Desarrollo de una herramienta para caracterización palinológica por medio de microscopı́a holográfica digital
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Torres Rodríguez, Edgar Mauricio
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En palinologíaa, los biólogos utilizan el tama~no y la forma como parámetros para la clasificación de los granos de polen, sin embargo la Microscopía Holográfica Digital (MHD) y la Tomografía óptica (TO) pueden aportar otros paráametros de caracterización como lo son: el índice de refracción y el espesor de las muestras en estudio. El campo óptico complejo transmitido de muestras de polen es obtenido a través de hologramas de transmisión para cien planos diferentes, barriendo 180° y obteniendo información de cada plano de la muestra. Se desarrolló una estrategia de análisis, que a partir de dichos campos complejos, permitiera separar la información de espesores e índice de refracción interno de la muestra en estudio. Esta información 2D se usó como insumo para la implementación de un sistema de Tomografía óptica. Se obtuvieron los mapas 3D de espesores y los mapas 3D de índices relativos de refracción de las muestras en estudio, a esta técnica combinada de MHD y TO se le conoce con el nombre de Microtomografía óptica (MTO).
Abstract. In palynology the biologists use the size and shape as parameters for the classification of the pollen grains, however the Digital Holography Microscopy (DHM) and Optics Tomography (TO) can provide other characterization parameters such as: the refractive index and the topography of the samples in study. The optical complex field is obtained thru transmission holograms for a hundred different planes, covering 180 degrees and reaching every point of the sample. A strategy for the analysis of the obtained complex field was developed to separate the information about the thickness and the internal refractive index of the sample studied. This 2D information was used as input for the implementation of an optical tomography system, 3D maps of thickness and 3D maps of relative index refraction were obtained from the samples in study, this technique combined the MHD and OT, and it is known as Optical Microtomography (MTO).
Abstract. In palynology the biologists use the size and shape as parameters for the classification of the pollen grains, however the Digital Holography Microscopy (DHM) and Optics Tomography (TO) can provide other characterization parameters such as: the refractive index and the topography of the samples in study. The optical complex field is obtained thru transmission holograms for a hundred different planes, covering 180 degrees and reaching every point of the sample. A strategy for the analysis of the obtained complex field was developed to separate the information about the thickness and the internal refractive index of the sample studied. This 2D information was used as input for the implementation of an optical tomography system, 3D maps of thickness and 3D maps of relative index refraction were obtained from the samples in study, this technique combined the MHD and OT, and it is known as Optical Microtomography (MTO).