Extracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia.
| dc.contributor.advisor | Espinosa Hernández, Armando | spa |
| dc.contributor.author | Ramos Farfán, Andrés Felipe | spa |
| dc.contributor.researchgroup | Bioprocesos y bioprospección | spa |
| dc.date.accessioned | 2020-03-10T14:01:47Z | spa |
| dc.date.available | 2020-03-10T14:01:47Z | spa |
| dc.date.issued | 2019-07-19 | spa |
| dc.description.abstract | Este trabajo tiene como objetivo estudiar la extracción, purificación y modificación del biopolímero poli(3-hidroxibutirato) producido mediante la fermentación de ácidos grasos con la cepa mutante Burkholderia cepacia B27 con el fin de obtener un proceso novedoso y ambientalmente sostenible para un eventual escalamiento del proceso, y un material con propiedades mecánicas y térmicas que le permitan ser procesado con mayor facilidad y así obtener más salidas en el mercado de los materiales poliméricos. En el trabajo se propone un método novedoso y eficiente para la extracción del polímero usando ácido acético en vez de cloroformo, siendo la nueva metodología más segura para quien la desarrolle debido a la naturaleza de los solventes usados, y ambientalmente más sostenible debido a la significativa reducción de solventes halogenados en el proceso. Por otro lado, se modificó el polímero purificado usando diferentes aditivos, para evaluar el efecto de estos sobre las propiedades mecánicas del material final, y se determinó que las mezclas P3HB/PEG/PLA en diferentes rangos de concentraciones, presentan propiedades mecánicas significativamente mejores a las del P3HB puro que permiten ampliar su espectro de aplicaciones. Este trabajo fue desarrollado con el patrocinio de Colciencias, Biopolab y el Instituto de Biotecnología de la Universidad Nacional, y se desarrolló en el laboratorio de Fermentaciones del IBUN bajo la dirección de Armando Espinosa, Iván Cabeza y Nubia Moreno. | spa |
| dc.description.abstract | The aim of this work is to study the extraction, purification and modification of the poly(3-hydroxybutyrate) biopolymer produced by the fermentation of fatty acids with the mutant bacteria strain Burkholderia cepacia B27, in order to obtain a novel and environmentally sustainable process for an eventual scaling up, and a material with mechanical and thermal properties that allow it to be processed more easily and thus obtain more oportunities in the market for polymeric materials. The work proposes a new and efficient method for the extraction of the polymer using acetic acid instead of chloroform, being the new methodology safer for the developer due to the nature of the solvents used, and environmentally more sustainable due to the significant reduction of halogenated solvents in the process. On the other hand, the purified polymer was modified using different additives, to evaluate the effect of these on the mechanical properties of the final material, and it was determined that the P3HB/PEG/PLA mixtures in different concentration ranges present significantly better mechanical properties than pure P3HB, which allows to extend its spectrum of applications. This work was developed with the sponsorship of Colciencias, Biopolab and the Institute of Biotechnology of the National University, and was carried out in the Fermentations laboratory of IBUN under the direction of Armando Espinosa, Iván Cabeza and Nubia Moreno. | spa |
| dc.description.additional | Magíster en Ingeniería Química. Línea de Investigación: Biopolímeros - Bioprocesos | spa |
| dc.description.degreelevel | Maestría | spa |
| dc.description.project | Portafolio 100 | spa |
| dc.description.sponsorship | Colciencias, Biopolab LTDA, IBUN | spa |
| dc.format.extent | 190 | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.citation | A.F. Ramos «Extracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia.» Tesis de Maestría en Ingeniería Química, 2020. | spa |
| dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/76043 | |
| dc.language.iso | spa | spa |
| dc.publisher.branch | Universidad Nacional de Colombia - Sede Bogotá | spa |
| dc.relation.references | [1] V. Siracusa, P. Rocculi, S. Romani y M. Dalla Rosa, «Biodegradable polymers for food packaging: A review",» Food Science & Technology vol 19, pp. 634 - 643, 2008. | spa |
| dc.relation.references | [2] Superintendencia de Servicios Públicos Domiciliarios, Presente y Futuro de la Disposición Final de Residuos en Colombia, Bogotá, Colombia, 2013. | spa |
| dc.relation.references | [3] E. Avendaño y B. Albarracín, Panorama actual de la situación mundial, nacional y distrital de los residuos sólidos, Bogotá: Universidad Santo Tomás, 2015. | spa |
| dc.relation.references | [4] A. Anjum, M. Zuber, K. Mahmood, A. Noreen, M. Naveed y S. Tabsum, «Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements,» International Journal of Biological Macromolecules, vol. 89, pp. 161 - 174 , 2016. | spa |
| dc.relation.references | [5] S. Ahmad, A. Hassan y M. Uzir, «Materials for food packaging applications based on bio-based polymer nancomposites: A review,» Journal of Thermoplastic Composite Materials, pp. 1 - 32, 2015. | spa |
| dc.relation.references | [6] M. Koller, H. Niebelschütz y G. Braunegg, «Strategies for recovery and purification of poly[(R)-3-hydroxyalkanoates] (PHA) biopolyesters from surronding biomass.,» Engineering in Life Sciences 13, pp. 549 - 562, 2013. | spa |
| dc.relation.references | [7] D. A. Mendez, I. O. Cabeza, N. C. Moreno y C. A. Martinez, «Mathematical Modelling and Scale-up of Batch Fermentation with Burkholderia cepacia B27 Using Vegetal Oil as Carbon Source to Produce Polyhydroxyalkanoates,» Chemical Engineering Transactions, vol. 46, pp. 277 - 282, 2016. | spa |
| dc.relation.references | [8] S. Syairah, M. Nurhezreen y K. Sudesh, «"Enhanced Recovery and Purification of P(3HB-co-HHx) from recombinant Cupriavidus necator using alkaline digestion method,» Biochemistry and Biotechnology, pp. 524 - 535, 2012. | spa |
| dc.relation.references | [9] S. Philip, T. Keshavarz y I. Roy, «Polyhydroxyalkanoates: biodegradable polymers with a range of applications,» Journal of Chemical Technology and Biotechnology, vol. 82, pp. 233 - 247, 2007. | spa |
| dc.relation.references | [10] R. M. Macrae y J. F. Wilkinson, «Poly-beta-hydroxybutyrate metabolism in washed suspensions of Bacillus cerus and Bacillus megaterium,» Journal of Genetic and Microbiology, vol. 19, pp. 210 - 222, 1958. | spa |
| dc.relation.references | [11] E. R. Howells, «Opportunities in biotechnology for the chemical industry,» Chemical Industry, vol. 8, pp. 508 - 511, 1982. | spa |
| dc.relation.references | [12] P. Greco y E. Martuscelli, «Crystallization behaviour of poly (ethylene oxide) from poly (3-hydroxybutyrate)/poly (ethylene oxide) blends: phase structuring, morphology and thermal behaviour.,» Polymer, vol. 32, nº 9, pp. 1647 - 1653, 1991. | spa |
| dc.relation.references | [13] M. Avella y E. Martuscelli, «Poly-D(3-hydroxybutyrate)/poly (ethylene oxide) blends: phase diagram, thermal and cristalization behaviour.,» Polymer, vol. 29, pp. 1731 - 1737, 1988. | spa |
| dc.relation.references | [14] K. Y. y Y. Doi, «Enzymatic degradation and morphologies of binary blends of microbial poly(3-hydroxybutyrate) with poly(e-caprolactone), poly(1,4-butylene adipate) and poly(vinyl acetate.,» Polymer Degradation and Stability Journal, vol. 36, pp. 241 - 248, 1992. | spa |
| dc.relation.references | [15] N. Lotti, M. Pizzoli, G. Ceccorulli y M. Scandola, «Binary blends of microbial poly(3-hydroxybutyrate) with polymethacrylates.,» Polymer, vol. 34, pp. 4935 - 4940, 1993. | spa |
| dc.relation.references | [16] N. Lotti y M. Scandola, «Miscibility of bacterial poly(3hydroxybutyrate-co-3-hydroxyvalerate) with ester substituted celluloses,» Polymer Bulletin, vol. 29, pp. 407 - 413, 1992. | spa |
| dc.relation.references | [17] N. Shogren, «Starch-poly(hydroxyalkanoate) composites and blends,» de Biodegradable polymer blends and composites from renewable resources, Hoboken, Wiley, 2009. | spa |
| dc.relation.references | [18] T. Paramasivan y A. Abdul Kamal, «On the study of indigenous natural-fibe composites,» Fibre Science and Technology, vol. 7, pp. 85 - 88, 1974. | spa |
| dc.relation.references | [19] A. Anderson y Dawers EA, «Ocurrence, metabolism, metabolic role and industrial use of bacterial polyhydroxyalkanoates.,» Microbiology Reviews, vol. 54, pp. 450 - 472, 1990. | spa |
| dc.relation.references | [20] K. Sudesh, Abe H. y Y. Doi, «Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters,» Progress In Polymer Science, vol. 25, pp. 1503 - 1555, 2000. | spa |
| dc.relation.references | [21] G. Barnard y J. Sanders, «The poly-beta-hydroxybutyrate granule in vivo. A new insight based on NMR spectroscopy of whole cells,» Journal of Biological Chemistry, vol. 6, pp. 3286 - 3291, 1989. | spa |
| dc.relation.references | [22] R. Gabriel, Z. Smith y J. Merrick, «Metabolism of poly-b-hydroxybutyrate. I. Purification, composition and properties of native poly-beta-hydroxybutyrate granules from Bacillus megaterium,» Biochemistry, vol. 7, pp. 3676 - 3681, 1968. | spa |
| dc.relation.references | [23] S. Kusaka, H. Abe, S. Lee y Y. Doi, «Molecular mass of poly[(R)-3-hydroxybutyric acid] produced in recombinant Escherichia coli.,» Applied Microbiology and Biotechnology Journal, vol. 47, pp. 140-143, 1997. | spa |
| dc.relation.references | [24] A. Anderson, D. Williams, B. Taidi, E. Dawes y D. Ewing, «Studies on copolyester synthesis by Rhodococus ruber and factors influencing the molecular mass of polyhydroxybutyrate accumulated by Methylobacterium extroquens and Alcaligenes eutrophus,» FEMS Microbiology Reviews, vol. 103, pp. 93 - 102, 1992. | spa |
| dc.relation.references | [25] P. Holmes, «Biologically produced (R)-3-hydroxyalkanoate polymers and copolymers,» de Developements in crystaline polymers Vol. 2, London, 1998, pp. 1 - 65. | spa |
| dc.relation.references | [26] Y. Doi, Microbial Polyesters, Nueva York: VCH Publishers, 1990. | spa |
| dc.relation.references | [27] Barham PJ y A. Keller, «Relationships between microstructure and mode of fracture in polyhydroxybutyrate,» Journal of Polymer Science, vol. 24, pp. 69 - 77, 1986. | spa |
| dc.relation.references | [28] G. De Koning y P. Lemstra , «Crystallization phenomena in bacterial poly[(R)-3-hydroxybutyrate]: 2, Embrittlement and Rejuvenation,» Polymer, vol. 34, pp. 4089 -4094, 1993. | spa |
| dc.relation.references | [29] Bugincourt E, P. Cinelli , Lazzeri A y Alvarez V, «Polyhydroxyalkanoates (PHAS): review of syhtnesis, characteristics, processing and potential applications in packaging,» Express Polymer Letters, vol. 8, pp. 791 - 808, 2014. | spa |
| dc.relation.references | [30] S. Angelini, C. Pierfrancesco, B. Immirzi, M. Poskovic, G. Santagata, G. Scarinzi y M. Malinconico, «From Microbial Biopolymers to Bioplastics: Suistainable Additives for PHB Processing and Stabilization,» de Microbial Factories, India, Springer, 2015, pp. 139 - 160. | spa |
| dc.relation.references | [31] T. Ojum, J. Yu y B. Solomon, «Production of Polyhydroxyalkanoates, a bacterial biodegradable polymer,» African Journal of Biotechnology, vol. 3, nº 1, pp. 18 - 24, 2004. | spa |
| dc.relation.references | [32] C. Loo, W. Lee, T. Tsuge, Y. Doi y K. Sudesh, «Biosynthesis and Characterization of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Palm Oil Products in a Wautersia eutropha Mutant,» Biotechnology Letters, vol. 27, nº 18, pp. 1405 - 1410, 2005. | spa |
| dc.relation.references | [33] K. Snell, V. Singh y S. Brumbley, «Production of nove biopolymers in plants: Recent technological advances and future prospects,» Current Opinion in Biotechnology, vol. 32, pp. 68 - 75, 2015. | spa |
| dc.relation.references | [34] R. Nonato, P. Mantelatto y C. Rosell, «Integrated production of biodegradable plastic, sugar and ethanol.,» Applied Microbiology and Biotechnology, vol. 57, pp. 1 - 5, 2001. | spa |
| dc.relation.references | [35] G. Jiang, D. Hill, M. Kowalczuk, B. Johnston, G. Adamus, V. Irorere y I. Radecka, «Carbon sources for polyhydroxyalkanoates and an integrated biorefinery,» International Journal of Molecular Sciences, vol. 17, 2016. | spa |
| dc.relation.references | [36] B. Laycock, P. Halley, S. Pratt, A. Werker y P. Lant, «The chemomechanical properties of microbial polyhydroxyalkanoates,» Progress in Polymer Science, vol. 39, pp. 397 - 442 , 2014. | spa |
| dc.relation.references | [37] M. Din, Z. Ujang, M. van Loosdrecht, A. Ahmad y M. Sairan, «Optimization of nitrogen and phosphorus limitation for better biodegradable plastic production and organic removal using single fed-batch mixed cultures and renewable resources.,» Water Science Technologies, vol. 53, pp. 15 - 20, 2006. | spa |
| dc.relation.references | [38] C. Du, J. Sabirova, W. Soetaert y S. Ki Carol Lin, «Polyhydroxyalkanoates Production From Low Cost Suistantable Raw Materials,» Current Chemical Biology, vol. 6, pp. 14 - 25, 2012. | spa |
| dc.relation.references | [39] J. Beun, K. Dircks, M. Van Loosdrecht y J. Heijnen, «Poly-beta-hydroxybutyrate metabolism in dynamically fed mixed microbial cultures». | spa |
| dc.relation.references | [40] S. Bengtsson, A. Pisco, M. Reis y P. Lemos, «Production of polyhydroxyalkanoates from fermented sugar cane molasses by mixed culture enriched in glycogen accumulating organism,» Journal of Biotechnology, vol. 145, nº 3, pp. 253 - 263, 2010. | spa |
| dc.relation.references | [41] S. Bengtsson, A. Werker, M. Christensson y T. Welander, «Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater,» Bioresource Technology, vol. 99, nº 3, pp. 509 - 516, 2008. | spa |
| dc.relation.references | [42] M. Md Din, Z. Ujang, M. Van Loosdrecht y M. Ahmad, «Polyhydroxyalkanoates (PHAs) Production from saponified sunflower oil in mixed cultures under aerobic condition,» Jurnal Teknologi, vol. 48, pp. 1 - 19, 2008. | spa |
| dc.relation.references | [43] H. Liu, P. Hall, J. Darby, E. Coats, P. Green, D. Thompson y F. Loge, «Production of Polyhydroxyalkanoates During Treatment of Tomato Cannery Wastewater,» Water Environment Research, vol. 80, pp. 367 - 372, 2008. | spa |
| dc.relation.references | [44] E. Coats, F. Loge, M. Wolcott, K. Englund y A. McDonald, «Synthesis of Polyhydroxyalkanoates in Municipal Wastewater Treatment,» Water Environment Research, vol. 79, nº 12, pp. 2396 - 2403, 2007. | spa |
| dc.relation.references | [45] D. Dionisi, M. Majone, C. Levantesi, A. Bellani y A. Fuoco, «Effect of feed length on settleability, substrate uptake and storage in a sequencing batch reactor treating an industrial wastewater,» Environmental Technology, vol. 27, pp. 901 - 908 , 2006. | spa |
| dc.relation.references | [46] D. Dionisi, G. Carucci, M. Petrangeli, C. Riccardi y F. Carrasco, «Olive oil mill effluents as a feedstock for production of biodegradable polymers,» Water Research, vol. 39, nº 10, pp. 2076 - 2084, 2005. | spa |
| dc.relation.references | [47] J. Ramsay, E. Berger, R. Voyer, C. Chavarie y B. Ramsay, «Extraction of poly-3-hydroxybutyrate using chlorinated solvents,» Biotechnology Techniques, vol. 8, pp. 589 - 594, 1994. | spa |
| dc.relation.references | [48] P. Barham y A. Selwood, «Extraction of poly-beta-hydroxybutyric acid». US Patente 4391766, 1983. | spa |
| dc.relation.references | [49] S. Harrison, H. Chase, S. Amor, K. Bonthrone y Sanders JK, «Plasticization of poly(hydroxybutyrate) in vivo,» International Journal of Biological Macromolecules, vol. 14, pp. 50 - 56, 1992. | spa |
| dc.relation.references | [50] S. Hu, A. McDonald y E. Coats, «Characterization of polyhydroxybutyrate biosynthesized from crude glycerol waste using mixed microbial consortia.,» Journal of Applied Polymer Science, vol. 129, pp. 1314 - 1321, 2013. | spa |
| dc.relation.references | [51] B. G., «Sistainable poly(hydroxyalkanoate) (PHA) production,» de Degradable Polymers: Principles and Applications - 2 Ed, Dordrecht, Scott G , 2003, pp. 235 - 293. | spa |
| dc.relation.references | [52] J. Xuan, J. Ramsay y B. Ramsay, «Acetone extraction of mcl_PHA from Pseudomonas putida KT2440,» Journal of Microbiology Methods, vol. 67, pp. 212 - 2199, 2006. | spa |
| dc.relation.references | [53] M. Koller, R. Bona, E. Chiellini y Braunegg G, «Extraction of short-chain-length poly[(R)-hydroxyalkanoates] (scl-PHA) by the "anti-solvent" acetone under elevated temperature and pressure,» Biotechnology Letters, 2013. | spa |
| dc.relation.references | [54] Walsem J, Van Zhong, S. Luhua y S. Simon , «Polymer extraction methods». US Patente Patent US7713720 B2, 2010. | spa |
| dc.relation.references | [55] A. Aramvash, N. Gholami-Banadkuki, F. Moazzeni-Zavareh y T. Hajizadeh, «An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents,» Journal of Microbiology Technology, vol. 25, nº 11, pp. 1936 - 1943, 2015. | spa |
| dc.relation.references | [56] Y. Yang, J. Jeon, D. Yi, J. Kim, H. Seo, C. Rha , A. Sinskey y C. Brigham, «Application of non-halogenated solvent, methyl ethyl ketone (MEK) for recovery of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(HB-co-HV)] from bacterial cells,» Biotechnology and Bioprocess Engineering , vol. 20, pp. 291 - 297, 2015. | spa |
| dc.relation.references | [57] A. Aramvash, F. Zavareh y N. Banadkuki, «Comparison of different solvents for extraction of polyhydroxybutyrate from Cupriavidus necator,» Engineering in Life Sciences, p. In Press, 2017. | spa |
| dc.relation.references | [58] H. Preusting, J. Kingma, G. Huisman y A. Steinbuchel, «Formation of polyester blends by a recombinant strain of Pseudomonas oleovorans: differen poly(3-hydroxyalkanoates) are stored in separate granules.,» Journal of Polymer Environmental, vol. 1, pp. 11 - 21, 1993. | spa |
| dc.relation.references | [59] R. Marchessault, F. Morin, S. Wong y I. Saracovan, «Artificial granule suspensions of long sidec chain poly (3-hydroxyalkanoate),» Canadian Journal of Microbiology, vol. 41, pp. 138 - 142, 1995. | spa |
| dc.relation.references | [60] W. Luzier, «Materials derived from biomass/biodegradable materials,» Proceedings of the National Academy of Science - USA, vol. 89, pp. 839 - 842, 1992. | spa |
| dc.relation.references | [61] M. Kim, K. Cho, H. Ryu y E. Lee, «Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulphate,» Biotechnology Letters, vol. 25, pp. 55 - 59, 2003. | spa |
| dc.relation.references | [62] J. Choi y S. Lee, «Efficient and economical recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals,» Biotechnology and Bioengineering, vol. 62, pp. 546 - 553, 2000. | spa |
| dc.relation.references | [63] W. Page y A. Cornish, «Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-beta-hydroxybutyrate,» Applied Environmentak Microbiology, vol. 53, pp. 4236 - 4244, 1993. | spa |
| dc.relation.references | [64] S. Hahn, Y. Chang, B. Kim y H. Chang, «Optimization of microbial poly(3-hydroxybutyrate) recovering using dispersions of sodium hypochlorite solution and chloroform,» Biotechnology and Bioengineering, vol. 44, pp. 256 - 261, 1994. | spa |
| dc.relation.references | [65] Merrick J.M y M. Doudoroff, «Depolimerization of poly-beta-hydroxybutyrate by an intracellurar enzyme system,» Journal of Bacteriology, vol. 88, pp. 60 - 71, 1964. | spa |
| dc.relation.references | [66] P. Holmes y G. Lim, «Separation Process». US Patente 4910145, 1990. | spa |
| dc.relation.references | [67] K. Yasotha, M. Aroua, Ramachandran K.B. y I. Tan, «Recovery of medium-chain-length polyhydroxyalkanoates (PHAs) through enzymatic digestion treatments and ultrafiltration.,» Biochemical Engineering Journal, vol. 30, pp. 260 - 268, 2006. | spa |
| dc.relation.references | [68] I. Tamer, M. Moo-Young y Y. Chisti, «Disruption of Alcaligenes latus for recovery of poly (b-hydroxybutyric acid): Comparision of high-pressure homogenization, bead milling and chemically induced lysis,» Industrial & Engineering Chemistry Research, vol. 37, pp. 1807 - 1814, 1998. | spa |
| dc.relation.references | [69] M. Ghatnekar, J. Pai y M. Ganesh, «Production and recovery of poly-3-hydroxybutyrate from Methylobacterium sp. V49,» Journal of Chemical Technology and Biotechnology, vol. 77, pp. 444 - 448, 2003. | spa |
| dc.relation.references | [70] K. Hwang, S. You y T. Don, «Disruption kinetics of bacterial cells during purification of poly-b-hydroxyalkanoates using ultrasonification,» Journal of Chinese Institute of Chemical Engineers, vol. 37, pp. 209 - 216, 2006. | spa |
| dc.relation.references | [71] C. Lu, Purification and separation of polyhydroxyalkanoates from bacteria., Tesis de Maestría - Yuan Ze University - Taiwan, 2006. | spa |
| dc.relation.references | [72] G. Breaunegg, G. Lefebvre y K. Genser, «Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects,» Journal of Biotechnology, vol. 65, pp. 127 - 161, 1998. | spa |
| dc.relation.references | [73] J. Teeka, T. Imai, A. Reungsang y X. Cheng, «Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolated Novosphingobium sp THA AIK7 using crude glycerol,» Journal of Industrial Microbiology and Biotechnology, pp. 1 - 10, 1999. | spa |
| dc.relation.references | [74] P. Hejazi, E. Vasheghani-Farahani y Y. Yamini, «Supercritical Fluid Disruption of Ralstonia eutropha for Poly(beta-hydroxybutyrate) Recovery,» Biotechnology Progress, vol. 19, pp. 1519 - 1523, 2008. | spa |
| dc.relation.references | [75] K. Khosravi-Darani, E. Vasheghani-Farahani, S. Shojaosadati y Y. Yamini, «Effect of Process Variables on Supercritical Fluid Disruption of Ralstonia eutropha cells for Poly(R-hydroxybutyrate) Recovery,» Biotechnology Progress, vol. 20, pp. 1757 - 1765, 2008. | spa |
| dc.relation.references | [76] J. Yin, Y. Xu, H. Yu, P. Zhou y Z. Shen, «Recovery of poly-beta-hydroxybutyrate from recombinant Escherichia coli by combined biologi.chemical method,» Progress in Biotechnology, vol. 16, pp. 213 - 218, 2000. | spa |
| dc.relation.references | [77] G. Schroll, S. Resch, K. Gruber, G. Wanner y W. Lubitz, «Heterologous OX174 gene E-expression in Ralstonia eutropha: E.mediated lysis is not restricted to lambda-subclass of proteobacteria,» Journal of Biotechnology, vol. 66, pp. 211 - 217, 1998. | spa |
| dc.relation.references | [78] J. Mishra, S. Tiwari, M. Abolhasani, S. Azimi y G. Nayak, «2 - Fundamental of polymer blends and its thermodynamics,» de Micro and Nano Fibrillar Composites (MFCs and NFCs) for Polymer Blends, Elsevier, 2017, pp. 27 - 55. | spa |
| dc.relation.references | [79] P. Williams, «Microbial Polyesters: Biosynthesis, Properties, Biodegradation and Applications,» de Renewable Resources for Functional Polymers and Biomaterials, RSC Polymer Chemistry Series, 2011, p. 357. | spa |
| dc.relation.references | [80] M. Niaounakis, «Blending Biopolymers with Synthetic Non-biodegradable Polymers,» de Biopolymers: Processing and Products, Elsevier - PDL Handbook Series, 2014, p. 133. | spa |
| dc.relation.references | [81] P. Viskah, «State of Art, New Challenges and Opportunities,» de Polyhydroxyalkanoate (PHA) based Blends, Composites and Nanocomposites, RSC - Green Chemistry Series, 2014, pp. 1 - 17. | spa |
| dc.relation.references | [82] M. Duarte , R. Goulart Hugen, E. Sant'Anna, A. Testa y S. Pezzin, «Thermal and mechanical behaviour of inyection molded Poly(3-hydroxybutyrate)/Poly(e-caprolactone) blends,» Material Research, vol. 9, 2006. | spa |
| dc.relation.references | [83] S. Godbole, S. Gote, M. Latkar y T. Chakrabarti, «Preparation and characterization of biodegradable poly-3-hydroxybutyrate-starch blend films,» Bioresource Technology, vol. 86, pp. 33 - 37, 2003. | spa |
| dc.relation.references | [84] L. Zhang, C. Xiong y X. Deng, «Miscinility, crystallization and morphology of poly(beta-hydroxybutyrate)/poly(d,l-lactide) blends,» Polymer, vol. 36, pp. 235 - 241, 1996. | spa |
| dc.relation.references | [85] N. Koyama y Y. Doi, «Miscibility of binary blends of poly(R-3-hydroxybutyric acid) and poly((s)-lactic acid),» Polymer, vol. 38, pp. 1589 - 1593, 1998. | spa |
| dc.relation.references | [86] C. Vogel, E. Wessel y H. W. Siesler, «FT-IR Imaging Spectroscopy of Phase Separation in Blends of Poly(3-hydroxybutyrate) with Poly(L-lactic acid) and Poly(e-caprolactone),» Biomacromolecules, vol. 9, pp. 523 - 527, 2007. | spa |
| dc.relation.references | [87] J. Zhang, H. Sato, T. Furukawa, H. Tsuji, I. Noda y Y. Ozaki, «Crystalization behaviors of poly(3-hydroxybutyrate) and poly(l-lactic acid) in their immiscible and miscible blends.,» The Journal of Physical Chemistry B, vol. 110, pp. 24463 - 24471, 2006. | spa |
| dc.relation.references | [88] T. Furukawa, H. Sato y R. Murakami, «Structure, dispersibility, and crystalinity of poly(hydroxybutyrate)/poly(L-lactic acid) blends studied by FT-IR microspectroscopy and differential scanning calorimetry,» Macromolecules, vol. 38, pp. 6445 - 6454, 2005. | spa |
| dc.relation.references | [89] curioseando.com, «¿Qué es el almidón resistente?,» 29 Agosto 2017. [En línea]. Available: https://curiosoando.com/que-es-el-almidon-resistente. [Último acceso: 2019 Marzo 2]. | spa |
| dc.relation.references | [90] L. Yu, Biodegradable polymer blends and composites from renewable resources, New Jersey: Wiley, 2009. | spa |
| dc.relation.references | [91] L. Zhang, X. Deng, S. Zhao y Z. Huang, «Biodegradable polymer blends of poly(3-hydroxybutyrate) and starch acetate,» Polymer International, vol. 44, p. 104, 1997. | spa |
| dc.relation.references | [92] A. M. Ismail y M. A. Gamal, «Water resistance, mechanical properties, and biodegradability of poly(3-hydroxybutyrate)/starch composites,» Journal of Applied Polymer Science, vol. 115, pp. 2813 - 2819, 2010. | spa |
| dc.relation.references | [93] M. Zhang y N. Thomas, «Preparation and properties of polyhydroxybutyrate blended with different types of strach,» Journal of Applied Polymer Science, vol. 16, pp. 688 - 694, 2009. | spa |
| dc.relation.references | [94] P. Pan, L. Zhao, B. Zhu, Y. He y Y. Inoue, «Fractionated Crystalization and Self Nucleation Behaviour of Poly(ethylene oxide) in Its Miscible Blends with Poly(3-hydroxybutyrate),» Journal of Applied Polymer Science, vol. 117, pp. 3013 - 3021, 2009. | spa |
| dc.relation.references | [95] D. Parra, J. Fusaro, F. Gaboardi y D. Rosa, «Influence of poly(ethylene glycol) on the thermal, mechanical, morphological, physical.chemical and biodegradation properties of poly(3-hydroxybutyrate),» Polymer Degradation and Stability, vol. 91, pp. 1954 - 1959, 2006. | spa |
| dc.relation.references | [96] S. Wong, R. Shanks y A. Hodzic, «Properties of poly(3-hydroxybutyric acid) composites with flax fibers modified by plasticiser absorption,» Macromoloecular Materials and Engineering, vol. 287, pp. 647 - 655, 2002. | spa |
| dc.relation.references | [97] M. Khan, K. Ali, G. Hinrichsen, C. Kopp y S. Kropke, «Study on physical and mechanical properties of biopol-jute composite,» Polymer-Plastic Technology and Engineering, vol. 38, pp. 99 - 112, 1999. | spa |
| dc.relation.references | [98] A. Buzarovska, G. Bogoeva-Gaceva, A. Grozdanov, M. Avella, G. Gentile y M. Errico, «Potencial use of rice straw as filler in eco-composite materials,» Australian Journal of Crop Science, vol. 1, pp. 37 - 42, 2008. | spa |
| dc.relation.references | [99] H.-Y. Qin, Z.-Y. Liu, Y.-N. Chen, L. Liu y N. Zhou, «Simultaneous improvement of mechanical properties and thermal stability of bacterial polyester by cellulose nanocrystals,» Carbohydrate Polymers, vol. 89, pp. 971 - 978, 2012. | spa |
| dc.relation.references | [100] E. Ten, L. Jiang y M. Wolcott, «Preparation and properties of aligned poly(3-hydroxybutyrate-co-3hydroxyvalerate)/cellulose nanowhiskers composites,» Carbohydrate Polymers, vol. 92, pp. 206 - 213, 2013. | spa |
| dc.relation.references | [101] Y. Srithep, T. Ellingham, J. Peng, R. Sabo, C. Clemons, L. Turng y S. Pilla, «Melt compounding of poly(3-hydroxybutyrate-co-2hydroxyvalerate)/nanofibrillated cellulose nancomposites,» Polymer Degradation and Stability, vol. 98, pp. 1439 - 1449, 2013. | spa |
| dc.relation.references | [102] U. Bhardwaj, P. Dhar, A. Kumar y V. Katiyar, «Polyhydroxyalkanoates (PHA)-Cellulose Based Nanobiocomposites for Food Packaging Applications,» Food Additives and Packaging, pp. 276 - 314, 2014. | spa |
| dc.relation.references | [103] D. Florez, Z. Suarez, N. Merchan, L. Gutierrez, L. Olmos y N. Moreno-Sarmiento, «Mutagenesis studies for enhancement of PHA production by Burkholderia cepacia 2G57, isolated from colombian soils,» Santos Brasil: International Symposium of Biopolymers , 2014. | spa |
| dc.relation.references | [104] A. Viloria, K. Ardila, D. Mendez, I. Cabeza y N. Moreno, «Volumetric Oxygen Mass Transfer Coefficient Determination and Hydrodynamic Optimization of Polyhydroxyalkanoate Prodution with Vegetal Oil as Carbon Source,» Chemical Engineering Transactions, vol. 57, 2017. | spa |
| dc.relation.references | [105] F. J., «Extracción de PHAs con SDS,» Instituto de Biotecnología de la Universidad Nacional de Colombia, Bogotá., 2016. | spa |
| dc.relation.references | [106] A. Garzón y D. Villamizar, «Purificación y cracaterización de biopolímero tipo polihidroxialcanoato (PHA) - (Informe de Pasantía),» Instituto de Biotecnología de la Universidad Nacional, Bogotá, 2017. | spa |
| dc.relation.references | [107] A. Rosengart, M. Cesário, M. de Almeida, R. Raposo, S. Espert, A. de Apodaca y M. da Fonseca , «Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents,» Biochemical Engineering Journal, vol. 103, pp. 39 - 46, 2015. | spa |
| dc.relation.references | [108] T. Fei, «Effective recovery of Poly-b-hydroxybutyrate (PHB) biopolymer from Cupriavidus necator using novel and environmentaly friendly solvent system.,» Biotechnology Progress 32, pp. 678 - 685, 2016. | spa |
| dc.relation.references | [109] P. Hoftyzer y D. W. Van Krevelen, Properties of polymers - 4 ed, Amsterdam: ELSEVIER, 2009. | spa |
| dc.relation.references | [110] G. Braunegg, G. Lefebvre y K. F. Genser, «Polyhydroxyalkanoates, biopolyester from renewable resources: Physiological and engineering aspects,» Journal of Biotechnology 65, pp. 127 - 161, 1998. | spa |
| dc.relation.references | [111] L. Riedel, C. Brigham, C. Budde, J. Bader, C. Rha, U. Stahl y A. Sinskey, «Recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutropha cultures with non-halogenated solvents,» Biotechnology and Bioengineering 30, pp. 1 - 9, 2012. | spa |
| dc.relation.references | [112] A. Aramvash, Z. Moazzeni y B. Gholami, «Comparision of different solvents for extraction of polyhydroxybutyrate from Cupriavidus necator,» Engineering and Life Sciences - 18 , pp. 20 - 28, 2018. | spa |
| dc.relation.references | [113] A. Rosengart, M. T. Cesario, M. C. de Almeida, R. Raposo, A. Espert, E. Diaz de Apodaca y M. da Fonseca, «Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents,» Biochemical Engineering Journal, pp. 39 - 46, 2015. | spa |
| dc.relation.references | [114] K. Kinoshita, F. Osakada, Y. Ueda, K. Narasimhan, A. Cearley, K. Yee y I. Noda.Estados Unidos Patente US7098298B2, 2003. | spa |
| dc.relation.references | [115] E. B. Bagley, T. P. Nelson y J. M. Scigliano, «Three-dimensional solubility parameters and their relationship to internal pressure measurements in polar and hydrogen bonding solvents,» Journal of Paint Technology , p. 43, 197. | spa |
| dc.relation.references | [116] J. D. Hayler , A. M. Redman, R. K. Henderson , C. M. Alder, L. Shukla, L. Shuster y H. Sneddon, «Updating and further expanding GSK´s solvent sustentability guide,» Green Chemistry 18, pp. 3879 - 3890, 2016. | spa |
| dc.relation.references | [117] A. Tejada, R. Montesinos y R. Guzman, Bioseparaciones 2da Edición, Ciudad de México: Pearson, 2011. | spa |
| dc.relation.references | [118] R. H. Marchessault, K. Okamura y C. J. Su, «Physical Properties of Poly (b-hidroxybutyrate). II. Conformational Aspects in Solution,» Macromolecules, pp. 735-740, 1970. | spa |
| dc.relation.references | [119] S. Akita, Y. Einaga, Y. Miyaki y H. Fujita, «Solution Properties of Poly (D-B-hydroxybutyrate). 1. Biosynthesis and Characterization». | spa |
| dc.relation.references | [120] J. E. Mark, Polymer Data Handbook, Oxford: Oxford University, 1999. | spa |
| dc.relation.references | [121] P. Huang, S. Furutate, S. Mizuno y T. Tsuge, «Thermal degradation behavior of bacterial poly(3-hydroxybutyrate-co-3-mercaptopropionate),» Polymer Degradation and Stability, vol. 165, pp. 35 - 42, 2019. | spa |
| dc.relation.references | [122] M. Akodogan y E. Celik, «Purification and characterization of polyhydroxyalkanoate (PHA) from a Bacillus megaterium strain using various dehydration techniques,» Journal of Chemical Technology and Biotechnology, vol. 8, pp. 2292 - 2298, 2018. | spa |
| dc.relation.references | [123] C. Chan, P. Johansson, P. Magnusson, L. -. J. Vandi, M. Hernandez, P. Halley, B. Laycock, S. Pratt y A. Werker, «Mixed culture polyhydroxyalkanoate-rich biomass assessment and quality controlusing thermogravimetric measurement methods,» Polymer Degradation and Estability, vol. 17, 2017. | spa |
| dc.relation.references | [124] N. Grassie, E. Murry y P. Holms, «The thermal degradation of poly(-(D)-b-hydroxybutyric acid): part 3- the reaction mechanism.,» Polymer Degradation and Stability, vol. 127, nº 34, p. 6, 1984. | spa |
| dc.relation.references | [125] R. Lehrle y R. Williams, «Thermal degradation of bacterial poly(hydroxybutyricacid): mechanism from the dependence of pyrolysis yields on sample thick-ness,» Macromolecules, vol. 27, nº 9, 1994. | spa |
| dc.relation.references | [126] R. Lehrle, R. Williams, C. French y T. Hammond, «Thermolysis and methanolysisof poly(b-hydroxybutyrate): random scission assessed by statistical analysisof molecular weight distributions,» Macromolecules, vol. 28, nº 14, p. 4408, 1995. | spa |
| dc.relation.references | [127] M. Chan Sin, S. Neon Gan, M. S. Mohd Annuar y I. K. Ping Tan, «Thermodegradation of medium-chain-length poly(3-hydroxyalkanoates)produced byPseudomonas putidafrom oleic acid,» Polymer Degradation and Stability, vol. 95, pp. 2334 - 2342, 2010. | spa |
| dc.relation.references | [128] S. Mohandas, L. Balan, N. Lekshmi, S. S. Cubelio, R. Philip y I. S. Birght Singh, «Production and characterization of polyhydroxybutyrate from Vibrio harveyi MCBB 284 utilizing glycerol as carbon source,» Journal of Applied Microbiology, vol. 122, nº 3, pp. 698 - 707, 2017. | spa |
| dc.relation.references | [129] S. Bhattacharya, S. Dubey, P. Singh, A. Shrivastava y S. Mishra, «Biodegradable Polymeric Substances Produced by a Marine Bacterium from Surplus Stream of the Biodiesel Industry,» Bioengineering, vol. 3, nº 34, pp. 3 - 11, 2016. | spa |
| dc.relation.references | [130] M. Ganesh, A. Senthamari, S. Shanmughapriya y K. Natarajaseenivasan, «Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source,» Bioresource Technology, vol. 192, pp. 677 - 681, 2015. | spa |
| dc.relation.references | [131] E. Hernández - Nuñez, C. A. Martinez - Gutierrez, A. López - Cortés, M. L. Aguirre - Macedo, C. Tabasco - Novelo, M. O. González - Díaz y J. Q. Garcia Maldonado, «Physico-chemical Characterization of Poly(3-Hydroxybutyrate) Produced by Halomonas salina, Isolated from a Hypersaline Microbial Mat,» Journal of Polymers and the Environment, 2019. | spa |
| dc.relation.references | [132] N. C. Loureiro, S. Ghosh, J. C. Viana y J. L. Esteves, «Thermal Characterization of Polyhydroxyalkanoates and Poly(lactic acid) Blends Obtained by Injection Molding,» Polymer Plastics Technology and Engineering, vol. 54, pp. 350 - 356, 2015. | spa |
| dc.relation.references | [133] L. Wei y A. G. McDonald, «Thermophysical properties of bacterial poly(3-hydroxybutyrate): Characterized by TMA, DSC, and TMDSC,» Journal of Applied Polymer Science, vol. 42412, pp. 1 - 11, 2015. | spa |
| dc.relation.references | [134] A. Modeli, B. Calcagno y M. Scandola, «Kinetics of Aerobic Polymer Degradation in Soil by Means of the ASTM D 5988-96 Standard Method,» Journal of Environmental Polymer Degradation, pp. 109-116, 1999. | spa |
| dc.relation.references | [135] H. Chen, Assessment of Biodegradation in Different Environmental Compartments of Blends and Composites Based On Microbial Poly(hydroxialkanoates), Pisa: University of Pisa, 2012. | spa |
| dc.relation.references | [136] D. Parra, J. Fusaro, F. Gaboardi y D. Rosa, «Influence of Poly(ethylene glycol) on the thermal, mechanical, morphological, physical-chemical and biodegradation properties of poly(3-hydroxybutyrate),» Polymer Degradation and Stability, pp. 1954-1959, 2006. | spa |
| dc.relation.references | [137] D. Panaitescu, C. Nicolae, A. Frone, I. Chiulan, P. Stanescu, C. Draghici, M. Iorga y M. Mihailescu, «Plasticized P3HB with improved processing and balanced properties,» Journal of Applied Polymer Science, 2016. | spa |
| dc.relation.references | [138] D. L. Alberti, O. F. Souza, I. Barcellos y D. Z. Bucci, «Study on Physical and Mechanical Properties of PHB Biocomposites with Rice Hull Ash,» Materials and Science Forums , pp. 557 - 561, 2014. | spa |
| dc.relation.references | [139] Y. B. Kim y R. W. Lenz, «Polyesters from Microorganisms,» de Biopolyesters, Münster - Alemania, Springer, 2001, pp. 51 - 79. | spa |
| dc.relation.references | [140] K. Hong, S. Sun, W. Tian, G. Chen y W. Huang, «A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy,» Applied Microbiology and Biotechnology, vol. 51, pp. 523 - 526, 1999. | spa |
| dc.relation.references | [141] R. Majumdar, K. Alexander y A. Rigia, «Physical characterization of polyethylene glycols by thermal analytical technique and the effect of humidity and molecular weigth,» Pharmazie, vol. 65, pp. 343 - 347, 2010. | spa |
| dc.relation.references | [142] D. Trache, A. Donnot, K. Khimeche, R. Benelmir y N. Brosse, «Physicho-chemical properties and thermal stability of microcrystalline cellulose isolated from Alfa fibres,» Carbohydrate Polymers, vol. 104, pp. 223 - 230, 2014. | spa |
| dc.relation.references | [143] M. A. Abdelwahab, A. Flynn, B. S. Chiou, S. Imam y W. Orts, «Thermal, mechanical and morphological characterization of plasticized PLA - PHB blends,» Polymer degradation and stability, vol. 97, pp. 1822 - 1828, 2012. | spa |
| dc.relation.references | [144] J. C. Alzate Marin, S. Rivero , A. Pinotti y H. A. Caravelli, «Microstructural behaviour of matrices based on polylactic acid and polyhydroxyalkanoates,» Agricultural and food chemistry, pp. 1 - 34, 2018. | spa |
| dc.relation.references | [145] P. Tri Nguyen, S. Domenek, A. Guinault y C. Sollogub, «Cristalization Behaviour of Poly(lactide)/Poly(b-hydroxybutyrate)/Talc Composites,» Applied Polymer Science, vol. 129, pp. 3355 - 3365, 2013. | spa |
| dc.relation.references | [146] N. Yoshie, K. Nakasato, M. Fujiwara , K. Kasuya, H. Abe y Y. Doi, «Effect of low molecular weight additives on enzymatic degradation of poly(3-hydroxybutyrate),» Polymer, vol. 9, pp. 3227 - 3234, 2000. | spa |
| dc.relation.references | [147] G. Uzun y D. Aydemir, «Biocomposites from polyhydroxybutyrate and bio-fillers by solvent casting method,» Bulletin of Material Science, 2016. | spa |
| dc.relation.references | [148] U. Bhardwaj, P. Dhar, A. Kumar y V. Katiyar, «Polyhydroxyalkanoates(PHA) - cellulose based nanocomposites for food packaging applications,» de Food Additives and Packaging , Washington DC, ASC Symposium Series - American Chemical Society, 2014, pp. 276 - 314. | spa |
| dc.relation.references | [149] V. Jost y C. Langowski, «Effect of different plasticizers on the mechanical and barrier properties of extruded cast PHBV films,» European Polymer Journal, vol. 68, pp. 302 - 312, 2015. | spa |
| dc.relation.references | [150] J. Hildebrand y R. Scott, Regular Solutions, NJ: Prentice Hall, 1962. | spa |
| dc.relation.references | [151] C. Hansen, «The three dimensional solubility parameter - key to paint component affinities,» Journal of Paint Technology, pp. 1 - 8, 1967. | spa |
| dc.relation.references | [152] C. Hansen, Hansel Solubility Parameters: A user´s Handbook - 2 ed, Londres: CRC Press, 2007. | spa |
| dc.relation.references | [153] I. Noda, R. Marchessault y M. Terada, Polymer Data Handbook, Oxford: Oxford University Press, 1999. | spa |
| dc.relation.references | [154] L. Mortino, M. V. Cruz, A. Scoma, F. Freitas, L. Bertin, M. Scandolla y M. A. Reis, «Recovery of amorphus polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil,» International Journal of Biological Macromolecules, vol. 70, pp. 117 - 123, 2014. | spa |
| dc.relation.references | [155] H. Tsuji y I. Fukui, «Enhanced thermal stability of poly(lactides) in the melt by enantiomeric polymer blending,» Polymer, vol. 44, pp. 2891 - 2896, 2003. | spa |
| dc.rights | Derechos reservados - Universidad Nacional de Colombia | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.license | Atribución-NoComercial 4.0 Internacional | spa |
| dc.rights.spa | Acceso abierto | spa |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | spa |
| dc.subject.ddc | 660 - Ingeniería química | spa |
| dc.subject.proposal | Poli(3-hidroxibutirato) | spa |
| dc.subject.proposal | Poly(3-hydroxybutyrate) | eng |
| dc.subject.proposal | Burkholderia cepacia | spa |
| dc.subject.proposal | Burkholderia cepacia | eng |
| dc.subject.proposal | extracción con solvente | spa |
| dc.subject.proposal | Solvent extraction | eng |
| dc.subject.proposal | Polylactic acid | eng |
| dc.subject.proposal | ácido poliláctico | spa |
| dc.subject.proposal | biopolímeros | spa |
| dc.subject.proposal | Biopolymers | eng |
| dc.subject.proposal | mezclas poliméricas | spa |
| dc.subject.proposal | Polymeric blends | eng |
| dc.title | Extracción, purificación y modificación de un biopolímero del tipo poli (3-hidroxibutirato) obtenido de la fermentación de ácidos grasos con B. cepacia. | spa |
| dc.title.alternative | Extraction, purification and modification of a poly (3-hydroxybutyrate) type biopolymer obtained from the fermentation of fatty acids with B. cepacia | spa |
| dc.type | Trabajo de grado - Maestría | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
| dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | spa |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |