Evaluación técnico-económica preliminar de la producción de Butanol mediante intensificación de fermentación ABE, utilizando Clostridium acetobutylicum IBUN IV, y destilación Flash

dc.contributor.advisorMartínez Riascos, Carlos Arturo
dc.contributor.advisorBernal Morales, Jose Mauricio
dc.contributor.authorMuñoz Castrillón, Nicolás
dc.contributor.researchgroupBioprocesos y Bioprospección
dc.date.accessioned2025-12-16T19:57:01Z
dc.date.available2025-12-16T19:57:01Z
dc.date.issued2025
dc.descriptionilustraciones a color, diagramas, fotografías, tablasspa
dc.description.abstractEste trabajo presenta una evaluación preliminar de la producción de butanol mediante intensificación de la fermentación ABE, utilizando la cepa Clostridium acetobutylicum IBUN IV, caracterizada por su alta tolerancia al butanol y por no producir etanol. Se desarrolló un modelo cinético específico basado en datos experimentales en biorreactor, que contempla la limitación de sustrato y la inhibición tanto por sustrato como por productos. Adicionalmente, se estudió el equilibrio líquido-vapor del caldo fermentado mediante un diseño experimental en un equipo con reflujo, a partir del cual se construyó un modelo de superficie de respuesta de segundo orden. Con esta base, se evaluaron configuraciones para el proceso, incluyendo la propuesta que combina fermentación cíclica con biorreactores en serie y una etapa inicial de separación por destilación flash, simulando la fermentación en MATLAB® y la secuencia de separación en Aspen Plus®. Posteriormente, se estimaron los costos fijos y operativos de una planta con capacidad de 3.000 toneladas anuales de butanol, utilizando el software APEA® y ecuaciones de dimensionamiento de equipos. Aunque la integración tecnológica mostró beneficios en la reducción de los requerimientos energéticos, la evaluación financiera arrojó un Valor Presente Neto (VPN) negativo, una Tasa Interna de Retorno (TIR) de 0,08 % y un periodo de recuperación de 14,9 años, lo cual indica que el proceso no es económicamente viable bajo las condiciones evaluadas. No obstante, el análisis para una cepa con mayor producción de butanol, muestra que este cambio permitiría mejorar los indicadores económicos, aunque sin alcanzar aún la viabilidad financiera del proyecto (Texto tomado de la fuente).spa
dc.description.abstractThis work presents a preliminary techno-economic evaluation of butanol production through the intensification of ABE fermentation, using the strain Clostridium acetobutylicum IBUN IV, characterized by its high tolerance to butanol and its inability to produce ethanol. A specific kinetic model was developed based on experimental data obtained in a bioreactor, accounting for substrate limitation as well as inhibition by both substrate and product. Additionally, the vapor-liquid equilibrium of the fermented broth was studied through an experimental design implemented in a reflux-based system, from which a second-order response surface was constructed. On this basis, an integrated process was designed, combining fermentation with an initial separation stage via flash distillation. Fermentation was simulated in MATLAB®, and the separation sequence was modeled in Aspen Plus®. Subsequently, fixed and operating costs were estimated for a plant with a capacity of 3,000 tons of butanol per year, using APEA® software and equipment sizing equations. Although the proposed integration showed technical benefits, particularly in reducing energy requirements, the financial evaluation resulted in a negative Net Present Value (NPV), an Internal Rate of Return (IRR) of 0.08%, and a payback period of 14.9 years, indicating that the process is not economically viable under the evaluated conditions. Nevertheless, an alternative strain with higher butanol production was proposed, leading to improved economic indicators, although still falling short of achieving overall financial viability for the project.eng
dc.description.degreelevelMaestría
dc.description.degreenameMagíster en Ingeniería Química
dc.description.methodsSe utilizó la cepa Clostridium acetobutylicum IBUN IV del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN), la cual fue obtenida por mutación a partir de la cepa de referencia Clostridium acetobutylicum DSM 1732, y fue seleccionada en un estudio previo del propio grupo de investigación, presentado en Tapias et al. (2024), donde se comparó con la cepa ATCC 824 y con la cepa IBUN 125C. Así mismo, la selección del medio de cultivo se presenta en el mismo trabajo, en el cual se evaluó la cepa IBUN IV en los medios RCM y T6, resultando en mejor producción de butanol este último.
dc.description.researchareaBioprocesos
dc.format.extentxvi, 83 páginas
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/89215
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
dc.publisher.facultyFacultad de Ingeniería
dc.publisher.placeBogotá, Colombia
dc.publisher.programBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería Química
dc.relation.referencesAiba, S., Shoda, M., & Nagatani, M. (1968). Kinetics of Product Inhibition in Alcohol Fermentation. Biotechnology and Bioengineering, 845-864.
dc.relation.referencesAlavijeh, M., & Karimi, K. (2019). Biobutanol production from corn stover in the US. Industrial Cops & Products, 641-653.
dc.relation.referencesAspen Technology, Inc. (2001). Aspen Plus 11.1 User Guide (Versión 11.1). Aspen Technology, Inc.
dc.relation.referencesAspen Technology, Inc. (2019). Aspen Capital Cost Estimator: User Guide (Version 11). AspenTech.
dc.relation.referencesBaral, N. R., & Shah, A. (2014). Microbial inhibitors: formation and effects on acetone-butanol-etanol fermentation of lignocellulosic biomass. Applied Microbiology and Biotechnology, 9151-9172.
dc.relation.referencesBiebl, H. (1999). Clostridium acetobutylicum. Academic Press, 445-451.
dc.relation.referencesBureau of Energy Efficiency. (2015). Cooling Towers. En Energy efficiency in electrical utilities: Guide book for national certification examination for energy managers and energy auditors. Government of India, Ministry of Power.
dc.relation.referencesBurhani, D., Triwahyuni, E., & Setiawan, R. (2019). Second-Generation Biobutanol: An Update. Reaktor, 101-110.
dc.relation.referencesCastellanos Suárez, L. J., Matallana Pérez, L. G., & López Giraldo, L. J. (2014). Análisis de estabilidad de un sistema de fermentación acetona-butanol-etanol (ABE) a partir de glucosa empleando Clostridium acetobutylicum ATCC 824. Revista Digital de la Facultad de Ciencias Naturales e Ingeniería de la UJTL, 15-23.
dc.relation.referencesChasoy Rojas, W. A. (2012). Determinación experimental del equilibrio líquido-vapor del sistema etanol-agua-glicerina. Bogotá: Universidad Nacional de Colombia.
dc.relation.referencesDiallo, M., Kengen, S., & Lopez, A. (2021). Sporulation in solventogenic and acetogenic clostridia. Applied Microbiology and Biotechnology, 3533-3557.
dc.relation.referencesDolphin Centrifuge. (s.f.). Alfa Laval centrifuge selection guide. Recuperado el 01 de 07 de 2025, de https://dolphincentrifuge.com/alfa-laval-centrifuge-selection-guide/
dc.relation.referencesDolphin Centrifuge. (s.f.). Disc Stack Centrifuge | Benefits, Costs, Operation, Specs, Sizing. Recuperado el 01 de 07 de 2025, de https://dolphincentrifuge.com/disc-stack-centrifuge/
dc.relation.referencesDoran, P. M. (2013). Bioprocess Engineering Principles. Oxford: Elsevier.
dc.relation.referencesEzeji, T. C., Qureshi, N., & Blaschek, H. P. (2004). Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microbiol Biotechnol, 653-658.
dc.relation.referencesGalinha, C. F., Sanches, S., & Crespo, J. G. (2019). Membrane bioreactors. En Fundamental Modeling of Membrane Systems (págs. 209-249). Elsevier.
dc.relation.referencesGreenhouse Gas Technology Center. (2012). Environmental Technology Verification Report: Climate Energy freewatt™ Micro-Combined Heat and Power System. Southern Research Institute; U.S. Environmental Protection Agency.
dc.relation.referencesHan, K., & Levenspiel, O. (1988). Extended Monod Kinetics for Substrate, Product, and Cell Inhibition. Biotechnology and Bioengineering, 430-437.
dc.relation.referencesHenao, L. V. (2014). Modelo matemático y simulación para mejorar la producción de butanol en la fermentación ABE. Universidad de Valladolid.
dc.relation.referencesHönig, V., Smrčka, L., Küüt, A., & Ilves, R. (2015). Adding biobutanol to diesel fuel and impact on fuel blend parameters. Estonian University of Life Sciences, 13(5), 1227-1233.
dc.relation.referencesHsieh, C., Hamid, U., Wu, H., Valentino, L., Urgun, M., & Chen, C. (2025). Thermodynamic modeling of aqueous acetic acid, butyric acid, and lactic acid solutions. Fluid Phase Equilibria.
dc.relation.referencesHuidong, C., Di, C., Changjing, C., Changwei, Z., Jianhong, W., & Peiyong, Q. (2019). Techno-economic analysis of acetone-butanol-ethanol distillation sequences feeding the biphasic condensate after in situ gas stripping separation. Separation and Purification Technology, 241-248.
dc.relation.referencesJang, Y.-S., Han, M.-J., Lee, J., Im, J. A., Lee, Y. H., Papoutsakis, E. T., . . . Lee, S. Y. (2014). Proteomic analyses of the phase transition from acidogenesis to solventogenesis using solventogenic and non-solventogenic Clostridium acetobutylicum strains. Applied Microbiology and Biotechnology, 5105-5115.
dc.relation.referencesJiang, Y., Liu, J., Jiang, W., Yang, Y., & Yang, S. (2014). Current status and prospects of industrial bio-production of n-butanol in China. Biotechnology Advances.
dc.relation.referencesKhamaiseh, E., Hamid, A., Abdeshahian, P., Yusoff, W., & Kalil, M. (2014). Enhanced Butanol Production by Clostridium acetobutylicum NCIMB 13357 Grown on Date Fruit as Carbon Source in P2 Medium. The Scientific World Journal.
dc.relation.referencesKolstad, C. (2024). Stainless Steel 304 and 316. Tameson. Obtenido de Stainless Steel 304 and 316: https://tameson.com/pages/stainless-steel-304-and-316
dc.relation.referencesKushwaha, D., Srivastava, N., Mishra, I., Upadhyay, S., & Mishra, P. (2018). Recent trends in biobutanol production. Reviews in Chemical Engineering.
dc.relation.referencesŁącki, K., Joseph, J., & Eriksson, K. (2018). Downstream Process Design, Scale-Up Principles, and Process Modeling. En Biopharmaceutical Processing: Development, Design, and Implementation of Manufacturing Processes. Elsevier.
dc.relation.referencesLi, S.-B., Qian, Y., Liang, Z.-W., Guo, Y., Zhao, M.-M., & Pang, Z.-W. (2016). Enhanced butanol production from cassava with Clostridium acetobutylicum by genome shuffling. World Journal of Microbiology and Biotechnology.
dc.relation.referencesLiu, Y. (2007). Overview of some theoretical approaches for derivation of the Monod equation. Applied Microbiology and Biotechnology, 1241-1250.
dc.relation.referencesLodi, G., De Guido, G., & Pellegrini, L. (2017). Simulation and energy analysis of the ABE fermentation integrated with gas stripping. Chemical Engineering Transactions, 139-144.
dc.relation.referencesLu, K. M., Chiang, Y. S., Wang, Y. R., Chein, R. Y., & Li, S. Y. (2016). Perfomance of fed-batch acetone-butanol-ethanol (ABE) fermentation coupled with the integrated in situ extraction-gas stripping process and the fractional condensation. Journal of the Taiwan Institute of Chemical Engineers, 119-123.
dc.relation.referencesLuong, J. (1987). Generalization of Monod Kinetics for Analysis of Growth Data with Substrate Inhibition. Biotechnology and Bioengineering, 242-248.
dc.relation.referencesManisalidis, I., Stavropoulou, E., & Bezirtzoglou, E. (2019). Environmental and Health Impacts of Air Pollution: A review. Frontiers on Public Health.
dc.relation.referencesMaxwell, C. (2025). Cost Indices. Towering Skills. Obtenido de https://toweringskills.com/financial-analysis/cost-indices/
dc.relation.referencesMayank, R., Ranjan, A., & Moholkar, V. S. (2012). Mathematical models of ABE fermentation: review and analysis. Critical Reviews in Biotechnology, 1-29.
dc.relation.referencesMillat, T., & Winzer, K. (2017). Mathematical modelling of clostridial acetone-butanol-ethanol fermentation. Applied Microbiology and Biotechnology, 2251-2271.
dc.relation.referencesMontoya, D., Spitia, S., Silva, E., & Schwarz, W. H. (2000). Isolation of mesophilic solvent-producing clostridia from Colombian sources: physiological characterization, solvent production and polysaccharide hydrolysis. Journal of Biotechnology, 117-126.
dc.relation.referencesMoon, H. G., Jang, Y.-S., Cho, C., Lee, J., Binkley, R., & Lee, S. Y. (2016). One hundred years of clostridial butanol fermentation. FEMS Microbiology Letters.
dc.relation.referencesMoreno, J. A., & Cubillos, J. A. (2017). Biobutanol como combustible: una alternativa sustentable. Investigación Joven, 4(1), 45-50.
dc.relation.referencesMuloiwa, M., Nyende-Byakika, S., & Dinka, M. (2020). Comparison of unstructured kinetic bacterial growth models. South African Journal of Chemical Engineering, 141-150.
dc.relation.referencesMyers, R., Montgomery, D., & Cook, C. (2009). Response Surface Methodology: Process and Product Optimization Using Designed Experiments. New Jersey: John Wiley & Sons.
dc.relation.referencesNath, S. (2024). Biotechnology and biofuels: paving the way towards a sustainable and equitable energy for the future. Discover Energy.
dc.relation.referencesPapacek, S., Celikovsky, S., Stys, D., & Ruiz, J. (2007). BILINEAR SYSTEM AS A MODELLING FRAMEWORK FOR ANALYSIS OF MICROALGAL GROWTH. Kybernetika, 1-20.
dc.relation.referencesPerim, G. R. (2019). Investigation of Vapor-Liquid and Liquid-Liquid Equilibria by COSMO-RS. Master’s thesis, Vienna University of Technology.
dc.relation.referencesPeters, M., & Timmerhaus, K. (1991). Plant design and economics for chemical engineers. Singapore: McGraw-Hill.
dc.relation.referencesPielak, G., & Miklos, A. (2010). Crowding and function reunite. PNAS, 17457-17458.
dc.relation.referencesPinto Mariano, A., Borba Costa, C. B., Wolf Maciel, M. R., Maugeri Filho, F., Pires Atala, D. I., de Franceschi de Angelis, D., & Maciel Filho, R. (2010). Dynamics and Control Strategies for a Butanol Fermentation Process. Applied Biochemistry and Biotechnology, 2424-2448.
dc.relation.referencesPrabhu, S. (s.f.). Disc Stack Centrifuge FAQ. Dolphin Centrifuge. Recuperado el 01 de Julio de 2025, de https://dolphincentrifuge.com/disc-stack-centrifuge-faq/
dc.relation.referencesQureshi, N., & Blaschek, H. (2001). Recent advances in ABE fermentation: hyper-butanol producing Clostridium beijerinckii BA101. Journal of Industrial Microbiology & Biotechnology, 287-291.
dc.relation.referencesRao, R. (2015). Boiler control system. En Power plant instrumentation and control handbook. Elsevier.
dc.relation.referencesSeader, J. D., Henley, E. J., & Roper, D. K. (2011). Separation Process Principles: Chemical and Biochemical Operations (Tercera ed.). John Wiley & Sons.
dc.relation.referencesSierra, J., Sierra, R., Montoya, D., Buitrago, G., & Silva, E. (1996). Obtención de mutantes espontáneas de Clostridium acetobutylicum resistentes al butanol. Revista Colombiana de Ciencias Químico-Farmacéuticas, 25(1), 26-35.
dc.relation.referencesSoltis, S. (2017). Water Use and Cycles of Concentration (COC) in a cooling tower. University of Illinois Urbana-Champaign. Obtenido de https://icap.sustainability.illinois.edu/project-update/water-use-and-cycles-concentration-coc-cooling-tower
dc.relation.referencesSteelpro Group. (2024). What is the density of stainless steel? Obtenido de https://steelprogroup.com/stainless-steel/properties/density
dc.relation.referencesTapias C, P. A., Rubiano M, K. M., Barreiro P, J. C., Muñoz Castrillón, N., Bernal M, J. M., & M. Riascos, C. A. (2024). A two-phase model for ABE fermentation with a modified Clostridium acetobutylicum strain. Chemical Enginerring Transactions, 121-126.
dc.relation.referencesTLV. (s.f.). Steam Table Calculator (Pressure). TLV Co., Ltd. Obtenido de https://toolbox.tlv.com/global/TI/calculator/steam-table-pressure.html
dc.relation.referencesTowler, G., & Sinnott, R. (2008). Chemical Engineering Design. Principles, Practice and Economics of Plant and Process Design. Elsevier Inc.
dc.relation.referencesVotruba, J., Volesky, B., & Yerushaimi, L. (1985). Mathematical model of a batch acetone-butanol fermentation. Biotechnology and Bioengineering, 247-255.
dc.relation.referencesZhou, X., Xu, D., & Jiang, T. (2017). Simplifying multidimensional fermentation dataset analysis and visualization: One step closer to capturing high-quality mutant strains. Scientific Reports.
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseReconocimiento 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ddc660 - Ingeniería química::668 - Tecnología de otros productos orgánicos
dc.subject.lembFERMENTACIONspa
dc.subject.lembFermentationeng
dc.subject.lembDESTILACIONspa
dc.subject.lembDistillationeng
dc.subject.lembSEPARACION (TECNOLOGIA)spa
dc.subject.lembSeparation (technology)eng
dc.subject.lembCOMBUSTIBLES LIQUIDOSspa
dc.subject.lembLiquid fuelseng
dc.subject.lembALCOHOL COMBUSTIBLEspa
dc.subject.lembAlcohol as fueleng
dc.subject.proposalClostridium acetobutylicumspa
dc.subject.proposalFermentación ABEspa
dc.subject.proposalIntensificación de procesosspa
dc.subject.proposalEvaluación técnico-económicaspa
dc.subject.proposalClostridium acetobutylicumeng
dc.subject.proposalABE fermentationeng
dc.subject.proposalProcess intensificationeng
dc.subject.proposalTechno-economic evaluationeng
dc.titleEvaluación técnico-económica preliminar de la producción de Butanol mediante intensificación de fermentación ABE, utilizando Clostridium acetobutylicum IBUN IV, y destilación Flashspa
dc.title.translatedPreliminary techno-economic evaluation of Butanol production through intensified ABE fermentation using Clostridium acetobutylicum IBUN IV and flash distillationeng
dc.typeTrabajo de grado - Maestría
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dcterms.audience.professionaldevelopmentInvestigadores
dcterms.audience.professionaldevelopmentEstudiantes
dcterms.audience.professionaldevelopmentMaestros
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2
oaire.awardtitleProducción de solventes por procesos intensificados con fermentación y separación acopladas
oaire.fundernameUniversidad Nacional de Colombia

Archivos

Bloque original

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
DocumentoFinal1075321439 version 2.pdf
Tamaño:
2.57 MB
Formato:
Adobe Portable Document Format
Descripción:
Tesis de Maestría en Ingeniería Química

Bloque de licencias

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
license.txt
Tamaño:
5.74 KB
Formato:
Item-specific license agreed upon to submission
Descripción: