Estudio comparativo de la morfología ósea, en individuos prehispánicos sanos y con treponematosis, utilizando tecnología de punta
Authors
Castañeda Murcia, Zoila Emilia
Director
Contreras Villa, Dimas Dennis (Thesis advisor)
Florido Caicedo, Carlos Arturo (Thesis advisor)
Content type
Trabajo de grado - Maestría
Document language
EspañolPublication date
2011
Journal Title
Journal ISSN
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Abstract
Este trabajo de investigación plantea la posibilidad de utilizar tecnología de punta en el diagnóstico paleopatológico como una herramienta de alta complejidad y mayor discriminación, en el diagnóstico de la microestructura de los huesos con periostitis, frente a un control normal. Se utilizaron secciones pequeñas de tibias y fémur de individuos prehispánicos normales y con periostitis, frente a muestras de individuos contemporáneos. Se realizo un análisis morfológico macroscópico, radiográfico, histológico convencional por técnica de desgaste, microscopia Confocal laser (MCLB) y microscopio electrónico de barrido (MEB). Los resultados obtenidos muestran la respuesta tanto perióstica como endostica del hueso afectado y la presencia de células momificadas, la preservación del colágeno así como la identificación y análisis iónico de elementos biogénicos, demostrando el beneficio de las técnicas de MEB y MCLB como herramienta de identificación en restos humanos de individuos prehispánicos.
Abstract. This research raises the possibility of using technology in the diagnosis paleopathological as a tool of high complexity and greater discrimination in the diagnosis of bone microstructure with periostitis, compared with normal control. We used small sections of tibia and femur of normal and pre-Hispanic individuals periostitis, compared to samples of contemporary individuals. Morphological analysis was performed macroscopic, radiographic, histological wear conventional technique, confocal laser microscopy (MCLB) and scanning electron microscope (SEM). The results obtained show the periosteal and endosteal response to both the affected bone and mummified cells are present, the preservation of collagen and ion identification and analysis of biogenic elements, demonstrating the benefit of SEM techniques and MCLB as an identification tool in human remains of prehispanic individuals.
Abstract. This research raises the possibility of using technology in the diagnosis paleopathological as a tool of high complexity and greater discrimination in the diagnosis of bone microstructure with periostitis, compared with normal control. We used small sections of tibia and femur of normal and pre-Hispanic individuals periostitis, compared to samples of contemporary individuals. Morphological analysis was performed macroscopic, radiographic, histological wear conventional technique, confocal laser microscopy (MCLB) and scanning electron microscope (SEM). The results obtained show the periosteal and endosteal response to both the affected bone and mummified cells are present, the preservation of collagen and ion identification and analysis of biogenic elements, demonstrating the benefit of SEM techniques and MCLB as an identification tool in human remains of prehispanic individuals.