Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular

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dc.contributor.advisorPerdomo Arciniegas, Ana María
dc.contributor.advisorFontanilla Duque, Martha Raquel
dc.contributor.authorUrrego Orrego, Karen Yurany
dc.contributor.orcidUrrego Orrego, Karen Yurany [000000023762826X]spa
dc.contributor.researchgroupGrupo de Investigación en Medicina transfusional, tisular y celular (GYMTIC)spa
dc.date.accessioned2024-06-28T14:37:21Z
dc.date.available2024-06-28T14:37:21Z
dc.date.issued2024
dc.descriptionilustraciones, diagramasspa
dc.description.abstractLa sangre de cordón umbilical (SCU) es una fuente de progenitores hematopoyéticos (PH) usados como terapia en diversas patologías, principalmente de tipo hematológico. En Colombia, el grupo de investigación en medicina transfusional, tisular y celular (GIMTTYC) del Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS), desarrolló un programa clínico de captación de donantes y de banqueo de células de SCU, así como servicios de búsqueda de compatibilidad, reserva y distribución de unidades, bajo estándares internacionales de terapia celular. Los procedimientos de congelación y descongelación de SCU se validan en cada banco con el fin de mantener viables las células que se usarán en el trasplante. La variación de las condiciones en estos procedimientos impacta la recuperación y viabilidad celular, que puede afectar la potencia terapéutica de los trasplantes. Con el fin de corroborar la funcionalidad de las células, antes y después de la descongelación de las unidades de SCU, se realizan pruebas de viabilidad celular por citometría de flujo y clonogenicidad. Por lo tanto, establecer las condiciones para mantener el número y la viabilidad celular después de la criopreservación, antes del trasplante es clave para garantizar la calidad de unidades en esta etapa. Para descongelar las unidades de SCU, estas se diluyen o lavan para disminuir el efecto citotóxico del crioprotector. Sin embargo, el GIMTTYC todavía requiere estandarizar el procedimiento de descongelación. En este trabajo se validó la implementación in situ de un protocolo pretrasplante de descongelación de unidades de SCU con la perspectiva de establecer herramientas que mejoren la recuperación y viabilidad celular pretrasplante. (Texto tomado de la fuente)spa
dc.description.abstractUmbilical cord blood (UCB) is a source of hematopoietic progenitors (HP), which are used as therapy in various pathologies, mainly hematological. In Colombia, the transfusion, tissue and cellular medicine research group (GIMTTYC) of the Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS), developed a clinical program for donor recruitment and banking of SCU cells, as well as services of compatibility search, reservation and distribution of units, under international standards of cellular therapy. SCU freezing and thawing procedures are validated in each bank in order to maintain the viability of the cells to be used in transplantation. Varying conditions in these procedures impact cell recovery and viability, which can affect the therapeutic potency of transplants. In order to corroborate that the cells will be functional, before and after thawing of the SCU units, cell viability tests are performed by flow cytometry and clonogenicity. Therefore, establishing the conditions to maintain cell number and viability after cryopreservation prior to transplantation is key to ensure the quality of units at this stage. To thaw SCU units prior to transplantation, they are diluted or washed to decrease the cytotoxic effect of the cryoprotectant. However, in the GIMTTYC this process still requires standardization. This work validated the in situ implementation of a pretransplantation protocol for thawing SCU units with the perspective of establishing tools to improve pretransplantation cell recovery and viability.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Bioquímicaspa
dc.description.researchareaInvestigación en fisiología y patología de la hematopoyesis para el desarrollo y la innovación clínicaspa
dc.format.extentxviii, 90 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/86328
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Bioquímicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc610 - Medicina y salud::612 - Fisiología humanaspa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.decsAcondicionamiento Pretrasplantespa
dc.subject.decsTransplantation Conditioningeng
dc.subject.decsBiotecnologíaspa
dc.subject.decsBiotechnologyeng
dc.subject.decsCongelaciónspa
dc.subject.decsFreezingeng
dc.subject.decsCordón Umbilicalspa
dc.subject.decsUmbilical Cordeng
dc.subject.proposalSangre de cordón umbilicalspa
dc.subject.proposalTrasplante de progenitores hematopoyéticosspa
dc.subject.proposalCriopreservaciónspa
dc.subject.proposalDiluciónspa
dc.subject.proposalLavadospa
dc.subject.proposalApoptosisspa
dc.subject.proposalNecrosisspa
dc.subject.proposalUmbilical cord bloodeng
dc.subject.proposalHematopoietic stem cell transplantationeng
dc.subject.proposalCryopreservationeng
dc.subject.proposalDilutioneng
dc.subject.proposalWashingeng
dc.subject.proposalApoptosiseng
dc.subject.proposalNecrosiseng
dc.titleOptimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celularspa
dc.title.translatedPretransplantation procedure optimization of umbilical cord blood units thawing: prevention of cell deatheng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
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dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleINVESTIGACIÓN ORIENTADA A LA IMPLEMENTACIÓN DE BUENAS PRÁCTICAS PARA LA APLICACIÓN CLÍNICA DE TERAPIAS CELULARES. MODELO: TPH EN BOGOTÁspa
oaire.fundernameFondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías, código BPIN2016000100035spa
oaire.fundernameFondo Financiero Distrital de Salud (Convenio 0182 de 2018)spa

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