cell surface and secreted gingipains. Journal of oral microbiology, 13(1), 1858001. https://doi.org/10.1080/20002297.2020.1858001spa
dc.relation.referencesSerrano, C., & Suarez, E. (2019). Prevalence of Severe Periodontitis in a Colombian Adult Population. Journal of the International Academy of Periodontology, 21(2), 53–62.spa
dc.relation.referencesShimada, A., Kobayashi, T., Ito, S., Okada, M., Murasawa, A., Nakazono, K., & Yoshie, H. (2016). Expression of anti-Porphyromonas gingivalis peptidylarginine deiminase immunoglobulin G and peptidylarginine deiminase-4 in patients with rheumatoid arthritis and periodontitis. Journal of periodontal research, 51(1), 103–111. https://doi.org/10.1111/jre.12288spa
dc.relation.referencesSippert, E. Â., de Oliveira e Silva, C., Ayo, C. M., Marques, S. B., Visentainer, J. E., & Sell, A. M. (2015). HLA Haplotypes and Genotypes Frequencies in Brazilian Chronic Periodontitis Patients. Mediators of inflammation, 2015, 481656. https://doi.org/10.1155/2015/481656spa
dc.relation.referencesSlots J. (1987). Detection of colonies of Bacteroides gingivalis by a rapid fluorescence assay for trypsin-like activity. Oral microbiology and immunology, 2(3), 139–141. https://doi.org/10.1111/j.1399-302x.1987.tb00279.xspa
dc.relation.referencesSlots, J., Bragd, L., Wikström, M., & Dahlén, G. (1986). The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults. Journal of clinical periodontology, 13(6), 570–577. https://doi.org/10.1111/j.1600-051x.1986.tb00849.xspa
dc.relation.referencesSmalley, J. W., & Olczak, T. (2017). Heme acquisition mechanisms of Porphyromonas gingivalis strategies used in a polymicrobial community in a heme-limited host environment. Molecular oral microbiology, 32(1), 1–23. https://doi.org/10.1111/omi.12149spa
dc.relation.referencesSmolen, J. S., Aletaha, D., & McInnes, I. B. (2016). Rheumatoid arthritis. Lancet (London, England), 388(10055), 2023–2038. https://doi.org/10.1016/S0140-6736(16)30173-8spa
dc.relation.referencesStobernack, T., Glasner, C., Junker, S., Gabarrini, G., de Smit, M., de Jong, A., Otto, A., Becher, D., van Winkelhoff, A. J., & van Dijl, J. M. (2016). Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis. Journal of proteome research, 15(12), 4532–4543. https://doi.org/10.1021/acs.jproteome.6b00634spa
dc.relation.referencesStructural Genomics Consortium, China Structural Genomics Consortium, Northeast Structural Genomics Consortium, Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., Knapp, S., Oppermann, U., Arrowsmith, C., Hui, R., Ming, J., dhe-Paganon, S., Park, H. W., Savchenko, A., Yee, A., Edwards, A., Vincentelli, R., … Gunsalus, K. C. (2008). Protein production and purification. Nature methods, 5(2), 135–146. https://doi.org/10.1038/nmeth.f.202spa
dc.relation.referencesSun, J., Zheng, Y., Bian, X., Ge, H., Wang, J., & Zhang, Z. (2021). Non-surgical periodontal treatment improves rheumatoid arthritis disease activity: a meta-analysis. Clinical oral investigations, 25(8), 4975–4985. https://doi.org/10.1007/s00784-021-03807-wspa
dc.relation.referencesThermofisher. (2020). One Shot™ TOP10 Chemically Competent E. coli. [online] Available at: https://www.thermofisher.com/order/catalog/product/C404003?SID=srch-srp-C404003#/C404003?SID=srch-srp-C404003 [Accessed 7 Mar. 2020].spa
dc.relation.referencesTheilade, E., Wright, W. H., Jensen, S. B., & Löe, H. (1966). Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation. Journal of periodontal research, 1, 1–13. https://doi.org/10.1111/j.1600-0765.1966.tb01842.xspa
dc.relation.referencesUnriza-Puin, S., Bautista-Molano, W., Lafaurie, G. I., Valle-Oñate, R., Chalem, P., Chila-Moreno, L., Bello-Gualtero, J. M., & Romero-Sánchez, C. (2017). Are obesity, ACPAs and periodontitis conditions that influence the risk of developing rheumatoid arthritis in first-degree relatives?. Clinical rheumatology, 36(4), 799–806. https://doi.org/10.1007/s10067-016-3519-zspa
dc.relation.referencesUhlig, T., Hagen, K. B., & Kvien, T. K. (1999). Current tobacco smoking, formal education, and the risk of rheumatoid arthritis. The Journal of rheumatology, 26(1), 47–54.spa
dc.relation.referencesUsui, M., Onizuka, S., Sato, T., Kokabu, S., Ariyoshi, W., & Nakashima, K. (2021). Mechanism of alveolar bone destruction in periodontitis - Periodontal bacteria and inflammation. The Japanese dental science review, 57, 201–208. https://doi.org/10.1016/j.jdsr.2021.09.005spa
dc.relation.referencesVeillard, F., Potempa, B., Guo, Y., Ksiazek, M., Sztukowska, M. N., Houston, J. A., Koneru, L., Nguyen, K. A., & Potempa, J. (2015). Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphyromonas gingivalis. Biological chemistry, 396(4), 377–384. https://doi.org/10.1515/hsz-2014-0304spa
dc.relation.referencesVermilyea, D. M., Moradali, M. F., Kim, H. M., & Davey, M. E. (2021). PPAD Activity Promotes Outer Membrane Vesicle Biogenesis and Surface Translocation by Porphyromonas gingivalis. Journal of bacteriology, 203(4), e00343-20. https://doi.org/10.1128/JB.00343-20spa
dc.relation.referencesVermilyea, D. M., Ottenberg, G. K., & Davey, M. E. (2019). Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381. NPJ biofilms and microbiomes, 5(1), 7. https://doi.org/10.1038/s41522-019-0081-xspa
dc.relation.referencesVidal, J., Canizález-Román, A., Gutiérrez-Jiménez, J., Navarro-García, F. (2007) Patogénesis molecular, epidemiología y diagnóstico de Escherichia coli enteropatógena. Salud Pública de México. 2007;49(5), 376-386.spa
dc.relation.referencesVossenaar, E. R., Radstake, T. R., van der Heijden, A., van Mansum, M. A., Dieteren, C., de Rooij, D. J., Barrera, P., Zendman, A. J., & van Venrooij, W. J. (2004). Expression and activity of citrullinating peptidylarginine deiminase enzymes in monocytes and macrophages. Annals of the rheumatic diseases, 63(4), 373–381. https://doi.org/10.1136/ard.2003.012211spa
dc.relation.referencesWegner, N., Wait, R., Sroka, A., Eick, S., Nguyen, K. A., Lundberg, K., Kinloch, A., Culshaw, S., Potempa, J., & Venables, P. J. (2010). Peptidylarginine deiminase from Porphyromonas gingivalis citrullinates human fibrinogen and α-enolase: implications for autoimmunity in rheumatoid arthritis. Arthritis and rheumatism, 62(9), 2662–2672. https://doi.org/10.1002/art.27552spa
dc.relation.referencesWilensky, A., Polak, D., Houri-Haddad, Y., & Shapira, L. (2013). The role of RgpA in the pathogenicity of Porphyromonas gingivalis in the murine periodontitis model. Journal of Clinical Periodontology, 40(10), 924–932. https://doi.org/10.1111/jcpe.12139spa
dc.relation.referencesWingrove, J. A., DiScipio, R. G., Chen, Z., Potempa, J., Travis, J., & Hugli, T. E. (1992). Activation of complement components C3 and C5 by a cysteine proteinase (gingipain-1) from Porphyromonas (Bacteroides) gingivalis. The Journal of biological chemistry, 267(26), 18902–18907.spa
dc.relation.referencesWitalison, E. E., Thompson, P. R., & Hofseth, L. J. (2015). Protein Arginine Deiminases and Associated Citrullination: Physiological Functions and Diseases Associated with Dysregulation. Current drug targets, 16(7), 700–710. https://doi.org/10.2174/1389450116666150202160954spa
dc.relation.referencesWirth, R., Pap, B., Maróti, G., Vályi, P., Komlósi, L., Barta, N., Strang, O., Minárovits, J., & Kovács, K. L. (2021). Toward Personalized Oral Diagnosis: Distinct Microbiome Clusters in Periodontitis Biofilms. Frontiers in cellular and infection microbiology, 11, 747814. https://doi.org/10.3389/fcimb.2021.747814spa
dc.relation.referencesXiao, L., Zhang, Q., Peng, Y., Wang, D., & Liu, Y. (2020). The effect of periodontal bacteria infection on incidence and prognosis of cancer: A systematic review and meta-analysis. Medicine, 99(15), e19698. https://doi.org/10.1097/MD.0000000000019698spa
dc.relation.referencesXie H. (2015). Biogenesis and function of Porphyromonas gingivalis outer membrane vesicles. Future microbiology, 10(9), 1517–1527. https://doi.org/10.2217/fmb.15.63spa
dc.relation.referencesXu, H., Zhao, H., Fan, D., Liu, M., Cao, J., Xia, Y., Ju, D., Xiao, C., & Guan, Q. (2020). Interactions between Gut Microbiota and Immunomodulatory Cells in Rheumatoid Arthritis. Mediators of inflammation, 2020, 1430605. https://doi.org/10.1155/2020/1430605spa
dc.relation.referencesYamakawa, M., Ouhara, K., Kajiya, M., Munenaga, S., Kittaka, M., Yamasaki, S., Takeda, K., Takeshita, K., Mizuno, N., Fujita, T., Sugiyama, E., & Kurihara, H. (2016). Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice. Clinical and experimental immunology, 186(2), 177–189. https://doi.org/10.1111/cei.12847spa
dc.relation.referencesYuzhalin A. E. (2019). Citrullination in Cancer. Cancer research, 79(7), 1274–1284. https://doi.org/10.1158/0008-5472.CAN-18-2797spa
dc.relation.referencesZhou, N., Zou, F., Cheng, X., Huang, Y., Zou, H., Niu, Q., Qiu, Y., Shan, F., Luo, A., Teng, W., & Sun, J. (2021). Porphyromonas gingivalis induces periodontitis, causes immune imbalance, and promotes rheumatoid arthritis. Journal of leukocyte biology, 110(3), 461–473. https://doi.org/10.1002/JLB.3MA0121-045Rspa
dc.relation.referencesZhou, X. Y., Gao, J. L., Hunter, N., Potempa, J., & Nguyen, K. A. (2013). Sequence-independent processing site of the C-terminal domain (CTD) influences maturation of the RgpB protease from Porphyromonas gingivalis. Molecular microbiology, 89(5), 903–917. https://doi.org/10.1111/mmi.12319spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc610 - Medicina y salud::616 - Enfermedadesspa
dc.subject.otherCromatografíaspa
dc.subject.otherArtritis Reumatoidespa
dc.subject.otherArthritis, Rheumatoideng
dc.subject.otherChromatographyeng
dc.subject.proposalPorphyromonas gingivalis
dc.subject.proposalGingipaínasspa
dc.subject.proposalRgpA
dc.subject.proposalPPAD
dc.subject.proposalArtritis Reumatoideaspa
dc.subject.proposalPeriodontitisspa
dc.subject.proposalGingipainseng
dc.subject.proposalRheumatoid arthritiseng
dc.titlePresencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoidespa
dc.title.translatedPresence of RgpA, anti-RgpA antibodies and their association with rheumatoid arthritiseng
dc.typeTrabajo de grado - Doctoradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_db06spa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/doctoralThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TDspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.fundernameMinciencias- Ministerio de Ciencia Tecnología e Innovación.spa

Archivos

Bloque original

Mostrando 1 - 1 de 1
Miniatura
Nombre:
52882038.2022.pdf
Tamaño:
3.13 MB
Formato:
Adobe Portable Document Format
Descripción:
Tesis Doctorado en Biotecnología
Descargar

Bloque de licencias

Mostrando 1 - 1 de 1
Miniatura
Nombre:
license.txt
Tamaño:
5.74 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Doctorado en Biotecnología