Tratamiento de Mycoplasma sp. presente en lineas celulares animales
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Ferrucho Calle, Maria Camila
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Abstract
Cell cultures in the pharmaceutical and cosmetic industry require strict control of parameters,
including contamination by Mycoplasma sp., due to its negative effects on economic and
biological aspects. In this study, the treatment to eradicate Mycoplasma sp. in contaminated L929
cells was evaluated. Three detection methods (PCR, biochemical luminescence, and DAPI
staining) were used to efficiently screen for the presence of Mycoplasma sp., and the necessary
concentration of the treatment was determined. In addition, the growth rate of the evaluated
cells was compared, and a viability assay was performed with different concentrations of SDS
to analyze their response after treatment.
During the study, contamination of L929 cells with Mycoplasma sp. was detected, and the
effectiveness of the treatments with Plasmocin®
and MRA to eradicate the pathogen was
confirmed. However, despite the effectiveness of the treatments, the presence of Mycoplasma sp.
caused minimal changes in the growth rate of the evaluated cells, particularly between 120 and
144 hours of study. In this period, a decrease in the growth rate was observed in the cells treated
with MRA, while those treated with Plasmocin®
managed to recover their growth rate.
Furthermore, in the cell viability assay, the treatment appeared to provide additional protection
against the cytotoxic effect, especially at the lower concentrations of SDS that were evaluated.
However, slight changes in cell morphology were observed, indicating irreversible alterations
due to contamination with Mycoplasma sp. despite the efficacy of the treatment.
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