Caracterización molecular de aislamientos de Vibrio spp., recuperados de cuerpos de agua en Colombia y recuperados a través de la vigilancia por laboratorio de Microbiología del Instituto Nacional de Salud (INS)
| dc.contributor.advisor | Wiesner Reyes, Magdalena | spa |
| dc.contributor.advisor | Leal Castro, Aura Lucia | spa |
| dc.contributor.author | Sánchez Benavides, Lizeth Paola | spa |
| dc.contributor.researchgroup | Grupo en Microbiologia-INS | spa |
| dc.date.accessioned | 2020-08-19T22:18:09Z | spa |
| dc.date.available | 2020-08-19T22:18:09Z | spa |
| dc.date.issued | 2020-02-08 | spa |
| dc.description.abstract | The genus Vibrio spp has gained relevance worldwide due to the increase in aquatic environments and causing clinical cases called vibriosis. Currently the distribution and abundance of this genus in Colombia is unknown. The objective of this work is to identify and genotypically characterize isolates of V. cholerae, V. parahaemolyticus, V. alginolyticus, V. fluvialis and V. vulnificus, recovered from environmental samples (bodies of water) and clinical samples (suspected cases for Vibrio spp.). To do this, the search for the 5 included species was carried out using multiplex PCR. In addition, the virulence genes (ctxA, tcp, nanH, hlyA), (tdh, trh), vfh and (vcgC or vcgE), in each species respectively, were screened. the clonal relationship between the species will be established using PFGE. 240 isolates were evaluated: 195 of environmental origin collected by INVEMAR in 2018 and 2019 and sent to the INS from 2010 to 2019, and 45 of clinical origin sent to the INS in the same period. Of the total, 168 were identified and classified as one of the 5 white species. At the prevailing environmental level, V. parahaemolyticus (n = 41) and V. alginolyticus (n = 28), while at the prevailing clinical level, V. cholerae No O1 / O139 (n = 29) and V. fluvialis (n = 23). Some isolates carried virulence genes such as: nanH (43%), hlyA (54.5%), tdh (16.4%), vfh (96%), vcgC (85.7%) or vcgE (14.2%). All statistical species have great genetic diversity with the presence of some circulating clones in the country. The presence and distribution of 5 potentially pathogenic Vibrio species in the country in environmental and clinical samples was confirmed, which could affect as a public health problem. | spa |
| dc.description.abstract | El género Vibrio spp, ha cobrado relevancia a nivel mundial debido al incremento en ambientes acuáticos y causando casos clínicos denominados vibriosis. En la actualidad se desconoce la distribución y abundancia de este género en Colombia. El objetivo de este trabajo es identificar y caracterizar genotípicamente aislamientos de V. cholerae, V. parahaemolyticus, V. alginolyticus, V. fluvialis y V. vulnificus, recuperados de muestras ambientales (cuerpos de agua) y muestras clínicas (casos sospechosos para Vibrio spp.). Para ello se realiza búsqueda de las 5 especies mencionadas mediante PCR multiplex, adicionalmente se tamizaron los genes de virulencia (ctxA, tcp, nanH, hlyA), (tdh, trh), vfh y (vcgC o vcgE), en cada especie respectivamente y se estableció la relación clonal entre las especies mediante PFGE. Se evaluaron 240 aislamientos: 195 de origen ambiental recolectados por INVEMAR en 2018 y 2019 y remitidos al INS de 2010 a 2019, y 45 de origen clínico remitidos al INS en el mismo periodo. Del total, 168 se identificaron y clasificaron como alguna de las 5 especies blanco. A nivel ambiental predomino V. parahaemolyticus (n=41) y V. alginolyticus (n=28), mientras que a nivel clínico predomino V. cholerae No O1/O139 (n=29) y V. fluvialis (n=23). Algunos aislamientos fueron portadores de genes de virulencia como: nanH (43%), hlyA (54.5%), tdh (16,4%), vfh (96%), vcgC (85.7%) o vcgE (14.2%). Todas las especies mostraron gran diversidad genética con presencia de algunos clones circulantes en el país. Se confirmó la presencia y distribución de 5 especies potencialmente patogénicas de Vibrio en el país en muestras ambientales y clínicas, lo que podría considerarse como un problema de salud pública. | spa |
| dc.description.additional | Línea de Investigación: Biología molecular de agentes infecciosos | spa |
| dc.description.degreelevel | Maestría | spa |
| dc.description.project | “Vibrio spp en reservorios ambientales como potenciales focos de colera y vibriosis” | spa |
| dc.description.sponsorship | Colciencias | spa |
| dc.format.extent | 99 | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.citation | Sanchez, L.P. (2020). Caracterización molecular de aislamientos de Vibrio spp., recuperados de cuerpos de agua en Colombia y recuperados a través de la vigilancia por laboratorio de Microbiología del Instituto Nacional de Salud (INS) (Tesis de maestría). Universidad Nacional de Colombia, Bogotá, Colombia. | spa |
| dc.identifier.uri | https://repositorio.unal.edu.co/handle/unal/78100 | |
| dc.language.iso | spa | spa |
| dc.publisher.branch | Universidad Nacional de Colombia - Sede Bogotá | spa |
| dc.publisher.program | Bogotá - Ciencias - Maestría en Ciencias - Microbiología | spa |
| dc.relation.references | Abana, D., Gyamfi, E., Dogbe, M., Opoku, G., Opare, D., Boateng, G., y Mosi, L. (2019). Investigating the virulence genes and antibiotic susceptibility patterns of Vibrio cholerae O1 in environmental and clinical isolates in Accra, Ghana. Bmc Infectious Deseases. 19 (1), 76. | spa |
| dc.relation.references | Agudelo, C.A. (1994). Cólera en Colombia. Colombia: Ciencia Y Tecnología. 12 (4). Alam, M. T., Weppelmann, T.A., Longini, I., De Rochars, V.M., Morris, J.G. y Ali, A. (2015). Increased isolation frequency of toxigenic Vibrio cholerae O1 from environmental monitoring sites in Haiti. Plos One, 10 (4), E0124098. | spa |
| dc.relation.references | Almagro, S. y Boyd, E. (2009). Sialic acid catabolism confers a competitive advantage to pathogenic Vibrio cholerae in the mouse intestine. Infect Immun, 77 (9), 3807-3816. | spa |
| dc.relation.references | Bahamonde, C y Stuardo, V. La epidemia de cólera en América Latina: reemergencia y morbimortalidad. 2013;33(1):40–6. | spa |
| dc.relation.references | Baker, C., Trinanes, J., Gonzalez, N. Y Martinez, J. (2017). Non-Cholera Vibrios: The Microbial Barometer of Climate Change. Trends Microbiol, 25 (1), 76-84. | spa |
| dc.relation.references | Banerjee, R., Das, B., Nair, B., Basak, S. (2014). Dynamics in Genome Evolution of Vibrio Cholerae. Infect Genet Evol, 23, 32-41. | spa |
| dc.relation.references | Bej, A.K., Patterson, D.P., Brasher, C.W., Vickery, M.C., Jones, D.D. y Kaysner, C.A. (1999). Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex pcr amplification of tlh, tdh and trh. J Microbiol Methods, 36 (3), 215-225. | spa |
| dc.relation.references | Bharati, K. y Ganguly, N.K. (2011). Cholera toxin: A paradigm of a multifunctional protein. Indian J Med Res, 133 (2), 179-187. | spa |
| dc.relation.references | Bier, N., Bechlars, S., Diescher, S., Klein, F., Hauk, G., Duty, O. y Dieckmann, R. (2013). Genotypic diversity and virulence characteristics of clinical and environmental Vibrio vulnificus isolates from the baltic sea region. Appl Environ Microbiol, 79 (12), 3570-3581. | spa |
| dc.relation.references | Bou, G., Fernández, A., García, C., Sáez, J. y Valdezate, S. (2011). Métodos de identificación bacteriana en el laboratorio de microbiología. Enfermedades Infecciosas Y Microbiología Clínica, 29 (8), 601-608. | spa |
| dc.relation.references | Boucher, Y. y Stokes, H. (2006). The roles of lateral gene transfer and vertical descent in Vibrio evolution. Thompson F.L Et Al (Ed), The Biology of Vibrios. Washington, D.C: Asm Press. | spa |
| dc.relation.references | Caffer, M., Terragno, R., González, S., Viñas, M., Pichel, M. y Binsztein, N. (2007). Manual de procedimientos-aislamiento, identificación y caracterización de Vibrio cholerae.: Instituto Nacional De Enfermedades Infecciosas A.N.L.I.S. “Dr. Carlos G. Malbrán”: Centro Regional De Referencia Del Who Global Salm Surv Para América Del Sur. Recuperado De: Http://Bvs.Panalimentos.Org/Local/File/Manual_Vibrio_Cholerae_Aislamiento_Caracterizaci%A2n_2008.Pdf | spa |
| dc.relation.references | Castañón, C.A., Enríquez, F. y Figueroa, P. (2015). Efecto citotóxico de la proteína VCC de Vibrio cholerae en células Hek-293. Evidencia Médica E Investigación En Salud. 8 (3), 111-116. | spa |
| dc.relation.references | Ceccarelli, D., Chen, A., Hasan, N. A., Rashed, S. M., Huq, A. y Colwell, R. R. (2015). Non-O1/Non-O139 Vibrio cholerae carrying multiple virulence factors and V. cholerae O1 in the Chesapeake Bay, Maryland. Appl Environ Microbiol, 81 (6), 1909-1918. | spa |
| dc.relation.references | Centers for Diseases, Control and Prevention [CDC]. (2014). National Enteric Disease Surveillance: Covis Annual Summary, 2014. (2016). Recuperado de: Https://Www.Cdc.Gov/Nationalsurveillance/Pdfs/Covis-Annual-Summary-2014-508c.Pdf | spa |
| dc.relation.references | Chakraborty, R., Chakraborty, S., Keya. D, Sinha, S., Mukhopadhyay, A.K., Khanam, J., Ramamurthy T., Takeda Y., Bhattacharya S.K. y Nair G. B. (2005). Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea. J Med Microbiol, 54 (8), 707-716. | spa |
| dc.relation.references | Chakraborty, R., Sinha, S., Mukhopadhyay, A., Asakura, M., Yamasaki, S., Bhattacharya, S., Nair G.B. y Ramamurthy, T. (2006). Species-specific identification of Vibrio fluvialis by pcr targeted to the conserved transcriptional activation and variable membrane tether regions of the toxR gene. J Med Microbiol, 55 (6), 805-808. | spa |
| dc.relation.references | Chakraborty, S., Mukhopadhyay, A. K., Bhadra, R. K., Ghosh, A. N., Mitra, R., Shimada, T., Yamasaki, S. . . .Colwell, R. R. (2000). Virulence genes in environmental strains of Vibrio cholerae. Appl Environ Microbiol, 66 (9), 4022-4028. | spa |
| dc.relation.references | Chen, A., Hasan, N., Haley, B., Taviani, E., Tarnowski, M., …Colwell, R.R. (2017). Characterization of pathogenic Vibrio parahaemolyticus from the Chesapeake Bay, Maryland. Front Microbiol, 15 (8), 2460. | spa |
| dc.relation.references | Chen, C.Y., Wu, K.M., Chang, Y.C., Chang, C.H., Tsai, H.C., Liao, T.L., . . . Tsai, S.F. (2003). Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res, 13 (12), 2577-2587. | spa |
| dc.relation.references | Chowdhury, G., Joshi, S., Bhattacharya, S., Sekar, U., Birajdar, B., Bhattacharyya, A., Sumio, S., Y Ramamurthy, T. (2016). Extraintestinal infections caused by non-toxigenic Vibrio cholerae Non-O1/Non-O139. Front Microbiol, 7, 144. | spa |
| dc.relation.references | Chowdhury, G., Pazhani, G. P., Dutta, D., Guin, S., Dutta, S., Ghosh, S., . . . Ramamurthy, T. (2012). Vibrio fluvialis in patients with diarrhea, Kolkata, India. Emerg Infect Dis, 18 (11), 1868-1871. | spa |
| dc.relation.references | Chowdhury, N.R., Chakraborty, S., Ramamurthy, T., Nishibuchi, M., Yamasaki, S., Takeda, Y. y Nair, G.B. (2000). Molecular evidence of clonal Vibrio parahaemolyticus pandemic strains. Emerg Infect Dis, 6 (6), 631-636. | spa |
| dc.relation.references | Chunga, J.C. (2018). Vibrio cholerae y Vibrio parahaemolyticus toxigénicos en cuerpos de agua de Piura. (Tesis De Pregrado). Universidad Nacional De Piura, Peru. | spa |
| dc.relation.references | Chen, A. (2017). Vibrios in the environment: an investigation of environmental Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio cholerae (Tesis De Doctorado). University Of Maryland. Estados Unidos. | spa |
| dc.relation.references | Cobos, Y.T. (2019). Caracterización fenotípica de aislamientos de Vibrio spp. recuperados entre los años 2016-2019 de muestras clínicas y de agua para consumo humano de la vigilancia intensificada por laboratorio, así como de cuerpos de agua en zonas costeras en Colombia. (Tesis de pregrado) Universidad Antonio Nariño, Colombia. | spa |
| dc.relation.references | Cooper, K.L., Luey, C.K., Bird, M., Terajima, J., Nair, G.B., Kam, K.M., . . . y Ribot, E.M. (2006). Development And validation of a pulsenet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae. Foodborne Pathog Dis, 3 (1), 51-58. | spa |
| dc.relation.references | Croci, L., Suffredini, E., Cozzi, L., Toti, L., Ottaviani, D., Pruzzo, C., . . . Mioni, R. (2007). Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus. J Appl Microbiol, 102 (1), 229-237. | spa |
| dc.relation.references | Dalsgaard, A., Serichantalergs, O., Forslund, A., Lin, W., Mekalanos, J., Mintz, E., . . . Wells, J. G. (2001). Clinical and environmental isolates of Vibrio cholerae serogroup O141 carry the ctx phage and the genes encoding the toxin-coregulated pili. J Clin Microbiol, 39 (11), 4086-4092. | spa |
| dc.relation.references | Davis, B.J., Jacobs, J.M., Davis, M.F., Schwab, K.J., De Paola, A. y Curriero, F.C. (2017). Environmental determinants of Vibrio parahaemolyticus in the Chesapeake Bay. Appl Environ Microbiol, 83 (21), E01147-17. | spa |
| dc.relation.references | De Menezes, F., Rodriguez, M., De Carvalho, F., Rebouças, R., Costa, R., De Sousa, O., . . . Vieira, R. (2017). Pathogenic Vibrio species isolated from estuarine environments (Ceará, Brazil) - antimicrobial resistance and virulence potential profiles. An. Acad. Bras. Ciênc, 89 (2), 1175-1188. | spa |
| dc.relation.references | Di Pinto, A., Ciccarese, G., Tantillo, G., Catalano, D. y Forte, V. (2005). A collagenase-targeted multiplex pcr assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus. J Food Prot, 68 (1), 150-153. | spa |
| dc.relation.references | Dickinson, G., Lim, K. Y., Y Jiang, S. C. (2013). Quantitative Microbial Risk Assessment of Pathogenic Vibrios In Marine Recreational Waters of Southern California. Appl Environ Microbiol, 79 (1), 294-302. | spa |
| dc.relation.references | Elmahdi, S., Parveen, S., Ossai, S., Dasilva, L.V., Jahncke, M., Bowers, J. y Jacobs, J. (2018). Vibrio parahaemolyticus and Vibrio vulnificus recovered from oysters during an oyster relay study. Appl Environ Microbiol, 84 (3), E01790-17. | spa |
| dc.relation.references | Eneva, R., Engibarov, S., Strateva, T., Abrashev, R. y Abrashev, I. (2011). Biochemical studies on the production of neuraminidase by environmental isolates of Vibrio cholerae non-o1 from Bulgaria. Can J Microbiol, 57 (7), 606-610. | spa |
| dc.relation.references | Esteves, K., Hervio-Heath, D., Mosser, T., Rodier, C., Tournoud, M. G., Jumas-Bilak, E., . . . Monfort, P. (2015). Rapid Proliferation of Vibrio Parahaemolyticus, Vibrio Vulnificus, And Vibrio Cholerae During Freshwater Flash Floods in French Mediterranean Coastal Lagoons. Appl Environ Microbiol, 81 (21), 7600-7609. | spa |
| dc.relation.references | Fabbro, C., Cataletto, B. y Del Negro, P. (2010). Detection of pathogenic vibrio parahaemolyticus through biochemical and molecular-based methodologies in coastal waters of the gulf of Trieste (North Adriatic Sea). Fems Microbiol Lett, 307 (2), 158-164. | spa |
| dc.relation.references | Faruque, S., Albert M.J. y Mekalanos, J.J. (1998). Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Biol Rev, 62 (4), 1301-1314. | spa |
| dc.relation.references | Faruque, S. M. y Nair, G. B. (2002). Molecular Ecology Of Toxigenic Vibrio cholerae. Microbiol Immunol, 46 (2), 59-66. | spa |
| dc.relation.references | Fernández F.S., y Alonso, G. (2009). Colera Y Vibrio cholerae. Inhrr, 40 (2), 50-69. | spa |
| dc.relation.references | Fields, P., Popovic, T., Wachsmuth, K. y Olsvik, O. (1992). Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J Clin Microbiol, 30 (8), 2118-2121. | spa |
| dc.relation.references | Galen, J.E., Ketley, J.M., Fasano, A., Richardson, S.H., Wasserman, S.S. y Kaper, J.B. (1992). Role of Vibrio cholerae neuraminidase in the function of cholera toxin. Infect Immun, 60 (2), 406-415. | spa |
| dc.relation.references | García, E., Hernández, A., Herrero J.A. y Gómez, J. (2010). Cólera y otras infecciones del género Vibrio. Medicine, 10 (52), 3489-3496. | spa |
| dc.relation.references | Gargouti A.S., Ab-Rashid M.N.K., Ghazali M.F., Mitsuaki N., Haresh K.K. y Radu S. (2015). Detection of tdh and trh toxic genes in Vibrio alginolyticus strain from mantis shrimp (Oratosquilla Oratoria). Journal Of Nutrition & Food Sciences, 2015. 5 (5), 405. | spa |
| dc.relation.references | Garibyan, L., y Avashia, N. (2013). Research techniques made simple: polymerase chain reaction (Pcr). J Invest Dermatol, 133 (3), 1-4. | spa |
| dc.relation.references | Gavilán, R.G. y Martínez, J. (2011). Factores ambientales vinculados con la aparición y dispersión de las epidemias de Vibrio en América del Sur. Revista Peruana De Medicina Experimental Y Salud Pública, 28 (1), 109-115. | spa |
| dc.relation.references | Gennari, M., Ghidini, V., Caburlotto, G. y Lleo, M. (2012). Virulence genes and pathogenicity islands in environmental Vibrio strains nonpathogenic to humans. Fems Microbiol Ecol, 82 (3), 563-573. | spa |
| dc.relation.references | Gomez, B. y Roque, A 2006. Isolation, enumeration, and preservation of the Vibrionaceae-Chapter 2, The Biology of Vibrios. Washington, D.C. ASM Press. | spa |
| dc.relation.references | González, S., Villagra, A., Pichel, M., Figueroa, S., Merletti, G., Caffer, M., . . . Binsztein, N. (2009). Caracterización de aislamientos de Vibrio cholerae No-O1, No-O139 asociados a cuadros de diarrea. Rev. Argent. Microbiol., 41 (1), 111-119. | spa |
| dc.relation.references | González, N., Whitney, B., Jaykus, L. y Depaola, A. (2007). Comparison of direct genome restriction enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data. Appl Environ Microbiol, 73 (22), 7494-7500. | spa |
| dc.relation.references | González, N., Blackstone, G.M. y De Paola, A. (2006). Characterization of a Vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus. Appl Environ Microbiol, 72 (12), 7925-7929. | spa |
| dc.relation.references | Guin, S., Saravanan, M., Anjay, Chowdhury, G., Pazhani, G., Ramamurthy, T. y Das, S. (2019). Pathogenic Vibrio parahaemolyticus in diarrhoeal patients, fish and aquatic environments and their potential for inter-source transmission. Heliyon, 5 (5), E01743. | spa |
| dc.relation.references | Han, J.H., Lee, J.H., Choi, Y.H., Park, J.H., Choi, T.J. y Kong, I.S. (2002). Purification, characterization and molecular cloning of Vibrio fluvialis hemolysin. Biochim Biophys Acta, 1599 (1-2), 106-114. | spa |
| dc.relation.references | Hasan, N. A., Ceccarelli, D., Grim, C. J., Taviani, E., Choi, J., Sadique, A., . . . Colwell, R. R. (2013). Distribution of virulence genes in clinical and environmental Vibrio cholerae strains in bangladesh, Appl Environ Microbiol. 79 (18), 5782-5. | spa |
| dc.relation.references | Hernández, C., Ulloa, J. Vergara, J., Espejo, R., Cabello, F. (2005). Infecciones por Vibrio parahaemolyticus e intoxicaciones por algas: problemas emergentes de salud pública en Chile. Rev Méd Chile. 133, 1081-1088. | spa |
| dc.relation.references | Hernandez, M., Alvarez, A., Juarez, P., Bonifacio, N., Quesada, C., Vazquez, C. y Quinones, E. (2016). Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus. Int Microbiol, 19 (4), 191-198. | spa |
| dc.relation.references | Horseman, M. y Surani, S. (2011). A comprehensive review of Vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection. International Journal of Infectious Diseases, 15 (3), 157-166. | spa |
| dc.relation.references | Hossain, M.T., Kim, Y.O. y Kong, I.S. (2013). Multiplex pcr for the detection and differentiation of Vibrio parahaemolyticus strains using the groel, tdh and trh genes. Mol Cell Probes, 27 (5-6), 171-175. | spa |
| dc.relation.references | Hu, D., Liu, B., Feng., Ding, P., Guo, X., Wang, M., Cao, B., Reeves, P.R. y Wang, L. (2016). Origins of the current seventh cholera pandemic. Proc Natl Acad Sci U S A, 29 (48), 7730-7739. | spa |
| dc.relation.references | Huehn, S., Eichhorn, C., Urmersbach, S., Breidenbach, J., Bechlars, S., Bier, N., . . . Strauch, E. (2014). Pathogenic Vibrios In Environmental, Seafood and Clinical Sources in Germany. Int J Med Microbiol, 304 (7), 843-850. | spa |
| dc.relation.references | Igbinosa, E.O. y Okoh, A.I. (2010). Vibrio fluvialis: An unusual enteric pathogen of increasing public health concern. Int J Environ Res Public Health, 7 (10), 3628-3643. | spa |
| dc.relation.references | Instituto Nacional De Salud. (2011). Protocolo De Vigilancia En Salud Pública-Cólera. Recuperado de: Https://Cruevalle.Org/Files/Pro-Colera.PdfInstit | spa |
| dc.relation.references | Instituto Nacional de Salud. (2013). Vigilancia fenotípica y genotípica de Vibrio cholerae 2010 – 2013. Recuperado de: https://www.ins.gov.co/buscador-eventos/Informacin%20de%20laboratorio/Vigilancia%20C%C3%B3lera%20Colombia%202013.pdf | spa |
| dc.relation.references | Instituto Nacional de Salud. (2017). Guía para la vigilancia por laboratorio de Vibrio cholerae. Recuperado de: https://www.ins.gov.co/buscador-eventos/Informacin%20de%20laboratorio/Gu%C3%ADa%20para%20la%20vigilancia%20por%20laboratorio%20de%20Vibrio%20cholerae.pdf | spa |
| dc.relation.references | Irenge, L., Ambroise, J., Mitangala, P., Bearzatto, B., Kabangwa, R., Durant, J. y Gala, J. (2019). Genomic analysis of pathogenic isolates of Vibrio cholerae from Eastern Democratic Republic of the Congo (2014-2017). Biorxiv, 708-735. | spa |
| dc.relation.references | Jeong, H., Kim, J., Jeon, S., Park, M. y Kim, S. (2011). Genotypic characterization of Vibrio Vulnificus clinical isolates in Korea. Osong Public Health Res Perspect, 2 (1), 8-14. | spa |
| dc.relation.references | Johnson, C.N. (2015). Influence of Environmental Factors on Vibrio Spp. In Coastal Ecosystems. Microbiol Spectr, 3 (3). | spa |
| dc.relation.references | Jones, M K. y Oliver, J.D. (2009). Vibrio vulnificus: disease and pathogenesis. Infect Immun, 77 (5), 1723-1733. | spa |
| dc.relation.references | Kahler, A. M., Haley, B. J., Chen, A., Mull, B. J., Tarr, C. L., Turnsek, M., . . . Hill, V. R. (2015). Environmental surveillance for toxigenic Vibrio cholerae in surface waters of Haiti. Am J Trop Med Hyg, 92 (1), 118-125. | spa |
| dc.relation.references | Kam, K.M., Luey, C.K., Parsons, M.B., Cooper, K.L., Nair, G.B., Alam, M., . . . Swaminathan, B. (2008). Evaluation and validation of a pulsenet standardized pulsed-field gel electrophoresis protocol for subtyping Vibrio parahaemolyticus: an international multicenter collaborative study. J Clin Microbiol, 46 (8), 2766-2773. | spa |
| dc.relation.references | Khimmakthong, U. y Noochu, S. (2017). Detection of three pathogenic Vibrio species using multiplex polymerase chain reaction (Multiplex-Pcr). Science & Technology Asia, 22 (4). | spa |
| dc.relation.references | Kim, H.J., Ryu, J.O., Lee, S.Y., Kim, E.S. y Kim, H.Y. (2015). Multiplex pcr for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. Bmc Microbiol, 15, 239. | spa |
| dc.relation.references | Kokashvili, T., Whitehouse, C.A., Tskhvediani, A., Grim, C.J., Elbakidze, T., Mitaishvili, N., . . . Tediashvili, M. (2015). Occurrence and diversity of clinically important Vibrio species in the aquatic environment of georgia. Front Public Health, 3, 232. | spa |
| dc.relation.references | Koralage, M.S., Alter, T., Pichpol, D., Strauch, E., Zessin, K.H. y Huehn, S. (2012). Prevalence and molecular characteristics of Vibrio spp. isolated from preharvest shrimp of the north western province of Sri Lanka. Journal of Food Protection, 75 (10), 1846-50. | spa |
| dc.relation.references | Kothary, M.H., Lowman, H., Mccardell, B.A. y Tall, B.D. (2003). Purification and characterization of enterotoxigenic el tor-like hemolysin produced by Vibrio fluvialis. Infect Immun, 71 (6), 3213-3220. | spa |
| dc.relation.references | Krebs, S.J. y Taylor, R.K. (2011). Protection and attachment of Vibrio cholerae mediated by the toxin-coregulated pilus in the infant mouse model. J Bacteriol, 193 (19), 5260-5270. | spa |
| dc.relation.references | Kurdi, M., Mutalib, S., Ghani, M. y Aldulaimi, M. (2014). A review of important virulence factors of Vibrio vulnificus. Current Research Journal of Biological Sciences, 6 (2), 76-88. | spa |
| dc.relation.references | Leyva, V., Puig, Y., Espino, M., Pereda, G., Portela, N., Morejón, P. y Roble, O. (2013). Especies patógenas de Vibrio aisladas en alimentos de origen marino. Revista Cubana de Alimentación y Nutrición. 23 (1). | spa |
| dc.relation.references | Li, J., Lim, M.S., Li, S., Brock, M., Pique, M.E., Woods, V.L. y Craig, L. (2008). Vibrio cholerae toxin-coregulated pilus structure analyzed by hydrogen/deuterium exchange mass spectrometry. Structure, 16 (1), 137-148. | spa |
| dc.relation.references | Liang, P., Cui, X., Du, X., Kan, B. y Liang, L. (2013). The virulence phenotypes and molecular epidemiological characteristics of Vibrio fluvialis in china. Gut Pathogens, 5 (1), 1-11. | spa |
| dc.relation.references | Lockwood, D.E., Kreger, A.S. y Richardson, S.H. (1982). Detection of toxins produced by Vibrio fluvialis. Infect Immun, 35 (2), 702-708. | spa |
| dc.relation.references | Luo, Y., Ye, J., Jin, D., Ding, G., Zhang, Z., Mei, L., . . . Lan, R. (2013). Molecular analysis of Non-O1/Non-O139 Vibrio cholerae isolated from hospitalised patients in China. Bmc Microbiol, 13, 52. | spa |
| dc.relation.references | López, A., Manjarrez, G., Herrera, L., Montes, A., Olascuaga, Y., Ortega, R. (2010). Estudio piloto para el aislamiento de Vibrio spp en la ostras (Crassostrea Rhizophorae) capturadas en la ciénaga de la virgen, Cartagena, Colombia. Rev Salud Publica Nutr, 11, (1). | spa |
| dc.relation.references | Madhusudana, R.B. y Surendran, P.K. (2013). Detection of ctx gene positive Non-O1/Non-O139 V. cholerae in shrimp aquaculture environments. J Food Sci Technol, 50 (3), 496-504. | spa |
| dc.relation.references | Mahoney, J.C., Gerding, M.J., Jones, S.H. y Whistler, C.A. (2010). Comparison of the pathogenic potentials of environmental and clinical Vibrio parahaemolyticus strains indicates a role for temperature regulation in virulence. Appl Environ Microbiol, 76 (22), 7459-7465. | spa |
| dc.relation.references | Martinelli Filho, J.E., Lopes, R.M., Rivera, I.N. y Colwell, R.R. (2016). Are natural reservoirs important for cholera surveillance? the case of an outbreak in a Brazilian estuary. Lett Appl Microbiol, 63 (3), 183-188. | spa |
| dc.relation.references | Martinez, J., Lozano, A., Viña, A., De Novoa, J. y Garcia, O. (2006). Differences in the api 20e biochemical patterns of clinical and environmental Vibrio parahaemolyticus isolates. Fems Microbiol Lett, 255 (1), 75-81. | spa |
| dc.relation.references | Ministerio de Salud. (2018). Circular externa conjunta No. 027 de 2018- Directrices para el fortalecimiento de las acciones de prevención, vigilancia, atención y control de cólera. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/circular-027-de-2018.pdf | spa |
| dc.relation.references | Montaño, L., Díaz, P., Duarte, C., Moreno, J., Porras, P., Ríos, R., . . . Wiesner, M. (2017). diversidad filogenética de aislamientos de Vibrio cholerae recuperados en Colombia, 1991-2014. Bogota-Colombia. Presentaciones En Cartel. Recuperado de: Https://Www.Revistabiomedica.Org/Index.Php/Biomedica/Article/View/4200/3752 | spa |
| dc.relation.references | Moore, S., Thomson, N., Mutreja, A. y Piarroux, R. (2014). Widespread epidemic cholera caused by a restricted subset of Vibrio cholerae clones. Clin Microbiol Infect, 20 (5), 373-379. | spa |
| dc.relation.references | Morris, J. y Achenson, D. (2003). Cholera and other types of vibriosis: a story of human pandemics and oysters on the half shell. Clinical Infectious Diseases, 37 (2), 272-280. | spa |
| dc.relation.references | Mustapha, S., Mustapha, E.M., Brahim, B. y Cohen, N. (2012). Characterization of Vibrio alginolyticus trh positive from mediterranean environment of Tamouda Bay (Morocco). World Environment, 2 (4), 76-80. | spa |
| dc.relation.references | Mustapha, S, Mustapha, M y Nozha. C. (2013). Vibrio alginolyticus: An emerging pathogen of foodborne diseases. Maejo international journal of science and technology. 2. 302-309. | spa |
| dc.relation.references | Neogi, S.B., Chowdhury, N., Asakura, M., Hinenoya, A., Haldar, S., Saidi, S.M., Kogure, K., Lara, R.J. y Yamasaki, S. (2010). A highly sensitive and specific multiplex pcr assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus. Lett Appl Microbiol. 51 (3), 293-300 | spa |
| dc.relation.references | Newton, A.E., Garrett, N., Stroika, S.G., Halpin, J.L., Turnsek, M., Mody, R. K. Centers for Disease Control and Prevention (CDC) (2014). Increase in Vibrio parahaemolyticus infections associated with consumption of atlantic coast shellfish--2013. Mmwr. Morbidity and Mortality Weekly Report, 63 (15), 335–336. | spa |
| dc.relation.references | Newton, A., Kendall, M., Vugia, D.J., Henao, O.L. y Mahon, B.E. (2012). Increasing rates of vibriosis in the united states, 1996-2010: review of surveillance data from 2 systems. clinical infectious diseases: An official publication of the infectious disease’s society of America, 54 Suppl 5(0 5), S391–S395. | spa |
| dc.relation.references | Nhung, P.H., Ohkusu, K., Miyasaka, J., Sun, X.S. y Ezaki, T. (2007). Rapid and specific identification of 5 human pathogenic Vibrio species by multiplex polymerase chain reaction targeted to dnaJ gene. Diagn Microbiol Infect Dis. 59 (3):271-5. | spa |
| dc.relation.references | Organizacion Panamericana de la Salud. (2010). Procedimientos para la identificación de Vibrio cholerae en el laboratorio de microbiología. Recuperado de: https://www.paho.org/hq/dmdocuments/2010/Procedimiento_identificacion_V_cholera_LAB.pdf | spa |
| dc.relation.references | O'shea, Y.A., Reen, F.J., Quirke, A.M. y Boyd, E.F. (2004). Evolutionary genetic analysis of the emergence of epidemic Vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles. J Clin Microbiol, 42 (10), 4657-4671. | spa |
| dc.relation.references | Oliver, J. D. (2005). Wound infections caused by Vibrio vulnificus and other marine bacteria. Epidemiol Infect, 133 (3), 383-391. | spa |
| dc.relation.references | Olson, R. y Gouaux, E. (2005). Crystal structure of the Vibrio cholerae cytolysin (VCC) pro-toxin and its assembly into a heptameric transmembrane pore. J Mol Biol, 350 (5), 997-1016. | spa |
| dc.relation.references | Parsons, M.B., Cooper, K.L., Kubota, K.A., Puhr, N., Simington, S., Calimlim, P. S., . . . Ribot, E. M. (2007). Pulsenet usa standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio parahaemolyticus. Foodborne Pathog Dis, 4 (3), 285-292. | spa |
| dc.relation.references | Parveen, S., Hettiarachchi, K.A., Bowers, J.C., Jones, J.L., Tamplin, M.L., Mckay, R., . . . Depaola, A. (2008). Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters. Int J Food Microbiol, 128 (2), 354-361. | spa |
| dc.relation.references | Percival, S., y Williams, D. (2014). Chapter Twelve - Vibrio. (Eds.), Microbiology of Waterborne Diseases (Second Edition) (Pp. 237-248). London: Academic Press. | spa |
| dc.relation.references | Pinto, M.F., Baptista, T. y Neves, C.C. (2017). Development of a new multiplex-pcr tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda. Aquat. Living Resour, 30, 4. | spa |
| dc.relation.references | Rader, A.E. y Murphy, J.R. (1988). Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor Rv79(Hly+) and Rv79(Hly-) and classical 569b(Hly-). Infect Immun, 56(6), 1414-1419. | spa |
| dc.relation.references | Raghunath P. (2015). Roles of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) in Vibrio parahaemolyticus. Frontiers in Microbiology, 5, 805. | spa |
| dc.relation.references | Ramamurthy, T., Chowdhury, G., Pazhani, G. P. y Shinoda, S. (2014). Vibrio fluvialis: an emerging human pathogen. Front Microbiol, 5 (91). | spa |
| dc.relation.references | Raszl, S.M., Froelich, B.A., Vieira, C.R., Blackwood, A.D. y Noble, R.T. (2016). Vibrio parahaemolyticus and Vibrio vulnificus in South America: water, seafood and human infections. J Appl Microbiol, 121(5), 1201-1222. | spa |
| dc.relation.references | Rattanathumsakul, T., Suwanchairob, O., Hannarong, S., Wijit, W., Laosiritaworn, Y. y Swaddiwudhipong, W. (2019). Sequential clusters of multidrug-resistant cholera cases in the thai-myanmar border, 2015. Outbreak, Surveillance, Investigation & Response, 12 (2). | spa |
| dc.relation.references | Ren, C., Hu, C., Jiang, X., Sun, H., Zhao, Z., Chen, C., y Luo, P. (2013). Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems. Mol Cell Probes, 27 (3-4), 164-168. | spa |
| dc.relation.references | Ren, C., Hu, C., Luo, P., Chen, C., Jiang, X. y Wang, Q. (2008). Genotyping of Vibrio alginolyticus isolates from daya bay by infrequent-restriction-site pcr and pulsed-field gel electrophoresis. Mol Cell Probes, 22 (4), 267-271. | spa |
| dc.relation.references | Riaño, D., Valenzuela, E., Mantilla, J. Y Agudelo, C. (2003). Diversidad Genética Y Estructura De La Población De Vibrio Cholerae En Colombia. Revista Colombiana De Biotecnología, 1 (1), 36-44. | spa |
| dc.relation.references | Rivas, A., Luis, I., Piña, P., Gomez, B. y Lizárraga, M. (2018). Spatiotemporal distribution of Vibrio parahaemolyticus in relation to environmental parameters in a coastal lagoon on the Pacific coast of northwestern Mexico. Ciencias Marinas. 44. 141-153. | spa |
| dc.relation.references | Ribeiro, M., Matté, M., Dropa, M., Lopes, M. y Matté, G. (2011). Characterization of Vibrio parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil. Rev. Inst. Med. Trop. S. Paulo, 53 (4), 201-5. | spa |
| dc.relation.references | Rivera, I.N., Chun, J., Huq, A., Sack, R.B. y Colwell, R.R. (2001). Genotypes associated with virulence in environmental isolates of Vibrio cholerae. Appl Environ Microbiol, 67 (6), 2421-2429. | spa |
| dc.relation.references | Rodríguez, D. (2011). Determinación de Vibrio cholerae en tanques de aguas de lastre de buques internacionales que arribaron al terminal ultiboyas de ecopetrol y al puerto de Tumaco. Boletín Científico Cioh, 29 (0120-0542), 104-109. | spa |
| dc.relation.references | Rosche, T., Yano, Y. y Oliver, J. (2005). A rapid and simple pcr analysis indicates there are two subgroups of Vibrio vulnificus which correlate with clinical or environmental isolation. Microbiol Immunol, 49(4), 381-389. | spa |
| dc.relation.references | Rosche, T.M., Binder, E.A. y Oliver, J.D. (2010). Vibrio vulnificus genome suggests two distinct ecotypes. Environ Microbiol Rep, 2 (1), 128-132. | spa |
| dc.relation.references | Roux, F. L., Wegner, K. M., Baker-Austin, C., Vezzulli, L., Osorio, C. R., Amaro, C., . . . Huehn, S. (2015). The Emergence of Vibrio Pathogens in Europe: Ecology, Evolution, And Pathogenesis (Paris, 11–12th March 2015). Front Microbiol, 6, 830. | spa |
| dc.relation.references | Sanchez, J. y Holmgren, J. (2011). Cholera toxin - A foe and a friend. Indian J Med Res, 133 (2), 153–163. | spa |
| dc.relation.references | Serpa, F. (1992). Historia Del Cólera En Colombia. Biomedica, 12 (3-4), 95-101. | spa |
| dc.relation.references | Shirai, H., Nishibuchi, M., Ramamurthy, T., Bhattacharya, S.K., Pal, S.C. y Takeda, Y. (1991). Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae. J Clin Microbiol, 29 (11), 2517-2521. | spa |
| dc.relation.references | Siboni, N., Balaraju, V., Carney, R., Labbate, M. Y Seymour, J. R. (2016). Spatiotemporal Dynamics of Vibrio Spp. Within the Sydney Harbour Estuary. Front Microbiol, 7, 460. | spa |
| dc.relation.references | Tamayo, M., Koblavi, S., Grimont, F., Castaneda, E. Y Grimont, P. A. (1997). Molecular Epidemiology Of Vibrio Cholerae O1 Isolates From Colombia. J Med Microbiol, 46 (7), 611-616. | spa |
| dc.relation.references | Taneja, N., Mishra, A., Batra, N., Gupta, P., Mahindroo, J. y Mohan, B. (2019). Inland cholera in freshwater environs of North India. Vaccine. 68 (1), 63-72. | spa |
| dc.relation.references | Tra, V. T., Meng, L., Pichpol, D., Pham, N. H., Baumann, M., Alter, T. Y Huehn, S. (2016). Prevalence and Antimicrobial Resistance of Vibrio Spp. In Retail Shrimps in Vietnam. Berl Munch Tierarztl Wochenschr, 129 (1-2), 48-51. | spa |
| dc.relation.references | Uchiyama, H. (2000). Distribution of Vibrio species isolated from aquatic environments with TCBS agar. Environ Health Prev Med, 4 (4), 199-204. | spa |
| dc.relation.references | Valenzuela, E., Mantilla, J. A. Y Agudelo, C. (1997). Vibrio Cholerae Non-O1 In Colombia. Water Research, 31 (12), 3175-3177. | spa |
| dc.relation.references | Velazquez, J., León, N., Hernández, L., y Canizalez, A. (2014). Pandemic Vibrio Parahaemolyticus O3:K6 On the American Continent. Frontiers in Cellular and Infection Microbiology, 3, 110. | spa |
| dc.relation.references | Vezzulli, L., Grande, C., Reid, P. C., Helaouet, P., Edwards, M., Hofle, M. G., . . . Pruzzo, C. (2016). Climate Influence on Vibrio and Associated Human Diseases During the Past Half-Century in The Coastal North Atlantic. Proc Natl Acad Sci U S A, 113 (34), 5062-5071. | spa |
| dc.relation.references | Vezzulli, L., Pezzati, E., Brettar, I., Hofle, M. Y Pruzzo, C. (2015). Effects of Global Warming on Vibrio Ecology. Microbiol Spectr, 3 (3). | spa |
| dc.relation.references | Viñas, M.R. (2016). Vibrio cholerae No-O1 asociado a diarreas: factores de virulencia y diversidad genética de un patógeno emergente en Latinoamérica. (Maestria Microbiologia Molecular). Universidad Nacional De San Martín, Argentina. | spa |
| dc.relation.references | Vu, T. T., Alter, T. Y Huehn, S. (2018). Prevalence of Vibrio Spp. In Retail Seafood in Berlin, Germany. J Food Prot, 81 (4), 593-597. | spa |
| dc.relation.references | Wang, H., Tang, X., Su, Y.C., Chen, J. y Yan, J. (2017). Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 To 2014. Plos One, 12 (7), E0180335. | spa |
| dc.relation.references | Wang, R., Zhong, Y., Gu, X., Yuan, J., Saeed, A. y Wang, S. (2015). The pathogenesis, detection, and prevention of Vibrio parahaemolyticus. Front Microbiol, 6, 144. | spa |
| dc.relation.references | Wei, S., Zhao, H., Xian, Y., Hussain, M. y Wu, X. (2014). Multiplex pcr assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae with an internal amplification control. Diagn Microbiol Infect Dis, 79 (2), 115-118. | spa |
| dc.relation.references | Whitten, R. (2018). Vibrio cholerae. Recuperado de: Https://Www.Ncbi.Nlm.Nih.Gov/Books/Nbk526099/ | spa |
| dc.relation.references | Wiesner, M., Porras, P., Díaz, P., Díaz, R., Montaño, L.A., Díaz, L., Tuduri, R., Reyes, J., Campos, J., Moreno, J. y Duarte, C. (Manuscrito en preparación). Genome diversity of Vibrio cholerae O1 and non-O1/non-O139 isolates from Colombia in two periods. | spa |
| dc.relation.references | Wong, H., Chen, S., Chen, M., Oliver, J., Hor, L. y Tsai, W. (2004). Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States. Appl Environ Microbiol, 70 (9), 5153-5158. | spa |
| dc.relation.references | Wong, H. y Lin, C. (2001). Evaluation of typing of Vibrio parahaemolyticus by three pcr methods using specific primers. J Clin Microbiol, 39 (12), 4233-40. | spa |
| dc.relation.references | World Health Organization [WHO]. (2018). Cholera. Recuperado De: Http://Www.Who.Int/News-Room/Fact-Sheets/Detail/Cholera. | spa |
| dc.relation.references | Xie, Z., Hu, C., Chen, C., Zhang, L. y Ren, C. (2005). Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China. Letters in Applied Microbiology, 41 (2), 202-7. | spa |
| dc.relation.references | Yin, J.F., Wang, M.Y., Chen, Y. J., Yin, H.Q., Wang, Y., Lin, M.Q., . . . Hu, C.J. (2018). Direct detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio alginolyticus from clinical and environmental samples by a multiplex touchdown polymerase chain reaction assay. Surg Infect (Larchmt), 19 (1), 48-53. | spa |
| dc.relation.references | Zavala, A., Quiñones, E. y Vazquez, C. (2005). La vida obscura de Vibrio alginolyticus. Revista Digital Universitaria-Unam, 6 (4). | spa |
| dc.relation.references | Zhou, S., Hou, Z., Li, N. y Qin, Q. (2007). Development of a sybr green i real-time pcr for quantitative detection of Vibrio alginolyticus in seawater and seafood. J Appl Microbiol, 103 (5), 1897-1906. | spa |
| dc.rights | Derechos reservados - Universidad Nacional de Colombia | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.license | Atribución-NoComercial-SinDerivadas 4.0 Internacional | spa |
| dc.rights.spa | Acceso abierto | spa |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | spa |
| dc.subject.proposal | vibriosis | eng |
| dc.subject.proposal | vibriosis | spa |
| dc.subject.proposal | water bodies | eng |
| dc.subject.proposal | cuerpos de agua | spa |
| dc.subject.proposal | PCR multiplex | spa |
| dc.subject.proposal | multiplex PCR | eng |
| dc.subject.proposal | genes | spa |
| dc.subject.proposal | genes | eng |
| dc.subject.proposal | dinámica poblacional | spa |
| dc.subject.proposal | population dynamics | eng |
| dc.title | Caracterización molecular de aislamientos de Vibrio spp., recuperados de cuerpos de agua en Colombia y recuperados a través de la vigilancia por laboratorio de Microbiología del Instituto Nacional de Salud (INS) | spa |
| dc.type | Trabajo de grado - Maestría | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
| dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.version | info:eu-repo/semantics/acceptedVersion | spa |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |