Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)

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dc.contributor.advisorBarreto Hernández, Emiliano
dc.contributor.advisorReguero Reza, María Teresa Jesús
dc.contributor.authorCuello Mejia, Mishelle
dc.contributor.researchgroupGrupo de Bioinformáticaspa
dc.contributor.researchgroupGrupo de Epidemiología Molecularspa
dc.date.accessioned2023-07-07T13:55:45Z
dc.date.available2023-07-07T13:55:45Z
dc.date.issued2022-10-07
dc.descriptionilustraciones, fotografías, mapasspa
dc.description.abstractPseudomonas aeruginosa es uno de los principales microorganismos causantes de Infecciones Asociadas a la Atención en Salud, importante en el contexto epidemiológico actual debido a la emergencia de resistencia a los carbapenémicos. Teniendo en cuenta este planteamiento, el objetivo general del estudio consistió en realizar el seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de P. aeruginosa mediante secuenciación de genoma completo (WGS). Para ello, se planteó un estudio prospectivo, descriptivo en una institución hospitalaria de tercer nivel de complejidad en Bogotá-Colombia, durante los años 2019 y 2020; se analizaron aislamientos clínicos de P. aeruginosa provenientes de pacientes internados en los servicios de UCI y hospitalización. Se obtuvieron 43 aislamientos provenientes de 32 pacientes, secuenciados utilizando las plataformas Illumina y Oxford Nanopore. En cuanto a los genomas analizados, se anotaron 95 genes diferentes mediadores de resistencia, encontrándose la presencia del gen blaKPC-3, este es el segundo reporte de blaKPC-3 en P. aeruginosa en Colombia. Se detectó la presencia 14 secuencio-tipos (ST), siendo el ST111 el más frecuente, adicionalmente se identificaron 7 nuevos ST que a la fecha no han sido reportados. A partir de un árbol de SNP (Single Nucleotide Polymorphism), se evidenció la presencia de 3 grupos clonales. En conclusión, la investigación epidemiológica y genómica combinada permitió identificar las variaciones intrapaciente a nivel de perfiles genómicos de resistencia y la diversidad genética de los aislamientos de P. aeruginosa, lo cual posibilitó establecer posibles rutas de transmisión entre pacientes vinculados por proximidad en periodos de tiempo y espacio. (Texto tomado de la fuente)spa
dc.description.abstractPseudomonas aeruginosa is one of the main microorganisms that cause healthcare-associated infections (HAIs), important in the current epidemiological context due to the emergence of resistance to carbapenems. Taking this into consideration, the general objective of the study was to perform the epidemiological tracing of the genomic profiles of antibiotic resistance in clinical isolates of P. aeruginosa using Whole Genome Sequencing (WGS). For this, a prospective and descriptive study was proposed in a third level hospital in Bogotá-Colombia, during 2019 and 2020; clinical isolates of P. aeruginosa from patients of ICU and hospitalization services were analyzed. 43 isolates from 32 patients were obtained and sequenced using the Illumina and Oxford Nanopore platforms. Regarding the analyzed genomes, 95 different resistance mediator genes were annotated, finding the blaKPC-3 gene which is the second report of blaKPC-3 in P. aeruginosa in Colombia. The presence of 14 sequence-types (ST) were identified, being the ST111 the most frequent; in addition, 7 new STs that have not been previously reported were identified in this study. From a SNP (Single Nucleotide Polymorphism) tree, the presence of 3 clonal groups were evidenced. In conclusion, the epidemiological and genomic research allowed to identify intra-patient variations of the genomic resistance profiles and the genetic diversity of the P. aeruginosa isolates, leading to establish possible routes of transmission between patients linked by proximity in periods of time and space.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Microbiologíaspa
dc.description.researchareaBiología molecular de agentes infecciososspa
dc.format.extent124 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84156
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá,Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc610 - Medicina y saludspa
dc.subject.ddc000 - Ciencias de la computación, información y obras generalesspa
dc.subject.decsBacteriasspa
dc.subject.decsBacteriaeng
dc.subject.decsMonitoreo epidemiológicoepa
dc.subject.decsEpidemiological Monitoringeng
dc.subject.proposalPseudomonas aeruginosaspa
dc.subject.proposalInfecciones Asociadas a la Atención en Saludspa
dc.subject.proposalResistencia a antibióticosspa
dc.subject.proposalSeguimiento epidemiológicospa
dc.subject.proposalSecuenciación de Genoma Completospa
dc.subject.proposalGrupos clonalesspa
dc.subject.proposalSecuencio-tipos (ST)spa
dc.subject.proposalHealthcare-Associated Infections (HAIs)eng
dc.subject.proposalAntibiotic resistanceeng
dc.subject.proposalEpidemiological tracingeng
dc.subject.proposalWhole Genome Sequencing (WGS)eng
dc.subject.proposalSequence-types (ST)eng
dc.subject.proposalClonal groupseng
dc.titleSeguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)spa
dc.title.translatedEpidemiological tracing of the genomic profiles of antibiotic resistance in clinical isolates of P. aeruginosa using Whole Genome Sequencing (WGS)eng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
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