Evaluación del efecto coadyuvante de 2H-cromen-2-ona para la prevención de convulsiones inducidas experimentalmente en ratones de laboratorio

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Autores

Núñez Medina, Ángela Viviana

Director

Guerrero Pabon, Mario Francisco

Tipo de contenido

Trabajo de grado - Maestría

Idioma del documento

Español

Fecha de publicación

2023

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Abstract

Epilepsy is a potentially chronic neurodegenerative disease that affects humans and animals. The majority of patients require the prescription of antiepileptic drugs (AEDs). However, around 30% of them do not present complete resolution of their seizures, which is why they are categorized as refractory patients, that is, individuals who, despite having a medical regimen, have no control over these. This condition affects their quality of life. Due to this particularity of pharmacological resistance, the goal of this work was to evaluate the effect of coumarin (2H-chromen-2-one) as an adjuvant in the prevention of seizures. In this work, the response of laboratory mice in models of seizure induction under the effects of antiepileptic drugs such as carbamazepine, valproic acid, and levetiracetam, the use of coumarin and the combination of drug and coumarin, was evaluated in stimulation high-intensity electroshock (MES), low-frequency model (6 Hz), and chemical induction with pentylenetetrazol (PTZ), additionally acute neurotoxicity was evaluated using the rolling axis test (Rotarod). From the evaluation of coumarin (2H-chromen-2-one), first of all, in the rolling axis test, it was found that there was no significant alteration in the motor skills of the individuals. Regarding the data obtained in the seizure induction trials, it was possible to determine that the combination of carbamazepine and coumarin had a potential anticonvulsant effect in the MES model, lower significance was found at the other models concerning the MES model. The data provided in the document suggest that the use of coumarin (2H-chromen-2-one) in combination with first-line prototype drugs in mouse models would exert anticonvulsant effects and a possible synergistic effect.

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