Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares
dc.contributor.advisor | Blandón, Lina | |
dc.contributor.advisor | Campos Campos, Néstor Hernando | |
dc.contributor.advisor | Patiño, Albert | |
dc.contributor.author | Puentes, Erika Andrea | |
dc.contributor.hostingInstitution | Invemar - Instituto de Investigaciones marinas y costeras | |
dc.contributor.researchgroup | Fauna Marina Colombiana: Biodiversidad y Usos | spa |
dc.date.accessioned | 2024-07-17T21:55:09Z | |
dc.date.available | 2024-07-17T21:55:09Z | |
dc.date.issued | 2024-07-16 | |
dc.description | graficas, tablas | spa |
dc.description.abstract | La contaminación marina es una gran amenaza para la conservación de los ecosistemas y de su biodiversidad, por lo que se hace necesario realizar estudios enfocados en su recuperación, aplicando tecnologías ambientalmente amigables y de bajo costo como la biorremediación. La biorremediación es el uso de organismos, sus células, partes o metabolitos, para recuperar un ambiente contaminado a través de la transformación o degradación de compuestos tóxicos recalcitrantes como los hidrocarburos, en una forma menos tóxica o no tóxica. En este sentido, el uso de microalgas y/o sus componentes, es una alternativa prometedora ya que presentan tolerancia a factores de estrés como los ocasionados por la presencia de petróleo en el entorno, evidenciando la producción de metabolitos como biosurfactantes participantes de la actividad biorremediadora. Este proyecto tuvo como objetivo principal evaluar la respuesta metabólica a la presencia del petróleo, a través de la detección de biosurfactantes extracelulares de microalgas marinas aisladas del Mar Caribe colombiano, en condiciones de luz y oscuridad, empleando herramientas metabolómicas. Para ello, se realizó un screening de tolerancia celular con petróleo en suspensión al 1 %. Se evidencio la producción de un extracto extracelular rico en biosurfactantes, por Nannochloris sp., la cual al ser incubada en oscuridad produjo un extracto con mayor presencia de estos compuestos. Los resultados obtenidos, aportan información científica que contribuye a mejorar el conocimiento del proceso de tolerancia al petróleo que exhiben algunas microalgas marinas (Texto tomado de la fuente). | spa |
dc.description.abstract | Marine pollution is a significant threat to ecosystem conservation and its biodiversity. Therefore, it is necessary to carry out studies focused on ecosystem recovery, applying environmentally friendly and low-cost technologies such as bioremediation. Bioremediation is the use of organisms, their cells, parts or metabolites, to remediate a contaminated environment through the transformation or degradation of recalcitrant toxic compounds such as crude oil into a less toxic or non-toxic form. In this sense, using microalgae and /or its components is a promising alternative. Microalgae can be tolerant to stress factors such as oil crude, in response, they can produce metabolites like biosurfactants that participates in the bioremediation activity. The main objective of this project was to evaluate the metabolic response of microalgae isolated from the Colombian Caribbean, exposed to the presence of crude oil, as well as the detection of extracellular biosurfactants, under light and dark conditions, using metabolomic tools. A screening with crude oil in suspension at 1% was carried out, achieving evidence of the production of extracellular extract rich in biosurfactants by a strain Nannochloris sp. The same strain cultured without light, produced this extract in greater quantity. The results provide scientific information that improves the knowledge of the tolerance process to crude oil that some marine microalgae exhibit. | eng |
dc.description.curriculararea | Otra. Sede Caribe | spa |
dc.description.degreelevel | Maestría | spa |
dc.description.degreename | Magíster en Ciencias - Biología | spa |
dc.description.methods | Comparación de la producción de metabolitos extracelulares entre tratamientos: 1. Tratamiento de exposición a pétroleo: Incubados en luz y oscuridad 2. Grupos control, control abiotico de medio de cultivo con petróleo y control de crecimiento de las microalgas en medio de cultivo | spa |
dc.description.researcharea | Bioprospección marina | spa |
dc.format.extent | 100 paginas | spa |
dc.format.mimetype | application/pdf | spa |
dc.identifier.instname | Universidad Nacional de Colombia | spa |
dc.identifier.reponame | Repositorio Institucional Universidad Nacional de Colombia | spa |
dc.identifier.repourl | https://repositorio.unal.edu.co/ | spa |
dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/86548 | |
dc.language.iso | spa | spa |
dc.publisher | Universidad Nacional sede Caribe | spa |
dc.publisher.branch | Universidad Nacional de Colombia - Sede Caribe | spa |
dc.publisher.department | Centro de estudios en Ciencias del mar-CECIMAR | spa |
dc.publisher.faculty | Facultad de Ciencias | spa |
dc.publisher.place | San Andrés Islas | spa |
dc.publisher.program | Caribe - Caribe - Maestría en Ciencias - Biología | spa |
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dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.license | Atribución-NoComercial 4.0 Internacional | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | spa |
dc.subject.ddc | 500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados | spa |
dc.subject.ddc | 570 - Biología::579 - Historia natural microorganismos, hongos, algas | spa |
dc.subject.ddc | 600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionados | spa |
dc.subject.proposal | Contaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia, | |
dc.subject.proposal | Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolites | |
dc.title | Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares | spa |
dc.title.translated | Effect of crude oil on marine microalgae, about heterotrophic growth and the production of extracellular metabolites n of extracellular metabolites | eng |
dc.type | Trabajo de grado - Maestría | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
dc.type.redcol | http://purl.org/redcol/resource_type/TM | spa |
dc.type.version | info:eu-repo/semantics/acceptedVersion | spa |
dcterms.audience.professionaldevelopment | Estudiantes | spa |
oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
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