Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida

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dc.contributor.advisorGuerrero Fonseca, Carlos Arturo
dc.contributor.authorRojas Hernández, Laura Camila
dc.contributor.researchgroupBiología celular y funcional e ingeniería de biomoléculasspa
dc.date.accessioned2024-04-30T16:53:18Z
dc.date.available2024-04-30T16:53:18Z
dc.date.issued2023
dc.descriptionilustraciones, diagramas, fotografíasspa
dc.description.abstractLos trastornos neurológicos son enfermedades degenerativas del sistema nervioso central (SNC) y constituyen una causa frecuente de morbilidad y mortalidad en el mundo, y han incrementado en los últimos años. Las nanopartículas USPIO (Ultrasmall Superparamagnetic Iron Oxide) son una categoría novedosa de agentes de contraste en resonancia magnética de imagen (RMI) que pueden acoplarse a diversas moléculas y dirigirse de manera específica hacia marcadores moleculares que reflejen alteraciones específicas. Por lo anterior, el objetivo del presente estudio fue caracterizar el efecto de las nanopartículas de USPIO conjugadas con el péptido marcador P88 en un modelo de neuroinflamación inducida in vitro con potencial aplicabilidad en RMI. Para lograr este objetivo, las líneas celulares HCMEC/D3 (células endoteliales de microvasculatura de cerebro) y T98G (astrocitos) se incubaron USPIO conjugadas con el péptido marcador y se determinó su efecto sobre la viabilidad celular por el método de LDH. Posteriormente, se identificó el perfil inflamatorio por medio de citometría de flujo y ensayos de PCR en tiempo real. Finalmente, se determinó el perfil de estrés oxidativo mediante la medición de los niveles de superóxido producidos. En general, los resultados de este estudio sugieren que NP88 es biocompatible con las líneas celulares HCMEC/D3 y T98G; y se podría utilizar potencialmente como biomarcador en modelos de neuroinflamación (Texto tomado de la fuente)spa
dc.description.abstractNeurological disorders are degenerative diseases of the central nervous system (CNS) and constitute a frequent cause of morbidity and mortality in the world, and have increased in recent years. USPIO (Ultrasmall Superparamagnetic Iron Oxide) nanoparticles are a novel category of magnetic resonance imaging (MRI) contrast agents that can couple to various molecules and specifically target molecular markers that reflect specific abnormalities. Therefore, the objective of this study was to characterize the effect of USPIO nanoparticles conjugated with the marker peptide P88 in an in vitro induced neuroinflammation model with potential applicability in MRI. To achieve this goal, the cell lines HCMEC/D3 (brain microvasculature endothelial cells) and T98G (astrocytes) were incubated USPIO conjugated with the marker peptide and their effect on cell capacity was amplified by the LDH method. Subsequently, the inflammatory profile was identified by means of flow cytometry and real-time PCR assays. Finally, the oxidative stress profile was reduced by measuring the levels of superoxides produced. Overall, the results of this study suggest that NP88 is biocompatible with the HCMEC/D3 and T98G cell lines; and could potentially be used as a biomarker in models of neuroinflammationeng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Bioquímicaspa
dc.format.extent83 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/86003
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Medicina - Maestría en Bioquímicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.decsEnfermedades Neurodegenerativasspa
dc.subject.decsNeurodegenerative Diseaseseng
dc.subject.proposalImágenes de resonancia magnéticaspa
dc.subject.proposalNanopartículas de USPIOspa
dc.subject.proposalBiomarcadorspa
dc.subject.proposalMagnetic resonance imagingeng
dc.subject.proposalUSPIO nanoparticleseng
dc.titleDeterminación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducidaspa
dc.title.translatedIn vitro determination of the biocompatibility of a marker peptide coupled to USPIO nanoparticles in an induced neuroinflammation model.eng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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