Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia

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dc.contributor.advisorVargas Ramírez, Mario
dc.contributor.authorPolanco Rodríguez, María Camila
dc.contributor.subjectmatterexpertGómez Nieto, Diego
dc.coverage.countryColombia
dc.date.accessioned2024-02-01T16:09:27Z
dc.date.available2024-02-01T16:09:27Z
dc.date.issued2023
dc.descriptionilustraciones, diagramas, mapaspa
dc.description.abstractEl Cocodrilo del Orinoco (Crocodylus intermedius) es un reptil endémico de zonas bajas de la cuenca del río Orinoco. Actualmente la especie enfrenta serias amenazas para su supervivencia, por lo cual es necesario un plan de conservación integral que incluya programas modernos como el mantenimiento de un microbioma saludable, lo cual puede mejorar su mantenimiento, reproducción y liberación. Este estudio tuvo como objetivo caracterizar la microbiota bacteriana de las heces de C. intermedius, mantenidos en la mayor población ex-situ colombiana (EBTRF, Villavicencio, Meta) y comparar las poblaciones bacterianas entre cocodrilos juveniles y adultos. Se realizó la secuenciación del gen rRNA 16S de 24 muestras de materia fecal (Juveniles n=15; Adultos n=9), utilizando el equipo Illumina MiSeq (2×250 bp). Los resultados indican que la microbiota fecal de está predominada por Firmicutes, seguido por Bacteroidetes y Proteobacterias. Los índices de medición de Alfa diversidad y la estructura bacteriana no tuvieron una diferencia significativa entre los Juveniles y Adultos, sin embargo, el membership de las poblaciones bacterianas difirió significativamente entre los grupos. La composición de la microbiota fecal de ambos estuvo fuertemente predominada por Firmicutes y Bacteroidetes, distribución que se ha relacionado con enfermedades como la obesidad y desórdenes endocrinos. A pesar de que investigaciones previas han reportado bacterias patógenas en la microbiota fecal, los reptiles poseen un fuerte sistema inmunológico que podría conferir una mayor resistencia a infecciones, cáncer y senescencia. Esta investigación presenta una herramienta para la conservación del Cocodrilo del Orinoco y amplía el conocimiento de la microbiota en reptiles. (Texto tomado de la fuente)spa
dc.description.abstractThe Orinoco Crocodile (Crocodylus intermedius) is an endemic reptile found in lowland areas of the Orinoco River basin. Currently, the species faces serious threats to its survival, demanding an all-encompassing conservation program that includes modern programs such as maintaining a healthy microbiome, which can improve its maintenance, reproduction, and release. This study aimed to characterize the bacterial microbiota of feces from C. intermedius kept in the largest ex-situ Colombian population (EBTRF, Villavicencio, Meta) and compare the bacterial populations between juvenile and adult crocodiles. The 16S rRNA gene sequencing was performed on 24 fecal samples (Juveniles n=15; Adults n=9), using the Illumina MiSeq platform (2×250 bp). The results indicate that the fecal microbiota is predominantly composed of Firmicutes, followed by Bacteroidetes and Proteobacteria in both groups. Alpha diversity and bacterial structure measurements did not show significant differences between juveniles and adults; however, the membership of bacterial populations differed significantly between the groups. The majority presence of Firmicutes and Bacteroidetes have been associated with diseases such as obesity and endocrine disorders in reptiles. Despite previous reports of pathogenic bacteria in the fecal microbiota, reptiles possess a robust immune system that may confer greater resistance to infections, cancer, and senescence. This research provides a tool for the conservation of the Orinoco Crocodile and expands our understanding of reptilian fecal microbiota.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Microbiologíaspa
dc.format.extentxv, 62 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85587
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc570 - Biología::577 - Ecologíaspa
dc.subject.ddc590 - Animales::597 - Vertebrados de sangre fríaspa
dc.subject.lccFlora intestinalspa
dc.subject.lccIntestines-Microbiologyeng
dc.subject.lembFlora microbianaspa
dc.subject.lembMicrobial floraeng
dc.subject.lembCocodrilosspa
dc.subject.lembCrocodylidaeeng
dc.subject.proposalMicrobiotaspa
dc.subject.proposalCrocodileeng
dc.subject.proposalFeceseng
dc.subject.proposalGuteng
dc.subject.proposalCrocodylus intermediuseng
dc.subject.proposalCocodrilosspa
dc.subject.proposalHecesspa
dc.subject.proposalReptilesspa
dc.subject.wikidataMicrobiotaspa
dc.titleCaracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombiaspa
dc.title.translatedCharacterization of the gut bacterial microbiome of the critically endangered Orinoco Crocodile (Crocodylus intermedius), present in the largest ex situ population at the Roberto Franco Tropical Biology Station (EBTRF) in Villavicencio, Colombiaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
dcterms.audience.professionaldevelopmentMedios de comunicaciónspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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