Efecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthi

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dc.contributor.advisorArdila Barrantes, Harold Duban
dc.contributor.authorCastiblanco Quiroga, Nelly Fernanda
dc.contributor.researchgroupEstudio de Actividades Metabólicas Vegetalesspa
dc.date.accessioned2023-06-14T23:13:05Z
dc.date.available2023-06-14T23:13:05Z
dc.date.issued2023-06-15
dc.descriptionilustraciones, fotografías a colorspa
dc.description.abstractLa tiamina se ha postulado como un potencial inductor de resistencia al marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi (Fod), dado que su aspersión foliar en plantas de clavel reduce significativamente la severidad de la enfermedad. No obstante, hasta el momento se desconocen los fenómenos bioquímicos involucrados en la inducción de resistencia en este modelo, por ejemplo, no se conoce el efecto que puede tener este tipo de sustancias directamente sobre el patógeno. En este estudio se investigó el efecto que tiene la tiamina sobre el crecimiento micelial y los niveles transcripcionales de los genes sod1, sed4, six7 y six10 los cuales codifican para posibles factores de virulencia de este patógeno del clavel. Para ello, primero se realizó el cultivo in vitro del patógeno y se determinó el efecto de la presencia de tiamina sobre el crecimiento y los niveles de transcripción de sod1 y sed4. Posteriormente, se inocularon con Fod plantas de clavel previamente tratadas con tiamina y se confirmó el efecto de ésta en la disminución de la severidad a la enfermedad; en este punto se evaluaron los niveles de transcripción in planta de los genes sed4, sod1, six7 y six10 en dos variedades de clavel con niveles de resistencia contrastante al marchitamiento vascular. En la primera etapa, se evidenció que, durante la fase exponencial, la presencia de tiamina genera una disminución de hasta un 68% en el crecimiento micelial, al comparar con el control; así mismo se observó que la presencia de este compuesto genera una modulación de los niveles transcripcionales de sod1 y sed4 en función de la fase de crecimiento del patógeno. A nivel in planta, se confirmó que la aspersión foliar de tiamina disminuye la severidad de la enfermedad causada por Fod y que, durante este proceso, hay una disminución de los niveles de expresión de sod1, six7, six10 y un aumento de la expresión de sed4. Además, se observó que los niveles transcripcionales de estos genes se encuentran modulados diferencialmente en función del nivel de resistencia de la planta. Estos hallazgos sugieren que la tiamina actúa como potencial inhibidor del crecimiento micelial in vitro y como un regulador de la transcripción de diversos genes relacionados con virulencia en Fusarium oxysporum f. sp. dianthi. (Texto tomado de la fuente)spa
dc.description.abstractThiamine has been proposed as a potential inducer of resistance to vascular wilt caused by Fusarium oxysporum f. sp. dianthi (Fod), since its foliar spraying on carnation plants results in a significant reduction in the severity of the disease. However, the biochemical phenomena involved in the induction of resistance in this model are still unknown: for example, the effect that this type of substance can have directly on the pathogen is also unknown. In this study, we investigate the effect of thiamine on mycelial growth and transcriptional levels of the sod1, sed4, six7 and six10 genes, which are encoding potential virulence factors of this carnation pathogen. For that purpose, the pathogen was first cultured in vitro and the effect of the presence of thiamine on the growth and transcriptional levels of sod1 and sed4 was evaluated. Subsequently, carnation plants previously treated with thiamine were inoculated with Fod and the effect of thiamine on the reduction of disease severity was confirmed; at this point, the in planta transcription levels of the sed4, sod1, six7 and six10 genes were analyzed in two carnation varieties with contrasting levels of resistance to vascular wilt. In the first stage, it was found that, during the exponential phase, the presence of thiamine generated a decrease of up to 68% in mycelial growth, when compared to the control; it was also observed that the presence of this compound generated a modulation of the transcriptional levels of sod1 and sed4 depending on the growth phase of the pathogen. At the in planta level, it was confirmed that the foliar spray of thiamine reduces the severity of the disease caused by Fod and that during this process, there is a decrease in the expression levels of sod1, six7, six10 and an increase in the expression of sed4. Furthermore, the transcriptional levels of these genes were found to be differentially modulated depending on the level of plant resistance. These findings suggested that thiamine acts as a potential inhibitor of mycelial growth in vitro and as a transcriptional regulator of several virulence-related genes in Fusarium oxysporum f. sp. dianthi.eng
dc.description.degreelevelMaestríaspa
dc.description.researchareaInteracción Hospedero - Patógenospa
dc.format.extentxxiii,110 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84021
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá,Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Bioquímicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.ddc580 - Plantasspa
dc.subject.ddc540 - Química y ciencias afinesspa
dc.subject.ddc580 - Plantas::582 - Plantas destacadas por características vegetativas y floresspa
dc.subject.lembClavelesspa
dc.subject.lembCarnationseng
dc.subject.lembVirus fitopatógenosspa
dc.subject.lembPlant viruseseng
dc.subject.lembVirosis (plantas)spa
dc.subject.lembVirus diseases of plantseng
dc.subject.proposalFusarium oxysporum f. sp. dianthispa
dc.subject.proposalDianthus caryophyllus L.spa
dc.subject.proposalTiaminaspa
dc.subject.proposalSuperóxido dismutasaspa
dc.subject.proposalTripeptidil peptidasaspa
dc.subject.proposalProteínas SIXspa
dc.subject.proposalFactor de virulenciaspa
dc.subject.proposalFusarium oxysporum f. sp. dianthieng
dc.subject.proposalDianthus caryophyllus L.eng
dc.subject.proposalThiamineeng
dc.subject.proposalSuperoxide dismutaseeng
dc.subject.proposalTripeptidyl peptidaseeng
dc.subject.proposalSIX proteinseng
dc.subject.proposalVirulence factoreng
dc.titleEfecto de la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L) sobre la expresión in vivo de genes codificantes para candidatos a factores de virulencia del patógeno Fusarium oxysporum f. sp. dianthispa
dc.title.translatedEffect of the application of resistance inducers on the in vivo expression of candidate virulence factor genes of the pathogen Fusarium oxysporum f. sp. dianthi in carnation (Dianthus caryophyllus L)eng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.fundernameMinCienciasspa

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