Expresión de Hand2 y Nphs2 en la morfogénesis renal del embrión de pollo

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dc.contributor.advisorAcosta Virgüez, Edwinspa
dc.contributor.advisorGaravito Aguilar, Zayra Vivianaspa
dc.contributor.authorTéllez Beltrán, Ana Isabelspa
dc.date.accessioned2025-04-01T19:53:30Z
dc.date.available2025-04-01T19:53:30Z
dc.date.issued2024
dc.descriptionilustraciones a color, fotografíasspa
dc.description.abstractEl riñón desarrolla un papel esencial en el mantenimiento de la homeostasis del individuo gracias a sus múltiples funciones reguladoras y excretoras. Las diversas patologías congénitas que lo afectan representan una causa importante de la enfermedad renal crónica en niños y adultos jóvenes; y en animales una causa común de glomerulopatías principalmente en perros y gatos jóvenes; por lo cual es esencial expandir los conocimientos a cerca de los genes que puedan desencadenar estas afecciones, tal como es el caso Hand2 y Nphs2 en un contexto regulador y funcional, respectivamente. Aunque se ha demostrado previamente la importancia que presenta el gen Nphs2 para el adecuado funcionamiento de los podocitos en la barrera de filtración renal y el papel esencial de Hand2 en términos de regulación morfológica del riñón, hasta el momento no se conoce su expresión en un contexto espacio temporal durante diferentes estadios del desarrollo embrionario del pollo. Por esto, el objetivo del presente estudio fue evaluar la expresión de ambos genes en un contexto espacio temporal de este modelo de investigación. Para cumplir con dicho objetivo se realizó evaluación de la expresión de ambos genes a través de RT-PCR, la cual se confirmó posteriormente con Hibridación in situ y además, en el caso de Nphs2 con inmunohistoquímica. Los resultados obtenidos fueron: La expresión de Nphs2 no es específica de los podocitos en el riñón, debido a que se detectó expresión en cabeza, corazón y extremidades, lo cual posiblemente signifique que su rol no es exclusivamente funcional de la barrera de filtración renal en el embrión de pollo. En el caso de Hand2 su expresión se detectó antes que Nphs2, lo cual refuerza su rol regulador y de diferenciación durante el desarrollo embrionario. Además, se expresó directamente en el metanefros, lo cual genera incógnitas acerca de su papel en el mesodermo intermedio y en el riñón del embrión de pollo (Texto tomado de la fuente).spa
dc.description.abstractThe kidney plays a crucial role in maintaining an individual's homeostasis through its multiple regulatory and excretory functions. Various congenital pathologies affecting it are a significant cause of chronic kidney disease in children and young adults and a common cause of glomerulopathies in young dogs and cats. Therefore, expanding knowledge about the genes that may trigger these conditions is essential, as is the case with Hand2 and Nphs2 in a regulatory and functional context, respectively. Although the Nphs2 gene has been previously shown to be essential for the proper functioning of podocytes in the renal filtration barrier and Hand2 plays a key role in the morphological regulation of the kidney, their spatial-temporal expression during different stages of chicken embryonic development remains unknown. Thus, the aim of this study was to evaluate the expression of both genes in a spatialtemporal context within this research model. To achieve this, gene expression was assessed through RT-PCR, later confirmed via in situ hybridization, and in the case of Nphs2, also through immunohistochemistry. The results showed that Nphs2 expression is not exclusive to podocytes in the kidney, as it was also detected in the head, heart, and limbs, suggesting that its role is not limited to renal filtration barrier function in the chicken embryo. Regarding Hand2, its expression was detected earlier than Nphs2, reinforcing its regulatory and differentiation role during embryonic development. Additionally, Hand2 was directly expressed in the metanephros, raising questions about its role in the intermediate mesoderm and the kidney of the chicken embryo.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Fisiologíaspa
dc.description.researchareaAnatomía y biología del desarrollospa
dc.format.extent84 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/87806
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Medicina - Maestría en Fisiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-CompartirIgual 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/spa
dc.subject.ddc610 - Medicina y salud::612 - Fisiología humanaspa
dc.subject.ddc610 - Medicina y salud::616 - Enfermedadesspa
dc.subject.decsMorfogénesisspa
dc.subject.decsMorphogenesiseng
dc.subject.decsFenómenos Fisiológicosspa
dc.subject.decsPhysiological Phenomenaeng
dc.subject.decsHomeostasisspa
dc.subject.decsHomeostasiseng
dc.subject.decsAnomalías Congénitasspa
dc.subject.decsCongenital Abnormalitieseng
dc.subject.decsInsuficiencia Renal Crónicaspa
dc.subject.decsRenal Insufficiency, Chroniceng
dc.subject.decsGlomerulonefritis Membranoproliferativaspa
dc.subject.decsGlomerulonephritis, Membranoproliferativeeng
dc.subject.decsDesarrollo Embrionariospa
dc.subject.decsEmbryonic Developmenteng
dc.subject.proposalMorfogénesis renalspa
dc.subject.proposalPatrón de expresiónspa
dc.subject.proposalPodocitosspa
dc.subject.proposalNphs2spa
dc.subject.proposalHand2spa
dc.subject.proposalRenal morphogenesiseng
dc.subject.proposalExpression patterneng
dc.subject.proposalPodocyteseng
dc.titleExpresión de Hand2 y Nphs2 en la morfogénesis renal del embrión de pollospa
dc.title.translatedExpression of Hand2 and Nphs2 in Renal Morphogenesis of the Chicken Embryoeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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