Estado actual de la investigación proteómica del protozoario patógeno Giardia lamblia

Abstract

Este trabajo presenta un panorama general de los estudios proteómicos realizados en Giardia lambía hasta el año 2016, junto con los resultados de una minería de datos preliminar realizada cruzando algunos de estos estudios proteómicos. El material presentado sirve de base para futuros estudios en proteómica y/o biología de sistemas y resalta aspectos interesantes que pueden ser profundizados posteriormente.

Abstract: Giardia lamblia is an intestinal parasite of medical and phylogenetic importance because it causes the gastrointestinal disease known as giardiasis and is also considered as a eukaryotic organism of early divergence. Proteomics is a post-genomic technology that makes use of various techniques such as uni and two-dimensional electrophoresis, high resolution chromatography, mass spectrometry, protein labelling techniques and the indisputable use of bioinformatics to identify the whole set of proteins that are present in a complete organism, part of it, or in a particular event. In Giardia, different proteomic investigations have been carried out to study aspects such as the encystment process, its morphology and cellular organelles (such as the mitosomes, vesicles and cytoskeleton), its virulence and resistance, and comparative studies between different strains of the parasite. This work presents an overview of the scope of such proteomic studies in Giardia. On the other hand, the results of a preliminary data mining performed through some of the revised proteomic studies are also shown. In this way, the material presented serves as a basis for the future realization of other studies in proteomics and / or systems biology and highlights interesting aspects that can be further studied.