Comparison of dna extraction methods for detection of citrus huanglongbing in colombia

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dc.contributor.authorAngel, Jorge Eveliospa
dc.contributor.authorHernández, Erick Geovannispa
dc.contributor.authorHerrera, Néstor Andrésspa
dc.contributor.authorGómez, Linda Yhisetspa
dc.contributor.authorCastro, Ángela Patriciaspa
dc.contributor.authorSepúlveda, Adriana Milenaspa
dc.contributor.authorEbratt, Everth Emiliospa
dc.date.accessioned2019-07-03T16:39:09Zspa
dc.date.available2019-07-03T16:39:09Zspa
dc.date.issued2014-03-26spa
dc.description.abstractFour DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al. (2008).spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.eprintshttp://bdigital.unal.edu.co/38128/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/73652
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialspa
dc.relationhttp://revistas.unal.edu.co/index.php/agrocol/article/view/40544spa
dc.relation.ispartofUniversidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombianaspa
dc.relation.ispartofAgronomía Colombianaspa
dc.relation.ispartofseriesAgronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965
dc.relation.referencesAngel, Jorge Evelio and Hernández, Erick Geovanni and Herrera, Néstor Andrés and Gómez, Linda Yhiset and Castro, Ángela Patricia and Sepúlveda, Adriana Milena and Ebratt, Everth Emilio (2014) Comparison of dna extraction methods for detection of citrus huanglongbing in colombia. Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 Agronomía Colombiana; Vol. 32, núm. 1 (2014); 7-13 2357-3732 0120-9965 .spa
dc.rightsDerechos reservados - Universidad Nacional de Colombiaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.proposalBiologia molecularspa
dc.subject.proposalAgronomiaspa
dc.subject.proposalCandidatus Liberibacterspa
dc.subject.proposalDiaphorina citrispa
dc.subject.proposalPsyllidaespa
dc.subject.proposalvectors.spa
dc.subject.proposalFitopatologíaspa
dc.subject.proposalentomologíaspa
dc.titleComparison of dna extraction methods for detection of citrus huanglongbing in colombiaspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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