Efecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratas

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dc.contributor.advisorDueñas Gómez, Zulma Janethspa
dc.contributor.authorMurillo Murillo, Marisolspa
dc.date.accessioned2023-06-05T20:18:24Z
dc.date.available2023-06-05T20:18:24Z
dc.date.issued2023
dc.descriptionilustraciones, gráficasspa
dc.description.abstracta separación materna durante la lactancia (SMDL) en roedores (modelo de estrés crónico) altera procesos del desarrollo a nivel neuroendocrino y fisiológico, evidentes en la adolescencia y adultez asociados con la depresión, el abuso de sustancias, dificultades en procesos aprendizaje, ansiedad y trastornos de conductas alimentarias. En este sentido sistemas neuromoduladores endocannabinoide y opioides que actúan sobre la actividad del eje Hipotálamo Pituitaria Adrenal e inciden en áreas reguladoras de la conducta alimentaria y sistema de recompensa por acción de receptores cannabinoides CB1 y opioides tipo Mu podrían presentar alteraciones. Por lo anterior, el objetivo de este trabajo investigativo fue determinar el efecto de la SMDL y el consumo de sucralosa sobre la expresión de receptores de CB1 y Mu en corteza prefrontal medial, hipocampo, estriado, área tegmental ventral y la amígdala de ratas wistar adolescentes de ambos sexos. Metodología: Se utilizaron 35 ratas Wistar machos y hembras divididas en grupos con y sin SMDL (n=18; n:17) durante los días posnatal (DPN) 1 a las 21 3 horas en la mañana y 3 en la tarde. El DPN 22 se destetaron, separaron por sexo y se brindó alimentación y agua ad-libitum; el DPN 27- 52 se ofreció consumo de edulcorante (sucralosa) y agua; el DPN 53 se sacrificaron con perfusión transcardiaca, remoción de encéfalo y posteriormente se realizó el corte e inmunohistoquímica en áreas seleccionadas, toma de microfotografía, medición de densitometría y análisis estadístico (test Kruskall Wallis; software R, P= 0.05). Resultados: existen cambios diferenciales en la expresión de receptores cannabinoides CB1 y opioides tipo Mu en áreas analizadas en condiciones estándar de crianza y alimentación, así mismo dicha expresión tiende a alterarse de manera diferencial dependiente del sexo posterior a la SMDL y el consumo de sucralosa de manera independiente y conjunta, tendientes a aumentar su expresión en machos y disminuirla en hembras. Conclusión: La SMDL genera alteración en receptores CB1 y Mu en areas neuromoduladores del eje HPA y sistema de recompensa; así mismo, el posterior consumo de sucralosa exacerba el efecto de la SMDL. (Texto tomado de la fuente).spa
dc.description.abstractMaternal separation during breastfeeding (MSDB) in rodents (chronic stress model) alters developmental processes at neuroendocrine and physiological levels, evident in adolescence and adulthood associated with depression, substance abuse, learning difficulties, anxiety and eating disorders. In this sense, endocannabinoid and opioid neuromodulatory systems that act on the activity of the hypothalamic-pituitary-adrenal axis and affect regulatory areas of eating behavior and reward system through the action of CB1 cannabinoid receptors and Mu-type opioids could present alterations. Therefore, the aim of this research was to determine the effect of MSDB and sucralose consumption on the expression of CB1 and Mu receptors in the medial prefrontal cortex, hippocampus, striatum, ventral tegmental area and amygdala of adolescent wistar rats of both sexes. Methodology: 35 male and female Wistar rats divided into groups with and without MSDB (n=18; n:17) were used during postnatal days (PND) 1 at 21 3 hours in the morning and 3 in the afternoon. On PND 22 they were weaned, separated by sex and fed and water was provided ad-libitum; on PND 27- 52 they were offered sweetener consumption (sucralose) and water; on PND 53 they were sacrificed with transcardiac perfusion, removal of the encephalon and then cut and immunohistochemistry was performed in selected areas, microphotography, densitometry measurement and statistical analysis (Kruskall Wallis test; R software, P= 0.05). Results: there are differential changes in the expression of CB1 cannabinoid and Mu-type opioid receptors in areas analyzed under standard rearing and feeding conditions. Likewise, such expression tends to alter in a sex-dependent differential manner after MSDB and sucralose consumption independently and jointly, tending to increase its expression in males and decrease it in females. Conclusion: MSDB generates alterations in CB1 and Mu receptors in neuromodulatory areas of the HPA axis and reward system and the subsequent sucralose consumption exacerbates the effect of MSDB.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Fisiologíaspa
dc.description.researchareaNeurofisiología comportamentalspa
dc.format.extentxi, 84 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83973
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Medicina - Maestría en Fisiologíaspa
dc.relation.indexedBiremespa
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dc.relation.referencesSuez, J., Korem, T., Zeevi, D., Zilberman-Schapira, G., Thaiss, C. A., Maza, O., Israeli, D., Zmora, N., Gilad, S., Weinberger, A., Kuperman, Y., Harmelin, A., Kolodkin-Gal, I., Shapiro, H., Halpern, Z., Segal, E., & Elinav, E. (2014). Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature, 514(7521), 181-186. https://doi.org/10.1038/nature13793spa
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dc.relation.referencesThomas, A. W., Caporale, N., Wu, C., & Wilbrecht, L. (2016). Early maternal separation impacts cognitive flexibility at the age of first independence in mice. Developmental Cognitive Neuroscience, 18, 49-56. https://doi.org/10.1016/j.dcn.2015.09.005spa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animalesspa
dc.subject.decsPrivación Maternaspa
dc.subject.decsMaternal Deprivationeng
dc.subject.decsReceptores de Cannabinoides/fisiologíaspa
dc.subject.decsReceptors, Cannabinoid/physiologyeng
dc.subject.decsReceptores Opioides/fisiologíaspa
dc.subject.decsReceptors, Opioid/physiologyeng
dc.subject.proposalSeparación materna durante la lactanciaspa
dc.subject.proposalReceptor opioide Muspa
dc.subject.proposalEarly life stresseng
dc.subject.proposalCB1 Receptoreng
dc.subject.proposalMu opioid receptoreng
dc.subject.proposalReceptor CB1spa
dc.subject.proposalSucralosaspa
dc.subject.proposalEstrésspa
dc.subject.proposalEpigenéticaspa
dc.subject.proposalObesidadspa
dc.subject.proposalSucraloseeng
dc.subject.proposalObesityeng
dc.titleEfecto de la separación materna durante la lactancia y el consumo de sucralosa sobre la expresión de receptores de endocanabinoides y de receptores de opioides endógenos en cerebros de ratasspa
dc.title.translatedEffect of maternal separation during breastfeeding and sucralose consumption on endocannabinoid receptors and endogenous opioid receptors expression´s in rat brainseng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
dcterms.audience.professionaldevelopmentMedios de comunicaciónspa
dcterms.audience.professionaldevelopmentPadres y familiasspa
dcterms.audience.professionaldevelopmentPersonal de apoyo escolarspa
dcterms.audience.professionaldevelopmentPúblico generalspa
dcterms.audience.professionaldevelopmentResponsables políticosspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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