Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19

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dc.contributor.advisorPinzón Velasco, Andrés Mauricio
dc.contributor.authorHernández Bocanegra, Natalia Andrea
dc.contributor.orcidhttps://orcid.org/0009-0001-4161-6069spa
dc.contributor.researchgroupBioinformáticaspa
dc.coverage.cityBogotá, Colombia
dc.coverage.temporal2020-2021
dc.date.accessioned2024-07-16T15:25:52Z
dc.date.available2024-07-16T15:25:52Z
dc.date.issued2024
dc.descriptionIlustraciones a color, diagramasspa
dc.description.abstractThe interaction between the Spike protein of SARS-CoV-2 and the human ACE2 receptor plays a pivotal role in the development of COVID-19. This study aimed to assess the genetic variability of the viral lineage and seven exons of the human ACE2 gene, along with analyzing clinical backgrounds and sociodemographic conditions related to susceptibility and disease severity. Using sequencing technology, samples from nasopharyngeal swabs were examined for the viral genome, and blood samples from 50 individuals diagnosed with SARS-CoV-2 infection or viral pneumonia were sequenced. Although no statistically significant associations were found between viral lineages and disease severity, it was observed that the B.1.621 lineage predominated in mild cases, while the B.1.420 lineage was present in moderate cases, and the P.1 lineage was found in both moderate and severe individuals. Analysis of exonic sequences of the ACE2 gene revealed a homozygous variant in exon 17, involving a synonymous substitution at position 133, resulting in the encoding of valine. No significant associations were found between demographic data, clinical history, and disease severity. These results indicate genetic variability in the studied regions of the ACE2 gene, underscoring the importance of future large-scale studies with representative samples to comprehend the virus's pathophysiology and its relationship with the developed phenotype.eng
dc.description.abstractLa interacción entre la proteína Spike del SARS-CoV-2 y el receptor ACE2 humano es crucial en el desarrollo de la enfermedad COVID-19. Este estudio buscó evaluar la variabilidad genética del linaje viral y de siete exones del gen ACE2 humano, así como analizar antecedentes clínicos y condiciones sociodemográficas relacionadas con la susceptibilidad y severidad de la enfermedad. Utilizando tecnología de secuenciación, se examinaron muestras de hisopado nasofaríngeo para el genoma viral y muestras sanguíneas de 50 individuos diagnosticados con infección o neumonía viral por SARS-CoV-2. Estos individuos fueron ingresados a la Clínica Universitaria Colombia, ubicada en la ciudad de Bogotá, desde octubre del año 2020 a junio del año 2021, y se clasificaron en tres grupos de severidad según los síntomas, el diagnóstico, la insuficiencia respiratoria y la necesidad de ventilación como: leve, moderado y severo. A pesar de no encontrar asociaciones estadísticamente significativas entre los linajes virales y la gravedad de la enfermedad, se observó que el linaje B.1.621 predomina en casos leves, mientras que el linaje B.1.420 está presente solo en casos moderados y el linaje P.1 se encontró en individuos moderados y severos. El análisis de las secuencias de los siete exones del gen ACE2 reveló una única variante homocigota en el exón 17; que corresponde a la sustitución de una guanina (G) por una adenina (A), causando un cambio sinónimo en la posición 748 de la proteína ACE2 que resulta en la codificación de una valina. No se encontraron correlaciones significativamente estadísticas entre datos demográficos, antecedentes clínicos y gravedad de la enfermedad. Se requieren estudios futuros con muestras representativas para comprender la fisiopatología del virus y su relación con el fenotipo desarrollado. (Texto tomado de la fuente)spa
dc.description.degreelevelMaestríaspa
dc.description.researchareaBiología molecular de agentes infecciososspa
dc.format.extentviii, 71 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/86453
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
dc.relation.referencesAbdullaev, A., Abdurakhimov, A., Mirakbarova, Z., Ibragimova, S., Tsoy, V., Nuriddinov, S., Dalimova, D., Turdikulova, S., & Abdurakhmonov, I. (2022). Genome sequence diversity of SARS-CoV-2 obtained from clinical samples in Uzbekistan. PLoS ONE, 17(6). https://doi.org/10.1371/JOURNAL.PONE.0270314spa
dc.relation.referencesÁlvarez-Díaz, D. A., Ruiz-Moreno, H. A., Zapata-Bedoya, S., Franco-Muñoz, C., Laiton-Donato, K., Ferro, C., Sepulveda, M. T. H., Pacheco-Montealegre, M., Walteros, D. M., Carrero-Galindo, L. C., & Mercado-Reyes, M. (2022). Clinical outcomes associated with Mu variant infection during the third epidemic peak of COVID-19 in Colombia. International Journal of Infectious Diseases, 125, 149–152. https://doi.org/10.1016/j.ijid.2022.10.028spa
dc.relation.referencesAngulo-Aguado, M., Corredor-Orlandelli, D., Carrillo-Martínez, J. C., Gonzalez-Cornejo, M., Pineda-Mateus, E., Rojas, C., Triana-Fonseca, P., Contreras Bravo, N. C., Morel, A., Parra Abaunza, K., Restrepo, C. M., Fonseca-Mendoza, D. J., & Ortega-Recalde, O. (2022). Association Between the LZTFL1 rs11385942 Polymorphism and COVID-19 Severity in Colombian Population. Frontiers in Medicine, 9, 910098. https://doi.org/10.3389/FMED.2022.910098/FULLspa
dc.relation.referencesAuton, A., Abecasis, G. R., Altshuler, D. M., Durbin, R. M., Bentley, D. R., Chakravarti, A., Clark, A. G., Donnelly, P., Eichler, E. E., Flicek, P., Gabriel, S. B., Gibbs, R. A., Green, E. D., Hurles, M. E., Knoppers, B. M., Korbel, J. O., Lander, E. S., Lee, C., Lehrach, H., … Schloss, J. A. (2015). A global reference for human genetic variation. Nature, 526(7571), 68. https://doi.org/10.1038/NATURE15393spa
dc.relation.referencesBenetti, E., Tita, R., Spiga, O., Ciolfi, A., Birolo, G., Bruselles, A., Doddato, G., Giliberti, A., Marconi, C., Musacchia, F., Pippucci, T., Torella, A., Trezza, A., Valentino, F., Baldassarri, M., Brusco, A., Asselta, R., Bruttini, M., Furini, S., … Pinto, A. M. (2020). ACE2 gene variants may underlie interindividual variability and susceptibility to COVID-19 in the Italian population. European Journal of Human Genetics, 28(11), 1602–1614. https://doi.org/10.1038/s41431-020-0691-zspa
dc.relation.referencesBigDye ® Terminator v3.1 Cycle Sequencing Kit Protocol. (2002).spa
dc.relation.referencesBiti, R., French, R. F., Young, J., Bennetts, B., Stewart, G., & Liang, T. (1997). HIV-1 infection in an individual homozygous for the CCR5 deletion allele. Nature Medicine, 3(3), 252–253. https://doi.org/10.1038/NM0397-252spa
dc.relation.referencesCaicedo, J. D., Cáceres, A., Arboleda-Bustos, C. E., Mahecha, M. F., Ortega, J., Arboleda, G., & Arboleda, H. (2019). Genetic variability analysis in a population from Bogota: Toards a haplotype map. Biomedica, 39(3). https://doi.org/10.7705/BIOMEDICA.4753spa
dc.relation.referencesCandido, D. S., Claro, I. M., de Jesus, J. G., Souza, W. M., Moreira, F. R. R., Dellicour, S., Mellan, T. A., du Plessis, L., Pereira, R. H. M., Sales, F. C. S., Manuli, E. R., Thézé, J., Almeida, L., Menezes, M. T., Voloch, C. M., Fumagalli, M. J., Coletti, T. M., da Silva, C. A. M., Ramundo, M. S., … Faria, N. R. (2020). Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science (New York, N.y.), 369(6508), 1255–1260. https://doi.org/10.1126/SCIENCE.ABD2161spa
dc.relation.referencesCasalino, L., Gaieb, Z., Goldsmith, J. A., Hjorth, C. K., Dommer, A. C., Harbison, A. M., Fogarty, C. A., Barros, E. P., Taylor, B. C., Mclellan, J. S., Fadda, E., & Amaro, R. E. (2020). Beyond shielding: The roles of glycans in the SARS-CoV-2 spike protein. ACS Central Science, 6(10), 1722–1734. https://doi.org/10.1021/ACSCENTSCI.0C01056/SUPPL_FILE/OC0C01056_SI_006.ZIPspa
dc.relation.referencesCastillo, A. E., Parra, B., Tapia, P., Acevedo, A., Lagos, J., Andrade, W., Arata, L., Leal, G., Barra, G., Tambley, C., Tognarelli, J., Bustos, P., Ulloa, S., Fasce, R., & Fernández, J. (2020). Phylogenetic analysis of the first four SARS‐CoV‐2 cases in Chile. Journal of Medical Virology, 92(9), 1562. https://doi.org/10.1002/JMV.25797spa
dc.relation.referencesCentro para el control y la prevención de enfermedades. (n.d.). Clasificaciones y definiciones de las variantes del SARS-CoV-2.spa
dc.relation.referencesClinical Spectrum | COVID-19 Treatment Guidelines. (n.d.). Retrieved January 5, 2024, from https://www.covid19treatmentguidelines.nih.gov/overview/clinical-spectrum/spa
dc.relation.referencesCurtis, D. (2021). Variants in ACE2 and TMPRSS2 Genes Are Not Major Determinants of COVID-19 Severity in UK Biobank Subjects. Human Heredity, 85(2), 66–68. https://doi.org/10.1159/000515200spa
dc.relation.referencesDeng, S. Q., & Peng, H. J. (2020). Characteristics of and public health responses to the coronavirus disease 2019 outbreak in China. In Journal of Clinical Medicine (Vol. 9, Issue 2). MDPI. https://doi.org/10.3390/jcm9020575spa
dc.relation.referencesDevaux, C. A., Rolain, J. M., & Raoult, D. (2020). ACE2 receptor polymorphism: Susceptibility to SARS-CoV-2, hypertension, multi-organ failure, and COVID-19 disease outcome. In Journal of Microbiology, Immunology and Infection (Vol. 53, Issue 3, pp. 425–435). Elsevier Ltd. https://doi.org/10.1016/j.jmii.2020.04.015spa
dc.relation.referencesDieter, C., Brondani, L. de A., Leitão, C. B., Gerchman, F., Lemos, N. E., & Crispim, D. (2022). Genetic polymorphisms associated with susceptibility to COVID-19 disease and severity: A systematic review and meta-analysis. PLOS ONE, 17(7), e0270627. https://doi.org/10.1371/JOURNAL.PONE.0270627spa
dc.relation.referencesEllinghaus, D., Degenhardt, F., Bujanda, L., Buti, M., Albillos, A., Invernizzi, P., Fernández, J., Prati, D., Baselli, G., Asselta, R., Grimsrud, M. M., Milani, C., Aziz, F., Kässens, J., May, S., Wendorff, M., Wienbrandt, L., Uellendahl-Werth, F., Zheng, T., … Karlsen, T. H. (2020). Genomewide Association Study of Severe Covid-19 with Respiratory Failure. New England Journal of Medicine, 383(16), 1522–1534. https://doi.org/10.1056/NEJMoa2020283spa
dc.relation.referencesFaria, N. R., Mellan, T. A., Whittaker, C., Claro, I. M., da Candido, D. S., Mishra, S., E Crispim, M. A., S Sales, F. C., Hawryluk, I., McCrone, J. T., G Hulswit, R. J., M Franco, L. A., Ramundo, M. S., de Jesus, J. G., Andrade, P. S., Coletti, T. M., Ferreira, G. M., M Silva, C. A., Manuli, E. R., … Sabino, E. C. (n.d.). Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil. In Mélodie Monod (Vol. 25). http://pangolin.cog-uk.iospa
dc.relation.referencesFehr, A. R., & Perlman, S. (2015). Coronaviruses: An overview of their replication and pathogenesis. Coronaviruses: Methods and Protocols, 1282, 1–23. https://doi.org/10.1007/978-1-4939-2438-7_1/TABLES/2spa
dc.relation.referencesGISAID - gisaid.org. (n.d.). Retrieved December 7, 2023, from https://gisaid.org/spa
dc.relation.referencesGlowacka, I., Bertram, S., Müller, M. A., Allen, P., Soilleux, E., Pfefferle, S., Steffen, I., Tsegaye, T. S., He, Y., Gnirss, K., Niemeyer, D., Schneider, H., Drosten, C., & Pöhlmann, S. (2011a). Evidence that TMPRSS2 Activates the Severe Acute Respiratory Syndrome Coronavirus Spike Protein for Membrane Fusion and Reduces Viral Control by the Humoral Immune Response. Journal of Virology, 85(9), 4122–4134. https://doi.org/10.1128/jvi.02232-10spa
dc.relation.referencesGómez, S. A., Rojas-Valencia, N., Gómez, S., Cappelli, C., & Restrepo, A. (2021). The Role of Spike Protein Mutations in the Infectious Power of SARS-COV-2 Variants: A Molecular Interaction Perspective. Chembiochem : A European Journal of Chemical Biology. https://doi.org/10.1002/cbic.202100393spa
dc.relation.referencesGorbalenya, A. E., Baker, S. C., Baric, R. S., de Groot, R. J., Drosten, C., Gulyaeva, A. A., Haagmans, B. L., Lauber, C., Leontovich, A. M., Neuman, B. W., Penzar, D., Perlman, S., M Poon, L. L., Samborskiy, D. V, Sidorov, I. A., Sola, I., & Ziebuhr, J. (n.d.). The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nature Microbiology. https://doi.org/10.1038/s41564-020-0695-zspa
dc.relation.referencesGorbalenya, A. E., Baker, S. C., Baric, R. S., de Groot, R. J., Drosten, C., Gulyaeva, A. A., Haagmans, B. L., Lauber, C., Leontovich, A. M., Neuman, B. W., Penzar, D., Perlman, S., Poon, L. L. M., Samborskiy, D. V., Sidorov, I. A., Sola, I., & Ziebuhr, J. (2020). The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nature Microbiology, 5(4), 536–544. https://doi.org/10.1038/S41564-020-0695-Zspa
dc.relation.referencesGorbalenya, A. E., Baker, S. C., Baric, R. S., de Groot, R. J., Drosten, C., Gulyaeva, A. A., Haagmans, B. L., Lauber, C., Leontovich, A. M., Neuman, B. W., Penzar, D., Perlman, S., Poon, L. L., Samborskiy, D., Sidorov, I. A., Sola, I., & Ziebuhr, J. (n.d.). Severe acute respiratory syndrome-related coronavirus: The species and its viruses-a statement of the Coronavirus Study Group. https://doi.org/10.1101/2020.02.07.937862spa
dc.relation.referencesGordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O’Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Hüttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., … Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature, 583(7816), 459–468. https://doi.org/10.1038/s41586-020-2286-9spa
dc.relation.referencesGreaney, A. J., Loes, A. N., Crawford, K. H. D., Starr, T. N., Malone, K. D., Chu, H. Y., & Bloom, J. D. (2021). Comprehensive mapping of mutations in the SARS-CoV-2 receptor-binding domain that affect recognition by polyclonal human plasma antibodies. Cell Host & Microbe, 29(3), 463. https://doi.org/10.1016/J.CHOM.2021.02.003spa
dc.relation.referencesHadfield, J., Megill, C., Bell, S. M., Huddleston, J., Potter, B., Callender, C., Sagulenko, P., Bedford, T., & Neher, R. A. (2018). NextStrain: Real-time tracking of pathogen evolution. Bioinformatics, 34(23), 4121–4123. https://doi.org/10.1093/BIOINFORMATICS/BTY407spa
dc.relation.referencesHarrison, S. C. (2008). Viral membrane fusion. Nature Structural & Molecular Biology, 15(7), 690. https://doi.org/10.1038/NSMB.1456spa
dc.relation.referencesHernández, M., García-Morán, E., Abad, D., & Eiros, J. M. (n.d.). GISAID: INICIATIVA INTERNACIONAL PARA COMPARTIR DATOS GENÓMICOS DEL VIRUS DE LA GRIPE Y DEL SARS-CoV-2. Retrieved December 3, 2023, from www.mscbs.es/respspa
dc.relation.referencesHeurich, A., Hofmann-Winkler, H., Gierer, S., Liepold, T., Jahn, O., & Pöhlmann, S. (2014). TMPRSS2 and ADAM17 Cleave ACE2 Differentially and Only Proteolysis by TMPRSS2 Augments Entry Driven by the Severe Acute Respiratory Syndrome Coronavirus Spike Protein. Journal of Virology, 88(2), 1293–1307. https://doi.org/10.1128/JVI.02202-13spa
dc.relation.referencesHirotsu, Y., & Omata, M. (2021). Discovery of a SARS-CoV-2 variant from the P.1 lineage harboring K417T/E484K/N501Y mutations in Kofu, Japan. The Journal of Infection, 82(6), 276. https://doi.org/10.1016/J.JINF.2021.03.013spa
dc.relation.referencesHoffmann, M., Kleine-Weber, H., Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A., Müller, M. A., Drosten, C., & Pöhlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 181(2), 271-280.e8. https://doi.org/10.1016/j.cell.2020.02.052spa
dc.relation.referencesHolmes, E. C., Goldstein, S. A., Rasmussen, A. L., Robertson, D. L., Crits-Christoph, A., Wertheim, J. O., Anthony, S. J., Barclay, W. S., Boni, M. F., Doherty, P. C., Farrar, J., Geoghegan, J. L., Jiang, X., Leibowitz, J. L., Neil, S. J. D., Skern, T., Weiss, S. R., Worobey, M., Andersen, K. G., … Rambaut, A. (n.d.). Leading Edge The origins of SARS-CoV-2: A critical review. https://doi.org/10.1016/j.cell.2021.08.017spa
dc.relation.referencesHu, B., Guo, H., Zhou, P., & Shi, Z.-L. (n.d.). Characteristics of SARS-CoV-2 and COVID-19. https://doi.org/10.1038/s41579-020-00459-7spa
dc.relation.referencesHwang, S. S., Lim, J., Yu, Z., Kong, P., Sefik, E., Xu, H., Harman, C. C. D., Kim, L. K., Lee, G. R., Li, H. B., & Flavell, R. A. (2020). Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science (New York, N.y.), 367(6483), 1260. https://doi.org/10.1126/SCIENCE.ABB2507spa
dc.relation.referencesInstituto Nacional de Salud. (n.d.). Coronavirus Colombia. Coronavirus Colombia. Retrieved January 26, 2024, from https://www.ins.gov.co/Noticias/Paginas/Coronavirus.aspxspa
dc.relation.referencesInstituto Nacional de Salud | Colombia Documentos tecnicos genomica. (n.d.). Retrieved January 23, 2024, from https://www.ins.gov.co/Paginas/Documentos-tecnicos-genomica.aspxspa
dc.relation.referencesKorber, B., Fischer, W. M., Gnanakaran, S., Yoon, H., Theiler, J., Abfalterer, W., Hengartner, N., Giorgi, E. E., Bhattacharya, T., Foley, B., Hastie, K. M., Parker, M. D., Partridge, D. G., Evans, C. M., Freeman, T. M., de Silva, T. I., Angyal, A., Brown, R. L., Carrilero, L., … Montefiori, D. C. (2020). Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus. Cell, 182(4), 812. https://doi.org/10.1016/J.CELL.2020.06.043spa
dc.relation.referencesKuja, J. O., Kanoi, B. N., Balboa, R. F., Shiluli, C., Maina, M., Waweru, H., Gathii, K., Mungai, M., Masika, M., Anzala, O., Mwau, M., Clark, T. G., Waitumbi, J., & Gitaka, J. (2022). Genomic surveillance of SARS-COV-2 reveals diverse circulating variant lineages in Nairobi and Kiambu Counties, Kenya. BMC Genomics, 23(1). https://doi.org/10.1186/S12864-022-08853-6spa
dc.relation.referencesLaiton-Donato, K., Franco-Muñoz, C., Álvarez-Díaz, D. A., Ruiz-Moreno, H. A., Usme-Ciro, J. A., Prada, D. A., Reales-González, J., Corchuelo, S., Herrera-Sepúlveda, M. T., Naizaque, J., Santamaría, G., Rivera, J., Rojas, P., Ortiz, J. H., Cardona, A., Malo, D., Prieto-Alvarado, F., Gómez, F. R., Wiesner, M., … Mercado-Reyes, M. (2021). Characterization of the emerging B.1.621 variant of interest of SARS-CoV-2. Infection, Genetics and Evolution, 95, 105038. https://doi.org/10.1016/J.MEEGID.2021.105038spa
dc.relation.referencesLaiton-Donato, K., Usme-Ciro, J. A., Franco-Muñoz, C., Álvarez-Díaz, D. A., Ruiz-Moreno, H. A., Reales-González, J., Prada, D. A., Corchuelo, S., Herrera-Sepúlveda, M. T., Naizaque, J., Santamaría, G., Wiesner, M., Walteros, D. M., Ospina Martínez, M. L., & Mercado-Reyes, M. (2021a). Novel Highly Divergent SARS-CoV-2 Lineage With the Spike Substitutions L249S and E484K. Frontiers in Medicine, 8, 697605. https://doi.org/10.3389/FMED.2021.697605/FULLspa
dc.relation.referencesLaiton-Donato, K., Usme-Ciro, J. A., Franco-Muñoz, C., Álvarez-Díaz, D. A., Ruiz-Moreno, H. A., Reales-González, J., Prada, D. A., Corchuelo, S., Herrera-Sepúlveda, M. T., Naizaque, J., Santamaría, G., Wiesner, M., Walteros, D. M., Ospina Martínez, M. L., & Mercado-Reyes, M. (2021b). Novel Highly Divergent SARS-CoV-2 Lineage With the Spike Substitutions L249S and E484K. Frontiers in Medicine, 8, 697605. https://doi.org/10.3389/FMED.2021.697605/FULLspa
dc.relation.referencesLan, J., Ge, J., Yu, J., Shan, S., Zhou, H., Fan, S., Zhang, Q., Shi, X., Wang, Q., Zhang, L., & Wang, X. (2020). Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature, 581(7807), 215–220. https://doi.org/10.1038/S41586-020-2180-5spa
dc.relation.referencesLi, Q., Guan, X., Wu, P., Wang, X., Zhou, L., Tong, Y., Ren, R., Leung, K. S. M., Lau, E. H. Y., Wong, J. Y., Xing, X., Xiang, N., Wu, Y., Li, C., Chen, Q., Li, D., Liu, T., Zhao, J., Liu, M., … Feng, Z. (2020). Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia. The New England Journal of Medicine, 382(13), 1199. https://doi.org/10.1056/NEJMOA2001316spa
dc.relation.referencesLieb, W., Graf, J., Götz, A., König, I. R., Mayer, B., Fischer, M., Stritzke, J., Hengstenberg, C., Holmer, S. R., Döring, A., Löwel, H., Schunkert, H., & Erdmann, J. (2006). Association of angiotensin-converting enzyme 2 (ACE2) gene polymorphisms with parameters of left ventricular hypertrophy in men. Results of the MONICA Augsburg echocardiographic substudy. Journal of Molecular Medicine (Berlin, Germany), 84(1), 88–96. https://doi.org/10.1007/S00109-005-0718-5spa
dc.relation.referencesLorenzo-Redondo, R., Nam, H. H., Roberts, S. C., Simons, L. M., Jennings, L. J., Qi, C., Achenbach, C. J., Hauser, A. R., Ison, M. G., Hultquist, J. F., & Ozer, E. A. (n.d.). A Unique Clade of SARS-CoV-2 Viruses is Associated with Lower Viral Loads in Patient Upper Airways. https://doi.org/10.1101/2020.05.19.20107144spa
dc.relation.referencesLu, R., Zhao, X., Li, J., Niu, P., Yang, B., Wu, H., Wang, W., Song, H., Huang, B., Zhu, N., Bi, Y., Ma, X., Zhan, F., Wang, L., Hu, T., Zhou, H., Hu, Z., Zhou, W., Zhao, L., … Tan, W. (2020). Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Www.Thelancet.Com, 395, 565. https://doi.org/10.1016/S0140-6736(20)30251-8spa
dc.relation.referencesMartínez-Gómez, L. E., Herrera-López, B., Martinez-Armenta, C., Ortega-Peña, S., Camacho-Rea, M. del C., Suarez-Ahedo, C., Vázquez-Cárdenas, P., Vargas-Alarcón, G., Rojas-Velasco, G., Fragoso, J. M., Vidal-Vázquez, P., Ramírez-Hinojosa, J. P., Rodríguez-Sánchez, Y., Barrón-Díaz, D., Moreno, M. L., Martínez-Ruiz, F. de J., Zayago-Angeles, D. M., Mata-Miranda, M. M., Vázquez-Zapién, G. J., López-Reyes, A. (2022). ACE and ACE2 Gene Variants Are Associated With Severe Outcomes of COVID-19 in Men. Frontiers in Immunology, 13, 1. https://doi.org/10.3389/FIMMU.2022.812940/FULLspa
dc.relation.referencesMedina-Enríquez, M. M., Lopez-León, S., Carlos-Escalante, J. A., Aponte-Torres, Z., Cuapio, A., & Wegman-Ostrosky, T. (2020). ACE2: the molecular doorway to SARS-CoV-2. In Cell and Bioscience (Vol. 10, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s13578-020-00519-8spa
dc.relation.referencesMenachery, V. D., Yount, B. L., Debbink, K., Agnihothram, S., Gralinski, L. E., Plante, J. A., Graham, R. L., Scobey, T., Ge, X. Y., Donaldson, E. F., Randell, S. H., Lanzavecchia, A., Marasco, W. A., Shi, Z. L., & Baric, R. S. (2015). A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature Medicine, 21(12), 1508–1513. https://doi.org/10.1038/nm.3985spa
dc.relation.referencesMio, C., Secco, C. D., Marzinotto, S., Pipan, C., Sozio, E., Tascini, C., Damante, G., & Curcio, F. (2022). Correspondence: Monitoring the SPREAD of the SARS-CoV-2 lineage B.1.621 in Udine, Italy. Journal of Clinical Pathology, 75(10), 712. https://doi.org/10.1136/JCLINPATH-2021-207810spa
dc.relation.referencesNextclade. (n.d.). Retrieved January 6, 2024, from https://clades.nextstrain.org/spa
dc.relation.referencesNieto-Torres, J. L., DeDiego, M. L., Verdiá-Báguena, C., Jimenez-Guardeño, J. M., Regla-Nava, J. A., Fernandez-Delgado, R., Castaño-Rodriguez, C., Alcaraz, A., Torres, J., Aguilella, V. M., & Enjuanes, L. (2014). Severe Acute Respiratory Syndrome Coronavirus Envelope Protein Ion Channel Activity Promotes Virus Fitness and Pathogenesis. PLoS Pathogens, 10(5). https://doi.org/10.1371/journal.ppat.1004077spa
dc.relation.referencesÖrd, M., Faustova, I., & Loog, M. (2020). The sequence at Spike S1/S2 site enables cleavage by furin and phospho-regulation in SARS-CoV2 but not in SARS-CoV1 or MERS-CoV. Scientific Reports 2020 10:1, 10(1), 1–10. https://doi.org/10.1038/s41598-020-74101-0spa
dc.relation.referencesOrganizaciòn mundial de la salud. (n.d.). Panel de control de la OMS sobre el coronavirus (COVID-19).spa
dc.relation.referencesOrganization, W. H. (2021). WHO-convened global study of origins of SARS-CoV-2: China part.spa
dc.relation.referencesPark, S. T., & Kim, J. (2016). Trends in Next-Generation Sequencing and a New Era for Whole Genome Sequencing. International Neurourology Journal, 20(Suppl 2), S76. https://doi.org/10.5213/INJ.1632742.371spa
dc.relation.referencesPekar, J. E., Magee, A., Parker, E., Moshiri, N., Izhikevich, K., Havens, J. L., Gangavarapu, K., Mariana, L., Serrano, M., Crits-Christoph, A., Matteson, N. L., Zeller, M., Levy, J. I., Wang, J. C., Hughes, S., Lee, J., Ching, K., Yan, Z., Tzer, R., … Wertheim, J. O. (n.d.). The molecular epidemiology of multiple zoonotic origins of SARS-CoV-2. https://www.science.orgspa
dc.relation.referencesPrimer3. (n.d.). Retrieved January 5, 2024, from https://primer3.org/spa
dc.relation.referencesPropuestas aprobadas | ICTV. (n.d.). Retrieved December 8, 2023, from https://ictv.global/files/proposals/approved?fid=4929spa
dc.relation.referencesQuintana-Murci, L. (2016). Genetic and epigenetic variation of human populations: An adaptive tale. Comptes Rendus Biologies, 339(7–8), 278–283. https://doi.org/10.1016/J.CRVI.2016.04.005spa
dc.relation.referencesRambaut, A., Holmes, E. C., OToole, Á., Hill, V., McCrone, J. T., Ruis, C., du Plessis, L., & Pybus, O. G. (n.d.). A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology. Nature Microbiology. https://doi.org/10.1038/s41564-020-0770-5spa
dc.relation.referencesRavi, V., Saxena, S., & Panda, P. S. (2022). Basic virology of SARS-CoV 2. Indian Journal of Medical Microbiology, 40(2), 182. https://doi.org/10.1016/J.IJMMB.2022.02.005spa
dc.relation.referencesRivero, R., Garay, E., Botero, Y., Serrano-Coll, H., Gastelbondo, B., Muñoz, M., Ballesteros, N., Castañeda, S., Patiño, L. H., Ramirez, J. D., Calderon, A., Guzmán, C., Martinez-Bravo, C., Aleman, A., Arrieta, G., & Mattar, S. (2022). Human-to-dog transmission of SARS-CoV-2, Colombia. Scientific Reports, 12(1). https://doi.org/10.1038/S41598-022-11847-9spa
dc.relation.referencesSabater Molina, M., Nicolás Rocamora, E., Bendicho, A. I., Vázquez, E. G., Zorio, E., Rodriguez, F. D., Gil Ortuño, C., Rodríguez, A. I., Sánchez-López, A. J., Jara Rubio, R., Moreno-Docón, A., Marcos, P. J., García Pavía, P., Villa, R. B., & Gimeno Blanes, J. R. (2022). Polymorphisms in ACE, ACE2, AGTR1 genes and severity of COVID-19 disease. PloS One, 17(2), e0263140. https://doi.org/10.1371/JOURNAL.PONE.0263140spa
dc.relation.referencesSahajpal, V., Rajput, S., Sharma, T., Sharma, A., & Thakar, M. K. (2019). Development and evaluation of a novel DNA purification buffer and protocol for blood samples on FTA cards. Forensic Science International: Reports, 1, 100014. https://doi.org/10.1016/J.FSIR.2019.100014spa
dc.relation.referencesSecuenciación genómica y caracterización genética del virus de la influenza | CDC. (n.d.). Retrieved January 27, 2024, from https://espanol.cdc.gov/flu/about/professionals/genetic-characterization.htmspa
dc.relation.referencesSecuenciacion-genoma-completo-SARS-COV-2-secuenciador-MINION. (n.d.).spa
dc.relation.referencesSenapati, S., Banerjee, P., Bhagavatula, S., Kushwaha, P. P., & Kumar, S. (2021). Contributions of human ACE2 and TMPRSS2 in determining host–pathogen interaction of COVID-19. In Journal of Genetics (Vol. 100, Issue 1). Springer. https://doi.org/10.1007/s12041-021-01262-wspa
dc.relation.referencesShang, J., Ye, G., Shi, K., Wan, Y., Luo, C., Aihara, H., Geng, Q., Auerbach, A., & Li, F. (2020a). Structural basis of receptor recognition by SARS-CoV-2. Nature 2020 581:7807, 581(7807), 221–224. https://doi.org/10.1038/s41586-020-2179-yspa
dc.relation.referencesShang, J., Ye, G., Shi, K., Wan, Y., Luo, C., Aihara, H., Geng, Q., Auerbach, A., & Li, F. (2020b). Structural basis of receptor recognition by SARS-CoV-2. Nature, 581(7807), 221. https://doi.org/10.1038/S41586-020-2179-Yspa
dc.relation.referencesShovlin, C. L., & Vizcaychipi, M. P. (2020). COVID-19 genomic susceptibility: Definition of ACE2 variants relevant to human infection with SARS-CoV-2 in the context of ACMG/AMP Guidance. MedRxiv, 2020.05.12.20098160. https://doi.org/10.1101/2020.05.12.20098160spa
dc.relation.referencesSiddell, S. G., Walker, P. J., Lefkowitz, E. J., Mushegian, A. R., Adams, M. J., Dutilh, B. E., Gorbalenya, A. E., Harrach, B., Harrison, R. L., Junglen, S., Knowles, N. J., Kropinski, A. M., Krupovic, M., Kuhn, J. H., Nibert, M., Rubino, L., Sabanadzovic, S., Sanfaçon, H., Simmonds, P., … Davison, A. J. (2019). Additional changes to taxonomy ratified in a special vote by the International Committee on Taxonomy of Viruses (October 2018). Archives of Virology, 164(3), 943–946. https://doi.org/10.1007/S00705-018-04136-2spa
dc.relation.referencesSimmons, G., Gosalia, D. N., Rennekamp, A. J., Reeves, J. D., Diamond, S. L., & Bates, P. (2005). Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry. Proceedings of the National Academy of Sciences of the United States of America, 102(33), 11876. https://doi.org/10.1073/PNAS.0505577102spa
dc.relation.referencesSimmons, G., Zmora, P., Gierer, S., Heurich, A., & Pöhlmann, S. (2013). Proteolytic activation of the SARS-coronavirus spike protein: Cutting enzymes at the cutting edge of antiviral research. Antiviral Research, 100(3), 605. https://doi.org/10.1016/J.ANTIVIRAL.2013.09.028spa
dc.relation.referencesSingh, D., & Yi, S. V. (2021). On the origin and evolution of SARS-CoV-2. Experimental & Molecular Medicine, 53, 537–547. https://doi.org/10.1038/s12276-021-00604-zspa
dc.relation.referencesSituación del SARS CoV2 - Región de las Américas - OPS/OMS | Organización Panamericana de la Salud. (n.d.). Retrieved January 13, 2024, from https://www.paho.org/en/covid-19-weekly-updates-region-americasspa
dc.relation.referencesSun, P., Lu, X., Xu, C., Sun, W., & Pan, B. (2020). Understanding of COVID-19 based on current evidence. Journal of Medical Virology, 92(6), 548–551. https://doi.org/10.1002/JMV.25722spa
dc.relation.referencesTegally, H., Wilkinson, E., Giovanetti, M., Iranzadeh, A., Fonseca, V., Giandhari, J., Doolabh, D., Pillay, S., San, E. J., Msomi, N., Mlisana, K., von Gottberg, A., Walaza, S., Allam, M., Ismail, A., Mohale, T., Glass, A. J., Engelbrecht, S., Van Zyl, G., … de Oliveira, T. (2021). Detection of a SARS-CoV-2 variant of concern in South Africa. Nature, 592(7854), 438–443. https://doi.org/10.1038/S41586-021-03402-9spa
dc.relation.referencesThe COVID-19 Host Genetics Initiative, a global initiative to elucidate the role of host genetic factors in susceptibility and severity of the SARS-CoV-2 virus pandemic. (2020). European Journal of Human Genetics, 28(6), 715–718. https://doi.org/10.1038/s41431-020-0636-6spa
dc.relation.referencesThomas, S. (2021). Mapping the Nonstructural Transmembrane Proteins of Severe Acute Respiratory Syndrome Coronavirus 2. Journal of Computational Biology, 28(9), 909–921. https://doi.org/10.1089/cmb.2020.0627spa
dc.relation.referencesTorre-Fuentes, L., Matías-Guiu, J., Hernández-Lorenzo, L., Montero-Escribano, P., Pytel, V., Porta-Etessam, J., Gómez-Pinedo, U., & Matías-Guiu, J. A. (2021). ACE2, TMPRSS2, and Furin variants and SARS-CoV-2 infection in Madrid, Spain. Journal of Medical Virology, 93(2), 863–869. https://doi.org/10.1002/jmv.26319spa
dc.relation.referencesTortorici, M. A., & Veesler, D. (2019). Structural insights into coronavirus entry. In Advances in Virus Research (Vol. 105, pp. 93–116). Academic Press Inc. https://doi.org/10.1016/bs.aivir.2019.08.002spa
dc.relation.references(US), N. I. of H., & Study, B. S. C. (2007). Understanding Human Genetic Variation. https://www.ncbi.nlm.nih.gov/books/NBK20363/spa
dc.relation.referencesVadgama, N., Kreymerman, A., Campbell, J., Shamardina, O., Brugger, C., Research Consortium, G. E., Deaconescu, A. M., Lee, R. T., Penkett, C. J., Gifford, C. A., Mercola, M., Nasir, J., & Karakikes, I. (2022). SARS-CoV-2 Susceptibility and ACE2 Gene Variations Within Diverse Ethnic Backgrounds. Frontiers in Genetics, 13, 888025. https://doi.org/10.3389/FGENE.2022.888025/BIBTEXspa
dc.relation.referencesVolz, E., Mishra, S., Chand, M., Barrett, J. C., Johnson, R., Geidelberg, L., Hinsley, W. R., Laydon, D. J., Dabrera, G., O’Toole, Á., Amato, R., Ragonnet-Cronin, M., Harrison, I., Jackson, B., Ariani, C. V., Boyd, O., Loman, N. J., McCrone, J. T., Gonçalves, S., … Ferguson, N. M. (2021). Assessing transmissibility of SARS-CoV-2 lineage B.1.1.7 in England. Nature, 593(7858), 266–269. https://doi.org/10.1038/S41586-021-03470-Xspa
dc.relation.referencesWalls, A. C., Park, Y. J., Tortorici, M. A., Wall, A., McGuire, A. T., & Veesler, D. (2020). Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell, 181(2), 281. https://doi.org/10.1016/J.CELL.2020.02.058spa
dc.relation.referencesWang, N., Li, S. Y., Yang, X. Lou, Huang, H. M., Zhang, Y. J., Guo, H., Luo, C. M., Miller, M., Zhu, G., Chmura, A. A., Hagan, E., Zhou, J. H., Zhang, Y. Z., Wang, L. F., Daszak, P., & Shi, Z. L. (2018). Serological Evidence of Bat SARS-Related Coronavirus Infection in Humans, China. Virologica Sinica, 33(1), 104. https://doi.org/10.1007/S12250-018-0012-7spa
dc.relation.referencesWHO COVID-19 weekly epidemiological update vol. 74... - Google Académico. (n.d.). Retrieved January 2, 2024, from https://scholar.google.com/scholar?q=WHO+COVID-19+weekly+epidemiological+update+vol.+74+January+2022+World+Health+organization+spa
dc.relation.referencesWolf, J. M., Wolf, L. M., Bello, G. L., Maccari, J. G., & Nasi, L. A. (2023). Molecular evolution of SARS‐CoV‐2 from December 2019 to August 2022. Journal of Medical Virology, 95(1). https://doi.org/10.1002/JMV.28366spa
dc.relation.referencesWong, S. K., Li, W., Moore, M. J., Choe, H., & Farzan, M. (2004). A 193-Amino Acid Fragment of the SARS Coronavirus S Protein Efficiently Binds Angiotensin-converting Enzyme 2. Journal of Biological Chemistry, 279(5), 3197–3201. https://doi.org/10.1074/jbc.C300520200spa
dc.relation.referencesWu, C. rong, Yin, W. chao, Jiang, Y., & Xu, H. E. (2022). Structure genomics of SARS-CoV-2 and its Omicron variant: drug design templates for COVID-19. In Acta Pharmacologica Sinica (Vol. 43, Issue 12, pp. 3021–3033). Springer Nature. https://doi.org/10.1038/s41401-021-00851-wspa
dc.relation.referencesWu, F., Zhao, S., Yu, B., Chen, Y.-M., Wang, W., Song, Z.-G., Hu, Y., Tao, Z.-W., Tian, J.-H., Pei, Y.-Y., Yuan, M.-L., Zhang, Y.-L., Dai, F.-H., Liu, Y., Wang, Q.-M., Zheng, J.-J., Xu, L., Holmes, E. C., & Zhang, Y.-Z. (2008). A new coronavirus associated with human respiratory disease in China. Nature, 579. https://doi.org/10.1038/s41586-020-2008-3spa
dc.relation.referencesXiao, X., Newman, C., Buesching, C. D., Macdonald, D. W., & Zhou, Z.-M. (123 C.E.). Animal sales from Wuhan wet markets immediately prior to the COVID-19 pandemic. Scientific Reports |, 11, 11898. https://doi.org/10.1038/s41598-021-91470-2spa
dc.relation.referencesXie, X., Liu, Y., Liu, J., Zhang, X., Zou, J., Fontes-Garfias, C. R., Xia, H., Swanson, K. A., Cutler, M., Cooper, D., Menachery, V. D., Weaver, S. C., Dormitzer, P. R., & Shi, P. Y. (2021). Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K and N501Y variants by BNT162b2 vaccine-elicited sera. Nature Medicine, 27(4), 620–621. https://doi.org/10.1038/S41591-021-01270-4spa
dc.relation.referencesYadav, R., Chaudhary, J. K., Jain, N., Chaudhary, P. K., Khanra, S., Dhamija, P., Sharma, A., Kumar, A., & Handu, S. (2021). cells Role of Structural and Non-Structural Proteins and Therapeutic Targets of SARS-CoV-2 for COVID-19. 10, 821. https://doi.org/10.3390/cellsspa
dc.relation.referencesYan, R., Zhang, Y., Li, Y., Xia, L., Guo, Y., & Zhou, Q. (2020). Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. In Science (Vol. 367).spa
dc.relation.referencesYang, J., Petitjean, S. J. L., Koehler, M., Zhang, Q., Dumitru, A. C., Chen, W., Derclaye, S., Vincent, S. P., Soumillion, P., & Alsteens, D. (2020). Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-18319-6spa
dc.relation.referencesYoshimoto, F. K. (1234). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal, 39, 198–216. https://doi.org/10.1007/s10930-020-09901-4spa
dc.relation.referencesZeberg, H., & Pääbo, S. (2020). The major genetic risk factor for severe COVID-19 is inherited from Neanderthals. Nature 2020 587:7835, 587(7835), 610–612. https://doi.org/10.1038/s41586-020-2818-3spa
dc.relation.referencesZhu, L., Marsh, J. W., Griffith, M. P., Collins, K., Srinivasa, V., Waggle, K., Van Tyne, D., Snyder, G. M., Phan, T., Wells, A., Marroquin, O. C., & Harrison, L. H. (2022). Predictive model for severe COVID-19 using SARS-CoV-2 whole-genome sequencing and electronic health record data, March 2020-May 2021. PLoS ONE, 17(7). https://doi.org/10.1371/JOURNAL.PONE.0271381spa
dc.relation.referencesZhu, N., Zhang, D., Wang, W., Li, X., Yang, B., Song, J., Zhao, X., Huang, B., Shi, W., Lu, R., Niu, P., Zhan, F., Ma, X., Wang, D., Xu, W., Wu, G., Gao, G. F., Tan, W., & China Novel Coronavirus Investigating and Research Team. (2020). A Novel Coronavirus from Patients with Pneumonia in China, 2019. The New England Journal of Medicine, 382(8), 727–733. https://doi.org/10.1056/NEJMoa2001017spa
dc.relation.referencesGlowacka, I., Bertram, S., Müller, M. A., Allen, P., Soilleux, E., Pfefferle, S., Steffen, I., Tsegaye, T. S., He, Y., Gnirss, K., Niemeyer, D., Schneider, H., Drosten, C., & Pöhlmann, S. (2011b). Evidence that TMPRSS2 Activates the Severe Acute Respiratory Syndrome Coronavirus Spike Protein for Membrane Fusion and Reduces Viral Control by the Humoral Immune Response. Journal of Virology, 85(9), 4122–4134. https://doi.org/10.1128/JVI.02232-10spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.subject.bneCovid-19
dc.subject.bneSARS-CoV-2
dc.subject.ddc570 - Biología::576 - Genética y evoluciónspa
dc.subject.proposalReceptor ACE2 humanospa
dc.subject.proposalregión Spikespa
dc.subject.proposalvariantespa
dc.subject.proposallinaje viralspa
dc.subject.proposalHuman ACE2 receptoreng
dc.subject.proposalSpike regióneng
dc.subject.proposalvarianteng
dc.subject.proposalviral lineageeng
dc.subject.wikidataACE2
dc.subject.wikidataEnzima convertidora de angiotensina
dc.subject.wikidataSpike
dc.titleAnálisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19spa
dc.title.translatedAnalysis of genetic variability in seven exons of the human ACE2 gene and its relationship with the SARS-CoV-2 lineage in a group of individuals diagnosed with COVID-19eng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentBibliotecariosspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentPersonal de apoyo escolarspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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