Prevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotes

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dc.contributor.advisorMuñoz Flórez, Jaime Eduardo
dc.contributor.authorOsorio Rueda, Juan Gabriel
dc.contributor.educationalvalidatorMontoya Lerma, James
dc.contributor.researchgroupGrupo de Investigación en Diversidad Biológicaspa
dc.date.accessioned2022-08-25T21:09:36Z
dc.date.available2022-08-25T21:09:36Z
dc.date.issued2022-08-24
dc.descriptionIlustraciones, tablasspa
dc.description.abstractLas bacterias simbiontes habitan una gran parte de los artrópodos en el mundo, incluidas las hormigas (Hymenoptera: Formicidae). Algunos de estos endosimbiontes manipulan la reproducción, como los géneros Rickettsia y Wolbachia, destacándose en muchos aspectos de la vida del huésped. Aunque tienen un potencial biotecnológico en el manejo integrado de plagas, se desconoce el estatus de infección y prevalencia en el ciclo de vida de especies de alto impacto económico como la hormiga arriera Atta cephalotes. Se evaluaron un conjunto de cebadores específicos para Rickettsia, gen Rb, y Wolbachia, gen wsp, empleando una PCR anidada con la finalidad de detectar estos niveles de infección en los estadios larva, pupa y adulto de muestras colectadas en diferentes nidos de esta hormiga arriera en el Valle del Cauca. Encontramos para una banda de 900 pb que la infección por Rickettsia (27.7% de los nidos) es relativamente común en comparación con Wolbachia quien no fue detectada en este estudio. Se identificó una probable especie procariota endosimbionte Rickettsia sp. y a pesar del desconocimiento de los efectos fisiológicos de este microrganismo sobre la hormiga arriera, comprende uno de los primeros pasos en Colombia para profundizar en la biología y ecología de esta fascinante interacción. (Texto tomado de la fuente)spa
dc.description.abstractSymbiotic bacteria inhabit a large part of the world's arthropods, including ants (Hymenoptera: Formicidae). Some of these endosymbionts manipulate reproduction, such as the Rickettsia and Wolbachia genera, excelling in many aspects of host life. Although they have biotechnological potential in integrated pest management, the infection status and prevalence in the life cycle of species with a high economic impact, such as the leafcutter ant Atta cephalotes, are unknown. A set of specific primers for Rickettsia, Rb gene, and Wolbachia, wsp gene, were evaluated using a nested PCR to detect these levels of infection in the larval, pupal, and adult stages of samples collected in different nests of this leafcutter ant. in the Valle del Cauca. We found for a 900 bp band that Rickettsia infection (27.7% of nests) is relatively common compared to Wolbachia which was not detected in this study. A probable endosymbiotic prokaryotic species Rickettsia sp. and despite the lack of knowledge of the physiological effects of this microorganism on the ant, it comprises one of the first steps in Colombia to deepen the biology and ecology of this fascinating interaction.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Biológicasspa
dc.description.methodsSe evaluaron un conjunto de cebadores específicos para Rickettsia, gen Rb, y Wolbachia, gen wsp, empleando una PCR anidada con la finalidad de detectar estos niveles de infección en los estadios larva, pupa y adulto de muestras colectadas en diferentes nidos de esta hormiga arriera en el Valle del Cauca.spa
dc.format.extentxiv, 43 páginas + anexosspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/82123
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Palmiraspa
dc.publisher.facultyFacultad de Ciencias Agropecuariasspa
dc.publisher.placePalmira Valle del Cauca, Colombiaspa
dc.publisher.programPalmira - Ciencias Agropecuarias - Maestría en Ciencias Biológicasspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesspa
dc.subject.proposalEndosimbiontesspa
dc.subject.proposalWolbachiaspa
dc.subject.proposalRickettsiaspa
dc.subject.proposalHormigaspa
dc.subject.proposalInfecciónspa
dc.subject.proposalEndosymbiontseng
dc.subject.proposalWolbachiaeng
dc.subject.proposalRickettsiaeng
dc.subject.proposalAnteng
dc.subject.proposalInfectioneng
dc.titlePrevalencia y estatus de infección de los endosimbiontes Wolbachia spp. y Rickettsia spp. en poblaciones de Atta cephalotesspa
dc.title.translatedPrevalence and infection status of endosymbionts Wolbachia spp. and Rickettsia spp. in populations of Atta cephaloteseng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
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dc.type.driverinfo:eu-repo/semantics/masterThesisspa
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dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
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