Efecto de dos procesos de transesterificación del aceite de sacha inchi, sobre el método de cuantificación de los esteres metílicos mediante GC-FID
Cargando...
Archivos
Autores
Olivo-Taborda, Sara Juliana
Tipo de contenido
Document language:
Español
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Documentos PDF
Resumen
En esta investigación, se comparó la eficiencia en la conversión de los ácidos grasos, presentes en el aceite de sacha inchi, a metil ésteres aplicando dos métodos de transesterificación y posterior cuantificación por cromatografía de gases con detector FID (GC-FID); se aplicó un método de referencia descrito en la Norma Técnica Colombina NTC-4967 y otro enzimático, mediante el uso de una lipasa comercial de Candida rugosa, que ha sido estudiada para la producción de biodiesel a partir de ácidos grasos libres. Con la validación estadística de los métodos se establecieron las ventajas del método enzimático, dentro de las que se destaca el tiempo de reacción de 1h para la transformación de Triacil glicéridos en ésteres metílicos de ácidos grasos (FAMEs), estimados a partir, de la comparación de la eficiencia de la conversión para los ácidos grasos C16:0, C18:0, C18:1, C18:2, C18:3 y C20:0. Adicionalmente, se evaluó la concentración de enzima y solvente para definir las condiciones óptimas que permitieron alcanzar la máxima velocidad de conversión, obteniendo unas relaciones metanol/aceite de 1:1 y enzima/aceite de 0,01:1, estos resultados se establecieron aplicando un diseño de superficie de respuesta procesado en el software STATGRAPHICS® Centuriun XVI, en el que se usaron como variables independientes las relaciones metanol/aceite, enzima/aceite y tiempo de reacción.
In this investigation, the efficiency in the conversion in methyl esters of the fatty acids present in sacha inchi oil was compared, applying two transesterification methods, for the subsequent quantification by gas chromatography with FID detector (GC-FID); A reference method described in Colombian Technical Standard NTC-4967 and another enzymatic method was applied, using a commercial lipase from Candida rugosa, which has been studied for the production of biodiesel from free fatty acids. With the statistical validation of the methods, the advantages of the enzymatic method were established, among which the reaction time of 1 h for the transformation into methyl esters of fatty acids (FAMEs), estimated from the comparison of the efficiency, stands out. of the conversion for the fatty acids C16: 0, C18: 0, C18: 1, C18: 2, C18: 3 and C20: 0. Additionally, the concentration of enzyme and solvent was evaluated to define the optimal conditions that allowed reaching the maximum conversion speed, obtaining methanol / oil ratios of 1: 1 and enzyme / oil of 0.01: 1, these results were established applying a response surface design processed in the STATGRAPHICS® Centuriun XVI software, in which the methanol / oil, enzyme / oil and reaction time ratios were used as independent variables.
In this investigation, the efficiency in the conversion in methyl esters of the fatty acids present in sacha inchi oil was compared, applying two transesterification methods, for the subsequent quantification by gas chromatography with FID detector (GC-FID); A reference method described in Colombian Technical Standard NTC-4967 and another enzymatic method was applied, using a commercial lipase from Candida rugosa, which has been studied for the production of biodiesel from free fatty acids. With the statistical validation of the methods, the advantages of the enzymatic method were established, among which the reaction time of 1 h for the transformation into methyl esters of fatty acids (FAMEs), estimated from the comparison of the efficiency, stands out. of the conversion for the fatty acids C16: 0, C18: 0, C18: 1, C18: 2, C18: 3 and C20: 0. Additionally, the concentration of enzyme and solvent was evaluated to define the optimal conditions that allowed reaching the maximum conversion speed, obtaining methanol / oil ratios of 1: 1 and enzyme / oil of 0.01: 1, these results were established applying a response surface design processed in the STATGRAPHICS® Centuriun XVI software, in which the methanol / oil, enzyme / oil and reaction time ratios were used as independent variables.