Open-Source Label-Free Microscopy

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dc.contributor.advisorGarcia Sucerquia, Jorge Iván
dc.contributor.authorBuitrago Duque, Carlos Andrés
dc.contributor.cvlac0000127437spa
dc.contributor.googlescholarnCo6tr0AAAAJspa
dc.contributor.orcidBuitrago Duque, Carlos Andrés [0000-0001-7523-9735]spa
dc.contributor.researchgateCarlos-Buitrago-Duquespa
dc.contributor.researchgroupOptica y Procesamiento Opto-Digitalspa
dc.contributor.scopus56051519100spa
dc.date.accessioned2025-04-22T22:25:03Z
dc.date.available2025-04-22T22:25:03Z
dc.date.issued2024-10
dc.descriptionIlustraciones, fotografíasspa
dc.description.abstractThe rapid innovation in modern microscopy has outpaced its commercialization and adoption, leaving many users unable to access these advances. Even within the scientific community, adopting complex peer-reported designs can be challenging. To bridge the gap between microscopy innovation and widespread implementation, efforts are needed to democratize the field, making custom, research-grade instruments easily accessible to all. In this thesis, the development of open-source tools to perform label-free analysis in current science and engineering applications through digital holographic microscopy (DHM) systems, and its related technologies, was sought. To achieve it, simulation platforms, which account for the main parameters in an experimental setup, and numerical reconstruction tools that allow the digital processing of label-free recordings, were initially developed and openly distributed. Supported by these tools, accessible instrumentation of DHM systems was pursued, allowing the design and assembly of systems with increased portability and reduced cost and complexity. The developed devices and systems were validated in real-world applications, gathering valuable feedback that highlighted improvement opportunities. Finally, these opportunities were harnessed in the refinement of the proposed systems, both the software and hardware tools, enhancing their functionality. The results were reported on 13 manuscripts submitted to indexed journals of international circulation and shared in 17 presentations in international conferences. These works, which constitute the core of the present thesis, represent significant progress towards accessible, high-performance DHM and DLHM systems, with broad potential applications in both scientific research and education. (Tomado de la fuente)eng
dc.description.abstractLa rápida innovación en microscopía moderna ha superado su comercialización y adopción, dejando a muchos usuarios sin acceso a estos avances. Incluso dentro de la comunidad científica, la incorporación de diseños complejos reportados en la literatura es un desafío. Para cerrar la brecha entre la innovación en microscopía y su implementación generalizada, es necesario democratizar esta área de investigación, facilitando el acceso a instrumentos personalizados de nivel investigativo. En esta tesis se desarrollaron herramientas libres para realizar análisis sin marcadores en aplicaciones actuales de ciencia e ingeniería, mediante sistemas de microscopía holográfica digital (DHM) y tecnologías afines. Para ello, se desarrollaron y distribuyeron plataformas de simulación que incorporan los principales parámetros de un entorno experimental, y herramientas de reconstrucción numérica para el procesamiento digital de registros sin marcadores. Estas herramientas apoyaron el desarrollo de instrumentación accesible para sistemas DHM, permitiendo la creación de dispositivos más portátiles, económicos y de menor complejidad. Los sistemas desarrollados fueron validados en aplicaciones reales, recopilando retroalimentaciones y oportunidades de mejora, que luego fueron aprovechadas para optimizar tanto las herramientas de software como los dispositivos de hardware, mejorando su funcionalidad. Los resultados se reportaron en 13 manuscritos sometidos a revistas indexadas de circulación internacional y 17 presentaciones en conferencias internacionales. Estos trabajos, que constituyen el núcleo de esta tesis, representan un avance significativo hacia sistemas DHM y DLHM accesibles y de alto desempeño, con aplicaciones en investigación y docencia.spa
dc.description.curricularareaFísica.Sede Medellínspa
dc.description.degreelevelDoctoradospa
dc.description.degreenameDoctor en Ciencias - Físicaspa
dc.description.researchareaMicroscopía Holográfica Digitalspa
dc.format.extent98 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/88076
dc.language.isoengspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellínspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeMedellín, Colombiaspa
dc.publisher.programMedellín - Ciencias - Doctorado en Ciencias - Físicaspa
dc.relation.indexedLaReferenciaspa
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dc.subject.ddc530 - Física::535 - Luz y radiación relacionadaspa
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dc.subject.lembMicroscopía
dc.subject.lembHolografía
dc.subject.lembMicroscopía digital
dc.subject.lembNanotecnología
dc.subject.proposalDigital Holographic Microscopyeng
dc.subject.proposalOpen Microscopyeng
dc.subject.proposalLabel-Free Imagingeng
dc.subject.proposalQuantitative Phase Imagingeng
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dc.subject.proposalMicroscopía Holográfica Digitalspa
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dc.subject.proposalMicroscopía Abiertaspa
dc.subject.proposalImágenes sin marcadoresspa
dc.subject.proposalImágenes Cuantitativas de Fasespa
dc.titleOpen-Source Label-Free Microscopyeng
dc.title.translatedMicroscopía Abierta sin Marcadoresspa
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