Péptidos quiméricos derivados de Lactoferricina Bovina y Buforina II: actividad antifúngica contra aislados clínicos y cepas de referencia de Candida spp

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dc.contributor.advisorParra Giraldo, Claudia Marcela
dc.contributor.authorAguirre Guataqui, Katherine Natalia
dc.contributor.authorParra Giraldo, Claudia Marcela
dc.contributor.authorGarcía Castañeda, Javier Eduardo
dc.contributor.cvlacKatherine Aguirre-Guataqui 0001665676spa
dc.contributor.educationalvalidatorYerly Vargas-Casanova
dc.contributor.educationalvalidatorAndrés Ceballos-Garzon
dc.contributor.researchgateKatherine-Aguirre-Guataquispa
dc.contributor.researchgroupSíntesis y Aplicación de Moléculas Peptídicasspa
dc.contributor.researchgroupUnidad de Investigación en Proteómica y micosis Humanas-PUJspa
dc.contributor.scopusKatherine Aguirre-Guataqui 0000-0002-9122-678Xspa
dc.date.accessioned2023-02-17T13:05:13Z
dc.date.available2023-02-17T13:05:13Z
dc.date.issued2022-10-14
dc.descriptionilustraciones, fotografías a colorspa
dc.description.abstractEl número reducido de moléculas con actividad antifúngica y la aparición de aislamientos clínicos resistentes como las levaduras del género Candida spp., ha limitado la eficacia terapéutica de antifúngicos en los últimos años. En este trabajo examinamos los efectos de PAM quiméricos que contienen el motivo mínimo de LfcinB y BFII en C. albicans (CAAL), C. glabrata (CAGL) y C. auris (CAAU), con la evaluación de la concentración mínima inhibitoria (CMI) y concentración mínima fungicida (CMF), cinética de crecimiento y la combinación de la quimera QC2 con FLU. Nuestros resultados indicaron que las quimeras QC1 y QC2 exhibieron actividad fungistática y fungicida dependiente de la concentración contra Candida spp resistente, mayor actividad en comparación con el motivo mínimo de LfcinB, el palíndromo de BFII y las otras cinco quimeras estudiadas; y un efecto de aditividad con fluconazol contra CAAL256 (resistente a FLU), C. glabrata 2001 y C. auris 001. Estas quimeras pueden ser consideradas promisorias ya que además de presentar actividad antifúngica contra cepas de referencia y aislados clínicos de Candida spp, también se ha reportado que exhiben actividad antibacteriana, tanto en bacterias Gram positivas como Gram negativas(1). Los resultados sugieren que estas quimeras pueden ser de amplio espectro antimicrobiano. (Texto tomado de la fuente)spa
dc.description.abstractThe reduced number of molecules with antifungal activity and the appearance of resistant clinical isolates, such as yeasts of the genus Candida spp., have limited the therapeutic efficacy of antifungal agents in recent years. In this work, we examined the effects of chimeric PAMs containing the minimal motif of LfcinB and BFII in C. albicans (CAAL), C. glabrata (CAGL), and C. auris (CAAU), with the evaluation of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), growth kinetics, and combination of QC2 chimera with FLU. Our results indicated that QC1 and QC2 chimeras exhibited concentration-dependent fungistatic and fungicidal activity against resistant Candida spp, higher activity compared to minimal motifs of LfcinB, BFII palindrome and other five chimeras studied, and an additivity effect with fluconazole against CAAL256 (resistant to FLU), C. glabrata 2001 and C. auris 001. In addition to present antifungal activity against reference strains and clinical isolates of Candida spp, they also presented antibacterial activity, both in Gram-positive and Gram-negative bacteria (1). These chimeras show a broad antimicrobial spectrum and can be considered as promising molecules for therapeutic applications.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagister en Ciencias--Microbiologíaspa
dc.format.extentxii, 93 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83512
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.decsPéptidosspa
dc.subject.decsPeptideseng
dc.subject.decsAnálisisspa
dc.subject.decsAnalysiseng
dc.subject.proposalCandida albicansspa
dc.subject.proposalCandida glabrataspa
dc.subject.proposalCandida aurisaps
dc.subject.proposalBovine lactoferricineng
dc.subject.proposalBuforin IIeng
dc.subject.proposalAntimicrobial peptideseng
dc.subject.proposalChimeraseng
dc.titlePéptidos quiméricos derivados de Lactoferricina Bovina y Buforina II: actividad antifúngica contra aislados clínicos y cepas de referencia de Candida sppspa
dc.title.translatedChimeric peptides derived from Bovine Lactoferricin and Buforin II: antifungal activity against clinical isolates and reference strains of Candida sppeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentOtherspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleDiseño de una formulación para el tratamiento de la candidiasis invasiva multidrogo resistente, basada en péptidos de LfcinB libres o nanoencapsulados.spa
oaire.fundernameCOLCIENCIASspa

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Tesis de Maestría en Ciencias - Microbiología
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Maestría en Ciencias - Microbiología