Genómica de rizobacterias entomopatógenas de Tecia solanivora (Lepidóptera: Gelechiidae)

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dc.contributor.advisorde Brito Brandão, Pedro Filipespa
dc.contributor.advisorVanegas Guerrero, Javierspa
dc.contributor.authorBoyacá Vásquez, Vivian Johannaspa
dc.contributor.corporatenameUniversidad Nacional de Colombiaspa
dc.contributor.corporatenameUniversidad Antonio Nariñospa
dc.contributor.researchgroupGrupo de Estudios para la Remediación y Mitigación de Impactos Negativos al Ambiente - GERMINAspa
dc.date.accessioned2020-08-05T23:30:11Zspa
dc.date.available2020-08-05T23:30:11Zspa
dc.date.issued2019-04-21spa
dc.description.abstractLas rizobacterias entomopatógenas presentan factores de virulencia como la producción de compuestos antimicrobianos, enzimas degradadoras y toxinas que han sido poco explorados. Mediante genómica se han realizado acercamientos para entender la interacción entre rizobacterias e insectos plaga. El objetivo de este trabajo fue determinar los factores de virulencia de rizobacterias entomopatógenas de Tecia solanivora mediante un acercamiento genómico. Para esto, se caracterizó el genoma de dos rizobacterias entomopatógenas que causaron una mortalidad superior al 75% en T. solanivora, y se compararon los factores de virulencia con genomas reportados de rizobacterias entomopatógenas. Se realizó extracción de ADN genómico, secuenciación masiva utilizando HiSeq 4000 (Illumina), ensamblaje y anotación y se determinó el porcentaje de similitud. Las lecturas de Raoultella C47 y Enterobacter TN152 fueron ensambladas en 58 y 121 contigs, respectivamente, con un tamaño de genoma medio de 5,4 kb, sin presencia de plásmidos. Se encontró que las categorías más relevantes fueron metabolismo, procesamiento de proteínas y respuesta a estrés, defensa y virulencia. Raoultella C47 mostró alta diversidad de compuestos volátiles incluyendo el ácido cianhídrico y presentó un gen para ramnolípidos. Enterobacter TN152 presentó dos toxinas entomopatógenas homólogas a toxinas Tc. Se detectaron enzimas degradadoras en ambos genomas. Al comparar con otras 14 rizobacterias, se encontró un 55% de similitud, y el perfil entomopatógeno más cercano para Raoultella C47 y Enterobacter TN152 fue Yersinia entomophaga MH96. Este estudio contribuye a entender los mecanismos de patogenicidad de rizobacterias, extendiendo el limitado grupo de rizobacterias entomopatógenas que se conocen y su respectiva secuenciación.spa
dc.description.abstractEntomopathogenic rhizobacteria present virulence factors such as the production of antimicrobial compounds, degrading enzymes and toxins, which have been little explored. By genomic methods, approaches have been made to understand the interaction between rhizobacteria and pest insects. The objective of this work was to determine the virulence factors of entomopathogenic rhizobacteria of Tecia solanivora using a genomic approach. The genome of two entomopathogenic rhizobacteria causing a mortality greater than 75% on T. Solanivora was characterized and virulence factors were compared with reported genomes of entomopathogenic rhizobacteria. Genomic DNA was extracted and massive sequencing was performed using HiSeq 4000 (Illumina). Assembly and annotation and the percentage of similarity was determined. The Raoultella C47 and Enterobacter TN152 readings were assembled in 58 and 121 contigs, respectively, with an average genome size of 5.4 kb without the presence of plasmids. The most relevant categories were metabolism, protein processing and response to stress, defense and virulence. Raoultella C47 showed high diversity of volatile compounds including hydrocyanic acid; a gene for ramnolipids was also found. Enterobacter TN152 showed two entomopathogenic toxins homologous to Tc toxins. Degrading enzymes were detected in both genomes. When comparing against other 14 rhizobacteria, a similarity of 55% was found, and the closest entomopathogenic profile for Raoultella C47 and Enterobacter TN152 was Yersinia entomophaga MH96. This study helps to understand the mechanisms of pathogenicity of rhizobacteria, extending the limited group of entomopathogenic rhizobacteria that are known and their respective sequencing.spa
dc.description.additionalLínea de Investigación Micro biología Ambiental y Aplicada.spa
dc.description.degreelevelMaestríaspa
dc.description.projectInteracciones entre Tecia solanivora, rizobacterias con actividad entomopatógena y plantas de papa para favorecer la competitividad de la cadena papera en el Departamento de Boyacáspa
dc.description.sponsorshipCOLCIENCIAS, LA GOBERNACIÓN DE BOYACÁ, LA UNIVERSIDAD ANTONIO NARIÑOspa
dc.format.extent86spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationBoyacá-Vásquez V.spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/77948
dc.language.isospaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.departmentDepartamento de Químicaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Bioquímicaspa
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dc.rightsDerechos reservados - Universidad Nacional de Colombiaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.rights.spaAcceso abiertospa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.ddc630 - Agricultura y tecnologías relacionadasspa
dc.subject.ddc550 - Ciencias de la tierraspa
dc.subject.ddc540 - Química y ciencias afinesspa
dc.subject.proposalgenomicseng
dc.subject.proposalmassive sequencingeng
dc.subject.proposalrizobacteriasspa
dc.subject.proposalbiocontroleng
dc.subject.proposalentomopathogenic activityeng
dc.subject.proposalinsecto-plagaspa
dc.subject.proposaltecia solanivoraspa
dc.titleGenómica de rizobacterias entomopatógenas de Tecia solanivora (Lepidóptera: Gelechiidae)spa
dc.typeDocumento de trabajospa
dc.type.coarhttp://purl.org/coar/resource_type/c_8042spa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/workingPaperspa
dc.type.redcolhttp://purl.org/redcol/resource_type/WPspa
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