Elucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tn

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dc.contributor.advisorVega Castro, Nohora Angélica
dc.contributor.authorEspinosa Velandia, Jessica Natalia
dc.contributor.researchgroupGrupo de Investigación en Proteínas GRIPspa
dc.date.accessioned2022-08-25T16:46:45Z
dc.date.available2022-08-25T16:46:45Z
dc.date.issued2022
dc.descriptionilustraciones, fotografías, graficasspa
dc.description.abstractEl estudio de las lectinas vegetales ha sido de gran importancia debido a su capacidad de reconocer residuos de carbohidratos, los cuales han sido llamados glicotopes; alguno de ellos se encuentra enmascarados en la superficie celular y están asociados con una gran variedad de carcinomas. Algunas de las lectinas que más se han estudiado son las de origen vegetal de la familia Leguminosae, sin embargo, también se han aislado de otras familias botánicas, tales como Lamiácea. Las lectinas de Lamiáceas se caracterizan porque reconocen los glicotopes T y/o Tn. La información disponible acerca de estas proteínas es muy escasa debido a que los procesos de purificación no permiten obtener la cantidad necesaria para realizar estudios de caracterización estructural y de interacción con el antígeno Tn/T; por otra parte, hasta el momento solo hay dos secuencias de especies relacionadas obtenidas a partir del cDNA, la lectina de Salvia miltorriza (LSM) y de Gleheda y solo se han caracterizado cuatro lectinas de la zona de Eurasia y dos lectinas de especies endémicas de Colombia, la lectina de Salvia bogotensis (LSBo-I) y de Lepechinia bullata (LLb-I) En el presente trabajo, se realizó la purificación de la lectina de Salvia bogotensis I (LSBo-I), empleando cromatografía de intercambio iónico y afinidad. Las fracciones obtenidas presentaron actividad aglutinante por eritrocitos Tn y se observó por SDS PAGE, una banda alrededor de 36 kDa por SDS-PAGE que es similar a lo obtenido en estudios previos. La sepharosa 4B, fue acoplada a una IgG de conejo (antiLSBo-I), y la elución se llevó a cabo mediante un gradiente de pH. En cada etapa de purificación se evaluó la actividad aglutinante con eritrocitos humanos A+, los cuales fueron tratados enzimáticamente con neuraminidasa y B- galactosidasa para exponer el Tn. Se obtuvo el 71% de la secuencia y por predicción se determinó que pertenece a las lectinas tipo Leguminosa (L). También se llevaron a cabo ensayos para la obtención del ADNc y la secuencia de nucleótidos de la LSBo-I. (Texto tomado de la fuente)spa
dc.description.abstractPlant lectins studies have been of great importance due to their ability to recognize carbohydrate residues on surface cells, epitopes-like glycotopes are called. Some of them are masked on the cell surface and are associated with a wide variety of carcinomas. The most studied lectins belong to the Leguminosae family however, they have also been isolated from other botanical families, such as Lamiaceae. Their lectins are characterized by recognizing T and/or Tn glycotypes. The information available about these proteins is very scarce owing to purification processes do not allow obtaining the amount to carry out structural studies and interaction with Tn/T glycans. Secondly, there are only two sequences related to Lamiacea lectins, Salvia miltorriza (SML) and Gleheda and four lectins have been studied from Eurasia zone and two endemic lectins from Colombia, Salvia bogotensis (SBoL-I) and Lepechinia bullata (LBL-I). In this work, Ion-exchange and affinity chromatography’s led to getting SBoL-I that showed only a band by SDS PAGE, around 36 kDa, according to previous studies. Main purification step was carried out using affinity chromatography, a Sepharose 4B was coupled to IgG (antiSBoL-I) and pH gradient elution. The agglutinating activity was tested using A+ human erythrocytes, which were enzymatically treated with neuraminidase and B−galactosidase, to expose Tn structures. Finally, a sequence with 71% coverage was obtained and three-dimensional model type of Leguminous was predicted by homology. Additionally, cDNA assays were carried out to get nucleotide sequence from SBoL-I.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Bioquímicaspa
dc.description.researchareaLectinas de Lamiaceaspa
dc.format.extentxix, 126 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/82105
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.departmentDepartamento de Químicaspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Bioquímicaspa
dc.relation.indexedRedColspa
dc.relation.indexedLaReferenciaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.agrovocLectinasspa
dc.subject.agrovoclectinseng
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.lembANTIGENOS DE TUMORES VIRALESspa
dc.subject.lembAntigens viral tumoreng
dc.subject.proposalLectina vegetalspa
dc.subject.proposalSalvia bogotensisspa
dc.subject.proposalFamilia Lamiaceaespa
dc.subject.proposalGlicotopes T y Tnspa
dc.subject.proposalLectinas tipo L.spa
dc.subject.proposalVegetal lectineng
dc.subject.proposalLamiaceae familyeng
dc.subject.proposalT and Tn glycotopeeng
dc.subject.proposalL-type lectinseng
dc.titleElucidación de la estructura primaria de la lectina de Salvia bogotensis (LSBo-I) específica para la detección de los antígenos T y Tnspa
dc.title.translatedElucidation of the primary structure of the Salvia bogotensis lectin (LSBo-I) specific for the detection of T and Tn antigeneng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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