Caracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing.

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dc.contributor.advisorMoreno Herrera, Claudia Ximena
dc.contributor.advisorArango Isaza, Rafael Eduardo
dc.contributor.authorPereira Bazurdo, Angie Natalia
dc.contributor.researchgroupMicrobiodiversidad y Bioprospecciónspa
dc.coverage.cityAntioquia, Colombia
dc.date.accessioned2021-10-12T13:09:30Z
dc.date.available2021-10-12T13:09:30Z
dc.date.issued2021
dc.descriptionilustraciones, tablasspa
dc.description.abstractEl control de plagas y enfermedades del aguacate depende en gran medida del uso de pesticidas. Nuevas alternativas de control, tales como el uso de sustancias bioactivas inhibidoras contra fitopatógenos producidas por microorganismos, brindan una opción viable para contrarrestar las pérdidas por plagas y enfermedades. La exploración de nuevas fuentes de microorganismos se ha ampliado a especies que no habían sido consideradas. El presente estudio tuvo como objetivo caracterizar la capacidad antagonista in vitro contra microorganismos fitopatógenos y la producción de moléculas de señalización ‘Quórum Sensing’ (QS) en cepas bacterianas aisladas de trips de aguacate. La actividad antagonista de diez aislados bacterianos fueron seleccionadas y evaluadas contra siete fitopatógenos (hongos, oomiceto y bacterias) por cultivo dual, difusión y ensayo en placa; las moléculas de señalización (QS) producidas por las cepas Gram negativas se detectaron a través de bioensayo con placas de cromatografía fina (TLC) y biosensores bacterianos específicos (psB401, psB1142 y pqsA-lux). Las pruebas de cultivo dual mostraron que cuatro cepas pertenecientes a los géneros Bacillus, Pantoea, y Serratia, antagonizaron la mayoría de los hongos y el oomiceto, limitando el normal crecimiento de las colonias y generando zonas de inhibición. Adicionalmente, a través de biosensores se detectaron moléculas AHL y AHQ como 3-Oxo-C12-HSL(OdDHL), 3-Oxo-C6-HSL (OHHL) y 2-heptil-4-quinolona (HHQ). Trazas de OdDHL y HHQ, y el hallazgo de dos ciclopéptidos Ciclo(L-Phe-L-Pro) y Ciclo(L-Pro-L-Tyr) por Cromatografía Líquida de Ultra Alto Rendimiento y Espectrometría de Masas (UHPLC/MS) en los extractos de las cepas Gram negativas, podrían explicar la actividad antifúngica detectada en las dos cepas de Pantoea sp., y Serratia sp., ya que estas moléculas se caracterizan por estar relacionadas con los sistemas de QS y poseen una variedad amplia de actividad biológica. Hasta donde sabemos, este sería uno de los primeros reportes sobre el potencial antagonista y la detección de moléculas de señalización QS en cepas aisladas de trips de aguacate. (Texto tomado de la fuente)spa
dc.description.abstractThe control of avocado pests and diseases depends largely on the use of various types of pesticides, most of which are either strictly monitored or not accepted internationally. New alternatives such as inhibitory bioactive substances against phytopathogens produced by microorganisms provide an excellent alternative for the biocontrol of pests and diseases, and the exploration of new sources of these microorganisms has been extended to organisms previously not considered. This study aims to characterize the antagonistic capacity in vitro against phytopathogenic microorganisms and the production of Quorum Sensing (QS) signaling molecules from bacterial strains isolated from avocado thrips. The antagonist activity of ten bacterial isolates of thrips was evaluated against seven phytopathogens (fungi, oomycetes, and bacteria) by dual culture, diffusion, and plate assay; signaling molecules (QS) produced by Gram negative strains were detected through bioassay with fine chromatography plates (TLC) and specific bacterial biosensors (psB401, psB1142 and pqsA-lux). Dual culture tests showed that four strains belonging to the genera Bacillus sp., Pantoea sp., and Serratia sp., antagonized most of the fungi and the oomycete, limiting the normal growth of the hyphae and generating zones of inhibition. Additionally, AHL and AHQ molecules such as 3-Oxo-C12-HSL (OdDHL), 3-Oxo-C6-HSL (OHHL), and 2-heptyl-4-quinolone (HHQ) were detected through biosensors. Traces of OdDHL and HHQ, and the finding of two cyclopeptides Cycle(L-Phe-L-Pro) and Cycle(L-Pro-L-Tyr) by Ultra-High-Performance Liquid Chromatography and Mass Spectrometry (UHPLC / MS) in the extracts of the Gram negative strains could explain the antifungal activity detected in the two strains of Pantoea sp., and Serratia sp., since these molecules are characterized by being related to QS systems and possess a variety of biological activities including antimicrobial. To our knowledge, this would be one of the first reports on the antagonistic potential and the detection of QS signaling molecules in isolated strains of avocado thrips.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Biotecnologíaspa
dc.description.researchareaBioprospecciónspa
dc.format.extent96 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/80505
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellínspa
dc.publisher.departmentEscuela de biocienciasspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeMedellín, Colombiaspa
dc.publisher.programMedellín - Ciencias - Maestría en Ciencias - Biotecnologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc580 - Plantasspa
dc.subject.ddc660 - Ingeniería químicaspa
dc.subject.lembAguacate - Enfermedades y plagas
dc.subject.lembBacteria, phytopathogenic
dc.subject.lembBacterias fitopatógenas
dc.subject.lembPatología vegetal
dc.subject.proposalActividad antifúngica,spa
dc.subject.proposalActividad antibacterianaspa
dc.subject.proposalAHLsspa
dc.subject.proposalDiketopiperazinasspa
dc.subject.proposalAntifungal activityeng
dc.subject.proposalAntibacterial activityeng
dc.subject.proposalDiketopiperazineseng
dc.titleCaracterización de cepas bacterianas obtenidas de trips (Thysanoptera: Thripidae) que afectan cultivos de aguacate (Persea americana Mill) en Antioquia: Actividad antagonista y Quorum Sensing.spa
dc.title.translatedCharacterization of bacterial strains obtained from thrips (Thysanoptera: Thripidae) that affect avocado crops (Persea americana Mill) in Antioquia: Antagonist activity and Quorum Sensing.fra
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
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dc.type.driverinfo:eu-repo/semantics/masterThesisspa
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dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.fundernameUniversidad Nacional de Colombiaspa
oaire.fundernameColcienciasspa

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